BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	431,128	G→A	A134V (GCA→GTA)	JCVSYN2_01955 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	431,128	0	G	A	99.7%	10464.3 / NA	3265	A134V (GCA→GTA)	JCVSYN2_01955	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/1); major base A (1796/1455); minor base T (3/6); total (1803/1462)
Fisher's exact test for biased strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3266 total aligned reads displayed.

TTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAATTCAATCTTTTTTGCT  >  CP016816/431003‑431252

     aaaaTTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTg                                                                                                                       >  1:6177028/1‑128 (MQ=255)

     aaaaTTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTg                                                                                                                       <  2:16682/128‑1 (MQ=255)

       aaTTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTa                                                                                                                     <  1:5243604/128‑1 (MQ=255)

       aaTTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTa                                                                                                                     <  2:6968237/128‑1 (MQ=255)

         ttAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGt                                                                                                                   >  1:7001426/1‑128 (MQ=255)

           aaaaaGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTgg                                                                                                                 >  1:1018175/1‑128 (MQ=255)

             aaaGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGat                                                                                                               >  1:6709775/1‑128 (MQ=255)

                 aCTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATaa                                                                                                           >  1:1124403/1‑128 (MQ=255)

                  cTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTATTTAGTGGAGAAATTACAATTTGTAGTGGATATaaa                                                                                                          >  2:2277781/1‑128 (MQ=255)

                  cTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATaaa                                                                                                          >  2:1139754/1‑128 (MQ=255)

                  cTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATaaa                                                                                                          >  1:1256124/1‑128 (MQ=255)

                  cTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATaaa                                                                                                          >  2:5761512/1‑128 (MQ=255)

                  cTGCACTTAATTCTCAAAAATTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATaaa                                                                                                          <  2:4457113/128‑1 (MQ=255)

                      aCTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAg                                                                                                      <  1:6305265/128‑1 (MQ=255)

                      aCTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAg                                                                                                      >  1:406143/1‑128 (MQ=255)

                      aCTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAg                                                                                                      >  2:4825381/1‑128 (MQ=255)

                        ttAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGat                                                                                                    <  1:1884250/128‑1 (MQ=255)

                           aTTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGataat                                                                                                 >  1:3436134/1‑128 (MQ=255)

                            ttCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAAt                                                                                                        <  1:2811966/120‑1 (MQ=255)

                              ctcAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAAGTTGTAGTGGATATAAAATAGATAATaaa                                                                                              >  1:3164823/1‑128 (MQ=255)

                              ctcAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGATTAGAAATTACAATATGTAGTGGTTATAAAATAGataatata                                                                                              >  2:1917779/1‑126 (MQ=255)

                              ctcAAAAATATAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATaaa                                                                                              <  1:1616318/128‑1 (MQ=255)

                                cAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATaaaaa                                                                                            >  2:2803125/1‑128 (MQ=255)

                                  aaaCTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATaaaaaaa                                                                                          >  2:2399498/1‑128 (MQ=255)

                                   aaCTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAAc                                                                                         <  2:5544115/128‑1 (MQ=255)

                                   aaCTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAAc                                                                                         >  2:3177060/1‑128 (MQ=255)

                                   aaCTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTATAGTGGATATAAAATAGATTATaaaaataa                                                                                         >  1:5120563/1‑125 (MQ=255)

                                     cTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTa                                                                                       >  2:29236/1‑128 (MQ=255)

                                     cTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTa                                                                                       >  1:885128/1‑128 (MQ=255)

                                      tttAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTTATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTaa                                                                                      >  1:5397333/1‑128 (MQ=255)

                                      tttAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTaa                                                                                      >  2:2025760/1‑128 (MQ=255)

                                      tttAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTaa                                                                                      >  1:3540085/1‑128 (MQ=255)

                                        tAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTaaaa                                                                                    >  1:3056515/1‑128 (MQ=255)

                                           aGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAaaataa                                                                                 <  2:3802662/128‑1 (MQ=255)

                                             tAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAaaataaat                                                                               >  2:5911035/1‑128 (MQ=255)

                                             tAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAaaataaat                                                                               >  2:6546679/1‑128 (MQ=255)

                                               gAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATcc                                                                             >  1:5955508/1‑128 (MQ=255)

                                               gAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATca                                                                             >  1:2679076/1‑127 (MQ=255)

                                               gAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTCGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATcc                                                                             >  2:3307604/1‑128 (MQ=255)

                                                aaGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCa                                                                            <  2:4691843/128‑1 (MQ=255)

                                                aaGTTGTTTTAGATATAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCa                                                                            <  2:1803471/128‑1 (MQ=255)

                                                 aGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAg                                                                           >  1:147923/1‑128 (MQ=255)

                                                    tgttTTTGATAAAGGTGTAGTAAAATCAATTCATCATTTAATTGATTCACCACTATTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAACAAACTAAAATAAATCCAGaaa                                                                        >  1:6455121/1‑128 (MQ=255)

                                                      tttttGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAATCa                                                                      >  2:3065337/1‑128 (MQ=255)

                                                       ttatGATTAATGTGTAGTATAATCAATTCCTCATTTAATTGATTCAACAATAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACaa                                                                     <  2:2700840/125‑1 (MQ=255)

                                                         ttGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAAtt                                                                   >  1:1739319/1‑128 (MQ=255)

                                                           gATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCAACACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAAtttt                                                                 <  2:1385915/128‑1 (MQ=255)

                                                            ataatagtgtATTAAAATTAATTTCTCATTTAATTTATTTACCAATATTAATTATGTGAGAAATTAAAATTTTTAGTGGATATAAAATATATAATAAAAAAACTAAAATAAATCCAGAAACAATTtta                                                                <  1:2268564/122‑1 (MQ=255)

                                                             tAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGAAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCAAGCAACAATTtaat                                                               >  2:4188098/1‑128 (MQ=255)

                                                                 ggTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATAAGTGGAGAAATTACAATTTGTAGTGTATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAAAAATTttattata                                                           >  1:6844231/1‑127 (MQ=255)

                                                                  gtgtAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTttattatta                                                          <  2:6153647/128‑1 (MQ=255)

                                                                   tgtAGTAAAATCTATTCCTCATTTAATTGATTCACAACTAGTAATTAGAGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTtattattat                                                         <  2:7137816/128‑1 (MQ=255)

                                                                   tgtAGTAAAATCAATTCCTAATTTAATTGTTTCACCAATAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATTAATCCAGAAACAATTTtattattat                                                         <  1:4748898/128‑1 (MQ=255)

                                                                        taAAATCAATTCCTCATTTAATTGATTCATCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAAttt                                                    >  2:4040334/1‑128 (MQ=255)

                                                                         aaaaTCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTc                                                   <  1:2562554/128‑1 (MQ=255)

                                                                           aaTCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCtt                                                 >  1:766438/1‑128 (MQ=255)

                                                                           aaTCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCtt                                                 <  1:5001213/128‑1 (MQ=255)

                                                                            aTCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAAATTTATTATTATAATTTCatt                                                >  2:867713/1‑128 (MQ=255)

                                                                              catttCCTGATTTAATTGATTCACCTATAGTAATTAGTTGAGAAATTAACATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCttttt                                              <  2:1545650/125‑1 (MQ=255)

                                                                              cAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCttttt                                              <  1:2163862/128‑1 (MQ=255)

                                                                              cAATTCCTCATTTAATTGATTCAAAACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAATATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCttttt                                              <  2:5266714/128‑1 (MQ=255)

                                                                               aaTTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAaaat                                                                                   >  2:1246950/1‑90 (MQ=255)

                                                                                aTTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCtttttcc                                            >  2:6708062/1‑126 (MQ=255)

                                                                                aTTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGc                                            <  1:3835692/128‑1 (MQ=255)

                                                                                aTTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGAGATAAAATAGATAATAAAAAAGCTAAAATAAATCCAGAAACAATTTTATTATAATATTTTCTTTTTGc                                            >  1:4614468/1‑128 (MQ=255)

                                                                                 ttCCTCATTTAATTTATTCAAAACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCa                                           <  2:4964399/128‑1 (MQ=255)

                                                                                  tCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAg                                          >  2:3681072/1‑128 (MQ=255)

                                                                                     tCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAATAAAACTAAAATAAATCCAGAAACAATATTATTATTATAATTTCTTTTTGCAGTTg                                       >  2:6427254/1‑128 (MQ=255)

                                                                                     tCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAGTTTTATTATTATAATTTCTTTTTGCAGTTg                                       >  1:3664687/1‑128 (MQ=255)

                                                                                      cATTTAATTGATTCACCAATAGTACTTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGa                                      <  2:4557642/128‑1 (MQ=255)

                                                                                       aTTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGaa                                     >  1:4368150/1‑128 (MQ=255)

                                                                                       aTTTAATTGATTCACAACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGaa                                     <  2:3469325/128‑1 (MQ=255)

                                                                                          tAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGaaaaa                                  <  1:5685978/128‑1 (MQ=255)

                                                                                          tAATTGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGaaaaa                                  <  1:327712/128‑1 (MQ=255)

                                                                                             ttGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAAtt                               <  2:5394100/128‑1 (MQ=255)

                                                                                             ttGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAAtt                               <  1:4890630/128‑1 (MQ=255)

                                                                                             ttGATTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTAGAAAAAAtt                               >  1:1348515/1‑128 (MQ=255)

                                                                                                aTTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCa                            <  1:71166/128‑1 (MQ=255)

                                                                                                aTTCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCa                            <  2:1035347/128‑1 (MQ=255)

                                                                                                 ttCACCACTAGTAATTAGTGGAGAAATTACAATTTGTAGTTGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGCAACAATTTTATAATTATAATTTCTTTTTGCATTTTAAAAAATATCAt                           >  1:2420160/1‑128 (MQ=255)

                                                                                                   caccacTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTa                         >  2:2582717/1‑128 (MQ=255)

                                                                                                       acTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTg                     >  2:6043989/1‑128 (MQ=255)

                                                                                                        cTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGt                    <  1:4201877/128‑1 (MQ=255)

                                                                                                        cTAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGt                    <  1:580281/128‑1 (MQ=255)

                                                                                                         tAGTAATTAGTTGAGAAATTACAATTTGTAGTGGTTATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTa                   <  1:2941759/128‑1 (MQ=255)

                                                                                                         tAGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTa                   >  1:447715/1‑128 (MQ=255)

                                                                                                          aGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTaa                  >  2:3567266/1‑128 (MQ=255)

                                                                                                          aGTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTaa                  >  2:5094405/1‑128 (MQ=255)

                                                                                                           gTAATTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAAt                 <  1:2038151/128‑1 (MQ=255)

                                                                                                             aaTTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAATATTTCATTAACTGTAATTc               >  2:6845249/1‑128 (MQ=255)

                                                                                                             aaTTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAATTc               >  2:5659477/1‑128 (MQ=255)

                                                                                                              aTTAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGt                    <  1:6578869/122‑1 (MQ=255)

                                                                                                               ttAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATACAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAATTCaa             <  1:3965191/128‑1 (MQ=255)

                                                                                                                tAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAATTCAAt            >  2:6270518/1‑128 (MQ=255)

                                                                                                                tAGTGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAACCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAATTCAAt            <  1:5522521/128‑1 (MQ=255)

                                                                                                                   tGGAGAAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAATTCAATCtt         >  1:6062061/1‑128 (MQ=255)

                                                                                                                     gagaTATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATACAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAATTCAATCtttt       <  2:2131367/128‑1 (MQ=255)

                                                                                                                     gagaAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAATTCAATCtttt       >  2:4364646/1‑128 (MQ=255)

                                                                                                                      agaAATTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCtttt                                               <  2:3955682/87‑1 (MQ=255)

                                                                                                                          aTTACAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAATTCAATCTTTTTTGCt  <  2:766438/128‑1 (MQ=255)

TTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAATTCAATCTTTTTTGCT  >  CP016816/431003‑431252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`