Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,801 A→G intergenic (‑110/+61) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,8010AG98.0% 11995.3 / NA 3325intergenic (‑110/+61)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (17/1);  major base G (1554/1675);  minor base T (45/20);  total (1629/1696)
Fisher's exact test for biased strand distribution p-value = 9.71e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3680 total aligned reads displayed.

AAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484674‑484925
                                                                                                                               |                                                                                                                            
  aaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAATCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCa                                                                                                                            >  1:5215090/1‑128 (MQ=255)
    tAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGAATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                                          <  1:2235015/128‑1 (MQ=255)
        aaaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGATCATAGCAGAATATCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                                      <  2:4494589/127‑1 (MQ=255)
          aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                                    >  2:6986210/1‑128 (MQ=255)
          aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                                    >  2:6805179/1‑128 (MQ=255)
          aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                                    <  1:2615020/128‑1 (MQ=255)
           aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                                   >  1:3914542/1‑128 (MQ=255)
           aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                                   >  2:1331062/1‑128 (MQ=255)
             cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                                 >  1:6071087/1‑128 (MQ=255)
             cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                                 <  1:5792009/128‑1 (MQ=255)
               cATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaa                                                                                                               >  2:1910461/1‑128 (MQ=255)
                aTAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaa                                                                                                              >  2:120489/1‑128 (MQ=255)
                   gATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                                           <  1:1156904/128‑1 (MQ=255)
                      ttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGAATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                                        <  2:2844156/128‑1 (MQ=255)
                        aaTACTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcc                                                                                                      <  1:1741904/128‑1 (MQ=255)
                             ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                                 >  2:6290812/1‑128 (MQ=255)
                             ttttATAGTAGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                                 <  2:5001049/128‑1 (MQ=255)
                              tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTaa                                                                                                >  1:6538341/1‑127 (MQ=255)
                               ttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                               >  1:3095972/1‑128 (MQ=255)
                                tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                              >  2:6165469/1‑128 (MQ=255)
                                tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGATCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAACCCATTATgg                                                                                              >  2:6590084/1‑128 (MQ=255)
                                 ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                             <  2:645317/128‑1 (MQ=255)
                                 ataGATGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                             <  2:331942/128‑1 (MQ=255)
                                   aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                           <  2:162958/128‑1 (MQ=255)
                                        cccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCCATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATAAAAAACACAAAAACTCACCAATTAGGTTGAttttg                                                                                      >  1:1262328/2‑127 (MQ=255)
                                        aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                                      <  2:4135992/128‑1 (MQ=255)
                                         ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                                     <  2:2439538/128‑1 (MQ=255)
                                          cAAAAAATAAGATAGATCATAGCAGTATCTCTTAACAAATAAACTACAATAGATTATTTATTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaa                                                                                    <  1:1977252/128‑1 (MQ=255)
                                          cAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAATATGGTGAGTTTaaa                                                                                    >  1:456662/1‑128 (MQ=255)
                                           aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACATCTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTTTCGTGAGTTTaaaa                                                                                   >  2:5577166/1‑128 (MQ=255)
                                            aaaatTAAGATAGAGCATAGCAGAAGCTCATAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  1:1005954/128‑1 (MQ=255)
                                               aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATTTTAAGTTTAAAAatta                                                                               >  1:6154573/1‑128 (MQ=255)
                                                 tAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAattatt                                                                             >  1:2746993/1‑128 (MQ=255)
                                                   aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGATTAAAAATTATTCa                                                                           >  1:4681770/1‑128 (MQ=255)
                                                     aTAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaa                                                                         <  1:4442463/128‑1 (MQ=255)
                                                       agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                                       <  1:3487398/128‑1 (MQ=255)
                                                        gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                                      >  2:5268081/1‑128 (MQ=255)
                                                         agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                                     <  1:3622046/128‑1 (MQ=255)
                                                         agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAAACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                                     >  2:2710592/1‑128 (MQ=255)
                                                          gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcc                                                                                                      <  1:2855801/94‑1 (MQ=255)
                                                          gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                                    >  1:1403826/1‑128 (MQ=255)
                                                          gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAAATATAATATTTTATAATATAAAAAGCAATTAAACACACAAAAACTCACCCATAATAATCAGTAAAAAAAATATTCaaaactaa                                                                    >  1:967323/1‑124 (MQ=255)
                                                              aGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                                <  1:852059/128‑1 (MQ=255)
                                                              aGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                                >  1:5375952/1‑128 (MQ=255)
                                                                       tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattat                                                              >  2:489746/1‑121 (MQ=255)
                                                                       tctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAAt                                                       >  1:1619456/1‑128 (MQ=255)
                                                                        ctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                                      <  2:396365/128‑1 (MQ=255)
                                                                         ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                                     >  1:4163457/1‑128 (MQ=255)
                                                                          tAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGTGTTTAAAAATTATTCAAAAATTTGATTATTTAAAATaaa                                                    >  1:404519/1‑128 (MQ=255)
                                                                          tAACAACAAAACTACACACGATTATTTAAATAAAAATTATTTTAATAAAAAAAGCAAAAAAACACACAAAAACTCACACAAAATGCAGAGTTTAAAAATTATTCAAAAATTTTATAATTTTAAATCaa                                                    >  1:7239840/1‑128 (MQ=255)
                                                                           aacaacTAAACTACACTCGATTAATTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCCCCCATTATGGAGAGTTTAAAAATTATTCAAAAATTTGATTAATTAAAATCAAt                                                   >  2:6077160/1‑128 (MQ=255)
                                                                            acaacTAAACTACACTCGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                                  <  2:3694593/128‑1 (MQ=255)
                                                                            acaacTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                                  <  2:6707363/128‑1 (MQ=255)
                                                                             caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTg                                                 >  1:2467779/1‑128 (MQ=255)
                                                                              aactaaactaCAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                                <  2:4374756/128‑1 (MQ=255)
                                                                               actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATTGTGAGTTTAAATATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                               >  2:5414893/1‑128 (MQ=255)
                                                                               actaaactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                               <  2:85752/128‑1 (MQ=255)
                                                                               actaaactaCACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                               <  1:5526027/128‑1 (MQ=255)
                                                                               aataaaataCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                               <  1:2969030/126‑1 (MQ=255)
                                                                                 taaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGc                                             >  2:2315646/1‑128 (MQ=255)
                                                                                  aaactaCACTCGATCATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAGTTGAAGCt                                            <  2:4239807/128‑1 (MQ=255)
                                                                                   aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                                           <  2:2034796/128‑2 (MQ=255)
                                                                                   aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                                           <  1:2339422/128‑1 (MQ=255)
                                                                                     ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                                         <  2:1753662/128‑1 (MQ=255)
                                                                                      taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTcccg                                        >  1:1507874/1‑126 (MQ=255)
                                                                                      taCACTCCAATATTTAATAATATTTTTTTTTAATATAAAAAGCAATACAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtggag                                        <  2:3218281/128‑5 (MQ=255)
                                                                                       acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                       <  2:7129175/128‑1 (MQ=255)
                                                                                         acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTTATTATTTTAAATAAATTGAAGCTCCAGCtt                                     >  1:6404927/1‑128 (MQ=255)
                                                                                          ctggtttattTAATTATTTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAAGCATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                                    <  1:334912/123‑1 (MQ=255)
                                                                                            cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                                  >  2:7238704/1‑128 (MQ=255)
                                                                                            cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                                  >  1:2109939/1‑128 (MQ=255)
                                                                                             gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCCCCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                                 >  2:3058213/1‑128 (MQ=255)
                                                                                             gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                                 <  1:6805179/128‑1 (MQ=255)
                                                                                              attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                                >  2:1490071/1‑128 (MQ=255)
                                                                                                tattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTaa                              <  2:5954358/128‑1 (MQ=255)
                                                                                                 attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                                >  1:4847508/1‑109 (MQ=255)
                                                                                                 attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCATCTAAt                             >  1:5914542/1‑128 (MQ=255)
                                                                                                 attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                             <  2:5716957/128‑1 (MQ=255)
                                                                                                  tttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAtt                            >  2:2569928/1‑128 (MQ=255)
                                                                                                   ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                           <  1:7086508/128‑1 (MQ=255)
                                                                                                     aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg                         <  1:1858870/128‑1 (MQ=255)
                                                                                                     aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTAAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGATTCAACTAATTTAg                         >  1:5092246/1‑128 (MQ=255)
                                                                                                      aTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTCAAGCTCCATCTACAACTAATTTAGc                        >  1:3768386/1‑128 (MQ=255)
                                                                                                       ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCt                       >  2:4760074/1‑128 (MQ=255)
                                                                                                        tataTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCtt                      >  1:6675787/1‑128 (MQ=255)
                                                                                                         atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt                     >  2:2185816/1‑128 (MQ=255)
                                                                                                         atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt                     >  2:5839565/1‑128 (MQ=255)
                                                                                                           atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCATTTGAAGCTACATCTACAACTAATTTAGCTTTCa                   >  1:3250737/1‑128 (MQ=255)
                                                                                                              tttttttaATATAAAAAGAAATTAAACACACAAAAACTGACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt                <  1:4971615/128‑1 (MQ=255)
                                                                                                               ttttttaATATATAAAGCAATAAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttc               <  1:6957303/128‑1 (MQ=255)
                                                                                                                tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct              <  2:5126306/128‑1 (MQ=255)
                                                                                                                 ttttaatataaaaagCAATTAAACAAACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCACTTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttctt             <  2:2943121/119‑1 (MQ=255)
                                                                                                                  tttaatataaaaagCAATTAAACACACAAAAACTCACCCATTTTGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttcttc            >  2:1095726/9‑128 (MQ=255)
                                                                                                                     aatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGc         <  2:3828094/123‑1 (MQ=255)
                                                                                                                      atataaaaagCAATTAAACACACAAAAACTCACCAATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGct        <  1:5659222/124‑1 (MQ=255)
                                                                                                                       tataaaaagCAATTAAACACACAAAAACTCCCCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCGTTCATTTCTTCAGctt       >  2:4629675/4‑128 (MQ=255)
                                                                                                                       tataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGctt       <  2:3574981/125‑1 (MQ=255)
                                                                                                                       tataaaaacCAATTAAACACACAAAAACTCACCCATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGATCCAGCTTCAACTAATTTAGAATTCAATTCTCAAGctt       >  1:431222/4‑128 (MQ=255)
                                                                                                                            aaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTtcttct  <  1:6271811/128‑1 (MQ=255)
                                                                                                                            aaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTtcttct  >  2:3438803/1‑128 (MQ=255)
                                                                                                                               |                                                                                                                            
AAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484674‑484925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: