Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 516,354 A→T intergenic (+185/+321) JCVSYN2_02375 → / ← JCVSYN2_02380 antitermination protein NusG/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816516,3540AT99.8% 9834.8 / ‑4.5 3339intergenic (+185/+321)JCVSYN2_02375/JCVSYN2_02380antitermination protein NusG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (6/1);  new base T (1475/1857);  total (1481/1858)
Fisher's exact test for biased strand distribution p-value = 4.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3340 total aligned reads displayed.

AATAAAAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATT  >  CP016816/516232‑516479
                                                                                                                          |                                                                                                                             
 ataaaAACATATAATATATTTTTAAAAAATATGTTTATATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAATATTTTAATaaaaaa                                                                                                                         <  1:3753495/128‑1 (MQ=255)
  taaaAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAaaaaaa                                                                                                                        <  2:90382/128‑1 (MQ=255)
      aaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAaaaaaa                                                                                                                        >  1:1120099/1‑124 (MQ=255)
      aaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAtt                                                                                                                    <  2:747116/128‑1 (MQ=255)
        tatattttatgtTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGTGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAtttt                                                                                                                  >  1:4015538/8‑128 (MQ=255)
         aTAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTa                                                                                                                 >  2:2844927/1‑128 (MQ=255)
          tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAg                                                                                                                >  2:4141899/1‑128 (MQ=255)
           aaaaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAATATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAAAATTTTAGt                                                                                                               >  2:1697146/1‑128 (MQ=255)
             aaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAt                                                                                                             >  1:7156765/1‑128 (MQ=255)
              atatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAtt                                                                                                            >  2:6029311/1‑128 (MQ=255)
               tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                           <  1:2945876/128‑1 (MQ=255)
                atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                          >  1:2363384/1‑128 (MQ=255)
                 tatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAATATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGc                                                                                                         >  1:4984528/1‑128 (MQ=255)
                 tatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGc                                                                                                         >  2:5812410/1‑128 (MQ=255)
                  atTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCa                                                                                                        >  1:1504408/1‑128 (MQ=255)
                    ttttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGTGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAt                                                                                                      <  2:1939350/128‑1 (MQ=255)
                     tttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAtt                                                                                                     <  2:6394167/128‑1 (MQ=255)
                       tAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtat                                                                                                   >  2:4306728/1‑128 (MQ=255)
                        aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtata                                                                                                  <  1:6683378/128‑1 (MQ=255)
                          aaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaa                                                                                                <  1:6878751/128‑1 (MQ=255)
                             atatGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaaaaa                                                                                             <  2:4536624/128‑1 (MQ=255)
                              tatGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAg                                                                                            <  2:5931957/128‑1 (MQ=255)
                               atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                           <  1:948738/128‑1 (MQ=255)
                                 gTTTTTATAAGATTAGAATAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAATATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAATCATTTTAGTATTTGCAATTATAAAAAAGttt                                                                                         <  2:5264284/128‑1 (MQ=255)
                                    tttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAAAATTTTATTATTTGCAATTATAAAAAAGTTAAtt                                                                                      >  2:2117504/1‑127 (MQ=255)
                                     ttATAAGATTAGAAAAAAATCTTATTTTAAGGTCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGa                                                                                     <  2:3009364/128‑1 (MQ=255)
                                     ttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGa                                                                                     <  1:1005562/128‑1 (MQ=255)
                                     ttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGa                                                                                     >  1:468107/1‑128 (MQ=255)
                                      tataAGATTATAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGat                                                                                    <  2:2629524/128‑1 (MQ=255)
                                      tataAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGat                                                                                    <  1:2067809/128‑1 (MQ=255)
                                       ataAGATTAGTAAAATAGCTTATTTAAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGata                                                                                   <  1:1641239/128‑1 (MQ=255)
                                       ataAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGata                                                                                   <  1:5377829/128‑1 (MQ=255)
                                           gATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAatat                                                                               <  2:2251253/128‑1 (MQ=255)
                                             ttAGAAAAAAAGCTTATTTTAAGGTCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                             <  2:654455/128‑1 (MQ=255)
                                             ttAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                             <  2:373078/128‑1 (MQ=255)
                                             ttAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                             <  2:6285849/128‑1 (MQ=255)
                                               aGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCt                                                                           <  2:4445416/128‑1 (MQ=255)
                                                gAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTa                                                                          >  1:5470557/1‑128 (MQ=255)
                                                gAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTa                                                                          >  1:5261637/1‑128 (MQ=255)
                                                 aaaaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTAt                                                                         <  1:482845/128‑1 (MQ=255)
                                                    aataGCTTATTTTAATTGTTTTTTTAAAGATTATTTAAATTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAATTTTTTGATAATATACATATTaa                                                                      <  1:306352/125‑1 (MQ=255)
                                                    aaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATTATAATATACCTATTaa                                                                      >  2:6910938/1‑128 (MQ=255)
                                                    aaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                      <  2:116360/128‑1 (MQ=255)
                                                       aGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAatat                                                                   <  2:5633483/128‑1 (MQ=255)
                                                        gCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataat                                                                                 >  1:4122359/1‑113 (MQ=255)
                                                          ttATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGa                                                                >  1:2205786/1‑128 (MQ=255)
                                                          ttATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGa                                                                >  1:6406413/1‑128 (MQ=255)
                                                            ttaaaataagCTTTTTTATAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                              >  2:3634716/10‑128 (MQ=255)
                                                             ttttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATa                                                             >  2:1802910/1‑128 (MQ=255)
                                                             ttttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATa                                                             >  1:2501793/1‑128 (MQ=255)
                                                              tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                              >  2:5021147/1‑126 (MQ=255)
                                                              tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                            >  2:271529/1‑128 (MQ=255)
                                                              tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                            >  1:3368127/1‑128 (MQ=255)
                                                              tttAAGGGCTTTTTTAAAGAATATTTAAAATTTAGTTTATATTTTTAATTAAAATTATAATAAAAAAAAATTTTAGTATTTGCAATTATAAAAAATTTTATGATAATATACCTATTAATATAGAAtta                                                            >  2:1301277/1‑126 (MQ=255)
                                                               ttAAGGGCTTTTTTAAATATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTTCAATTATAAAAAATTTAATTATAATATACCTATTAATATAGAATaaa                                                           >  1:786980/1‑128 (MQ=255)
                                                                tAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaac                                                          >  2:2517722/1‑128 (MQ=255)
                                                                tAAGGGCTTTTTTAAAGATTATTAAAAAATTTGTTTATATTTTTAATTAAAATTTTAATAAAAAAACATTATAGTATTTGCAATTATAAAAAAGTTTATCAAAATATAACTATTAATATAGAATAaac                                                          >  1:5879582/1‑128 (MQ=255)
                                                                 aaGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                              >  1:5706153/1‑123 (MQ=255)
                                                                 aaGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaaca                                                         <  1:1343175/128‑1 (MQ=255)
                                                                  aGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaacaa                                                        <  1:4396585/128‑1 (MQ=255)
                                                                       ttttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGa                                                   <  2:7160179/128‑1 (MQ=255)
                                                                        tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                  >  2:3338545/1‑128 (MQ=255)
                                                                         ttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaa                                                 >  1:5578300/1‑128 (MQ=255)
                                                                           ttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAag                                               >  2:3874399/1‑128 (MQ=255)
                                                                            tAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAaga                                              <  1:6765928/128‑1 (MQ=255)
                                                                             aaaGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTATTATAGAATAAACAACTAGAAAagag                                             >  1:51197/1‑128 (MQ=255)
                                                                              aaGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAgaga                                            >  1:3256735/1‑128 (MQ=255)
                                                                              aaGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAgaga                                            >  2:1407303/1‑128 (MQ=255)
                                                                                  ttattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                             >  1:7032792/1‑91 (MQ=255)
                                                                                   tattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAATTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGatatat                                       >  2:7049991/1‑128 (MQ=255)
                                                                                   tattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAatat                                       <  2:6798580/128‑1 (MQ=255)
                                                                                     tttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatta                                     <  2:6165976/128‑1 (MQ=255)
                                                                                     tttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatta                                     >  1:1237619/1‑128 (MQ=255)
                                                                                       tAAAATTTTTTTTTTTTTTTTAATTAAATTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                   <  1:6268387/128‑1 (MQ=255)
                                                                                       tAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                   <  2:3508349/128‑1 (MQ=255)
                                                                                          aaTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGATAAATATTATTAGt                                >  2:4860298/1‑128 (MQ=255)
                                                                                           aTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTa                               >  1:2787701/1‑128 (MQ=255)
                                                                                            ttttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTAt                              <  2:4701159/128‑1 (MQ=255)
                                                                                            ttttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTAt                              <  1:3146060/128‑1 (MQ=255)
                                                                                             tttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATg                             <  2:4005835/128‑1 (MQ=255)
                                                                                              ttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATCTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGa                            >  1:2678980/1‑128 (MQ=255)
                                                                                               tGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGAt                           >  2:5490812/1‑128 (MQ=255)
                                                                                                    ttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATATCGa                      >  1:188054/1‑128 (MQ=255)
                                                                                                    ttatttttaATTAAAATTTTAAAAAAAAAACATTTTAGTATTTTAAATTATAAAAAAGTATATGATAATATAAATATTAATATAGAATAAACAACTACAAAAAACAAATATTATTATTATGATacata                      >  2:1562708/1‑124 (MQ=255)
                                                                                                      atttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaat                    >  1:6141099/1‑128 (MQ=255)
                                                                                                      atttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaat                    >  2:6687729/1‑128 (MQ=255)
                                                                                                          ttaattaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaattaat                <  1:3898268/128‑1 (MQ=255)
                                                                                                            aattaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttt              <  1:4256816/128‑1 (MQ=255)
                                                                                                            aattaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTAAGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttt              <  2:3212268/128‑1 (MQ=255)
                                                                                                              ttaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGGAATATTATTAGTATGATAGCGAATTAAttttt            >  1:6014145/1‑128 (MQ=255)
                                                                                                              ttaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttttt            <  2:3115633/128‑1 (MQ=255)
                                                                                                               taaAATTTTAATAAAAAACCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAATAATTATTTGTATGATAGGGAATTAAtttttt           >  1:3601890/1‑127 (MQ=255)
                                                                                                               taaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTa           >  2:2384255/1‑128 (MQ=255)
                                                                                                                 aaaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaa         >  2:5139948/1‑128 (MQ=255)
                                                                                                                 aaaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTAAGATAATATACCTATTAATATAGAATAAACAACAAGAAAAGAGAAATATTATTAGTATGATAGCAAATTAATTTTTaaa         >  1:4556916/1‑128 (MQ=255)
                                                                                                                     tttAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAataa     >  1:3489146/1‑126 (MQ=255)
                                                                                                                      ttAATAAAAATACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATATACAACTAGAAAAGAGAAATATTATTAGTATGAAAGCGAATTAATTTTTAAAattta    <  1:1757734/128‑1 (MQ=255)
                                                                                                                      ttAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAattta    <  1:6512714/128‑1 (MQ=255)
                                                                                                                      ttAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAattta    >  2:1063342/1‑128 (MQ=255)
                                                                                                                      ttAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAAATAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATATTTAAAatata    >  2:5363696/1‑128 (MQ=255)
                                                                                                                          |                                                                                                                             
AATAAAAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATT  >  CP016816/516232‑516479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: