BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	122,040	C→A	L262F (TTG→TTT)	JCVSYN2_00470 ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	122,040	0	C	A	99.7%	8314.0 / NA	2659	L262F (TTG→TTT)	JCVSYN2_00470	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base C (4/1); major base A (1299/1347); minor base T (3/5); total (1306/1353)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2715 total aligned reads displayed.

ACTAAAATAGCTAAAACTACTGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATCAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTAAAGCAGCTTCTTC  >  CP016816/121915‑122165

aCTAAAATAGCTAAAACTACTGAAAAAGCAGCACCATATCCAAAGTCATTTCCTTTGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATaaa                                                                                                                             <  1:1162710/128‑1 (MQ=255)

  tAAAATAGCTAAAACTACTGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAAc                                                                                                                           >  2:6121301/1‑128 (MQ=255)

   aaaaTAGCTAAAACTACTGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACt                                                                                                                          >  1:1727699/1‑128 (MQ=255)

        aGCTAAAACTACTGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACtagtag                                                                                                                     >  2:6227361/1‑128 (MQ=255)

        aGCTAAAACTACTGAAACAGCAGCACCATATCCAAAGTCATATCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACtagtag                                                                                                                     <  2:5744171/128‑1 (MQ=255)

         gCTAAAACTACTGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTagc                                                                                                                    >  2:2662554/1‑128 (MQ=255)

           tAAAACTACTGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTagcag                                                                                                                  >  2:7140351/1‑128 (MQ=255)

           tAAAACTACTGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTagcag                                                                                                                  <  1:4867372/128‑1 (MQ=255)

           tAAAACTACTGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTagcag                                                                                                                  <  2:5529400/128‑1 (MQ=255)

                  actGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGaac                                                                                                           >  1:83518/1‑128 (MQ=255)

                    tGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAATATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCAGCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGaaata                                                                                                         >  1:5647814/1‑128 (MQ=255)

                     gAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGaactaa                                                                                                        <  2:1478478/128‑1 (MQ=255)

                       aaCAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGaactaaaa                                                                                                      >  2:2756298/1‑128 (MQ=255)

                        aCAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTGGTAGCAGCTTGaactaaaac                                                                                                     <  1:3975811/128‑1 (MQ=255)

                         cagcagCACCATATCCAAAGTCATTACCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGaactaaaact                                                                                                    <  1:1912890/128‑1 (MQ=255)

                            cagcaCCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAactaaaactaaa                                                                                                 <  2:4158547/128‑1 (MQ=255)

                            cagcaCCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAactaaaactaaa                                                                                                 >  1:7183100/1‑128 (MQ=255)

                            cagcaCCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACAAAATAAATTCGTCCTCAACCCATATAAAGAACAACAATAAATCTAGAAGAAGCTTGaactataactata                                                                                                 >  2:2122812/1‑126 (MQ=255)

                                 acATATCCAAAGTAATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTACACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAActaaactaaa                                                                                            <  1:6782211/127‑1 (MQ=255)

                                  cATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTaaaa                                                                                           <  2:3849901/128‑1 (MQ=255)

                                  cATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTACTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGATAGAACAAAAAATAAACTaaaa                                                                                           >  1:4170466/1‑128 (MQ=255)

                                   atatCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAg                                                                                          <  2:2228920/128‑1 (MQ=255)

                                    tatCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGc                                                                                         >  1:100419/1‑128 (MQ=255)

                                    tatCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCATCAACACATATAATGAACAACAAAAAAACTATTAGAAGCTTGAACTAAAACTAAACTAAAAGc                                                                                         >  2:74771/1‑128 (MQ=255)

                                    tatCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCATCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGc                                                                                         <  1:2690642/128‑1 (MQ=255)

                                    tatCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCACCAGAAACTAAATAAAATCGTCCTCCACCCATATATTCAACAACAATAAATCTACTAGCAGCATGAACTAAAACTAAACTAAAAGc                                                                                         >  1:6470791/1‑128 (MQ=255)

                                     atCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAACCTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCa                                                                                        >  1:3304592/1‑128 (MQ=255)

                                     atCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCa                                                                                        <  1:6656553/128‑1 (MQ=255)

                                      tCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGACCAACAAATAAACTAGTAGCAGCTTGAACTACAACTCAACTAAACGCAg                                                                                       >  1:4421826/1‑128 (MQ=255)

                                      tCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTAGAACTAACACTAAACTAAAAGCac                                                                                       >  2:6338461/1‑127 (MQ=255)

                                      tCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCACCTTGAACTAAAACTAAACTAAcagcag                                                                                       >  1:2591306/1‑128 (MQ=255)

                                         aaaGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCGGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTa                                                                                    >  1:4278324/1‑128 (MQ=255)

                                             tCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAacac                                                                                >  2:2019433/1‑128 (MQ=255)

                                                tttCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTcc                                                                             >  1:7052660/1‑128 (MQ=255)

                                                 ttCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCATCTTGAACTAAAACTAAACTAAAAGCAGTTAACCCTCCa                                                                            >  1:3713862/1‑128 (MQ=255)

                                                    cTTTGAAAAAATAAGATTGAATAGCAGCAGTATCTAAATAAATTCTTACTCCAGCCATATAATGAACAACAATAAAACTAGTATCAGCTTGAACTAAAACTAATCTAAAAGCTGTTAACACTCCAGGc                                                                         <  2:4287970/128‑1 (MQ=255)

                                                    cTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGc                                                                         <  2:688933/128‑1 (MQ=255)

                                                    cTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACGCCAGGc                                                                         >  1:1619227/1‑128 (MQ=255)

                                                     ttGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCa                                                                        <  1:6009646/128‑1 (MQ=255)

                                                       ggAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATa                                                                      >  2:1123127/1‑128 (MQ=255)

                                                       ggAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATa                                                                      >  1:5467072/1‑128 (MQ=255)

                                                       ggAAAAAATAAGATTCAATAGCAGAAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATa                                                                      <  2:3980625/128‑1 (MQ=255)

                                                        gAATAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAg                                                                     <  1:3867108/128‑1 (MQ=255)

                                                          aaaaaTAAGTATCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAAGAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAgat                                                                   <  1:1415805/128‑1 (MQ=255)

                                                             aaTAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTATAAGCAGTTAACACTCCAGGCATAgatgat                                                                >  1:6135034/1‑128 (MQ=255)

                                                              aTAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGCACTAAACCTAAACTAAAAGCAGTTACCACTCCAGGCATAgatgagc                                                               >  2:5164975/1‑126 (MQ=255)

                                                              aTAAGATTAAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATc                                                               <  2:4674246/128‑1 (MQ=255)

                                                                 aGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTATACTAAAATCAGTTAACACTCCAGGCATAGATGctctga                                                            >  2:1383489/1‑126 (MQ=255)

                                                                  gATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTaa                                                           <  2:3301109/128‑1 (MQ=255)

                                                                   aTTCAATAGCATGAGAAACTAAATAAATTCGTCCTCCACCAATATAATGATCAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTaaa                                                          <  2:5393888/128‑1 (MQ=255)

                                                                      cAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGGAGCTTGATCTAAAACTAAACTAAAAGCAGTTTACACTCCATGCAGAGATGATCTGaatcta                                                       >  2:864684/1‑124 (MQ=255)

                                                                             agcagAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGCACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCAGAGATGATCTTAAAGTAATAGATc                                                >  2:4807530/1‑128 (MQ=255)

                                                                             agcagAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATc                                                <  1:3430970/128‑1 (MQ=255)

                                                                              gcagAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCa                                               <  2:2884473/128‑1 (MQ=255)

                                                                              gcagAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCGTAAAGGAATAGATCa                                               >  2:5038736/1‑128 (MQ=255)

                                                                               cagAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCaa                                              <  2:4927793/128‑1 (MQ=255)

                                                                               cagAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCaa                                              <  2:1756885/128‑1 (MQ=255)

                                                                                   aaCTAAATAACTTCGTCCTCCACACATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATg                                          <  2:3724058/128‑1 (MQ=255)

                                                                                   aaCTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATg                                          >  1:5896706/1‑128 (MQ=255)

                                                                                   aaCTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATg                                          <  2:1619227/128‑1 (MQ=255)

                                                                                   aaCTAAATAAATTCGTCCTCAACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATg                                          <  2:5283644/128‑1 (MQ=255)

                                                                                     cTAAATAAATTCGTCCTCCACCCATATAATGCACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTACACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGtt                                        >  1:3567077/1‑128 (MQ=255)

                                                                                     cTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGtt                                        >  2:2369472/1‑128 (MQ=255)

                                                                                      taaataaaTTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCATAATGTTc                                       >  2:5826953/1‑128 (MQ=255)

                                                                                       aaataaatTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCt                                      >  1:2475841/1‑128 (MQ=255)

                                                                                         ataaatTCGTCCTCAAACCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTaa                                    <  1:2059850/128‑1 (MQ=255)

                                                                                          taaatTCGTCCTCCACCCATATAATGAACAACAATAAATCTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCTGGCATAGATGCTCTTAAACTAATAGATCAAAATGTTCTaca                                   >  1:4994934/1‑126 (MQ=255)

                                                                                             atTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACCCTCCAGGCAAAGATTAACTTAAAGTACTAGATCTAAATGTTCTAAAtgt                                >  1:4559234/1‑126 (MQ=255)

                                                                                             atTCGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAAttt                                >  2:7027510/1‑128 (MQ=255)

                                                                                                cGTCCTCCACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGa                             <  2:5992125/128‑1 (MQ=255)

                                                                                                  tgctccACCCATATAATGAACAACACTACAACTAGTAGCAGCTTGAACTTAAACTATACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTATTAGATCAAAATGTTCTAAAttntnnnn                           <  1:2173464/126‑5 (MQ=255)

                                                                                                   cctccACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGc                          <  1:5767741/128‑1 (MQ=255)

                                                                                                   cctccACCCATAAAATGAACAACTATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGc                          <  1:2291960/128‑1 (MQ=255)

                                                                                                    ctccACCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCt                         >  2:359004/1‑128 (MQ=255)

                                                                                                        aCCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTa                     >  2:6756953/1‑128 (MQ=255)

                                                                                                        aCCCATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTa                     >  2:3472392/1‑128 (MQ=255)

                                                                                                         cccATATAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCTAAATGTTCTAAATTTTGAAGCTCCTaa                    >  1:6927378/1‑128 (MQ=255)

                                                                                                         cacATATAATGATCAACAATAAAACTAGTTGCAGCTTGAACTAAAACTATACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTATAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTaa                    <  1:6285688/126‑1 (MQ=255)

                                                                                                          cgatatAATGAACAACTATAAAACTAGTATCAGCTTGAACTAACACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTTATAGATCAAAATGTTCTAAATTTTGAAGCTCCTaaa                   <  1:6381731/126‑1 (MQ=255)

                                                                                                            atatCATGAACAACAATAAACCTAGTAGCAGCTTGAACTAAAACTAATCTAAAAGCTGTTAAGACTCCTGGCATAGATGATCTTATAGTAATAGATCAATATGTTCTAAATTTTGATGCTCCTAAATc                 <  1:5124586/128‑1 (MQ=255)

                                                                                                            atatAATGAACAACTATATAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATc                 <  2:2999320/128‑1 (MQ=255)

                                                                                                            atatAATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATc                 <  1:2922766/128‑1 (MQ=255)

                                                                                                             tctactGAACAACTAAAAAACTAGTAGCAGCTTGAAGTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCt                <  1:976427/123‑1 (MQ=255)

                                                                                                              ataATGAACAACAATTAACCTAGTAGCTGCTTGAACTAAAACTAAACTAATAGCAGTTAACACTCCAGGCATAGATGATCTTAATGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTa               <  1:2803543/128‑1 (MQ=255)

                                                                                                              ataATGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTa               <  1:1493463/128‑1 (MQ=255)

                                                                                                                aaTGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTaaa             <  1:469907/128‑1 (MQ=255)

                                                                                                                  tGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTATATTTTGAAGCTCCTAATTCTAAagc           >  2:1245024/1‑128 (MQ=255)

                                                                                                                  tGAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTAAagc           <  2:6560812/128‑1 (MQ=255)

                                                                                                                   gAACAACAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACGAAAAGCAGTTAACACTCCAGGCATAGCTGATCTTAAAGTAATAGAGCAAATTGAGATAAATTTTGAAGCTCGTAACTCTAAagac          >  1:5265212/1‑126 (MQ=255)

                                                                                                                    aacaacAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTAAagcag         <  2:1867121/128‑1 (MQ=255)

                                                                                                                    aacaacAATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTAAagcag         <  2:2522520/128‑1 (MQ=255)

                                                                                                                     acaacaATAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCAGAGATGATCTTAAAGTAAAAGATCAAAATGTTCTAAATTTTGAAGATCCTAAATCTAAaccagc        >  1:737288/1‑128 (MQ=255)

                                                                                                                      caacaaAAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCt                <  2:6462029/119‑1 (MQ=255)

                                                                                                                        acaaTAAATCTAGTAGAAGCTTGAACTAAAACTAAACTAAAAGCAGTATACACTCCTGGAATAGTTGCTCTTAAAGTAATAGATCAAAATGATCTAAATTTTTAATCTCGGAAATCTAACGCATcttc     >  2:4575454/1‑128 (MQ=255)

                                                                                                                        acaaTAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGCTCTGTAAGAAATAGATCAAAAAGTTCTAAATATTGAAGCGCCTAAATTAAAagccccgtc     >  1:3052257/1‑122 (MQ=255)

                                                                                                                        acaaTAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTAAAGCAGcttc     >  2:5628993/1‑128 (MQ=255)

                                                                                                                        acaaTAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTAAAGCAGcttc     >  1:3176701/1‑128 (MQ=255)

                                                                                                                        acaaTAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTAAAGCAGcttc     >  1:1280487/1‑128 (MQ=255)

                                                                                                                          aaTAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTAAAGCAGcttctt   >  2:3172304/1‑128 (MQ=255)

                                                                                                                          aaTAAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATAAAAATGTACTAAATTTTGAATATCCTAAATCAAAAGCAGcttctt   >  2:4442844/1‑128 (MQ=255)

ACTAAAATAGCTAAAACTACTGAAACAGCAGCACCATATCCAAAGTCATTTCCTTGGAAAAAATAAGATTCAATAGCAGCAGAAACTAAATAAATTCGTCCTCCACCCATATAATGAACAACAATCAAACTAGTAGCAGCTTGAACTAAAACTAAACTAAAAGCAGTTAACACTCCAGGCATAGATGATCTTAAAGTAATAGATCAAAATGTTCTAAATTTTGAAGCTCCTAAATCTAAAGCAGCTTCTTC  >  CP016816/121915‑122165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`