Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.5% 7039.1 / 5.0 2437intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (12/49);  new base T (1382/994);  total (1394/1043)
Fisher's exact test for biased strand distribution p-value = 1.64e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2521 total aligned reads displayed.

TATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165091‑165347
                                                                                                                                |                                                                                                                                   
  tttACTTTAATTGTATTACTTTACTAGTTTATAATATTTAGTTTCATTAATTTATCGAACTTTTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                   <  2:5398382/128‑1 (MQ=255)
     aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                                >  2:6859853/1‑126 (MQ=255)
       tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTAAAATTTTTAAAAaat                                                                                                                              >  1:5969351/1‑128 (MQ=255)
       tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                              >  2:1131678/1‑128 (MQ=255)
         tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAATTTTTTAAAAaatta                                                                                                                            >  1:3628981/1‑128 (MQ=255)
         tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                            >  2:6121375/1‑128 (MQ=255)
         tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                            >  1:3369244/1‑128 (MQ=255)
          aaTTGTATTTCTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                           <  2:4489590/128‑1 (MQ=255)
           aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                          <  1:5731090/128‑1 (MQ=255)
              gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                       >  2:56765/1‑128 (MQ=255)
                aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATAAGCAAATTTATAAAAAATTAATTTGaa                                                                                                                     >  2:1710988/1‑128 (MQ=255)
                aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATAGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                     >  1:4638231/1‑128 (MQ=255)
                 ttactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAc                                                                                                                    >  1:5263241/1‑128 (MQ=255)
                  tactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACt                                                                                                                   >  1:4355381/1‑128 (MQ=255)
                   actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                                  <  1:4735178/128‑1 (MQ=255)
                    ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                                 <  1:1007831/128‑1 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                      >  1:6791332/1‑122 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                >  2:5773900/1‑128 (MQ=255)
                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATAAATTTGAACTTGt                                                                                                                <  1:585112/128‑1 (MQ=255)
                     tttactAGTTGATAATATTAAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTAGATGCAAATTTTTAAAAAATTAATTTTAACTAGt                                                                                                                >  1:3788972/1‑128 (MQ=255)
                      ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                               >  2:506169/1‑128 (MQ=255)
                        actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                             <  1:5571780/128‑1 (MQ=255)
                           aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                          <  2:7186003/128‑1 (MQ=255)
                             ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                        >  2:1263059/1‑128 (MQ=255)
                             ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                        >  1:3110630/1‑128 (MQ=255)
                                  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATATCttt                                                                                                   >  1:295043/1‑128 (MQ=255)
                                  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                   >  1:2704558/1‑128 (MQ=255)
                                       ttATTTTCATTAAATTATAGCACTTGTATTAAATATTAACAAACTAAAACAATTATCTTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGCtttttttt                                                                                              <  1:1993899/128‑1 (MQ=255)
                                       ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttt                                                                                              >  2:5323033/1‑128 (MQ=255)
                                          gtttattttaTTTATTGCAATTTTTTGAATTATTAATAAAATAAAACTATTATCTTTTTTTTTATTTTTTGTATATTCAAATTTTTAAAAAATTTATTTTAACTTGTAAAAAAAAGCttttttttttt                                                                                           <  1:6651431/119‑1 (MQ=255)
                                          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                           >  2:6611671/1‑128 (MQ=255)
                                             tCATTAATTTATCGCACATGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATATTTTGTATATGCAAATTATTAAAAAATTAATTTGAACTTGTAAAAAATAGCtatttttttttatt                                                                                        >  1:4232841/1‑128 (MQ=255)
                                                  aaTTTATCGCAATTGTATGTATTAGTAATAAAATAAAAATATTATATTTTTATATATTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGCttttttttttttttttata                                                                                   <  1:1879687/128‑4 (MQ=255)
                                                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGATCTTGTAAAAAATAGCtttttttttttttttattatg                                                                                 >  2:2823081/1‑125 (MQ=255)
                                                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatt                                                                                 >  2:5549172/1‑122 (MQ=255)
                                                     ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttataa                                                                                >  2:421333/1‑124 (MQ=255)
                                                      tataGCAATTGTATTAATTAGTAATAAACTAAAACTATTATCTTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAAATAATTTGAACTTTTAAAAAATAGCttttttttttttttttataatgg                                                                               <  2:3386566/128‑8 (MQ=255)
                                                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatttaaat                                                                            >  2:3295742/1‑117 (MQ=255)
                                                          gCACTTGTATTAATTTTTAATAAAATAAAAATTTTATCTTTTTATTTATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGTACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAat                                                                        <  1:2384756/128‑1 (MQ=255)
                                                          gCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATATCttttttttttttttttataatttaaat                                                                           >  2:5193564/1‑117 (MQ=255)
                                                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTatattttaaataa                                                                        >  1:2827036/1‑119 (MQ=255)
                                                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGAAAAtata                                                                     >  1:6228348/1‑128 (MQ=255)
                                                              ttttATGAATTAGTAATAAACTAAAAATAATATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                   <  2:2946067/125‑1 (MQ=255)
                                                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTATTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTatatttaaaatata                                                                       >  2:6027790/1‑118 (MQ=255)
                                                                 ttttattatttAAAAAAATAAAATTTTATCTTTTTATATATTTTTTTTATAAGCAAATTTTTAAAAAAATAAATTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:6229797/118‑1 (MQ=255)
                                                                 tttGAATTAGTAACTAACTAATAATATTATATTTTTAGAAATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:6747361/126‑1 (MQ=255)
                                                                 tatttatttttaataaAATAAAAATATTATATTTTTTGATATTTTTTGTTTATTTAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  1:7058739/118‑1 (MQ=255)
                                                                 tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATaa                                                                    <  2:2318100/124‑1 (MQ=255)
                                                                  ttttaatattAACAAACAAAAACAATTTTTTTTTTTTATATTTTTTTTATATTCAAATTTTTAAAAAAATAATTTTAAATTTTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  1:6924228/119‑1 (MQ=255)
                                                                  tttattttttaataaAATAAAAATATTAAATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:6522853/119‑1 (MQ=255)
                                                                  ttGAATTATTTATAAAAAAAAAATATTATTTTTTTATATATTTTTTTTATTTTAAAATTTTTAAAAAATTAATTTTAATTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:4062031/127‑1 (MQ=255)
                                                                    gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAATTTTTa                                                              >  1:4081115/1‑128 (MQ=255)
                                                                      attatttattaactaaaactaTTATCTTTTTAGATATTTTTTTTTTAAGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAAAA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                           <  1:7166264/118‑1 (MQ=255)
                                                                      attattAATAAAATAAAAATATTTTATTTTTATATATTTTTTGTTTATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                           <  1:2105154/128‑1 (MQ=255)
                                                                       attttaataaAATAAAATTATTATTTTTTTTTTATTTTTTTTTTTTTTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAA‑‑TACTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                          <  1:920696/124‑1 (MQ=255)
                                                                        tattAAGAAAATAAAAATATTATTTTTTTTGTTATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                         <  2:2624331/125‑1 (MQ=255)
                                                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAgtaa                                                         >  2:1418682/1‑125 (MQ=255)
                                                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtt                                                      >  1:393402/1‑128 (MQ=255)
                                                                           taataaACTTAAACTATTATCTTTTTTGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtttt                                                    <  2:3766310/128‑1 (MQ=255)
                                                                             aGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTAtttt                                                    >  1:6332841/1‑128 (MQ=255)
                                                                              gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTATGTATTttgt                                                  >  2:6363311/1‑128 (MQ=255)
                                                                               aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAATTATAAATTTTAGTTTTTttgtt                                                 >  1:3495299/1‑128 (MQ=255)
                                                                                    aaaaataATATATTTTTAGTTATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                            <  2:1554473/128‑1 (MQ=255)
                                                                                     aaaataTTATATTTTTATTTATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGTAAAAAAAAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                          <  2:4624788/128‑1 (MQ=255)
                                                                                         tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAATTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTAATTTTGTTGTCTAAGTAt                                       >  1:2570605/1‑128 (MQ=255)
                                                                                          tttttttttttATTTATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑‑‑TTGGTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                     <  1:5398881/122‑1 (MQ=255)
                                                                                          attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATtttttttaataattata                                      >  2:3168273/1‑117 (MQ=255)
                                                                                           ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTGAAATAAAAATTTTATTTATTTTTTTTTATAATtatata                                    >  2:6959392/1‑128 (MQ=255)
                                                                                            tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATAAT‑‑TGGAAATAAAAAATTTATTTATTTTTTTTTATaattttat                                     >  2:785310/1‑122 (MQ=255)
                                                                                               cTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTGTTGTATAATTaaaaaaaa                                 >  1:1610840/1‑121 (MQ=255)
                                                                                                 ttttATATATTTTTTTTATAATCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaa                               <  2:901171/128‑1 (MQ=255)
                                                                                                 ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTAGGTATTTTTATATATAAGTAAATaaaaa                                >  2:3920473/1‑128 (MQ=255)
                                                                                                   ttATATATTTTTTGTTTATTTAAATTTTTAAAAAATTAATTTTAAAATTTTAAAAATA‑‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAAt                             <  2:2063795/128‑1 (MQ=255)
                                                                                                     ttattttttttttttATTTAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                           <  2:3072311/123‑1 (MQ=255)
                                                                                                     atatatTTTTTGTATTAGCAAATTTTTTAAATATATAATTTAACTTTTAAAAAATAGC‑‑‑CTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                           <  1:1492816/126‑1 (MQ=255)
                                                                                                       attttttttttATATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                          <  2:4357918/125‑1 (MQ=255)
                                                                                                       atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAATaaaa                         >  2:3511839/1‑128 (MQ=255)
                                                                                                         atTTTTTTTATATTAAATTTTTTAAAAAATTTATTTTTAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                       <  2:1927753/128‑1 (MQ=255)
                                                                                                           ttttttTATATTGAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑TGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                     <  2:2162571/128‑1 (MQ=255)
                                                                                                           tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑‑‑ATGAAATTATAAATTTTAGGTATTTTGTTTTATAATTATATAAAAAAATAGAAAgta                     >  1:784038/1‑126 (MQ=255)
                                                                                                            ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAAATAAAAAAAAAATTTTAGTTATTTATATTTAAAAGTAAATAAAAAAATATaaaat                      >  1:2227754/1‑126 (MQ=255)
                                                                                                            ttatttctatttaaaTTTTTAAAAAATTTATTTTAAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                    <  1:6501461/116‑1 (MQ=255)
                                                                                                             tttttttatttaaaTTTTTAAAAAATTTATTTTTAATTGTAAAAAAATTCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                    <  2:1855165/117‑1 (MQ=255)
                                                                                                              tttaattttgaaaTTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                   <  2:4721411/118‑1 (MQ=255)
                                                                                                              ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATTTAAATATAAATTTTATGTATTTTTTTTTATAATTAAATAAAAAAATATAAATGaa                    >  2:3661711/1‑128 (MQ=255)
                                                                                                               tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTGTATAATTTTATAAAAAAATTGAAATGaaaaa                 >  2:7110292/1‑128 (MQ=255)
                                                                                                                gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTTTTTTTTTTTCTAAGTTTCTAAAAAAATATAAATGaaaaaa                >  2:2468156/1‑128 (MQ=255)
                                                                                                                gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAATTATATAAAAAAATATAAATTAAAAAat               >  2:5923125/1‑128 (MQ=255)
                                                                                                                 tataTGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGAAAATAAAAATTTTAGGTATTTTTTTTTATAAGTATATAAAAAAATATAAAGTaaaaaa                >  1:3230499/1‑128 (MQ=255)
                                                                                                                  atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTTATTTTTTTTTATATTTTTATAAAAAAATATAAATTaaaaaa                >  2:2727507/1‑128 (MQ=255)
                                                                                                                   tatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTATTTTTT‑‑‑‑TAATGGAAATTAAAATTTTAGTTTTTATTTTGAATAAATAGATAATAAATTAAAAAGCaaaaaaattt            >  1:5028869/1‑124 (MQ=255)
                                                                                                                   tatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTTTTGTATAATTATATAAAAAAATAAAAATTAAAAAatat             >  1:4475852/1‑128 (MQ=255)
                                                                                                                     tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAAATAGAAATGaaaaaaattta           >  2:4955697/1‑123 (MQ=255)
                                                                                                                        aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATTT‑‑TTTAAATATAAATTTTAGGTTTTTTTTTGTATAATTATAAAAAAAAATAAAAAGGAAAAAATATTTTTa        >  1:1149362/1‑128 (MQ=255)
                                                                                                                        aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTGTAAATATAAATTTTAGTTATTTTTTTTTATAAGTATATAAAAAAATAAAAAGTAAAAAATAttttaa        >  1:1335864/1‑124 (MQ=255)
                                                                                                                        aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAAAGTAAATAAAAATTTATGGTATTTTGTTTTGTAATTATATAAAAAAATAAAAATTAAAAAATATTGTTaa       >  1:5133299/1‑128 (MQ=255)
                                                                                                                         aaTTTTTAAAAAATTAATTTTAACATGTTAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAGGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa       <  1:1741380/128‑1 (MQ=255)
                                                                                                                          aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTAAAATTAAATTTTATGGTATTTTTTTTGTATTGTTATAAAAAAAAATAAAAGGAAAAAATATTTTTaa       >  1:485404/1‑128 (MQ=255)
                                                                                                                          aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAAATAAAATTTTATTTATTTTTTTTTATAATTATATAAAAAATTATAAATAAAAAAAAATTTTTaaa      >  2:4810552/1‑128 (MQ=255)
                                                                                                                            ttttAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTCTTTTTTTTTTTTTTAATGTAAATATAAATTTTAGTTATTTTTTTTTATAATTAAATAAAAAAATAAAAAGAAAAAAAAATTTTTaa       >  1:6087821/1‑128 (MQ=255)
                                                                                                                                |                                                                                                                                   
TATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165091‑165347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: