BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	281,616	C→T	Q100* (CAA→TAA)	JCVSYN2_01185 →	DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	281,616	0	C	T	99.8%	10132.6 / NA	3148	Q100* (CAA→TAA)	JCVSYN2_01185	DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (1528/1611); minor base A (5/1); total (1536/1612)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3179 total aligned reads displayed.

ATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281491‑281740

aTGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATaa                                                                                                                            >  1:1665334/1‑128 (MQ=255)

aTGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATaa                                                                                                                            <  1:6628953/128‑1 (MQ=255)

 tGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAaataat                                                                                                                           >  1:4226599/1‑128 (MQ=255)

  gATTCTATTTCTAAAACTATTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAtt                                                                                                                          <  2:3498429/128‑1 (MQ=255)

      cTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTtata                                                                                                                      <  1:4108228/128‑1 (MQ=255)

        aTTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaaa                                                                                                                    >  1:798999/1‑128 (MQ=255)

           tCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAg                                                                                                                 >  2:2237524/1‑128 (MQ=255)

           tCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAg                                                                                                                 >  2:731393/1‑128 (MQ=255)

           tCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAg                                                                                                                 >  1:4966100/1‑128 (MQ=255)

            cTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGt                                                                                                                <  2:6730958/128‑1 (MQ=255)

             tAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAATAATAATTTATAAAAAGtt                                                                                                               >  2:5646383/1‑128 (MQ=255)

             tAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGtt                                                                                                               <  1:3671607/128‑1 (MQ=255)

                aaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTATCTAATTAATAAAATTGAAACAAATCAAATATATGAATAATAATTTATAAAAAGTTaaa                                                                                                            >  1:2607899/1‑128 (MQ=255)

                 aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                           <  2:4529580/128‑1 (MQ=255)

                  cTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaaa                                                                                                          >  1:5245962/1‑128 (MQ=255)

                   tAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAag                                                                                                         <  1:4593545/128‑1 (MQ=255)

                     gTAAATTATTATTTAATTTAGAGACAAAATTAAATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                       <  2:7140723/128‑1 (MQ=255)

                      tAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATTGATGAAAAATAATTTATAAAAAGTTTAAAAGAgg                                                                                                      <  1:2862497/128‑1 (MQ=255)

                       aaaTTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGt                                                                                                     >  1:3111044/1‑128 (MQ=255)

                         attattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGTATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGa                                                                                                   >  1:1567917/1‑128 (MQ=255)

                         attattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGa                                                                                                   >  1:5958211/1‑128 (MQ=255)

                         attattattTAATTTAGAGACAAAATTACAATTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGa                                                                                                   <  1:4463755/128‑1 (MQ=255)

                          ttattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAt                                                                                                  <  1:470608/128‑1 (MQ=255)

                          ttattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATAAAATATATTAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAt                                                                                                  >  1:6506537/1‑128 (MQ=255)

                           tattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAaga                                                                                                        <  2:1514556/121‑1 (MQ=255)

                            attattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAttt                                                                                                >  1:5677013/1‑128 (MQ=255)

                             ttattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtg                                                                                               <  1:2755813/128‑1 (MQ=255)

                              tattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGGGATTtga                                                                                              <  1:6184802/128‑1 (MQ=255)

                               atttaatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtgat                                                                                             >  2:4941581/1‑128 (MQ=255)

                                 ttaatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtgatga                                                                                           >  2:2076579/1‑128 (MQ=255)

                                 ttaatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtgatga                                                                                           <  2:3405230/128‑1 (MQ=255)

                                  taatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGaa                                                                                          <  2:5946754/128‑1 (MQ=255)

                                   aatttaGAGACAAAATTACATTTAAAACAAAATCAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGaaa                                                                                         <  1:3241470/128‑1 (MQ=255)

                                    atttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGATGATTTGATGAAAg                                                                                        >  2:5790977/1‑128 (MQ=255)

                                     tttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGt                                                                                       <  1:1444844/128‑1 (MQ=255)

                                       ttgtctctaaaTTACATTTAAAACAAAAACAGGATTAACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGTAGATTAGATGAAAGTAt                                                                                     >  1:7210723/9‑128 (MQ=255)

                                       taGATACAAAATTAAATTTAAAACAAAAACAGTATTTAATAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTAt                                                                                     <  1:4844248/128‑1 (MQ=255)

                                           gaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAAt                                                                                 <  1:1002390/128‑1 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGt                                                                                                                >  2:4052331/1‑94 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGaa                                                                                          <  1:2219393/116‑1 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAt                                                                              >  1:2978540/1‑128 (MQ=255)

                                              aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGAt                                                                              >  2:478857/1‑128 (MQ=255)

                                               aaaTTACATTTAAAACAATATCAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATc                                                                             <  2:2542665/128‑1 (MQ=255)

                                                       tttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGt                                                                     <  1:1272648/128‑1 (MQ=255)

                                                       tttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTATAAAAGAGGTGATTTGATGAAAGTATTAATGATCTGAGATGt                                                                     >  2:5380956/1‑128 (MQ=255)

                                                        ttAAAACAAAAACAGTATTTACTAATTTATCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGtt                                                                    <  2:2374544/128‑1 (MQ=255)

                                                        ttAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGtt                                                                    <  2:5482293/128‑1 (MQ=255)

                                                         tAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGttt                                                                   <  2:506536/128‑1 (MQ=255)

                                                         tAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGttt                                                                   >  2:1357049/1‑128 (MQ=255)

                                                          aaaacaaaaactGGATTTACTTATTTATCTAATTAATAATATTGAAACATATCAAATAGATGATAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  <  2:1223912/128‑1 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  >  2:315319/1‑128 (MQ=255)

                                                          aaaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  >  2:6999269/1‑128 (MQ=255)

                                                            aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATg                                                                >  2:1665827/1‑128 (MQ=255)

                                                            aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATATGATGAAAGTATTAATGATCGGAGATGTTTATg                                                                >  2:3804433/1‑128 (MQ=255)

                                                             acaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGc                                                               <  2:619774/128‑1 (MQ=255)

                                                                aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCaaa                                                            <  1:707307/128‑1 (MQ=255)

                                                                 aaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCaaaa                                                           <  2:843802/128‑1 (MQ=255)

                                                                     aGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGATGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAAcctg                                                       >  2:1090888/1‑126 (MQ=255)

                                                                      ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATg                                                                >  2:1908540/1‑118 (MQ=255)

                                                                      ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCggg                                                      >  1:2330346/1‑128 (MQ=255)

                                                                      ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCggg                                                      >  1:3548935/1‑128 (MQ=255)

                                                                       gATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGa                                                     >  1:5364555/1‑128 (MQ=255)

                                                                           tACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAACCCGGGAagag                                                 >  2:5190015/1‑128 (MQ=255)

                                                                           tACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGa                                                     >  1:2065444/1‑124 (MQ=255)

                                                                            aCTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAgaga                                                >  1:6281259/1‑128 (MQ=255)

                                                                              tAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGaaa                                              <  2:1799186/128‑1 (MQ=255)

                                                                                aTTTCTCTAATTAATAAAATTGAAAAAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGATGTGATATGATGAAAGTATTAATGATCGGAGATGTTTATGAAAAACCGGGAAGAGAAAta                                            >  2:3630400/1‑127 (MQ=255)

                                                                                     tctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGa                                       >  1:3799469/1‑128 (MQ=255)

                                                                                       taattaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaa                                     <  1:3390308/128‑1 (MQ=255)

                                                                                          ttaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAAc                                  >  1:6376010/1‑128 (MQ=255)

                                                                                            aataaAATTGAAACAACTCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAt                                <  1:5490102/128‑1 (MQ=255)

                                                                                             atataaattaaaaaaATCAAATAGATGAAAAATAATTTTTAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGTGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:4338074/119‑1 (MQ=255)

                                                                                             ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:6926294/128‑1 (MQ=255)

                                                                                             ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:6056718/128‑1 (MQ=255)

                                                                                             ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:920854/128‑1 (MQ=255)

                                                                                             ataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:2474625/128‑1 (MQ=255)

                                                                                              taaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAtt                               <  1:395691/127‑1 (MQ=255)

                                                                                                aaaTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAt                                <  1:1962431/124‑1 (MQ=255)

                                                                                                aaaTTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaa                            >  2:6615058/1‑128 (MQ=255)

                                                                                                   ttGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCTGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaaaaa                         >  2:2784519/1‑128 (MQ=255)

                                                                                                     gAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAat                       <  2:6281259/128‑1 (MQ=255)

                                                                                                      aaaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGaa                                                    >  2:3942665/1‑98 (MQ=255)

                                                                                                       aaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAatat                     <  2:6500846/128‑1 (MQ=255)

                                                                                                       aaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAatat                     <  2:4158771/128‑1 (MQ=255)

                                                                                                        actaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAtt                    <  2:5279108/125‑1 (MQ=255)

                                                                                                        aCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAtt                    <  2:4283534/128‑1 (MQ=255)

                                                                                                         caaatcaaatAGATGAAAAATAATCTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttg                   <  2:3047818/128‑1 (MQ=255)

                                                                                                          aaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgt                  <  2:4398880/128‑1 (MQ=255)

                                                                                                          aaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgt                  >  2:3296498/1‑128 (MQ=255)

                                                                                                            atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgttg                <  2:2933793/128‑1 (MQ=255)

                                                                                                            atcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgttg                >  1:5851808/1‑128 (MQ=255)

                                                                                                             tcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAATAAATATTGTTGa               >  2:4632352/1‑128 (MQ=255)

                                                                                                                   aGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaa         <  2:6376010/128‑1 (MQ=255)

                                                                                                                   aGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGGTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaa         <  2:6867724/128‑1 (MQ=255)

                                                                                                                     aTGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatc       <  2:6075959/128‑1 (MQ=255)

                                                                                                                       gAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGat              >  1:1828813/1‑119 (MQ=255)

                                                                                                                         aaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTa                                                                  >  2:2656027/1‑65 (MQ=255)

                                                                                                                         aaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAAt   >  1:1144261/1‑128 (MQ=255)

                                                                                                                          aaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATa  <  1:5098467/128‑1 (MQ=255)

                                                                                                                          aaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATa  >  1:6808465/1‑128 (MQ=255)

ATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATTAATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281491‑281740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`