Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 20,023 2 bp→TA intergenic (‑744/‑392) JCVSYN2_00065 ← / → JCVSYN2_00070 hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681620,0230AT99.9% 8234.8 / NA 2801intergenic (‑744/‑393)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base T (1470/1326);  total (1473/1328)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP01681620,0240TA99.7% 8558.0 / NA 2801intergenic (‑745/‑392)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base T (4/5);  new base A (1468/1323);  total (1473/1328)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2835 total aligned reads displayed.

TGAGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAGAT  >  CP016816/19896‑20147
                                                                                                                               ||                                                                                                                           
     gCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTta                                                                                                                         >  2:2140602/1‑128 (MQ=255)
      cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                        >  1:2276409/1‑128 (MQ=255)
        ggAAGAGCAAGATAAAAGGTAGTATGTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAatttatatatttatat                                                                                                                      <  1:1152562/128‑1 (MQ=255)
         gAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGCGTCTTTTACATCTTCGGATACCAAAAACTATTTTTTCTTGAATTTCTTGTTGTACTTTCTATTTTTAATTAAtatatttatatt                                                                                                                     >  1:3566496/1‑128 (MQ=255)
          tagagCAAGATAAAAGGTAGTATTTGTTGGCGATTCAGCTAGAGTCTTTTAGATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTa                                                                                                                    <  1:7177251/127‑1 (MQ=255)
             agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                                 >  2:4388478/1‑128 (MQ=255)
             agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                                 >  2:1280441/1‑128 (MQ=255)
             agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTCTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                                 >  1:1646607/1‑128 (MQ=255)
             agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTATTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                                 >  1:2413146/1‑128 (MQ=255)
              gCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaaa                                                                                                                >  2:2262306/1‑128 (MQ=255)
               cAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTtaaaaa                                                                                                               >  1:1002229/1‑128 (MQ=255)
               cAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaaaa                                                                                                               <  1:574299/128‑1 (MQ=255)
                  gATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAttt                                                                                                            >  2:1414041/1‑128 (MQ=255)
                     aaaaTGTAGTATTTGTTTGAGATCCACATAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaaa                                                                                                         <  2:2782310/128‑1 (MQ=255)
                      aaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                        <  1:6213269/128‑1 (MQ=255)
                      aaaGGTAGTATTTGTTGGCGATCCACCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                        <  2:6897503/128‑1 (MQ=255)
                       aaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatt                                                                                                       >  1:4123227/1‑128 (MQ=255)
                       aaGGTAGTATTTGTTGGCGAACCCCCTAGAGTCTATTAAATCTACGGAAAACAAAAACTAATTTTTCTTTAATTTGTTTTTTTTCTTTCTATATTTAATTTATATATTAATATTAAAAaaattaaatt                                                                                                       >  2:6721284/1‑128 (MQ=255)
                        cggTAGTATTTGTTCGCGATCCTCCTAGAGTCTTTTACTTCTTCGGTAAATAAAATCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatta                                                                                                      <  1:2876133/127‑1 (MQ=255)
                           tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAAtt                                                                                                             <  1:3457896/118‑1 (MQ=255)
                           tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                   >  1:5612408/1‑128 (MQ=255)
                           tagaagttgttgGCGATCCCCCTAGAGTCTTATACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                   <  1:3148276/122‑1 (MQ=255)
                            agtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataat                                                                                                  >  2:859976/1‑128 (MQ=255)
                             gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAACACTAATTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATATATATATTTATATTAAAAAATTTAaattataatt                                                                                                 >  1:3259745/1‑128 (MQ=255)
                             gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                                 >  2:4092266/1‑128 (MQ=255)
                                tttGTTGGCGATAACCATAGAGTCTTTTACATCTTCGTAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAattatt                                                                                              <  2:4227896/128‑1 (MQ=255)
                                ttagttgGCGATACCCCTAGAGTCTTTTACATCTTAGGAATACATAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAattatt                                                                                              <  2:5211989/125‑1 (MQ=255)
                                   gtttGCGATCCCCCTATATTCTTTTAAATTTTATGAAAACAAAAACTATTTTTTTATTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttttt                                                                                           <  2:7177397/128‑1 (MQ=255)
                                    ttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTta                                                                                          <  2:3205071/128‑1 (MQ=255)
                                    ttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTta                                                                                          >  1:4007096/1‑128 (MQ=255)
                                     tgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTtat                                                                                         <  2:2392371/128‑1 (MQ=255)
                                      ggCGATCCCCCTAGAGTCTTTTACATCTTCAGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTtata                                                                                        <  1:1924157/128‑1 (MQ=255)
                                        cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                      >  1:4405465/1‑128 (MQ=255)
                                           tCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACg                                                                                   <  2:3558056/128‑1 (MQ=255)
                                           tCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAAATATTTTTATAGAAct                                                                                   >  1:5500159/1‑127 (MQ=255)
                                             ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                 <  1:3802080/128‑1 (MQ=255)
                                              cccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGa                                                                                <  2:2040448/128‑1 (MQ=255)
                                               ccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                          >  2:5085058/1‑101 (MQ=255)
                                                 tAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTAATGCTCGTGATaa                                                                             >  2:4587875/1‑128 (MQ=255)
                                                  tgagTATTTTACATCTTCGTAAAACAAATACTATTTTTTCTTTAATTTATTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAt                                                                            <  1:2541815/127‑1 (MQ=255)
                                                  agagTCTTTTACATCTTAGGAAATCATAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAATAATTTAAATTATAATTATTTTTAAAGCACGTGATAAt                                                                            <  2:3031223/128‑1 (MQ=255)
                                                   gagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAtt                                                                           <  2:2648706/128‑1 (MQ=255)
                                                   gagTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAtt                                                                           <  1:756766/128‑1 (MQ=255)
                                                     gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                         >  2:5672230/1‑128 (MQ=255)
                                                     gTCATTTACATATTCTGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                         <  1:5261837/128‑1 (MQ=255)
                                                       cTTTTCCATCCTCGTAAAACAAAAACAATTTTTTATTTAATTTCTTTTTTTACCTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                       <  1:6741530/128‑1 (MQ=255)
                                                          ttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                       <  2:5530815/125‑1 (MQ=255)
                                                          ttACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGtt                                                                    <  2:6221999/128‑1 (MQ=255)
                                                          atACATCTTCGGAAACCAAAAACTATTTTATCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGtt                                                                    <  1:5017680/127‑1 (MQ=255)
                                                             cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGt                                                                 >  1:921929/1‑128 (MQ=255)
                                                              aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTg                                                                >  1:1542387/1‑128 (MQ=255)
                                                              aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTg                                                                >  1:2042233/1‑128 (MQ=255)
                                                              aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGAGATAATTAAAAGTTAGTg                                                                >  1:3706600/1‑128 (MQ=255)
                                                               tCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGa                                                               >  1:6870792/1‑128 (MQ=255)
                                                                 ttCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAAc                                                             >  1:3384145/1‑128 (MQ=255)
                                                                  acGGAAAACATACAATATTATATCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTAATATATTTATATTAAAACATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACa                                                            <  1:6340690/127‑1 (MQ=255)
                                                                   cGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACaa                                                           <  1:6473878/128‑1 (MQ=255)
                                                                     gAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGa                                                         <  2:4919627/128‑1 (MQ=255)
                                                                         aCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAAc                                                     >  1:4278450/1‑128 (MQ=255)
                                                                          cAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACa                                                    >  1:1763311/1‑128 (MQ=255)
                                                                          cAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACa                                                    >  1:5894304/1‑128 (MQ=255)
                                                                           aaaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACat                                                   >  2:6517343/1‑127 (MQ=255)
                                                                            aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                  >  1:6126824/1‑128 (MQ=255)
                                                                            aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                  <  1:1403103/128‑1 (MQ=255)
                                                                             aaaCTATTTTTTCTGTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTg                                                 <  1:4732010/128‑1 (MQ=255)
                                                                              aaatATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGa                                                <  2:76601/125‑1 (MQ=255)
                                                                               aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGaa                                               >  1:7005489/1‑128 (MQ=255)
                                                                               aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGaa                                               >  2:3446363/1‑128 (MQ=255)
                                                                               aCTATTTTTTCTTTAATTACTTTTTTTACTTTCTATTTTTAAATTATATATTTATATTAAAAAATTTAAATTATAATTATTTATATAGCACCTGATAATTAAAAGTTAGTGAACAAGAAAACAGAGaa                                               >  1:2169478/1‑128 (MQ=255)
                                                                                cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                              <  2:7008509/128‑1 (MQ=255)
                                                                                cTATATTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                              <  2:400420/128‑1 (MQ=255)
                                                                                 tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                             <  1:6628919/128‑1 (MQ=255)
                                                                                 tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                             >  1:5409434/1‑128 (MQ=255)
                                                                                 tATTTTTTCTTTAATTTCTTTTATTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                             <  1:2737576/128‑1 (MQ=255)
                                                                                   ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATATATATATTTATATTAATAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTATAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                           >  2:1884642/1‑128 (MQ=255)
                                                                                    tttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAcc                                          >  2:3849244/1‑128 (MQ=255)
                                                                                      tttCTTTTATTTCTTTTTTTAGTTTCTATTTTTAATTTATTTATTTATATTAAAAAATTTAATTTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAg                                        <  1:5132948/128‑1 (MQ=255)
                                                                                        tCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                             <  2:5938944/121‑1 (MQ=255)
                                                                                         cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTTATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                     >  2:5376302/1‑128 (MQ=255)
                                                                                           ttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCt                                   <  1:4885428/128‑1 (MQ=255)
                                                                                               tttCTTTTTTTACTTTCTATTTTTAATATATATATTTATATTAAAAAATTTAAATTATAATAATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAc                               <  1:3019734/128‑1 (MQ=255)
                                                                                                   tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                           >  2:3300362/1‑128 (MQ=255)
                                                                                                   tttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaa                           >  2:1096440/1‑128 (MQ=255)
                                                                                                    ttttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCaaaa                          >  1:4513936/1‑128 (MQ=255)
                                                                                                       tttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaa                       <  2:4486038/128‑1 (MQ=255)
                                                                                                       tttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaagaa                       <  2:2523828/128‑1 (MQ=255)
                                                                                                        ttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGaa                                               <  2:4779560/103‑1 (MQ=255)
                                                                                                         tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                                 >  2:3739864/1‑68 (MQ=255)
                                                                                                         tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAagaaga                     >  2:1607070/1‑128 (MQ=255)
                                                                                                         tACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAagaaga                     >  1:4617097/1‑128 (MQ=255)
                                                                                                          aCTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTATAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGATTCTGAACCAAAAGAAGAc                    >  1:1278128/1‑128 (MQ=255)
                                                                                                             ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa                 <  1:3926157/128‑1 (MQ=255)
                                                                                                               cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa               >  2:582090/1‑128 (MQ=255)
                                                                                                               cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa               >  1:2642592/1‑128 (MQ=255)
                                                                                                               cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa               <  2:1722521/128‑1 (MQ=255)
                                                                                                                tATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaa              >  2:6371541/1‑128 (MQ=255)
                                                                                                                  tttttAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAAc            <  1:6007096/128‑1 (MQ=255)
                                                                                                                      tAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaa        >  2:291547/1‑128 (MQ=255)
                                                                                                                       aaTTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaa       <  1:82173/128‑1 (MQ=255)
                                                                                                                            ataAATTTATATTAAAAAATTTATATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTTAACAAATAGAAGACGAAAAAACAAAAAAAGAt  <  2:6105255/128‑1 (MQ=255)
                                                                                                                               ||                                                                                                                           
TGAGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAGAT  >  CP016816/19896‑20147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: