BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.4%	7952.4 / NA	2499	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (8/6); new base T (1303/1181); total (1312/1187)
Fisher's exact test for biased strand distribution p-value = 7.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	98.7%	7509.3 / NA	2500	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (7/26); new base T (1305/1162); total (1312/1188)
Fisher's exact test for biased strand distribution p-value = 3.13e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2602 total aligned reads displayed.

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑ACAAAAATATAATTTATTTGATTAAACGTAT  >  CP016816/420031‑420282

||

     aaattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattt                                                                                                                             <  1:2230427/128‑2 (MQ=255)

       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAATTttttaatttta                                                                                                                           <  1:3185597/128‑4 (MQ=255)

        ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                          >  1:6314511/1‑124 (MQ=255)

           attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                       >  2:5518880/1‑128 (MQ=255)

           attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                       <  1:263/128‑1 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                      >  2:4960102/1‑128 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                      >  2:3960733/1‑128 (MQ=255)

              catACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTg                                                                                                                    <  2:4472130/128‑1 (MQ=255)

                  cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATAATTTATAAAAATAATACTATTATTTGTTGATTTATTAGATAACTACTAGCTCTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                <  2:620436/128‑1 (MQ=255)

                   ttttAGTATCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                               <  2:1933947/128‑1 (MQ=255)

                    tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                              >  1:3472562/1‑128 (MQ=255)

                    tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                              >  1:4136235/1‑128 (MQ=255)

                          ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                        >  2:4175325/1‑128 (MQ=255)

                          ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                        >  2:3390158/1‑128 (MQ=255)

                           tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                       <  2:6791732/128‑1 (MQ=255)

                           tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTACaa                                                                                                       >  2:1143488/1‑128 (MQ=255)

                           tcATAAATCTAAGTTTCTAAAATTAATTTATCATTTAAAAAACTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                       <  1:4380596/128‑1 (MQ=255)

                           tcATAAATATAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                       <  2:3660858/128‑1 (MQ=255)

                            cATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                      <  1:3051929/128‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                     <  1:1001772/128‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                     >  1:3869076/1‑128 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                     <  2:6924998/128‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATAAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                     >  2:5619563/1‑128 (MQ=255)

                               aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTATAATAAATGAATTAATTATAAAATTg                                                                                                   >  2:2155626/1‑128 (MQ=255)

                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                  <  2:3286988/128‑1 (MQ=255)

                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                  <  1:5545135/128‑1 (MQ=255)

                                 aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                 <  2:3501499/128‑1 (MQ=255)

                                  tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                <  2:5296098/128‑1 (MQ=255)

                                   cTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaa                                                                                               <  1:4573696/128‑1 (MQ=255)

                                     aattttCTAAAATTATTTTATGATTTAAAAAAATAATACTTTTAATAGTTGCTATATTAGTAAACTACTTGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                             <  1:2392017/125‑1 (MQ=255)

                                     aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                             <  1:7062636/128‑1 (MQ=255)

                                      aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                            <  1:5319680/128‑1 (MQ=255)

                                       gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                           >  1:6934373/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTGGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                        <  2:770767/111‑1 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                       >  1:508441/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                       <  2:5727544/128‑1 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTATTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                       >  1:6531896/1‑128 (MQ=255)

                                            tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                      <  1:3341251/128‑1 (MQ=255)

                                            tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                      <  1:1888635/128‑1 (MQ=255)

                                               aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                   >  1:6813988/1‑128 (MQ=255)

                                               aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTCAATAATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                   >  2:4641789/1‑128 (MQ=255)

                                                attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAATTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                           <  1:2788124/119‑1 (MQ=255)

                                                attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                  >  2:6307365/1‑128 (MQ=255)

                                                attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                  <  2:4831080/128‑1 (MQ=255)

                                                     tttATTATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                             <  2:2055233/128‑1 (MQ=255)

                                                      ttATCATTTAAAAAAATAATACTTTTATTAGTTTATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                            <  1:6408806/128‑1 (MQ=255)

                                                        aTCATTTAAAAAAATTATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                          <  2:1036710/128‑1 (MQ=255)

                                                        aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                          <  1:3784323/128‑1 (MQ=255)

                                                          cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                        >  2:869119/1‑128 (MQ=255)

                                                           aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt‑t                                                                      >  1:3974910/1‑128 (MQ=255)

                                                           aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt‑t                                                                      >  2:5408837/1‑128 (MQ=255)

                                                             ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑Taa                                                                    <  2:2493170/128‑1 (MQ=255)

                                                             ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACAAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑Taa                                                                    >  1:6677484/1‑128 (MQ=255)

                                                              tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTAT‑TAAt                                                                   >  1:5474713/1‑128 (MQ=255)

                                                                    aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAAttt                                                             <  1:1160941/128‑1 (MQ=255)

                                                                    aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAAttt                                                             <  2:1807279/128‑1 (MQ=255)

                                                                    aataatACTAATAATAGTAGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAAttt                                                             <  1:1468181/128‑1 (MQ=255)

                                                                      taatttttttATTATTTTATATATTTGATATATTTTATAAATTTTTTAAAAAATTTTTATTTTTTATAAATTAATTATTTTTAAAATTGTTAAATTATTTTATTATAATTATTTAT‑TAATGAATTTTa                                                           <  1:5645013/122‑1 (MQ=255)

                                                                         taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGc                                                        >  1:3649002/1‑128 (MQ=255)

                                                                          aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATAT‑TAATCAATTTTAttat                                                       >  2:5104071/1‑125 (MQ=255)

                                                                           cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGATc                                                      >  1:4916998/1‑128 (MQ=255)

                                                                             tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGt                                                    >  1:779012/1‑128 (MQ=255)

                                                                             tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTAAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATAATCTAT‑TAATCAATATTATCCTCga                                                    >  2:3178980/1‑127 (MQ=255)

                                                                              ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGtt                                                   >  1:132680/1‑128 (MQ=255)

                                                                              ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTATAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TATTCAATTTTATGCTCGtt                                                   >  1:4762308/1‑128 (MQ=255)

                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGttt                                                  >  2:2267546/1‑128 (MQ=255)

                                                                                    gTTGATATAATAGAAAACTAAAATCAATTTTTTAAAAAATTTTTAATTTTTATAACTTTATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTAACAAT‑TAAACAATTTTATGCTCGttt‑tt‑ttt                                           <  2:2836368/128‑1 (MQ=255)

                                                                                     ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGttt‑tt‑tttt                                          >  1:6236284/1‑128 (MQ=255)

                                                                                        atatatAATAAAAATAATATCAATTTTTAAAAAAATATTTAATTTTAATAACTTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTAACAAT‑TAATCAATTTTATGCCCGttt‑tt‑ttttttt                                       <  2:2743396/128‑1 (MQ=255)

                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAAt                                                                   >  1:2916699/1‑100 (MQ=255)

                                                                                              tactaaaaTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAATTAATTTCAAATCAATATCTAA‑TAATCAATTTTATGCGCGgtt‑tt‑ttttttttttttt                                 <  2:7054016/124‑2 (MQ=255)

                                                                                              tAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTGAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGttt‑tt‑ttttttttttttt                                 >  2:6667440/1‑127 (MQ=255)

                                                                                                ttaaaaTAATATCAATTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTAAAATTTTTAAATTATTTTTAAATCATTATATAT‑TAATCAATTTTATGCGCGTTT‑TT‑TTTTTTTTTTTT‑ACa                              <  2:4341142/126‑1 (MQ=255)

                                                                                                 aaaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGttt‑tt‑tttttttttttaaaaa                              >  1:5787264/1‑123 (MQ=255)

                                                                                                 aaaaATACTATCAATTTTTTAAAAAATTTTTAATTTTAAAAAATTAATATTTTTTAAAATTTTTAAATTTTTTTAAAAAAATTAT‑TATATAATAAATTTTATTCCCGTTT‑TT‑TTTTTTTTTTTT‑ACaa                             <  2:7195544/128‑1 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTa‑aaaaa                            >  2:2386720/1‑128 (MQ=255)

                                                                                                  aaaCTACAAGCACTTTTTTAAAAAATTTTTAATTTTAAAAAATGAAATATTTTTAAAATTTTTAAATTTTTTTCAAAACATTATCTAT‑TAATAAATTTTATGCGCGTTT‑TT‑TTTTTTTTTTTT‑ACaaa                            <  2:2590436/128‑1 (MQ=255)

                                                                                                    tataatatatattttttAAAAAATTTTTAATTTTAATTAATTAAATTTTTTTAAAATTTTTAAATTATTTTAAAATAATTATATAT‑TAATAAATTTTTTTCTCGTTT‑TT‑TTTTTTTTTTTT‑ACaaaaa                          <  2:1274258/117‑1 (MQ=255)

                                                                                                    aatattttaaattttttAAAAAATTTATAATTTTTATAAATTAATTTTTTTTAAAATTTTTAAATTATTTTAAAATAAATATATAT‑TAATAAATTTTATTGTCGTTT‑TT‑TTTTTTTTTTTT‑ACaaaaa                          <  2:2966324/117‑1 (MQ=255)

                                                                                                     ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑ACAAAAat                         >  2:6551777/1‑128 (MQ=255)

                                                                                                        ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATAAATTTTATCCTCTTTT‑TTATTTTTTTTTTTT‑Aaaaaaaaaa                       >  2:1698770/1‑125 (MQ=255)

                                                                                                        atatcaattttttAAAAAATTTTTATTTTTAATTAATTAATTATTTTTAAAATTTTTTAATTATTTTAAAATAATTTTATAT‑TAATAAATTTTATTCTTG‑TT‑TT‑TTTTTTTTTTTT‑ACAAAAATATaa                     <  1:2514682/121‑1 (MQ=255)

                                                                                                         tatcaattttttAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTTTTAAATTATTTTAAAATCAATTTATAT‑TAATAAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑ACAAAAATATaa                     <  1:6014649/122‑1 (MQ=255)

                                                                                                         tatcaattttttAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTTTTAAATTATTTTAAAATCATTATATAT‑TAATAAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑ACAAAAATATaa                     <  2:6427067/122‑1 (MQ=255)

                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCG‑TT‑TT‑TTTTTTTTTTTT‑ACAAAAATATaaat                   >  2:5853807/1‑126 (MQ=255)

                                                                                                           gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑AAAAAAAAAAAatt                   >  2:1400891/1‑128 (MQ=255)

                                                                                                            cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑AAAAAAATATAtttt                  >  1:5908074/1‑128 (MQ=255)

                                                                                                            cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATCTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑AAAAAAATAAAattt                  >  1:6129971/1‑128 (MQ=255)

                                                                                                              cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑AAAAAAATA‑Aatttatt               >  1:1632880/1‑128 (MQ=255)

                                                                                                               ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTGAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑AAAAAAATTTAatttatt               >  1:1762750/1‑128 (MQ=255)

                                                                                                               ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑AAAAAAATATAatttatt               >  2:3773016/1‑128 (MQ=255)

                                                                                                                 ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCTTTT‑TT‑TTTTTTTTTTTTAAAAAAAATAAAatttattt              >  2:5959106/1‑128 (MQ=255)

                                                                                                                 ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑ACAAAAATATAatttatttt             >  2:4081808/1‑127 (MQ=255)

                                                                                                                    ttaaaaaTTTTTAATTTTAAAAACTTAATTATTTTTTAAATTTTTAAATTATTTTAAAATAATTATTTAT‑TAATAAATTTTATTCTGGTTT‑TT‑TTTTTTTTTTTT‑ACAAAAATATAATTTATTTGAtt          <  1:871961/126‑1 (MQ=255)

                                                                                                                    tAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCG‑‑T‑TT‑TTTTTTTTTTTT‑ACAAAAATATAATTTATTTGATTaa        >  2:3066182/1‑128 (MQ=255)

                                                                                                                    tAAAAAAATTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTATCTAT‑TAATCAATTTTTTGCTCGTTT‑TT‑TTTTTTTTTTTT‑ACAAAAATATAATTTATTTGAtt          <  2:6095279/128‑1 (MQ=255)

                                                                                                                      aaaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑AAAAAAATATAATTTATTTGATTaa        >  2:152686/1‑128 (MQ=255)

                                                                                                                       aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑AAAAAAATAAAATTTATTTAATTaaa       >  1:2023303/1‑128 (MQ=255)

                                                                                                                       aaaaTTTTTAATTTTAATAACTGAAATATTTTTCAAATTGTTAAATTATTTACAAATCATTATCTAT‑TAATCATTTTTATGCTCGTTTATT‑TTTTTTTTTTTA‑ACAAAAATAAAattaaatttataaa        >  2:3858068/1‑117 (MQ=255)

                                                                                                                         aaTTTTAAATTTAAAAAATTTAATT‑TTTTTTAAATTTTTAAAATTTTTTTAAATAATTTTATAT‑TAAAAATTTTTTTTTTAGTTT‑TT‑TTTTTTTTTTTT‑ACAAAAATATAATTTATTTGATTAAACGt    <  1:7200547/128‑1 (MQ=255)

||

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAT‑TAATCAATTTTATGCTCGTTT‑TT‑TTTTTTTTTTTT‑ACAAAAATATAATTTATTTGATTAAACGTAT  >  CP016816/420031‑420282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`