BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,163	2 bp→TT	intergenic (‑446/+396)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,163	0	A	T	99.4%	7853.8 / NA	2500	intergenic (‑446/+397)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (9/7); new base T (1303/1177); total (1312/1188)
Fisher's exact test for biased strand distribution p-value = 8.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,164	0	A	T	99.2%	6929.7 / NA	2475	intergenic (‑447/+396)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (8/13); new base T (1290/1162); total (1299/1176)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2506 total aligned reads displayed.

ATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTA  >  CP016816/420038‑420287

||

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                             <  1:146192/128‑1 (MQ=255)

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                             <  2:4371015/128‑1 (MQ=255)

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                             <  1:7079597/128‑1 (MQ=255)

    attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                            >  2:5864231/1‑128 (MQ=255)

    attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACCAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                            >  2:2066614/1‑128 (MQ=255)

     ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                           <  1:5908773/128‑1 (MQ=255)

       catACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTg                                                                                                                         <  2:5978272/128‑1 (MQ=255)

         tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                       <  1:4394780/128‑1 (MQ=255)

         tACTTTTAGTCTCATAAATCTAAGTTTATAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                       <  1:4764708/128‑1 (MQ=255)

          aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATAACTTTTTTAAAAAATTTTTAATATTAATAACTGaaa                                                                                                                      >  2:3548193/1‑127 (MQ=255)

            ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                    <  2:1529062/128‑1 (MQ=255)

              ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                  <  2:7197787/128‑1 (MQ=255)

               tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                                 >  2:3193457/1‑128 (MQ=255)

               tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                                 <  1:2519665/128‑1 (MQ=255)

                aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                                >  1:5657613/1‑128 (MQ=255)

                  tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAATCTACTAGCACTTTTTTATAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                              <  2:2279057/128‑1 (MQ=255)

                  tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                              <  1:3078795/128‑1 (MQ=255)

                  tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                              >  2:4845731/1‑128 (MQ=255)

                    tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTACaa                                                                                                            >  2:1143488/1‑128 (MQ=255)

                     cATAAATATAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                           >  1:2232702/1‑128 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  2:3977516/128‑1 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          >  1:5326791/1‑128 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  1:1001772/128‑1 (MQ=255)

                      aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                          <  1:3901232/128‑1 (MQ=255)

                       tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                         <  2:5657026/128‑1 (MQ=255)

                        atatagaattttCTAAAATTATTTTATAATTTAAAAAAATAATAATTTTATTAGTTTATATATTAGAAAAATAATATAACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAAttt                                                                                                        <  2:6308514/119‑2 (MQ=255)

                        aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAttt                                                                                                        >  1:3660560/1‑127 (MQ=255)

                        aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAATTg                                                                                                        >  2:6793285/1‑128 (MQ=255)

                         aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                       >  2:2394229/1‑128 (MQ=255)

                           tCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTa                                                                                                     >  1:5482154/1‑128 (MQ=255)

                               aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                 <  2:6111288/128‑1 (MQ=255)

                                  ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                            <  1:3348135/114‑1 (MQ=255)

                                   tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                             <  1:784560/128‑1 (MQ=255)

                                   tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                             >  1:1755813/1‑128 (MQ=255)

                                    cTATAATTATTTTATAATTTAAAATAATATTACTTTTATTAGTTTATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTAtttt                                                                                            <  1:681803/128‑1 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                            >  2:1407288/1‑128 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                            >  1:508441/1‑128 (MQ=255)

                                     tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                           <  2:6663177/128‑1 (MQ=255)

                                     tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                           <  2:3292088/128‑1 (MQ=255)

                                       aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACAGAATTATTTTTCAAATTTTTAAATTATTTTaaa                                                                                         >  2:4667689/1‑128 (MQ=255)

                                        aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                        <  1:7203072/128‑1 (MQ=255)

                                         attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                       >  2:3405539/1‑128 (MQ=255)

                                         attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                       <  2:5442666/128‑1 (MQ=255)

                                            attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                                    <  1:6316821/128‑1 (MQ=255)

                                             ttttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAtt                                                                                   <  2:2988062/128‑1 (MQ=255)

                                              tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                  <  1:2796943/128‑1 (MQ=255)

                                              tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                  >  1:5794253/1‑128 (MQ=255)

                                               ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                                 <  2:2165318/128‑1 (MQ=255)

                                               ttATCATTTAAAAAAATAATACTTTTAATAGTTGATATATTAGAAAAATACTAGCACTATTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTGTTATATTATTTTCAAATCATAAt                                                                                 >  1:878005/1‑128 (MQ=255)

                                                tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTTTc                                                                                >  1:2933788/1‑128 (MQ=255)

                                                tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                <  1:2648061/128‑1 (MQ=255)

                                                 attaTTTTAAAAAATAATACTTTTATTAGTTGATATTTTAGATATCTACTATCAATTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               <  1:5549530/125‑1 (MQ=255)

                                                 aTCATATAAAAATATAATACTTTTATTATTTGATTAATTAGAATACTACTTGCACTTTTTTAAAAAATTTATAATTTTAATAACTGAATTATATTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                               <  2:4518147/128‑1 (MQ=255)

                                                   cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                             >  2:78089/1‑128 (MQ=255)

                                                   cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                             >  2:869119/1‑128 (MQ=255)

                                                   cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTAAAATAGTTAAATTATTTTCAAATCATTATCTAt                                                                             >  1:1158710/1‑128 (MQ=255)

                                                   cATTTAAAAAAATAATACTTTTATTAGATGAAATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTATTCAAATTGTTAAATTAATATCAAATCATTATCTAt                                                                             >  2:3884826/1‑128 (MQ=255)

                                                      ttAAAAAAATAATACTTTTATTAGTTGATATATTAGTAAACTACTAGCACTTTTTTAAAAAATTTTTAAGTTTAATATCTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                          <  2:1044351/128‑1 (MQ=255)

                                                      ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                          <  2:2493170/128‑1 (MQ=255)

                                                      ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                          >  1:6945843/1‑128 (MQ=255)

                                                        aaaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATc                                                                        >  2:6431662/1‑128 (MQ=255)

                                                             aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                   <  2:1808863/128‑1 (MQ=255)

                                                                  taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGc                                                            >  1:6704590/1‑128 (MQ=255)

                                                                   aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                           <  1:1326689/98‑1 (MQ=255)

                                                                   aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TTC‑t                                                          >  2:2749107/1‑128 (MQ=255)

                                                                     ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TTC‑TCg                                                        >  2:5533659/1‑128 (MQ=255)

                                                                     ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATAAATTTTA‑‑TGC‑TCg                                                        >  1:6237212/1‑128 (MQ=255)

                                                                      tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TTC‑TCG‑t                                                      >  1:3787282/1‑128 (MQ=255)

                                                                        tattatTTTATATATTATAAAACTAATAGAAATTTTTTAAAAAATTTTTAATTTTAATAAATTTATTATTTTTTAAATTGTTAAATTATTTTTAAATAATTATCTTTTAATAAATTTTT‑‑TTC‑TCG‑ttt                                                    <  1:2394229/128‑1 (MQ=255)

                                                                        tattaGTTGTTATATTATAAAACTAATATCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAATTTTT‑‑TGC‑TCG‑ttt                                                    <  2:1272559/128‑1 (MQ=255)

                                                                        tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑ttt                                                    >  2:4096839/1‑128 (MQ=255)

                                                                               tGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                           <  1:6017213/86‑1 (MQ=255)

                                                                               tGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑tttttttttt                                             >  2:5744203/1‑128 (MQ=255)

                                                                                 atatatTATAAAAATAATATCACTTTTTTAAAAAATTTTTAATATTAATAACTTAATTATTTTTCAAATTTTTAAATTATTTTAAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑tttttttttttt                                           <  2:3668947/128‑1 (MQ=255)

                                                                                   atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑tttttttttttttt                                         >  1:1620722/1‑128 (MQ=255)

                                                                                    ttttAGAAAAATAATAATACTTTTTTAAAAAATTTTTATTTTTAATAAATTAATTTTTTTTAAAATTTTTAATTTATTTTCAAATAATTATAAATTAATAAATTTTA‑‑TTC‑TCt‑ttttttttttttttt                                        <  1:400289/126‑1 (MQ=255)

                                                                                     atTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑tttttttttttttttt                                       >  2:4994574/1‑128 (MQ=255)

                                                                                      ttAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCt‑ttttttttttttttttt                                      >  2:5143569/1‑128 (MQ=255)

                                                                                       tactaaaaTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAATTAATTTCAAATCAATATCTAATAATCAATTTTA‑‑TGC‑GCG‑gttttttttttttttttt                                     <  2:7054016/124‑2 (MQ=255)

                                                                                         gAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑ttttttttttttttttttaa                                   >  1:3204212/1‑125 (MQ=255)

                                                                                           aaaCTACAAGCACTTTTTTAAAAAATTTTTAATTTTAAAAAATGAAATATTTTTAAAATTTTTAAATTTTTTTCAAAACATTATCTATTAATAAATTTTA‑‑TGC‑GCG‑TTTTTTTTTTTTTTTTTACaaa                                 <  2:2590436/128‑1 (MQ=255)

                                                                                            aatatttttaatttttttAAAATATTTTATTTTTAAA‑AAATTATTTTTTTTTAAAATTTTTAAATTATTTTAAATTAATTTTTTTTTAATAATTTTTATTTAT‑TTT‑TTTTTTTTTTTTTTTTTACaaa                                 <  1:459167/116‑1 (MQ=255)

                                                                                            aaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑tttttttttttttttaaaaaaaa                                >  1:653368/1‑120 (MQ=255)

                                                                                             aataatatgaattttttAAAAAATTTTTAATTTTAATTAATTTATTATTTTTTAAATTTTTAAATTTTTTTTAAATTATTATTTATTAATAAATTTTT‑‑TTT‑TTG‑TTTTTTTTTTTTTTTTTACaaaaa                               <  1:1873765/117‑1 (MQ=255)

                                                                                              ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑TTTTTTTTTTTTTTTTTACAAAAat                              >  2:6551777/1‑128 (MQ=255)

                                                                                              ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑‑TTTTTTTTTTTTTTTTAAAAAAata                             >  1:640952/1‑128 (MQ=255)

                                                                                              ataatattatttttttAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTT‑‑TTC‑TCGTTTTTTTTTTTTTTTTTTACaaaaa                               <  1:4161021/118‑1 (MQ=255)

                                                                                               tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑TTTTTTTTTTTTTTTTTAAAAAAata                             >  2:4215530/1‑128 (MQ=255)

                                                                                                actaTCAATTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATCAATTTTA‑‑TGC‑TCG‑‑‑TTTTTTTTTTTTTTTACAAAAATATaa                          <  2:5326791/128‑1 (MQ=255)

                                                                                                 atttaattttttaAAAAAATTTTAAATTTAAAAAAATTAA‑TATTTTTTAAAATTTTAAATTTTTTTTAAAAAAATATTTATTTAAAAAATTTA‑‑ATC‑TCG‑TTTTTTTTTTTTTTTTTACAAAAATATaa                          <  2:6939745/121‑1 (MQ=255)

                                                                                                 atttaaattttttAAAAAATTTTTTATTTTATTAAATTATTTTTTTTTTAAATTTTTTAATTATTTTTAAATATTTATTTATTAATAAATTTTT‑‑TTT‑TAT‑TTTTTTTTTTTTTTTTTACAAAAAtata                           <  1:4917169/121‑1 (MQ=255)

                                                                                                    gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑‑TTTTTTTTTTTTTTTTAAAAAAATAAAattt                       >  2:1941257/1‑128 (MQ=255)

                                                                                                     cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATCTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑TTTTTTTTTTTTTTTTTAAAAAAATAAAattt                       >  1:6129971/1‑128 (MQ=255)

                                                                                                     aatatttttAAAAAATTTTTAATTTTAATAAATTAATTATTTTTTAAATTTTTAAATTTATTTAAAATAATTATTTATTTATAAATTTTT‑‑TGC‑TCG‑TTTTTTTTTTTTTTTTTACAAAAATATAattt                       <  2:3089364/124‑1 (MQ=255)

                                                                                                      atttttttAAAAAATTTTTAATTTTAATAACTTAAATATTTTTTAAATTTTTAAATTATTTTAAAAAAATTATATATTAATCAATTTTA‑‑TGC‑TCG‑‑TTTTTTTTTTTTTTTTACAAAAATATAatttat                     <  2:1703055/126‑1 (MQ=255)

                                                                                                        ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑TTTTTTTTTTTTTTTTTAAAAAAATTAAatttatt                    >  2:6214886/1‑128 (MQ=255)

                                                                                                          ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TTC‑TCG‑‑TTTTTTTTTTTTTTTTAAAAAAATATAatttatttta                 >  1:3502207/1‑126 (MQ=255)

                                                                                                             ttaaaaaTTTTTAATTTTAATAAATTTATTTTTTTTTAATTTTTTAAATTATTTTATAATAATTATATATTAATAAATTTTA‑‑TTC‑TAG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt               <  2:2844207/126‑1 (MQ=255)

                                                                                                               aaaaaTATTAAATTTTAATAAAGTTATTTTTTTTAAAATT‑TTAAATTTTTTTTAAATTAATTTATATTTATTAATTTTT‑‑TTCTTTT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaa             <  2:3783021/128‑1 (MQ=255)

                                                                                                                aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑TTTTTTTTTTTTTTTTTAAAAAAATAAAATTTATTTAATTaaa            >  1:2023303/1‑128 (MQ=255)

||

ATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTA‑‑TGC‑TCG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTA  >  CP016816/420038‑420287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`