Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 431,110 G→A T140I (ACT→ATT)  JCVSYN2_01955 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816431,1100GA99.4% 15001.0 / 27.2 4707T140I (ACT→ATT) JCVSYN2_01955hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (16/12);  new base A (2549/2101);  total (2583/2124)
Fisher's exact test for biased strand distribution p-value = 8.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.31e-01

Only 100 of 4833 total aligned reads displayed.

AGCTAATAAAGTTTGTTTAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAA  >  CP016816/430983‑431236
                                                                                                                               |                                                                                                                              
  cTAATAAAGTTTGTTTAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATa                                                                                                                              >  2:6298781/1‑128 (MQ=255)
   taataaAGTTTGTTTAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACAACtaataa                                                                                                                             >  2:6111722/1‑128 (MQ=255)
     ataaAGTTTGTTTAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAAtt                                                                                                                           >  2:9874265/1‑128 (MQ=255)
       aaaGTTTGTTTAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTATTAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAg                                                                                                                         <  2:1230075/128‑1 (MQ=255)
           tttgtttAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGga                                                                                                                     <  1:3867923/128‑1 (MQ=255)
             tgtttAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGgaga                                                                                                                   <  2:219260/128‑1 (MQ=255)
             tgtttAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGgaga                                                                                                                   >  2:4348928/1‑128 (MQ=255)
              gtttAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGaa                                                                                                                  >  1:1965913/1‑128 (MQ=255)
                ttAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAAt                                                                                                                <  1:7451200/128‑1 (MQ=255)
                 tAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAAtt                                                                                                               >  2:1613653/1‑128 (MQ=255)
                 tAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAAtt                                                                                                               >  1:1355740/1‑128 (MQ=255)
                  tggtactcaatttAATAAGAAAGCACTTATTTCTCAAAACATTAAAGTAGATGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATAAGTGGAGAAATTg                                                                                                              <  1:3668882/117‑1 (MQ=255)
                  aGTTCCTAAAATTAAAAAGACTGAACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTg                                                                                                              <  1:2300412/128‑1 (MQ=255)
                    ttCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATAGCa                                                                                                            >  1:6084432/1‑128 (MQ=255)
                     tCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCaa                                                                                                           >  2:5208002/1‑128 (MQ=255)
                     tCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAAATCCTCATTTAATTGATTCACCACTAATAATTAGTCGAGAAATTGCaa                                                                                                           >  2:2698031/1‑128 (MQ=255)
                      ccTAATATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAAt                                                                                                          <  2:4766088/128‑1 (MQ=255)
                         aaaaTTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTg                                                                                                       <  1:5729296/128‑1 (MQ=255)
                         aaaaTTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCATCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTg                                                                                                       <  1:5014936/128‑1 (MQ=255)
                           aaTTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTa                                                                                                     <  1:2691388/128‑1 (MQ=255)
                             ttAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGt                                                                                                   <  1:9661131/128‑1 (MQ=255)
                              tAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTg                                                                                                  <  1:441979/128‑1 (MQ=255)
                              tAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACAAATAATTAGTGGAGAAATTGCAATTTGTAGTg                                                                                                  >  1:5903561/1‑128 (MQ=255)
                                     aCTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATaa                                                                                           >  2:4047412/1‑128 (MQ=255)
                                     aCTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATaa                                                                                           >  1:324600/1‑128 (MQ=255)
                                      cTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATaaa                                                                                          >  2:2061321/1‑128 (MQ=255)
                                      cTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATaaa                                                                                          >  1:9295303/1‑128 (MQ=255)
                                      cTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATaaa                                                                                          >  1:76006/1‑128 (MQ=255)
                                      cTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTCGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATaaa                                                                                          >  2:1051376/1‑128 (MQ=255)
                                        gCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGTAGAAATTGCAATTTGTAGTAGATATAAAAt                                                                                        >  1:3093212/1‑128 (MQ=255)
                                        gCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAAt                                                                                        <  1:46070/128‑1 (MQ=255)
                                         cACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATa                                                                                       <  1:10000665/128‑1 (MQ=255)
                                         aaCTTAATTCTCAATACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATa                                                                                       <  1:3442801/127‑1 (MQ=255)
                                           cTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGa                                                                                     >  2:8649473/1‑128 (MQ=255)
                                            ttAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGat                                                                                    <  2:8252971/128‑1 (MQ=255)
                                            ttAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGat                                                                                    <  2:3519341/128‑1 (MQ=255)
                                              taTTCTCAAAACTTTAAAGTAGTAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGataa                                                                                  <  1:7082357/127‑1 (MQ=255)
                                              aaTTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGataa                                                                                  >  1:2892895/1‑128 (MQ=255)
                                               aTTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGataat                                                                                 >  2:8863904/1‑128 (MQ=255)
                                                 tctcAAATCTTTAAATTAGAAGTTGTTTTAGAAAAAGGTTTATTAAAATAAATTCATCATTTAATTGATTCAACACTAATAATTAGTTGAGAAATTGCAATTTGTAGTGGATATAAAATAGAtaataa                                                                               <  2:4560796/128‑1 (MQ=255)
                                                 tctcAAAACATTAAAGTAGAAGTTGGTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGAtaataa                                                                               <  1:4033247/128‑1 (MQ=255)
                                                  ctcAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACAACTAATAATTAGTTGAGAAATTGCAAATTGTAGTGGATATAAAAAAGAAAATaaa                                                                              >  2:5366065/1‑128 (MQ=255)
                                                      aaaCTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATaaaaaaa                                                                          >  2:7130707/1‑128 (MQ=255)
                                                       aaCTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATTAAAAAAc                                                                         >  1:9111216/1‑128 (MQ=255)
                                                         cTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAATCTa                                                                       >  1:6667295/1‑128 (MQ=255)
                                                         cTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTa                                                                       >  2:7738131/1‑128 (MQ=255)
                                                         cTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTa                                                                       <  2:6503440/128‑1 (MQ=255)
                                                         cTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTa                                                                       >  2:8432786/1‑128 (MQ=255)
                                                            tAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTaaaa                                                                    >  2:7891746/1‑128 (MQ=255)
                                                            tAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTaaaa                                                                    >  2:9068929/1‑128 (MQ=255)
                                                             aaaGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTa                                                                                                                          >  2:4975206/1‑73 (MQ=255)
                                                               aGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAaaataa                                                                 <  1:9855469/128‑1 (MQ=255)
                                                                 tAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAaaataaat                                                               >  2:2870892/1‑128 (MQ=255)
                                                                 tAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAaaataaat                                                               >  1:7825461/1‑128 (MQ=255)
                                                                  aGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCATTTTGTAGTGGATATAAAATAGATAATAAAAAAACTaaaaatactc                                                              >  1:4236757/1‑122 (MQ=255)
                                                                  aGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATc                                                              >  1:8733898/1‑128 (MQ=255)
                                                                  aGAAGTTGGTTTTGATAAAGGGGGAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATc                                                              <  1:10381191/128‑1 (MQ=255)
                                                                   gAAGTTGTTTTTGATAAATGTGTTGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATcc                                                             <  2:5539393/128‑1 (MQ=255)
                                                                   gAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCGATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATcc                                                             >  1:819892/1‑128 (MQ=255)
                                                                   gAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATcc                                                             >  1:1541268/1‑128 (MQ=255)
                                                                   gAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATcc                                                             >  2:6731427/1‑128 (MQ=255)
                                                                   gAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATcc                                                             <  1:644881/128‑1 (MQ=255)
                                                                   gAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATcc                                                             >  2:3767566/1‑128 (MQ=255)
                                                                    aaGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGTAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCa                                                            >  2:3085743/1‑128 (MQ=255)
                                                                    aaGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCa                                                            >  2:9492599/1‑128 (MQ=255)
                                                                    aaGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCa                                                            <  1:5374556/128‑1 (MQ=255)
                                                                     aGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAg                                                           >  2:10337691/1‑128 (MQ=255)
                                                                        tgttTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATATATAATAAAAAAACTAAAATAAATCCAGaaa                                                        >  1:4796189/1‑128 (MQ=255)
                                                                         gttTTTGATAAAGGTGTAGTAAAATCAATTCATCATATAATTGATTAACAACTAATAATTATTGGAGAAATTGCAAATATTAGTAGATATAAAAAAGATAATAAAAAAACTAAAATAAATACACAAAc                                                       >  2:10179977/1‑128 (MQ=255)
                                                                             ttGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAAtt                                                   <  2:9665413/128‑1 (MQ=255)
                                                                                attaaagtgtAGTAAAATCAATTCCTCATTTAAATGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTtta                                                <  2:5718258/122‑1 (MQ=255)
                                                                                aTAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTtta                                                <  1:8930053/128‑1 (MQ=255)
                                                                                      gtgtAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTttattatta                                          <  1:7999187/128‑1 (MQ=255)
                                                                                       tgtAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGTGAAATTGCAATTTGTAGTGGATATTAAATAGATAATAAAAAATCTAAAATAAATCCAGAAACAATTTtattattat                                         >  1:1799746/1‑128 (MQ=255)
                                                                                       tgtAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTtattattat                                         <  1:5208002/128‑1 (MQ=255)
                                                                                       tgtAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTtattattat                                         >  2:7331276/1‑128 (MQ=255)
                                                                                        gtagtaAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTTCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCAAGAAACAATTTTATTATtata                                        >  2:7507335/1‑128 (MQ=255)
                                                                                        gtagtaAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGTATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATtata                                        >  2:10030484/1‑128 (MQ=255)
                                                                                          agtaAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATATTATAAAATCTTAATTAAATGCTGAAACAATTTTATTATTTTAAt                                      >  2:1833217/1‑128 (MQ=255)
                                                                                          agtaAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAATAACTAAAATAAATCCAGAAACAATATTATTATTATAAt                                      >  2:9229928/1‑128 (MQ=255)
                                                                                          agtaAAATCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAAt                                      <  2:8082630/128‑1 (MQ=255)
                                                                                              aaaTCAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGAAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCt                                  <  1:7270690/128‑1 (MQ=255)
                                                                                                  cAATTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCttttt                              >  1:628257/1‑128 (MQ=255)
                                                                                                   aaTTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTg                             >  2:3268685/1‑128 (MQ=255)
                                                                                                    aTTCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGc                            <  1:8501745/128‑1 (MQ=255)
                                                                                                    aTTCCTCATTTAATTGATTAACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTTGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGc                            <  1:1139877/128‑1 (MQ=255)
                                                                                                     ttCCTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCa                           <  2:4204039/128‑1 (MQ=255)
                                                                                                       ccTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGt                         <  2:2332118/128‑1 (MQ=255)
                                                                                                       ccTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAAATAAAATAAATACAGAAACAAATTTATTATAATAATTTCTTTTTACatt                         >  1:5561397/1‑126 (MQ=255)
                                                                                                        cTCATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGtt                        <  1:2460318/128‑1 (MQ=255)
                                                                                                          cATTTAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGa                      >  1:94933/1‑128 (MQ=255)
                                                                                                            tttAATTGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGaaa                    >  2:693787/1‑128 (MQ=255)
                                                                                                            tttAATTGATTCACCACTAATAAATAGTGGAGAAATTGCAATTAGTAGTTGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATAATAATTTCTATTATCAGTTaaaa                    >  2:1452245/1‑128 (MQ=255)
                                                                                                                 ttGATTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAAAAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAAtt               >  1:6845508/1‑128 (MQ=255)
                                                                                                                    ttacaccacTAATAATTATTGGAGTAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATACAGAAAAAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCa            <  1:2126746/125‑1 (MQ=255)
                                                                                                                    aTTCACCACTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCa            >  1:8344897/1‑128 (MQ=255)
                                                                                                                          cacTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACt      >  2:5884688/1‑128 (MQ=255)
                                                                                                                          cacTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACt      >  2:2507748/1‑128 (MQ=255)
                                                                                                                           acTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTTTTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTg     >  1:10208481/1‑128 (MQ=255)
                                                                                                                            cTAATAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGt    >  2:3926695/1‑128 (MQ=255)
                                                                                                                               |                                                                                                                              
AGCTAATAAAGTTTGTTTAGTTCCTAAAATTAAAAAGACTGCACTTAATTCTCAAAACTTTAAAGTAGAAGTTGTTTTTGATAAAGGTGTAGTAAAATCAATTCCTCATTTAATTGATTCACCACTAGTAATTAGTGGAGAAATTGCAATTTGTAGTGGATATAAAATAGATAATAAAAAAACTAAAATAAATCCAGAAACAATTTTATTATTATAATTTCTTTTTGCAGTTGAAAAAATTTCATTAACTGTAA  >  CP016816/430983‑431236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: