BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	478,738	C→A	G301V (GGT→GTT)	JCVSYN2_02210 ←	DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	478,738	0	C	A	99.2%	12445.3 / NA	4245	G301V (GGT→GTT)	JCVSYN2_02210	DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand): ref base C (18/14); new base A (2143/2042); total (2169/2076)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4360 total aligned reads displayed.

TGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAATTT  >  CP016816/478612‑478865

  tAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacac                                                                                                                              >  2:7547852/1‑128 (MQ=255)

   aaCATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacacc                                                                                                                             >  2:2564605/1‑128 (MQ=255)

    aCATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAacacca                                                                                                                            >  1:4319905/1‑128 (MQ=255)

     gaTAAGTTTTTCATTATTTACAATAATTGATGGTTCACTCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGTTTCTTATATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                           <  2:98219/127‑1 (MQ=255)

     cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTGTTTTAATCTTTCATTTCTAATACTGATTCTTCTATATAAATCAGTAAGTTCAGAAGTAGTTAATCTACCACCAt                                                                                                                           >  1:3820995/1‑128 (MQ=255)

       tACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCt                                                                                                                         <  2:5571966/128‑1 (MQ=255)

            ttttCTTTATTTACAATAATTGATGCTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTg                                                                                                                    <  1:1679986/128‑1 (MQ=255)

             tttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGa                                                                                                                   <  2:8837783/128‑1 (MQ=255)

             tttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGa                                                                                                                   >  1:1136676/1‑128 (MQ=255)

             tttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGa                                                                                                                   >  1:9728349/1‑128 (MQ=255)

              ttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaa                                                                                                                  >  1:207896/1‑128 (MQ=255)

                cATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTa                                                                                                                        >  2:9235370/1‑120 (MQ=255)

                cATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                                >  2:6708235/1‑128 (MQ=255)

                cATTATTTACAATAATTGATGGTGAACCCATTTCTTTAACTTTTTTTATTCTTTCATTTCAAATAATGATTCTTCTATATAAATCATTAATTACAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                                >  1:6744537/1‑128 (MQ=255)

                 attattTACAATAATTGATGTTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATAAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataa                                                                                                               >  2:7218353/1‑128 (MQ=255)

                 attattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataa                                                                                                               <  1:3967758/128‑1 (MQ=255)

                  ttattTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaataat                                                                                                              <  2:4991370/128‑1 (MQ=255)

                   tatttcaattaatTGAGGGTGCACACAGATCTTTAACTTTTTTTAATCTTTCCTTTCTAATAAAGATTCTTCTATATAAATCATTAATTTCTGAAGTAGTAAATCTAACACCATCTAATTGAATAAtt                                                                                                             <  2:10119143/119‑1 (MQ=255)

                     tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                           >  1:9260562/1‑128 (MQ=255)

                     tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                           >  1:6199040/1‑128 (MQ=255)

                     tttACAATAATTGATGGTGCACCCATTTCTATAACTTTTTTTAATCTTTCATTTCTAATAATGATTATTCTATATAAATCAATAATTTCAGAAGTAGTAAATCTAACACAATCTAAATGaataatacg                                                                                                           >  2:5924030/1‑125 (MQ=255)

                     tttACAATAATTGATGGTGCACCAATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                           <  2:2011835/128‑1 (MQ=255)

                       tACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACTCAATCTAATTGAATAATTGGAc                                                                                                         >  1:10003157/1‑128 (MQ=255)

                       tACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGAc                                                                                                         <  1:9939801/128‑1 (MQ=255)

                        taaataatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACg                                                                                                        >  1:3221096/3‑128 (MQ=255)

                        aCAATTCTTTATGGTGCTCCCATTTCTTTAAGTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCTGAAGTAGTATATGTAAGACCATCTAATTGAATAATTGGACg                                                                                                        <  2:5185945/128‑1 (MQ=255)

                          aataatTGTTGGTGCACACATTTATTTAACATTTTTTAATCTTTTATTTCTAATAATGATTAATCTATATTAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGaa                                                                                                      <  2:4385723/128‑1 (MQ=255)

                          aataatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGaa                                                                                                      >  2:4541179/1‑128 (MQ=255)

                             aatTGATGGTTCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGt                                                                                                   <  2:4213945/128‑1 (MQ=255)

                                tGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtg                                                                                                <  2:1407012/128‑1 (MQ=255)

                                    ggTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggt                                                                                              >  2:3561881/1‑126 (MQ=255)

                                     gTGCACCCAATTCTTTCACTTTTTTTAAGCTTACAATTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTACTTGAATAATTGGACGAATGTCTGGTGGa                                                                                           <  2:7381189/128‑1 (MQ=255)

                                      tGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGaa                                                                                          >  2:7016732/1‑128 (MQ=255)

                                        caaccATTTCTTTTACTTTTTTTAATCTTTAATTTCTAATAATGATTCTTCTATATTAATCTTTAATATGAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                        <  2:3318672/125‑1 (MQ=255)

                                        caaccATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                        <  1:10239487/125‑1 (MQ=255)

                                        cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTTCATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                        >  2:6534256/1‑128 (MQ=255)

                                        cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                        >  2:1775452/1‑128 (MQ=255)

                                        cACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATa                                                                                        >  2:7929435/1‑128 (MQ=255)

                                             aTTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGATTAACTgg                                                                                   >  2:6127604/1‑128 (MQ=255)

                                                tCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAAt                                                                                >  1:1901958/1‑128 (MQ=255)

                                                  tttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTa                                                                              <  1:8351033/128‑1 (MQ=255)

                                                   ttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAc                                                                             >  1:7061026/1‑128 (MQ=255)

                                                   ttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAc                                                                             >  2:8504430/1‑128 (MQ=255)

                                                   ttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAc                                                                             >  1:4858194/1‑128 (MQ=255)

                                                     aaCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAAATGGACGAATCTCTGGTGGAATAACTGGAATTActc                                                                           >  1:6888060/1‑128 (MQ=255)

                                                      attttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActct                                                                          <  2:9029771/126‑1 (MQ=255)

                                                         ttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCAt                                                                       <  2:9444896/128‑1 (MQ=255)

                                                         ttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCAt                                                                       <  2:9628054/128‑1 (MQ=255)

                                                           ttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGATTAACTGGATTTACTCtaataa                                                                     >  1:4147361/1‑128 (MQ=255)

                                                           ttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGCATTACTCTCAGaa                                                                     >  1:2267004/1‑128 (MQ=255)

                                                              tAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACca                                                                  >  1:2469967/1‑128 (MQ=255)

                                                                aTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcatt                                                                >  2:5366621/1‑128 (MQ=255)

                                                                 tCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattc                                                               >  1:7257286/1‑128 (MQ=255)

                                                                  cTTTCATTTCTAATAATGATTCTTCTATATAAACCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattca                                                              <  1:7617455/128‑1 (MQ=255)

                                                                     tCATTTCTTATATTGATTCTTCTATATAAATAATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCattcattc                                                           <  1:3061663/128‑1 (MQ=255)

                                                                     tCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCattcattc                                                           >  1:5018165/1‑128 (MQ=255)

                                                                     tCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCattcattc                                                           >  2:10285553/1‑128 (MQ=255)

                                                                      cATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCAttcattca                                                          <  1:5630068/128‑1 (MQ=255)

                                                                       aTTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAg                                                         <  2:1563663/128‑1 (MQ=255)

                                                                           cTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCAGACAGGACg                                                     >  1:2648851/1‑128 (MQ=255)

                                                                            aaattatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGt                                                    <  1:7479562/127‑1 (MQ=255)

                                                                             aataatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAAAAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGtt                                                   >  1:5839816/1‑128 (MQ=255)

                                                                              ataatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTTAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTg                                                  >  2:2966625/1‑128 (MQ=255)

                                                                                aatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                                <  1:8179036/128‑1 (MQ=255)

                                                                                 atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACTAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                               <  1:3416681/128‑1 (MQ=255)

                                                                                     ttcttcTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATATCTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGaa                                           >  2:1228727/1‑128 (MQ=255)

                                                                                      tcttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAAc                                          >  2:8336161/1‑128 (MQ=255)

                                                                                        ttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGt                                        >  2:6856696/1‑128 (MQ=255)

                                                                                        ttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGGTAGAACGt                                        >  1:5195969/1‑128 (MQ=255)

                                                                                         tctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtt                                       >  1:6046887/1‑128 (MQ=255)

                                                                                          ctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGttt                                      >  1:7845328/1‑128 (MQ=255)

                                                                                           tatataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATACTTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtttt                                     <  1:8002952/128‑1 (MQ=255)

                                                                                           tatataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                                <  1:134641/117‑1 (MQ=255)

                                                                                            atataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTa                                    <  2:5767586/128‑1 (MQ=255)

                                                                                            atataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTa                                    >  1:3619005/1‑128 (MQ=255)

                                                                                            atataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTa                                    <  2:3956871/128‑1 (MQ=255)

                                                                                               taAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAg                                 <  2:10443762/128‑1 (MQ=255)

                                                                                                  atatttAATTTCAGATGTAGTAAATATAACACCATCTAATTTAATAATTGGACTAATGTCTGGTGTAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCAt                              <  1:4686911/124‑1 (MQ=255)

                                                                                                   tCATTACTTTCAGAAGTAGTAAATCTATCACCTTCTAATTGATTAATTGGACGACTGTCTGGTAGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATc                             <  2:7702553/128‑1 (MQ=255)

                                                                                                   tCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATATCCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATc                             >  1:8555726/1‑128 (MQ=255)

                                                                                                       tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                         <  1:5374715/128‑1 (MQ=255)

                                                                                                        aaTTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGTAATAACTGGATTTACTCTCATAAACATTCATTCAGGACGTTGGTTAGAACGTTTTAAATCATCAAAAg                        >  2:2373575/1‑128 (MQ=255)

                                                                                                         aTTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGt                       <  2:865104/128‑1 (MQ=255)

                                                                                                          tttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                                >  1:890548/1‑102 (MQ=255)

                                                                                                          tttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGtt                      >  1:6376897/1‑128 (MQ=255)

                                                                                                            tCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGTAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGCACGTTTTAAAGCTTCAAAAGTTTc                    >  2:2772897/1‑128 (MQ=255)

                                                                                                            tCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTc                    <  2:3761593/128‑1 (MQ=255)

                                                                                                             cAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCt                   <  1:4511414/128‑1 (MQ=255)

                                                                                                              aGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTa                  >  1:9062939/1‑128 (MQ=255)

                                                                                                              aGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTa                  <  1:1477982/128‑1 (MQ=255)

                                                                                                              aGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGGTGGTTAGAACGTTTTAAAGCATCTAAAGTTTCTa                  >  1:655908/1‑128 (MQ=255)

                                                                                                               gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATTACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTaa                 <  2:4769215/128‑1 (MQ=255)

                                                                                                               gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTaa                 <  2:8145909/128‑1 (MQ=255)

                                                                                                                    agtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCATGACGTTGGTTAGAACGTTTTAACGCATCAAAAGTTTCTAATCGtt            >  1:10434890/1‑128 (MQ=255)

                                                                                                                      taAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                               >  1:9462765/1‑91 (MQ=255)

                                                                                                                       aaaTCTAAGACCATCTAATTGAATGATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTta         <  1:288691/128‑1 (MQ=255)

                                                                                                                       aaaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTta         >  2:630031/1‑128 (MQ=255)

                                                                                                                        aaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaa        >  1:8722576/1‑128 (MQ=255)

                                                                                                                         aTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGCACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaat       >  2:3135209/1‑128 (MQ=255)

                                                                                                                          tCTAACACCATCTACTTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaata      <  2:2150771/128‑1 (MQ=255)

TGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAATTT  >  CP016816/478612‑478865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`