Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 516,356 G→A intergenic (+187/+319) JCVSYN2_02375 → / ← JCVSYN2_02380 antitermination protein NusG/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816516,3560GA99.7% 17462.2 / NA 5427intergenic (+187/+319)JCVSYN2_02375/JCVSYN2_02380antitermination protein NusG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (2443/2959);  minor base T (5/10);  total (2451/2976)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5644 total aligned reads displayed.

AATAAAAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTA  >  CP016816/516232‑516477
                                                                                                                            |                                                                                                                         
 ataaaAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATaaaaaa                                                                                                                       <  2:8640763/128‑1 (MQ=255)
  taaaAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAaaaaaa                                                                                                                      <  2:1195816/128‑1 (MQ=255)
   aaaaaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAaaaaaac                                                                                                                     <  1:157805/128‑2 (MQ=255)
    aaaaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAATATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAaaaaaaaa                                                                                                                    >  2:5969977/1‑126 (MQ=255)
      aaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAtt                                                                                                                  >  1:4165471/1‑128 (MQ=255)
      aaCATAAAATATAATTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAATGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAtt                                                                                                                  <  1:8348056/128‑1 (MQ=255)
         aTAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTa                                                                                                               >  1:3216426/1‑128 (MQ=255)
             aaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAt                                                                                                           >  2:1397121/1‑128 (MQ=255)
              atatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAtt                                                                                                          >  2:9856501/1‑128 (MQ=255)
              atatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAtt                                                                                                          <  2:8207513/128‑1 (MQ=255)
               tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                         <  1:10003052/128‑1 (MQ=255)
               tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                         <  1:9548579/128‑1 (MQ=255)
               tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                         <  1:6757726/128‑1 (MQ=255)
                atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                        >  1:5066715/1‑128 (MQ=255)
                atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                        <  2:9481788/128‑1 (MQ=255)
                atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                        >  2:3394354/1‑128 (MQ=255)
                  atTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCa                                                                                                      >  1:3576653/1‑128 (MQ=255)
                    ttttTAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAt                                                                                                    >  1:2830166/1‑128 (MQ=255)
                    ttttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAt                                                                                                    <  1:597283/128‑1 (MQ=255)
                    ttttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTAATTAAAATTTTGTTTTTATTTTTAATTAAAATTATAATAAAAAAACATTTTAGTATTTGCAAt                                                                                                    <  2:7155167/128‑1 (MQ=255)
                     tttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAtt                                                                                                   <  1:1881341/128‑1 (MQ=255)
                      ttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATta                                                                                                  <  1:9891900/128‑1 (MQ=255)
                        aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtata                                                                                                >  2:7714917/1‑128 (MQ=255)
                        aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATATGAAATtata                                                                                                >  1:7497997/1‑128 (MQ=255)
                          aaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaa                                                                                              >  1:10433901/1‑128 (MQ=255)
                           aaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaaa                                                                                             >  2:5831337/1‑128 (MQ=255)
                           aaaTATGTTTTTATAAGATTAGAAAAAAAGCGTATATTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATCATaaac                                                                                             <  1:6552752/128‑2 (MQ=255)
                            aaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaaaa                                                                                            >  2:2742581/1‑128 (MQ=255)
                             atatGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaaaaa                                                                                           <  2:7376784/128‑1 (MQ=255)
                              tatGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGAAATTATAAAAAAg                                                                                          >  1:10246969/1‑128 (MQ=255)
                               atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGTCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                         <  2:3161904/128‑1 (MQ=255)
                               atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                         <  1:5592733/128‑1 (MQ=255)
                               atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                         <  2:9224007/128‑1 (MQ=255)
                               atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                         <  1:9356296/128‑1 (MQ=255)
                                 gTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGttt                                                                                       <  1:8137422/128‑1 (MQ=255)
                                  tttttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTa                                                                                      <  2:5470293/128‑1 (MQ=255)
                                  tttttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTa                                                                                      <  1:7116198/128‑1 (MQ=255)
                                    tttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATg                                                                                    >  1:2222893/1‑128 (MQ=255)
                                     ttATAAGATTAGAAAAAAAGCTTATTTTATGGGCTTTTTTAAAGATTATTTATATTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTATTATTTGCAATTTTAAAAAAGTTTATGa                                                                                   >  1:5417395/1‑128 (MQ=255)
                                     ttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGAAATTATAAAAAAGTTTATGa                                                                                   >  2:4344719/1‑128 (MQ=255)
                                        taAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCa                                                                                                      <  2:3903969/106‑1 (MQ=255)
                                        taAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataa                                                                                >  1:9144348/1‑128 (MQ=255)
                                        taAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataa                                                                                >  2:7866386/1‑128 (MQ=255)
                                          aGATTAGAAAAAAAGCTTATTTTAATGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                              <  2:493840/128‑1 (MQ=255)
                                          aGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                              <  2:10258914/128‑1 (MQ=255)
                                            aTTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAAtata                                                                            >  2:6321211/1‑128 (MQ=255)
                                             ttAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                           <  2:755685/128‑1 (MQ=255)
                                                 aacaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTAt                                                                       <  1:4691554/125‑1 (MQ=255)
                                                    aaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                    <  1:727131/128‑1 (MQ=255)
                                                      aaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAata                                                                  >  2:612464/1‑128 (MQ=255)
                                                      aaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAata                                                                  <  1:2455033/128‑1 (MQ=255)
                                                       aGCTTATTTTAATGTCTTTTTTAAAGATTATTTAAATTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAatat                                                                 <  2:1564456/128‑1 (MQ=255)
                                                       aGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAatat                                                                 >  2:8439275/1‑128 (MQ=255)
                                                       aGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAatat                                                                 >  2:454909/1‑128 (MQ=255)
                                                        gCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataa                                                                                <  1:2627118/112‑1 (MQ=255)
                                                         cTTTTTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAg                                                               <  2:6513390/128‑1 (MQ=255)
                                                          ttATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGa                                                              <  2:2044553/128‑1 (MQ=255)
                                                          ttATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACATATTAATATAGa                                                              >  2:5302694/1‑128 (MQ=255)
                                                            aTTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                            <  1:3030164/128‑1 (MQ=255)
                                                            aTTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                            <  1:1719364/128‑1 (MQ=255)
                                                             taaaataagCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATTATATACCTATTAATATAGAATa                                                           >  1:2057138/9‑128 (MQ=255)
                                                              tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                          <  2:4151732/128‑1 (MQ=255)
                                                              tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGATTATGATAATATACCTATTAATATAGAATaa                                                          >  2:8847646/1‑128 (MQ=255)
                                                                tAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaac                                                        <  1:873166/128‑1 (MQ=255)
                                                                    ggCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACt                                                    >  2:1870880/1‑128 (MQ=255)
                                                                      cTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAg                                                  <  1:7940034/128‑1 (MQ=255)
                                                                       ttttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGa                                                 >  1:4917308/1‑128 (MQ=255)
                                                                        tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                <  1:6188291/128‑1 (MQ=255)
                                                                         ttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaa                                               >  1:8542955/1‑128 (MQ=255)
                                                                         ttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaa                                               <  2:2219483/128‑1 (MQ=255)
                                                                         ttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaa                                               >  2:9658485/1‑128 (MQ=255)
                                                                          tttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaaa                                              <  2:170587/128‑1 (MQ=255)
                                                                          tttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaaa                                              >  1:6351948/1‑128 (MQ=255)
                                                                           ttAAAGATTATTTAATATTTTGTTTTTATTTTTAATTATAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAATATAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAag                                             <  2:2947609/128‑1 (MQ=255)
                                                                            tAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAaga                                            <  2:5635852/128‑1 (MQ=255)
                                                                             aaaGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAagag                                           <  2:5106779/128‑1 (MQ=255)
                                                                              aaGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAAt                                                                                                    <  2:6745190/70‑1 (MQ=39)
                                                                               aGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGaa                                         >  1:449385/1‑128 (MQ=255)
                                                                                gATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGaaa                                        >  1:3753805/1‑128 (MQ=255)
                                                                                  ttattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAATTTTATGATAATATACATATTAATATAGAATAAAAAACTAGAAAAGAGAAata                                      >  1:9690593/1‑128 (MQ=255)
                                                                                   tattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAatat                                     >  2:2414330/1‑128 (MQ=255)
                                                                                    attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                    >  1:3970891/1‑128 (MQ=255)
                                                                                     tttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatta                                   <  2:6949553/128‑1 (MQ=255)
                                                                                       tAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                 <  2:2570105/128‑1 (MQ=255)
                                                                                       tAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                 <  1:1371233/128‑1 (MQ=255)
                                                                                        aaaaTTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATAttatta                                >  2:10449198/1‑128 (MQ=255)
                                                                                            ttttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                         >  1:6924622/1‑67 (MQ=255)
                                                                                            ttttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTAt                            >  2:8030419/1‑128 (MQ=255)
                                                                                              ttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTTTGa                          >  1:5779709/1‑128 (MQ=255)
                                                                                               tGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGAt                         >  2:3726081/1‑128 (MQ=255)
                                                                                               tGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGAt                         >  1:5243586/1‑128 (MQ=255)
                                                                                               tGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGAt                         >  1:7708261/1‑128 (MQ=255)
                                                                                                  ttttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGc                      <  1:11799/128‑1 (MQ=255)
                                                                                                   tttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGATATATTATTAGTATGATAGCg                     >  2:3551750/1‑128 (MQ=255)
                                                                                                     tatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAATAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGAGaa                   >  2:4740530/1‑128 (MQ=255)
                                                                                                        ttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaatta                <  1:8930285/128‑1 (MQ=255)
                                                                                                              ttatAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttttt          <  1:4349520/128‑1 (MQ=255)
                                                                                                                aaaaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaa        <  2:9044363/128‑1 (MQ=255)
                                                                                                                 aaaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaa       >  1:3348300/1‑128 (MQ=255)
                                                                                                                  aaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaaa      >  2:10078079/1‑128 (MQ=255)
                                                                                                                            |                                                                                                                         
AATAAAAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTA  >  CP016816/516232‑516477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: