Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 22,024 G→A E537K (GAG→AAG)  JCVSYN2_00070 → tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681622,0240GA99.1% 9581.1 / NA 3398E537K (GAG→AAG) JCVSYN2_00070tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base G (10/11);  major base A (1801/1515);  minor base T (17/14);  total (1843/1555)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3410 total aligned reads displayed.

CGGATTGTAAAATCTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAGAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAGGTCATTCTTAGT  >  CP016816/21899‑22149
                                                                                                                             |                                                                                                                             
 ggATTGTAAAATCTGTTTTAAGTATGGCGTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGc                                                                                                                            <  1:7824254/128‑1 (MQ=255)
 ggATGGTAAAATCTGTTTTAAGGATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGc                                                                                                                            <  2:6462855/128‑1 (MQ=255)
  gATTGTAAAATCTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGcc                                                                                                                           >  1:5364711/1‑128 (MQ=255)
   aTTGTAAAATCTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAATTCTTAAAAAAAGCTGAGACAGAATTGTTAAAGCCa                                                                                                                          >  2:6993747/1‑128 (MQ=255)
   aTTGTAAAATCTGTTTTAAGTATGGCTAATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCa                                                                                                                          >  1:4265112/1‑128 (MQ=255)
    ttGTAAAATCTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCat                                                                                                                         >  1:6258884/1‑128 (MQ=255)
      gTAACATCTGGATTAAGTATTGATTATACTATAGCCCTGTTCGTACCCCAGCAGATTATCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCatat                                                                                                                       <  1:1695329/128‑1 (MQ=255)
      gTAAAATCTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCatat                                                                                                                       >  1:8983555/1‑128 (MQ=255)
      gTAAAATCTGTTTTAAGTATGGCTTATACTATAGCCCTGTGAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTAGTGTATTGGATCAAGTCTTAAAAAAATCTGGAAGAGAATTGTTAATGCGatat                                                                                                                       >  2:919296/1‑128 (MQ=255)
       tAAAATCTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATc                                                                                                                      <  2:10018789/128‑1 (MQ=255)
             cTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAATAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGtt                                                                                                                >  2:3060177/1‑128 (MQ=255)
             cTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTTGtt                                                                                                                >  1:5133974/1‑128 (MQ=255)
             cTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGtt                                                                                                                >  1:8383269/1‑128 (MQ=255)
             cTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTAAGtt                                                                                                                >  1:2714930/1‑128 (MQ=255)
              tGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGCATTGTTAAAGCCATATCTTAGttt                                                                                                               >  2:5024029/1‑128 (MQ=255)
              tGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGttt                                                                                                               <  1:61100/128‑1 (MQ=255)
                ttttAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTa                                                                                                             >  1:4965108/1‑128 (MQ=255)
                ttatAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGATAAAGCCATATCTTAGTTTTa                                                                                                             <  1:7215102/125‑1 (MQ=255)
                   tAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAATCCAGATCTTAGTTTTaaaa                                                                                                          >  1:2523324/1‑128 (MQ=255)
                         tGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATg                                                                                                    <  1:9596942/128‑1 (MQ=255)
                         tGGCTTATACTATAGCCCTGTTAGAACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATg                                                                                                    <  2:9567171/128‑1 (MQ=255)
                         tGGCTAATACTATAGCCCTGTAAGTACCACATCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATg                                                                                                    <  2:6647217/128‑1 (MQ=255)
                         tGGCATATACTATAGACCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATg                                                                                                    <  1:9780815/128‑1 (MQ=255)
                           gCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTAGTGGAACAAGTCTTAAAAAAAGCTGGAACAGATTTGTTAAAGCCAAATCTTAGTTTTAAAATTTATgcg                                                                                                  >  1:1869739/1‑128 (MQ=255)
                            cTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGTTGATTGCTACTATTGTATTGTAAAAAGTCTGAAAAAAATATGGAACATAATTGTTAATGCCAGTGCTTAGTTTTATAAATTAttagc                                                                                                 >  1:5705382/1‑124 (MQ=255)
                             ttATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTTAAGCCATATCTTAGATTTAAATTTTATGCGcc                                                                                                >  1:2852187/1‑128 (MQ=255)
                             ttATACTATAGCCCTGTTAGGACCCCAGGAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGcc                                                                                                <  2:7326949/128‑1 (MQ=255)
                              tatcCTATAGCCTAGGTAGAACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGATCAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCca                                                                                               <  2:5421457/128‑1 (MQ=255)
                               cttcTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGCACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCcac                                                                                              <  1:10001116/125‑1 (MQ=255)
                               ataCTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCcac                                                                                              >  2:10338949/1‑128 (MQ=255)
                                taCTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGTACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCcaca                                                                                             <  1:1120698/128‑1 (MQ=255)
                                taCTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCcaca                                                                                             <  1:3102174/128‑1 (MQ=255)
                                taCTATAGCCCTGCTAGTTCCACAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGACCAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCcaca                                                                                             <  1:1330581/128‑1 (MQ=255)
                                     taGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGTGCCACAGGAat                                                                                        <  2:1759434/128‑1 (MQ=255)
                                     taGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAat                                                                                        >  1:8012188/1‑128 (MQ=255)
                                         ccTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTACTGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCt                                                                                    >  2:4603097/1‑128 (MQ=255)
                                           tGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCttt                                                                                  >  2:9030453/1‑128 (MQ=255)
                                                 tACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAg                                                                            >  2:5567673/1‑128 (MQ=255)
                                                 tACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAg                                                                            >  2:575083/1‑128 (MQ=255)
                                                 tACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAg                                                                            >  1:8184592/1‑128 (MQ=255)
                                                  aCCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGc                                                                           <  1:6469474/128‑1 (MQ=255)
                                                   ccccAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCa                                                                          >  2:7892022/1‑128 (MQ=255)
                                                    cccAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCAt                                                                         <  2:3277017/128‑1 (MQ=255)
                                                     ccAGCAGATTTTCTGATGCATGCTCCTATTGTATTGGAACTAGTCTTAAACAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATa                                                                        <  2:517739/128‑1 (MQ=255)
                                                     ccAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATa                                                                        >  2:3803823/1‑128 (MQ=255)
                                                      cagcagATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATAc                                                                       <  2:7689013/128‑1 (MQ=255)
                                                      cagcagATTTTCGGATGCTTGCTCCTAATGTATTGCAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCATTCACGAGCAtcg                                                                       <  2:5970106/128‑3 (MQ=255)
                                                        gcagATTTTCGGATGCTTGATCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACATCAATATCTTTCACGAGCATACaa                                                                     >  1:5916997/1‑128 (MQ=255)
                                                          agATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGATTTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACg                                                                   >  2:3478784/1‑128 (MQ=255)
                                                            aTTTTCGGATGCTTGCTACTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGAt                                                                 <  2:1419740/128‑1 (MQ=255)
                                                               ttCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACgctgct                                                              >  2:2256475/1‑128 (MQ=255)
                                                                  ggATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGc                                                               <  1:7411284/124‑1 (MQ=255)
                                                                     tgcttgctCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTaaa                                                        >  2:10194570/1‑128 (MQ=255)
                                                                      gcttgctCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAat                                                       >  2:10500867/1‑128 (MQ=255)
                                                                         tgctTCTATTGATATGGAACAAGTATTAACAAAAGCTTGAACAGAATTGTTAAAGCCATATCTTAGATTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATAtt                                                    <  1:4705825/128‑1 (MQ=255)
                                                                         tgctCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATAtt                                                    <  2:1247014/128‑1 (MQ=255)
                                                                         tgctCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATAtt                                                    <  2:1575937/128‑1 (MQ=255)
                                                                          gctCCGATTGTATTGGAACAAGTCTTAAAACAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTTAAATTTATGCGCCACAGGAATATCTTTCACGACCATACAACTATGCTCCGAAATATtg                                                   >  1:8627415/1‑128 (MQ=255)
                                                                           ctCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATtgt                                                  >  2:9823223/1‑128 (MQ=255)
                                                                            tCCTATTGTAGTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATTCTACGATGCTGCTAAATATtgtg                                                 >  2:4824622/1‑128 (MQ=255)
                                                                                attttattgGAACAAGTCTTAAAAAAAGCTGGAACATAATTTTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCAACAGGAATATATTTCACGAGCATACAACGATGATCCTAAATATTTTGCGaa                                             >  1:8852779/1‑128 (MQ=255)
                                                                                 ttgtattgGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATtg                                                   >  1:1503265/1‑121 (MQ=255)
                                                                                    tattgGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATc                                         >  1:3877058/1‑128 (MQ=255)
                                                                                      ttgGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTaaa                                                        >  1:1991757/1‑111 (MQ=255)
                                                                                       tgGAACAAGGCTTAAAAAACGCTGGAACAGAATTGTTAAAGCCATATATTATTTTTAAAATTTATGCGGCACTGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTa                                      <  1:4476302/128‑1 (MQ=255)
                                                                                        ggcaCAAGTCGAAACACAAGCTGGACCAGACTTGTGAAAGCCATATCTTAGTTTTAAAATTAATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAg                                     <  1:6697652/125‑1 (MQ=255)
                                                                                        ggAACTAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAg                                     <  1:10152608/128‑1 (MQ=255)
                                                                                         gAACCAGACTAATAAGAAGCGGGAACAGAATTGTTACTGCCATATCTAAGTTTTAAAATTTATGCGCCACAGGAATAGCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGa                                    <  1:4041267/128‑1 (MQ=255)
                                                                                          aaCAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGac                                   >  2:678775/1‑128 (MQ=255)
                                                                                          aaCAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCGTAGGTTTAAATTTTATGCTCCACAGGAATATATTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGTAGCTCGTAGac                                   >  1:6867171/1‑128 (MQ=255)
                                                                                           tcAAGTCTTAAAAAAAGTTGGAAAAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGTACATCGTAGaca                                  <  1:3567844/127‑1 (MQ=255)
                                                                                             aaGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTGTAAAATTTATGCGCCACAGGAATATCTTTCCCGAGCATACAACGCTCCTCCTAAATATTCTGCGAACATCGTAGACACt                                >  1:5522824/1‑128 (MQ=255)
                                                                                              aGTCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTc                               <  2:1945839/128‑1 (MQ=255)
                                                                                                tCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCaa                             >  2:6301001/1‑128 (MQ=255)
                                                                                                tCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATAGTGCGAACATCGTAGACACTCaa                             >  2:4204663/1‑128 (MQ=255)
                                                                                                tCTTAAAAAAAGCTGGAACAGAATTGTTAAAGCCAAATCTTAGTATTAAAATTAATGCGACACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGAGCGATCATCGTAGACACTCaa                             >  2:7160708/1‑128 (MQ=255)
                                                                                                      aaaaaGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTATACACTCAATTGaaa                       >  2:6117344/1‑128 (MQ=255)
                                                                                                      aaaaaGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGaaaa                      >  2:2461021/1‑128 (MQ=255)
                                                                                                       aaaaGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCCCGAGCATACAACGCTGCTCCTAAATATTGTTCGAACATCGTAGACACTCAATTGcaaa                      >  1:9381457/1‑128 (MQ=255)
                                                                                                       aaaaGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGCCACTCAATTGaaaa                      >  2:7493948/1‑128 (MQ=255)
                                                                                                       aaaaGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGaaaa                      >  2:2655783/1‑128 (MQ=255)
                                                                                                       aaaaGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGaaaa                      >  1:7041893/1‑128 (MQ=255)
                                                                                                       aaaaGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGaaaa                      >  1:3360497/1‑128 (MQ=255)
                                                                                                       aaaaGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACGCAATTGaaaa                      >  1:2139719/1‑128 (MQ=255)
                                                                                                        aaaGCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGaaaaa                     >  2:864865/1‑128 (MQ=255)
                                                                                                           gCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAaataa                  >  1:2339409/1‑128 (MQ=255)
                                                                                                           gCTGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAaataa                  <  2:2108773/128‑1 (MQ=255)
                                                                                                             tGGAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATg                <  2:935311/128‑1 (MQ=255)
                                                                                                              ggAACCGACTTGGTAAAGCCTTAGCTTAGGTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGa               <  2:807791/128‑1 (MQ=255)
                                                                                                               gAACAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAACTAATGAg              >  2:8840146/1‑128 (MQ=255)
                                                                                                                aaCAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAgg             <  1:9198424/128‑1 (MQ=255)
                                                                                                                 aCAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAGGt            >  2:8387981/1‑128 (MQ=255)
                                                                                                                  cAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGATGTc           >  2:3665049/1‑128 (MQ=255)
                                                                                                                  cAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAGGTc           <  2:3973358/128‑1 (MQ=255)
                                                                                                                  cAGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAGGTc           >  2:4420480/1‑128 (MQ=255)
                                                                                                                   aGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAaataat                 <  2:2848735/121‑1 (MQ=255)
                                                                                                                   aGAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAGGTCa          <  1:10343228/128‑1 (MQ=255)
                                                                                                                    gAATTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAAGTGAAAAATAATGAGGTCag         >  2:8230024/1‑127 (MQ=255)
                                                                                                                     aaTTGTTAAAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAGGTCAtt        <  1:3718952/128‑1 (MQ=255)
                                                                                                                       ttGGTAAAGCCATATCTTAGTATTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAGGTCATTCt      <  1:7649033/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                             
CGGATTGTAAAATCTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAGAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTCACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAGGTCATTCTTAGT  >  CP016816/21899‑22149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: