BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	32,707	A→T	I1067F (ATT→TTT)	JCVSYN2_00105 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	32,707	0	A	T	99.5%	14369.8 / NA	4515	I1067F (ATT→TTT)	JCVSYN2_00105	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (18/7); new base T (2368/2115); total (2389/2126)
Fisher's exact test for biased strand distribution p-value = 6.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4677 total aligned reads displayed.

TGTTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGA  >  CP016816/32580‑32831

  ttACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCAtttttt                                                                                                                            <  1:2696418/128‑1 (MQ=255)

  ttACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGAGAAAGTGCAAACTACAGGGGAGTGAAAAGAAAAGATCTTAAACTTTATTTTAGGTGATTCTCCTTCAtttttt                                                                                                                            <  2:3436720/128‑1 (MQ=255)

  ttACTCAACCGTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAAGTACAGGTGAGTGAAAAGAAAAGATCTTACACTTAATTTTAGGTTATTCTCCGTCAtttttt                                                                                                                            >  1:1850600/1‑128 (MQ=255)

      tCAACCTTTAACTAAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAAt                                                                                                                        <  1:2887022/128‑1 (MQ=255)

        taCCTTTAAATCATTTTGTTGGAGAACTTTTAAAAAGTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCt                                                                                                                      <  1:2273396/127‑1 (MQ=255)

        aaCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCt                                                                                                                      >  2:8938723/1‑128 (MQ=255)

         aCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAGACTATTTTTAAAGATAAAGTGCAAACTACAGATGAGTGAAAAGAAAAGATCTTAAACTTAATTTTATTTTATTCTACTTCATTTTATAAATAtt                                                                                                                     >  1:3872861/1‑128 (MQ=255)

         aCCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTAAATTTTAGATTATTATCCTTCATTTTTTAAATCtt                                                                                                                     >  2:6701279/1‑128 (MQ=255)

          ccTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAACTCTTa                                                                                                                    >  2:6392639/1‑128 (MQ=255)

          ccTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTa                                                                                                                    <  1:9961/128‑1 (MQ=255)

           cTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAG‑AAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAtt                                                                                                                  <  2:8131997/128‑1 (MQ=255)

              tAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTa                                                                                                                >  2:2855385/1‑128 (MQ=255)

              tAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTa                                                                                                                >  1:5807085/1‑128 (MQ=255)

               aaCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTat                                                                                                               >  2:7983459/1‑127 (MQ=255)

               aaCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTaa                                                                                                               <  1:112677/128‑1 (MQ=255)

                aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                              <  2:5625974/128‑1 (MQ=255)

                aCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAAc                                                                                                              <  1:6708033/128‑1 (MQ=255)

                   cAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAg                                                                                                           <  2:3608981/128‑1 (MQ=255)

                      tttGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGa                                                                                                        >  2:7045719/1‑128 (MQ=255)

                        tgttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAt                                                                                                      <  1:3507023/128‑1 (MQ=255)

                         gttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATATTTTAAATCTTATTTAACAAGTGAAtc                                                                                                     >  2:8742064/1‑128 (MQ=255)

                          ttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtct                                                                                                    >  1:10204119/1‑128 (MQ=255)

                          ttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGCTTATTCTCCTTCATTTTTTAAATCTTATTTAACAATTGAAtct                                                                                                    >  2:6203770/1‑128 (MQ=255)

                           tgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTACTTCATTTTTTAAATCTTATTTAACAAGTGAAtctc                                                                                                   >  2:1206508/1‑128 (MQ=255)

                             gagcACTTTTAAACACTATTTTTAAAGATAAAGTGAAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                 <  2:1937517/128‑1 (MQ=255)

                             gagaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCt                                                                                                                      <  2:1017805/107‑1 (MQ=255)

                             gagaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                 >  1:8986651/1‑128 (MQ=255)

                              agaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGaa                                                                                                >  1:4500920/1‑128 (MQ=255)

                               gaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                               <  2:2319176/128‑1 (MQ=255)

                               gaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                               >  1:7102263/1‑128 (MQ=255)

                                 aCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAagag                                                                                             <  1:7472039/128‑1 (MQ=255)

                                    tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                          >  1:5634385/1‑128 (MQ=255)

                                    tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTc                                                                                          >  1:8603850/1‑128 (MQ=255)

                                     ctAAAAAGTCTTTTTAAAGATAAAGTGCAAACTACCGGTGAGTGAAAAGAAAAGATCTTAAACTTCATTTTAGGGTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCa                                                                                         <  2:5254651/127‑1 (MQ=255)

                                       aaaaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGCGCTCAAt                                                                                       >  2:9834176/1‑128 (MQ=255)

                                       aaaaaCTATTTTTAAAGATAAAGAGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTATAGGTTATTCTCATTCTTTTTTTAAATCTTATTTAACAACAGAATCTCGAAGAGCTCAAt                                                                                       >  2:3790409/1‑128 (MQ=255)

                                        aaaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAtt                                                                                      >  1:9753474/1‑128 (MQ=255)

                                           aCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTAATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTc                                                                                   >  2:10359787/1‑128 (MQ=255)

                                            cTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCt                                                                                  <  2:2648707/128‑1 (MQ=255)

                                             tATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtt                                                                                 >  2:10500474/1‑128 (MQ=255)

                                              aTTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCttt                                                                                >  2:9089127/1‑128 (MQ=255)

                                              aTTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCttt                                                                                >  2:4897762/1‑128 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             <  2:4314254/128‑1 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             >  2:5077365/1‑128 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             >  2:7620417/1‑128 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             <  1:1676437/128‑1 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGAGAGTGAAAAGAAAAGATCTTATACTTAATATTAGGTTTTTCTCCTTCATTTTTTAAATCTTCTTTAAGAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             <  2:6531120/128‑1 (MQ=255)

                                                   tAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGGTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGCATCTCGAAGAGCTCAATTTTCTTTTGGtt                                                                           >  2:2085929/1‑128 (MQ=255)

                                                        aTAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCGCAATTTTCTTTTGGTTGACaa                                                                      <  1:8059667/128‑1 (MQ=255)

                                                          aaaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaa                                                                    <  1:1302334/128‑1 (MQ=255)

                                                           taGTGCAATCTATATTGGAGTGAAAAGAATAGATATTTAACTTATTTTTAGGTTATTATTCTTCTTTTTTTAAATTTTATTTAACAAGTGAATCTCGTAGAGCTCAATTTTCTTTTGGTTGACaaaaa                                                                   <  1:8251949/127‑1 (MQ=255)

                                                           aaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaaa                                                                   <  2:5456997/128‑1 (MQ=255)

                                                            aGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaac                                                                  >  2:1385659/1‑128 (MQ=255)

                                                                cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaacaaac                                                              >  1:6252789/1‑128 (MQ=255)

                                                                cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaacaaac                                                              >  1:4863572/1‑128 (MQ=255)

                                                                cAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAaaacaaac                                                              >  2:1576943/1‑128 (MQ=255)

                                                                  aaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACta                                                            <  1:3069689/128‑1 (MQ=255)

                                                                   aCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACtat                                                           <  2:4668903/128‑1 (MQ=255)

                                                                    cTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACtata                                                          >  2:447801/1‑128 (MQ=255)

                                                                      aCAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAAt                                                        >  1:2050654/1‑128 (MQ=255)

                                                                       cAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAAtt                                                       <  2:3964009/128‑1 (MQ=255)

                                                                         ggTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTcc                                                     <  1:3302530/128‑1 (MQ=255)

                                                                         ggTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTcc                                                     >  1:8434105/1‑128 (MQ=255)

                                                                            gagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCcaca                                                  >  2:7416521/1‑128 (MQ=255)

                                                                            gagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCcaca                                                  >  1:10403544/1‑128 (MQ=255)

                                                                             agtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACTAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaa                                                 >  1:8785140/1‑128 (MQ=255)

                                                                             agtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaa                                                 <  1:6484647/128‑1 (MQ=255)

                                                                              gtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaa                                                <  2:8532245/128‑1 (MQ=255)

                                                                               tgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaaa                                               >  1:3683947/1‑128 (MQ=255)

                                                                               tgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaaa                                               >  1:10007199/1‑128 (MQ=255)

                                                                                   aagaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGCAGAGCTCAATGTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAACAAGaa                                           >  1:9367348/1‑127 (MQ=255)

                                                                                   aagaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGAt                                           >  2:3225312/1‑128 (MQ=255)

                                                                                   aagaaaagaTCTTAAACTTAATTTTAGGTTATTCACCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGAt                                           >  1:1461958/1‑128 (MQ=255)

                                                                                      taaaatttattaaCTTAATTTTAGGTTTTTCTCCTTAATTTTTTAAATCTTATTTAACTAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCaa                                        <  1:4069318/117‑1 (MQ=255)

                                                                                        aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                      <  2:7766126/128‑1 (MQ=255)

                                                                                        aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                      >  1:6062544/1‑128 (MQ=255)

                                                                                         agaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTa                                     <  2:9625324/128‑1 (MQ=255)

                                                                                         agaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTa                                     <  2:3072903/128‑1 (MQ=255)

                                                                                             cTTAAACTTAATTTTAGGTTAATCTCAATCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCaa                                 <  1:7654954/128‑1 (MQ=255)

                                                                                              ttAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAAc                                >  1:7885296/1‑128 (MQ=255)

                                                                                                aaaCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATAAATAAGCAAcga                              >  1:9592809/1‑126 (MQ=255)

                                                                                                 caCTTAATTTTAGGTTATTCTCCTTCATTTTATAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAt                             <  1:2486527/127‑1 (MQ=255)

                                                                                                  aCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAtt                            >  2:4127609/1‑128 (MQ=255)

                                                                                                  aCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAtt                            >  2:9455029/1‑128 (MQ=255)

                                                                                                     tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                         <  2:5807085/128‑1 (MQ=255)

                                                                                                      aaTTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTa                        >  1:6911560/1‑128 (MQ=255)

                                                                                                         tttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAc                     <  2:9271709/128‑1 (MQ=255)

                                                                                                          ttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAcc                    <  1:7278243/128‑1 (MQ=255)

                                                                                                              tttattaTCCTTCATTTTTTAAATCTTATTTAACAAGGGAATCTAGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTaaa                <  1:5458331/127‑1 (MQ=255)

                                                                                                              gTTATTCTCCTTCATTTTATAAATCTTAATTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTTGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAATAGCAACTATTTATAAACCTaaa                >  2:7238805/1‑128 (MQ=255)

                                                                                                                tATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATc              <  1:9185343/128‑1 (MQ=255)

                                                                                                                    ctcCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAAt          <  2:6005774/128‑1 (MQ=255)

                                                                                                                    ctcCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAAt          <  2:2484549/128‑1 (MQ=255)

                                                                                                                     tcCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATa         <  1:5268134/128‑1 (MQ=255)

                                                                                                                      ccTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCACCTATTTTTAAACCTAAATCAAATaa        >  1:5074980/1‑128 (MQ=255)

                                                                                                                      ccTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATaa        >  2:6896387/1‑128 (MQ=255)

                                                                                                                         taaTTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGATGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt     <  1:4694503/126‑1 (MQ=255)

                                                                                                                         tCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt     <  1:4284794/128‑1 (MQ=255)

                                                                                                                            ttttttAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGa  >  2:8303766/1‑128 (MQ=255)

                                                                                                                            ttttttAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGa  <  2:1759217/128‑1 (MQ=255)

TGTTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGA  >  CP016816/32580‑32831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`