Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA99.0% 11173.8 / 69.8 3697intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (2424/1215);  minor base . (11/26);  total (2437/1260)
Fisher's exact test for biased strand distribution p-value = 1.01e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3793 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAA  >  CP016816/165097‑165348
                                                                                                                              |                                                                                                                                
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                >  1:9309212/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               <  2:10325317/128‑1 (MQ=255)
  ttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatt                                                                                                                              >  2:1983683/1‑128 (MQ=255)
  ttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatt                                                                                                                              <  2:10141370/128‑1 (MQ=255)
    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                            >  2:1415480/1‑128 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:6107900/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:4861850/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                        <  1:6426734/128‑1 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTaa                                                                                                                      >  1:3826283/1‑128 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                      >  2:775151/1‑128 (MQ=255)
          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                      <  1:3656642/128‑1 (MQ=255)
           ttactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAc                                                                                                                     >  2:1215758/1‑128 (MQ=255)
             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                                   >  2:6208238/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGt                                                                                                                 >  1:9477213/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:867295/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  1:5254373/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:8208705/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTCTATATTCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  1:5752770/1‑128 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                               <  1:7887082/128‑1 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTaaa                                                                                                              >  2:3124354/1‑128 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                              >  1:1973594/1‑128 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTAAAATTTTTAAAAAATTAATTTTAAATTTTaaaaaa                                                                                                           >  2:6741322/1‑128 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAATTGATCTTGTaaaaaa                                                                                                           >  2:4480338/1‑128 (MQ=255)
                     aGTTGATAATATTTAGTTTAATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                           <  2:7112884/128‑1 (MQ=255)
                      gTTGCTAATATTTAGTATCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaaa                                                                                                          <  2:979791/128‑2 (MQ=255)
                       ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                         >  2:8506146/1‑128 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                       >  2:1045882/1‑128 (MQ=255)
                           taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtt                                                                                                    >  1:142081/1‑128 (MQ=255)
                             atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGAtttt                                                                                                  >  2:1774894/1‑128 (MQ=255)
                             atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTAGAACTTGTAAAAAATAGCtttt                                                                                                  >  2:9653150/1‑128 (MQ=255)
                                tttAGTTTCATTAAATTAACCCACTTTTTATAATTACTAATAAACTAAAACTATTATCTTTTTATAAATTTTTTGTATAAGCAAAATTTTAAAAAAATAATTTTAACATGTAAAAAAAACCttttttt                                                                                              <  1:2650095/128‑1 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                            >  2:1570490/1‑128 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                            >  2:382448/1‑128 (MQ=255)
                                   aGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttt                                                                                           >  2:329979/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGatttttattttt                                                                                          >  2:2611863/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttattttt                                                                                          >  2:8068054/1‑128 (MQ=255)
                                       ttatttatttATCGCACTTTTATGAATTAGTAAGAAAATAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAAAAGCtttttttttttttt                                                                                       <  2:517027/126‑1 (MQ=255)
                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                       >  2:9491971/1‑128 (MQ=255)
                                           tAATTTAACGCAATTTTTTTAATTATTAATAAAAAAAAAAAATTTTATTTTTTATATTTTTTTTTTTATTCAAATTTTTAAAAAAATAATTTTAAATTGAAAAAAAAAGCtttttttttttttttttt                                                                                   <  1:3237410/128‑1 (MQ=255)
                                            aaTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttt                                                                                  >  1:8369650/1‑128 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAATAGCttttttttttttttttttttt                                                                                >  1:5408762/1‑126 (MQ=255)
                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtat                                                                                >  2:7321055/1‑128 (MQ=255)
                                                 aTCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTCTAAAAAATAGCttttttttttttttttatataatt                                                                             >  2:5667797/1‑120 (MQ=255)
                                                   ccccattGTATCTATTAGTAAGAAACAAAAAATATAATATATTATTTATTTTTTTTTAATTGAAAAATTTAAAAAAATTTATTTTTACTTGTAAAAAATACCGTTTTTTTTTTTTTTTTTTATAATgg                                                                           <  1:1244203/123‑1 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtaataata                                                                           >  2:8965009/1‑122 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttttaatataaatt                                                                       >  1:4117252/1‑117 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTAGAAAAAAATAGCttttttttttatttttttaaattaaaattt                                                                       >  1:2387282/1‑116 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATTTAAatat                                                                     >  1:7548803/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTGAAatat                                                                     >  2:8327537/1‑128 (MQ=255)
                                                         tttattaattattaatAAAATAAAACTATTATATTTTTATATATTTTTTTTATATTAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAAAATCTTTTTTTTTTTTTTTTTTTATAATGGAAatat                                                                     <  1:982814/126‑1 (MQ=255)
                                                          gTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATGGaataata                                                                    >  1:7330006/1‑123 (MQ=255)
                                                           tATTAATTAGTAATTAATTAAAAATTTTTTTTTTTTATTTATTTTTTTTTTATTTAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAAAAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                   <  2:7541935/128‑1 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtttataatttatttta                                                                   >  2:9345998/1‑116 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTTATTTAAAAATaa                                                                   >  1:10007984/1‑128 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATTTaa                                                                   >  2:8838753/1‑128 (MQ=255)
                                                            aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAAttaatataaatat                                                                 >  2:6564453/1‑116 (MQ=255)
                                                              gAATTATTAATAAAATAAAAATATTTTTTTTTTTTATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAttt                                                               <  2:4808112/128‑1 (MQ=255)
                                                               tattattattaaactaaaaattTTTTTTTTTTTTTTATTTTTTGTTTTTGCAAATTTTTAAAAAATTTATTTTAACTTTTAAAAAATTCGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtttt                                                              <  2:7916576/118‑1 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAGTTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAATTGTAATATAAATTTTattat                                                         >  1:5053098/1‑124 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAAATATATTAAATATAAATTTTAgg                                                           >  1:451867/1‑128 (MQ=255)
                                                                    gttaccataaaaaactaTTTTTGTTTTTGATATTTTTTGTATATTCAAAATTTTAAAAAATTTATTTTTACGTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                          <  1:6668064/118‑1 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTattt                                                          >  2:4665644/1‑125 (MQ=255)
                                                                     taataaAATAAAAATATTATTTTTTTATATATTTTTTGTATATTTAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                          <  2:3664969/128‑1 (MQ=255)
                                                                     tAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATGTAAAAATAAATTTTattt                                                          >  2:2136843/1‑125 (MQ=255)
                                                                      aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTAGTATAAAATAGCTTTTTTTATTTTTTTATTATAATCTAAATAAAAAttaaattaatt                                                       >  2:2315806/1‑119 (MQ=255)
                                                                       attaaataaaaattTTTTTTTTTTTGTTTTTTTTTTTTTATTTAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAATATGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAttt                                                      <  1:5546781/122‑1 (MQ=255)
                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAATTAAATAAAAATTTTTAGtttttttt                                                   >  1:10169656/1‑126 (MQ=255)
                                                                          aactaaaactaTAATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTatataatattt                                                   >  2:5866727/1‑118 (MQ=255)
                                                                              taaaataTTATCTTTTTTTTTTTTTTTTTTATTTTTAAATTTTTAAAAAATTAATTTTTAATTTTTTAAAAT‑‑‑TGATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                             <  1:7087232/127‑1 (MQ=255)
                                                                              aaaaattTTATCTTTTTATTTATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                                <  2:9134226/128‑1 (MQ=255)
                                                                                 actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATttttttttaaa                                             >  2:5006724/1‑123 (MQ=255)
                                                                                 aatattatTTTTTTATATATTTTTTTTATTTTTAAATTTTTAAAAAATTTATTTTTAATTTTTAAAAATTTCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                             <  2:6385166/126‑1 (MQ=255)
                                                                                  aaattatATTTTTTTATAATTTTTTTATAATAAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAATATGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                            <  2:5322778/126‑1 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAAATGAAAATAAAAATTTTATTTATtttttttaaaaat                                           >  2:3485579/1‑121 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATAAAAATTTTATGTATTTTTTTTTATaataat                                        >  1:3033852/1‑124 (MQ=255)
                                                                                       atCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATTTAAAAATAAATTTTAGTTATTTTTTTTTATAATTAta                                       >  1:8743580/1‑127 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTATAATTATATaa                                    >  2:5486006/1‑128 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTTTTTTAAAATTATATaaaaa                                 >  1:1744264/1‑128 (MQ=255)
                                                                                             ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTATTTTTTTTTTTTTTATAATTTAAAAAAAAATTATATTTATTTTTTTTTCTAATTATaaaaaa                                  >  1:8195657/1‑128 (MQ=255)
                                                                                              tttatatTTTTTTTATATTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                               <  2:29650/125‑1 (MQ=255)
                                                                                              tAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGTAAATATAAATTTTTGTTATTTTGTTTAATAAGTAGATaaaaaaa                               >  1:9122901/1‑128 (MQ=255)
                                                                                                 gaaaatttttGTATAAGCAAATTTTTAAAAAATTAATTTGAAAATTTAAAAAAATTCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAAt                              <  1:9622929/123‑1 (MQ=255)
                                                                                                 atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAATTTAAAAATAATTTTTATTTATTTTTTTTTATAATTAaaaaaaaaaaaa                             >  2:246327/1‑126 (MQ=255)
                                                                                                  attttttttttATAAGCAAATTTTTAAAAAATTAATTTTAACTTTTTAAAAAAAGTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAACTAAAAAAATa                             <  1:6853015/125‑1 (MQ=255)
                                                                                                   atTTTTTTTATATTTAAAATTTATAAAAATTAATTTTTATATTTTAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                           <  2:6906615/128‑1 (MQ=255)
                                                                                                   atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAAATata                           >  2:6041921/1‑126 (MQ=255)
                                                                                                       ttttttttATGAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaaa                         <  1:3462863/128‑1 (MQ=255)
                                                                                                         ttttttatGAAAATTTTTAAAAAATTATTTTTAACTTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaa                     <  1:1072304/123‑1 (MQ=255)
                                                                                                         ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTGTTTTATATTTATATAAAAAAATatatagta                      >  1:2858367/1‑121 (MQ=255)
                                                                                                           gttttttgaaaTTTTTAAAAAATTTATTTTAACTTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                    <  1:8533238/120‑1 (MQ=255)
                                                                                                            tttatttaaaTTTTTTAAAAATTAATTTTTAATTTTAAAAAATTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                   <  1:2198150/121‑1 (MQ=255)
                                                                                                            tttatttaaaTTTTTAAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                   <  1:1037795/121‑1 (MQ=255)
                                                                                                               ttttaaaTTTTTAAAAAATTTATTTTTTTTTTTTAAAAATATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                <  1:4694065/124‑1 (MQ=255)
                                                                                                               attcAAATTTTTAAAAAATTTATTTTTACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                <  1:9966008/125‑1 (MQ=255)
                                                                                                                  cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATGGAATTATTATTTTTTGGTTTTTTTTTTTCTATGTATCTAAAAAATAGAAAGTAAAAATTTTTg            >  2:4067853/1‑128 (MQ=255)
                                                                                                                   aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAATTGAAAATAAAATTTTTTGTTTTTTTTTTGTAAAATAAAAAAAAAAATTAAAAAGTAAAAAAAAtttt           >  1:894930/1‑126 (MQ=255)
                                                                                                                    aaTTTTTAAAAAATTAAGTTGAACTTGTAAAAAATAGCTGTTTTTTTTTTTTTTTTTTAAATGGAAATAAAAATTTTTGTTATTTTTTTTGATATTTTACAAAAAAAAAAAAAATTAAAAAATAttat           >  1:589489/1‑126 (MQ=255)
                                                                                                                     aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTGTCTAAGTATCAAAAAAAATATAAAGCAAAAAATTTTGtt          >  1:8060085/1‑128 (MQ=255)
                                                                                                                     aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAATTATAAATTTTAGTTTTTTTTTTTTATAATTATATAAAAAATTAAAAATGAAAAAATTTTGTTa         >  2:4294069/1‑128 (MQ=255)
                                                                                                                          taaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAATGTAAATTAAATTTTTAGTTATTTTTTTTTATATTTTTATAAAAAAAATAAAATAAAAAAATATTGTAaaaat     >  2:2778701/2‑127 (MQ=255)
                                                                                                                              |                                                                                                                                
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAA  >  CP016816/165097‑165348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: