BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	281,616	C→T	Q100* (CAA→TAA)	JCVSYN2_01185 →	DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	281,616	0	C	T	99.8%	16185.9 / NA	5092	Q100* (CAA→TAA)	JCVSYN2_01185	DNA‑binding protein
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (2481/2594); minor base A (9/3); total (2495/2597)
Fisher's exact test for biased strand distribution p-value = 8.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5125 total aligned reads displayed.

TATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281490‑281740

 aTGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATaa                                                                                                                             <  1:9066038/128‑1 (MQ=255)

 aTGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATaa                                                                                                                             <  2:6607446/128‑1 (MQ=255)

  tGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAaataat                                                                                                                            >  1:674857/1‑128 (MQ=255)

    attctaattctaAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAAttt                                                                                                                          <  1:2236544/128‑1 (MQ=255)

     ttCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTta                                                                                                                         <  1:4082099/128‑1 (MQ=255)

     ttCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTta                                                                                                                         <  2:5387978/128‑1 (MQ=255)

     ttCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTta                                                                                                                         <  1:9449710/128‑1 (MQ=255)

       cTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTtata                                                                                                                       <  1:541509/128‑1 (MQ=255)

       cTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTtata                                                                                                                       >  1:10393904/1‑128 (MQ=255)

        tATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTAACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaa                                                                                                                      <  2:436181/128‑1 (MQ=255)

           ttCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATaaaaa                                                                                                                   >  1:3229460/1‑128 (MQ=255)

            tCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAg                                                                                                                  <  2:2909120/128‑1 (MQ=255)

              tAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGtt                                                                                                                >  1:1789619/1‑128 (MQ=255)

               aaatCTAGTAAATTATTATTTAATTTAGAGAGAAAATTACATTTAAAACAAATACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGttt                                                                                                               <  1:3827792/128‑1 (MQ=255)

               aaaaCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGttt                                                                                                               <  2:6116775/128‑1 (MQ=255)

                  aCTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaa                                                                                                            <  1:5225199/128‑1 (MQ=255)

                   cTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaaa                                                                                                           >  1:2856596/1‑128 (MQ=255)

                   cTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTaaaa                                                                                                           >  1:4303731/1‑128 (MQ=255)

                    tAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATATAATTGAATCAAATAAAATAGATGAAAAATAATTTATAAAAACTTTAAAag                                                                                                          >  1:6917316/1‑128 (MQ=255)

                    tAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAag                                                                                                          <  1:8866382/128‑1 (MQ=255)

                      gTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                        <  2:7559790/128‑1 (MQ=255)

                      gTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                        <  2:4536633/128‑1 (MQ=255)

                      gTAAATTATTAATTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAagag                                                                                                        <  2:2029220/128‑1 (MQ=255)

                       tAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAgg                                                                                                       <  1:1452333/128‑1 (MQ=255)

                           ttattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAt                                                                                                   >  1:9661091/1‑128 (MQ=255)

                            tattattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAtt                                                                                                  >  2:8719023/1‑128 (MQ=255)

                             attattTAATTTAGAGACAAAATTACATTTAAAACAAAAACTGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAttt                                                                                                 <  1:6578640/128‑1 (MQ=255)

                              ttattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTTAAACAAATCAAATAGATGAAAAATAATTTATAAAAATTTTAAAATATATGATTtg                                                                                                >  2:10352048/1‑128 (MQ=255)

                               tattTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTtga                                                                                               <  1:6036667/128‑1 (MQ=255)

                                   taatttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAATAGGTGATTTGATGaa                                                                                           >  2:6989490/1‑128 (MQ=255)

                                     atttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAg                                                                                         <  2:1413848/128‑1 (MQ=255)

                                       ttaGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAATGTTTATAAGAGGTGATTTGATGAAAGTa                                                                                       >  2:8283258/1‑128 (MQ=255)

                                         agagACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTAtt                                                                                     >  1:9807421/1‑128 (MQ=255)

                                            gaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AAt                                                                                 <  2:1058861/128‑1 (MQ=255)

                                            gaCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AAt                                                                                 <  2:1770060/128‑1 (MQ=255)

                                             aCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAATTTTAAAAGAGGTGATATGATGAAAGTATA‑AATg                                                                                >  2:5756433/1‑128 (MQ=255)

                                             aCAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATg                                                                                >  2:10155615/1‑128 (MQ=255)

                                               aaaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGAt                                                                              <  1:7435243/128‑1 (MQ=255)

                                               aaaaATACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATTAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGAt                                                                              <  1:7257758/128‑1 (MQ=255)

                                                aaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATc                                                                             <  2:3285108/128‑1 (MQ=255)

                                                aaaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATc                                                                             >  2:892497/1‑128 (MQ=255)

                                                 aaTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCCAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCg                                                                            <  1:7069994/128‑1 (MQ=255)

                                                  aTTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCgg                                                                           >  2:982099/1‑128 (MQ=255)

                                                       aTTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATg                                                                      <  1:2396436/128‑1 (MQ=255)

                                                           aaaacataaacaTGATTTACTAATTTCTCTAATTAAAAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTa                                                                  <  1:2626823/128‑1 (MQ=255)

                                                            aaacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAATAGAGGTGATTTGATGAAAGTATT‑AATTATCGGAGATGTTTAt                                                                 >  1:5601237/1‑128 (MQ=255)

                                                             aacaaaaacaGTATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATg                                                                <  2:3494134/128‑1 (MQ=255)

                                                             aacaaaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATg                                                                >  2:4205467/1‑128 (MQ=255)

                                                                 aaaacaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCaaa                                                            <  2:4955559/128‑1 (MQ=255)

                                                                    acaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAAcc                                                         <  1:1282848/128‑1 (MQ=255)

                                                                    acaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAAcc                                                         <  1:9223607/128‑1 (MQ=255)

                                                                    acaGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAAcc                                                         >  2:1236302/1‑128 (MQ=255)

                                                                      aGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGATAAATAATGTATAAAAAGTTTAAAAGAGTTGATTTGATGAAAGTATT‑TATCATCGTAGATGTTTATGCTAAACCgg                                                       >  2:5582273/1‑128 (MQ=255)

                                                                      aGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCgg                                                       <  2:2375680/128‑1 (MQ=255)

                                                                       ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGTAGATGTTTATGCAAAACCggg                                                      >  2:3666200/1‑128 (MQ=255)

                                                                       ggATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGAGTTGATGAAAGTATT‑AATGATCGGAGATGTTTCTGCAAAACCggg                                                      >  1:8513722/1‑128 (MQ=255)

                                                                          tttACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAag                                                   >  1:10006217/1‑128 (MQ=255)

                                                                            tACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAagag                                                 <  2:6340564/128‑1 (MQ=255)

                                                                             aCTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAgaga                                                >  1:4914921/1‑128 (MQ=255)

                                                                              cTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGaa                                               <  2:5961786/128‑1 (MQ=255)

                                                                               tAATTTCTCTAATTAATAAAATTGAATCAAATAAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGATGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGaaa                                              <  2:9256915/128‑1 (MQ=255)

                                                                                aaTTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAAt                                             <  1:5840403/128‑1 (MQ=255)

                                                                                 aTTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAATACCGGGAAGAGAAATg                                            >  1:8727393/1‑128 (MQ=255)

                                                                                 aTTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATg                                            <  1:3460032/128‑1 (MQ=255)

                                                                                  tttCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCTGGAAGAGAAATGc                                           >  2:7746600/1‑128 (MQ=255)

                                                                                     ctctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAg                                        <  1:4498445/128‑1 (MQ=255)

                                                                                      tctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCTGGAAGAGAAATGCTAGa                                       >  2:2741007/1‑128 (MQ=255)

                                                                                      tctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGATGAGAAATGCTAgc                                       >  2:7190081/1‑127 (MQ=255)

                                                                                      tctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGa                                       >  2:9663182/1‑128 (MQ=255)

                                                                                       ctAATTAATAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaa                                      <  2:9079616/128‑1 (MQ=255)

                                                                                          attaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaaaa                                   <  2:4022407/128‑1 (MQ=255)

                                                                                          attaatAAAATTGAAACAAATCAAATAGATGAAAAATAATATATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGaaaaa                                   <  1:5077142/128‑1 (MQ=255)

                                                                                           ttaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAAc                                  <  1:1099507/128‑1 (MQ=255)

                                                                                           ttaatAAAATTGAAACAAATCAAATAGATGAAAAATAATTTAT‑AAAATTTTAAAAATAGTGAATTTAATAAAGTTTTAAATAAAAGATAATTTTTAT‑CAAAAACGGGAATAGAAATTCTATcaaaaac                                 >  2:9472735/1‑126 (MQ=255)

                                                                                            taataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACa                                 >  2:8063964/1‑128 (MQ=255)

                                                                                             aataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAt                                <  1:2011942/128‑1 (MQ=255)

                                                                                             aataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAt                                <  1:7794582/128‑1 (MQ=255)

                                                                                             aataaAATTGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGATAGAATAACAt                                >  1:7603050/1‑128 (MQ=255)

                                                                                               tataaTTGATACATATCAAATAGATGAAATATAATTTATAAAAAGTTTAAAAGAGGTTATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAAACGGGAAGAGAAATGCTAGAAAAACAttt                              <  1:4697396/125‑1 (MQ=255)

                                                                                                     tGAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTaaaaaa                        <  1:8143671/128‑1 (MQ=255)

                                                                                                      gAAACAACTCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAat                       <  1:6355710/128‑1 (MQ=255)

                                                                                                      gAAACAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAat                       >  2:3084008/1‑128 (MQ=255)

                                                                                                       aaaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAAATGGGAAGAGAAATGCTAGAAAAACATTTAAAAAata                      >  1:619026/1‑128 (MQ=255)

                                                                                                        aaCAAATCAAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAatat                     <  2:789731/128‑1 (MQ=255)

                                                                                                         aCAAATCGAATAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAATCATTTAAAAAATAtt                    >  1:7973334/1‑128 (MQ=255)

                                                                                                          caaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttg                   <  2:2204661/128‑1 (MQ=255)

                                                                                                           aaatcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATAttgt                  <  2:8486464/128‑1 (MQ=255)

                                                                                                              tcaaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACAGTTAAAAAATATTGTTGa               >  2:678020/1‑128 (MQ=255)

                                                                                                               caaatAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAACACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTTTTGat              >  1:3634687/1‑128 (MQ=255)

                                                                                                                  atAGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATGTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaa           <  2:2578996/128‑1 (MQ=255)

                                                                                                                    aGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaa         <  2:3872120/128‑1 (MQ=255)

                                                                                                                    aGATGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaa         <  2:1570522/128‑1 (MQ=255)

                                                                                                                      aTGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaatc       >  1:163734/1‑128 (MQ=255)

                                                                                                                       tGAAAAATAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTTatcaatatca      >  1:8364419/1‑128 (MQ=255)

                                                                                                                         aaaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGatcaaaaacaaa    >  1:9960579/1‑128 (MQ=255)

                                                                                                                          aaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATTCTAGAAAAACATTTAAATAATATTGTTGATCAAAATCAAAt   >  1:3049330/1‑128 (MQ=255)

                                                                                                                          aaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGAtcaaattcaaat   >  2:4741492/1‑128 (MQ=255)

                                                                                                                          aaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAAt   >  2:7375599/1‑128 (MQ=255)

                                                                                                                          aaaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAAt   <  1:10162681/128‑1 (MQ=255)

                                                                                                                           aaaTAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAACTGCTAGAAAAACATTTAAAAAATATTCTTGATCAAAATCAAATa  >  2:5164674/1‑128 (MQ=255)

TATGATTCTATTTCTAAAACTAGTAAATTATTATTTAATTTAGAGACAAAATTACATTTAAAACAAAAACAGGATTTACTAATTTCTCTAATTAATAAAATTGAAACAAATCAAATAGATGAAAAACAATTTATAAAAAGTTTAAAAGAGGTGATTTGATGAAAGTATT‑AATGATCGGAGATGTTTATGCAAAACCGGGAAGAGAAATGCTAGAAAAACATTTAAAAAATATTGTTGATCAAAATCAAATA  >  CP016816/281490‑281740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`