Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,157 2 bp→TT intergenic (‑440/+402) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1570AT99.3% 12895.7 / NA 4124intergenic (‑440/+403)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (17/11);  new base T (2213/1881);  total (2230/1894)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1580AT98.7% 12370.7 / ‑2.8 4124intergenic (‑441/+402)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (2/53);  new base T (2228/1841);  total (2230/1894)
Fisher's exact test for biased strand distribution p-value = 3.79e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4299 total aligned reads displayed.

TAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTAT  >  CP016816/420035‑420282
                                                                                                                          ||                                                                                                                              
taaattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatt                                                                                                                            >  1:1075685/1‑128 (MQ=255)
   attcattcATACTTTTATTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                         <  2:1515818/128‑4 (MQ=255)
       attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                     <  2:730214/128‑1 (MQ=255)
        ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                    <  1:7912756/128‑1 (MQ=255)
        ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                    >  1:3033336/1‑128 (MQ=255)
         tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                   >  1:10190987/1‑128 (MQ=255)
           atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                 >  2:6392488/1‑128 (MQ=255)
           atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                 <  2:5444251/128‑1 (MQ=255)
           atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                 <  2:2415991/128‑1 (MQ=255)
            tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                <  1:257496/128‑1 (MQ=255)
             aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                               >  2:479511/1‑128 (MQ=255)
              cTTTTAGTCTCATATATCTATGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                              <  1:10373059/128‑1 (MQ=255)
                  tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttt                                                                                                          <  1:7665119/128‑1 (MQ=255)
                   aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                         >  1:2278083/1‑128 (MQ=255)
                   aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                         <  1:4041906/128‑1 (MQ=255)
                     tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                       >  2:10078150/1‑128 (MQ=255)
                      ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATAACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCa                                                                                                      >  2:1733852/1‑128 (MQ=255)
                       tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                     >  2:1327094/1‑128 (MQ=255)
                       tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                     <  2:6189994/128‑1 (MQ=255)
                       tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                     <  1:8863746/128‑1 (MQ=255)
                         aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   <  2:7520889/128‑1 (MQ=255)
                         aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   <  2:8520567/128‑1 (MQ=255)
                         aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATATACTTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                  >  1:4477879/1‑128 (MQ=255)
                           aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTAATAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                 >  2:3681088/1‑128 (MQ=255)
                            aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                <  2:2854027/128‑1 (MQ=255)
                            aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                <  1:7106962/128‑1 (MQ=255)
                             aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                               <  1:6895416/128‑1 (MQ=255)
                             aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTATAAAAAATTTTTAATTATAATAACTGAATTATATTTCAAATTGtt                                                                                               >  2:5847207/1‑128 (MQ=255)
                                 aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                           <  1:1966284/128‑1 (MQ=255)
                                   gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                         <  2:1123203/128‑1 (MQ=255)
                                     ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                       >  1:1227572/1‑128 (MQ=255)
                                     ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                       >  1:6681647/1‑128 (MQ=255)
                                     ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTATCAAATTGTTAAattatt                                                                                       >  2:3506427/1‑128 (MQ=255)
                                      tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                      >  2:8888852/1‑128 (MQ=255)
                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCCCTTTTTTAAAAAATTTTTAATTTTAATAAATGAAATATTTTTCAAAATTTTAAATTAttta                                                                                     >  2:2559735/1‑127 (MQ=255)
                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTAtttt                                                                                     >  1:5717353/1‑128 (MQ=255)
                                        tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                    <  2:7870720/128‑1 (MQ=255)
                                          aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                  <  1:9376231/128‑1 (MQ=255)
                                           aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                 <  2:8156881/128‑1 (MQ=255)
                                                 tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                           <  1:3193918/128‑1 (MQ=255)
                                                  ttATCATTTATAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                          <  2:10226607/128‑1 (MQ=255)
                                                   tATCATTTAAAAAAATAATACTCTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                         <  1:9852110/128‑1 (MQ=255)
                                                    aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                        <  1:1718338/128‑1 (MQ=255)
                                                     tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                       >  2:4618371/1‑128 (MQ=255)
                                                     tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                       <  2:4083727/128‑1 (MQ=255)
                                                       aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTACAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                     >  1:9219163/1‑128 (MQ=255)
                                                           aaaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATc                                                                 >  2:10423065/1‑128 (MQ=255)
                                                            aaaaaaTAATACTTTTATTAGTTGATATATTAGAAAAATACTAGCACTTTTATAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa                                                                <  1:8545027/128‑1 (MQ=255)
                                                                aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                            <  2:6763967/128‑1 (MQ=255)
                                                                aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                            <  1:5519020/128‑1 (MQ=255)
                                                                aataatACTATTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                            <  2:9818037/128‑1 (MQ=255)
                                                                    aaaattttATTAGTTGATATAATAGAAAACTACTAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATg                                                        <  1:5307137/124‑1 (MQ=255)
                                                                     taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                       >  2:2266402/1‑128 (MQ=255)
                                                                       cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                         <  1:8737693/92‑1 (MQ=255)
                                                                        ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCg                                                    >  2:2089223/1‑128 (MQ=255)
                                                                        ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCg                                                    >  2:692835/1‑128 (MQ=255)
                                                                        ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGATct                                                    >  1:9562453/1‑127 (MQ=255)
                                                                          ttattaTTTGTTATATTACAAAAATAATATCATTTTTTTAAAAAATTTTTATTTTTAAAAAAATTATTATTTTTCAAATTTTTAAATTATTTTCAAATAATTATCTATTAATCAATTTATGCTCGttt                                                <  1:4744661/128‑1 (MQ=255)
                                                                           tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATc                                                                 >  2:8337778/1‑112 (MQ=255)
                                                                            attaGTTTTTAACTTAGTAAACTAATATAACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTTAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttt                                               <  2:9221609/128‑1 (MQ=255)
                                                                            attaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATg                                                        <  2:7112748/120‑1 (MQ=255)
                                                                              taGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTCGtttttt                                             >  2:9535366/1‑128 (MQ=255)
                                                                                tttGATATATTATAAAAATACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTTTTTTTAAAATTTTTAAATTATTTTCAAATCATTATATATTAATCAATTTTATGCTCGtttttttt                                           <  1:8222236/127‑1 (MQ=255)
                                                                                 ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttttttttt                                          >  2:8722573/1‑128 (MQ=255)
                                                                                   gATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAATTTTATTCTCGttttttttttt                                        >  2:7708832/1‑128 (MQ=255)
                                                                                    atatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTAGtttttttttttt                                       >  1:10002761/1‑128 (MQ=255)
                                                                                      ttattatAAAACTAATATCACTTTTTTAAAAAATTTTTAATTTTAATTACTGAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATGTTTTAATCAATTTTATGCTCGtttttttttttttt                                     <  1:3794900/127‑1 (MQ=255)
                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttttttttttt                                     >  1:9057152/1‑128 (MQ=255)
                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttttttttttt                                     >  2:6025900/1‑128 (MQ=255)
                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttttttttttt                                     >  2:938141/1‑128 (MQ=255)
                                                                                           aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtattttttttttttttaca                                >  2:6564499/1‑125 (MQ=255)
                                                                                             taaaaaactaGTACTTTTTTAAAAAATTTTTAATTTTAAAAACTTAATTTTTTTTCAAATTTTTAAATTATTTACAAATCATTATATATTAATCAATTTTTTGCTCGTTTTTTTTTTTTTTTTTACaa                             <  1:970500/122‑1 (MQ=255)
                                                                                             aaaaCAAATATCAATTTTTAAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTATTTTCAAAACATAATCTAATAATCAAATTTATGCCCGGTTTTTTTTTTTTTTTTACaa                             <  2:491966/128‑1 (MQ=255)
                                                                                              aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTaaaaaa                            >  1:3605153/1‑128 (MQ=255)
                                                                                                 ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTaaaaaaaaa                         >  2:4236330/1‑127 (MQ=255)
                                                                                                  tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAata                        >  1:4242175/1‑128 (MQ=255)
                                                                                                  taatataaattttttAAAAAATTTTTAATTTTAATAAATTTATTTTTTTTTAAATTTTTAAATTTTTTTAAAATAATTATATATTAATAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAAAata                        <  1:6256283/119‑1 (MQ=255)
                                                                                                     tttttattttttTAAAAATTTTTAATTTTAATAAATTAATTTTTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTATTTTCGTTTTTTTTTTTTTTTTACAAAAATATAat                    <  1:2766864/122‑1 (MQ=255)
                                                                                                     taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTAAAAAAAATATaa                     >  2:2710136/1‑128 (MQ=255)
                                                                                                     taGCACTATTTATAAAAATTTTTAATATTAAAAACAGAAATTATTTTCAAATTTTTAAATTATTTTAAAATAATTATCTATTAATCAAATTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATaa                     <  1:7303628/128‑1 (MQ=255)
                                                                                                     attcaattttttTAAAAATTTTTATTTTTAATAAATTTATTTATTTTAATATTTTTTAATTATTTTAAAATCATTATATATTAATAAATTTTATGCTTTTTTTTTTTTTTTTTTTACAAAAATATAat                    <  2:3013736/122‑1 (MQ=255)
                                                                                                      aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATTTAat                    >  1:3377779/1‑128 (MQ=255)
                                                                                                       gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAACAAAAATATAat                    >  2:5158074/1‑128 (MQ=255)
                                                                                                       gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAAAATAatt                   >  2:311931/1‑128 (MQ=255)
                                                                                                        cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCTTTTTTTTTTTTTTTTTTAaaaaaaaaataaat                  >  2:1880570/1‑120 (MQ=255)
                                                                                                        cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAaatt                  >  2:9647110/1‑128 (MQ=255)
                                                                                                        cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAAAAAAatta                  >  2:80186/1‑127 (MQ=255)
                                                                                                         aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATATAattta                 >  2:3315008/1‑128 (MQ=255)
                                                                                                         aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATAAAattta                 >  1:13826/1‑128 (MQ=255)
                                                                                                         aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTAACAAAAAAATAatttt                 >  1:8408033/1‑127 (MQ=255)
                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATAAAatttttt               >  1:2454419/1‑128 (MQ=255)
                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATATAatttatt               >  2:3906324/1‑128 (MQ=255)
                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑‑‑TTTTTTTTTTTTTACAAAAATATAATTTATTTAAt           >  2:5628183/1‑128 (MQ=255)
                                                                                                            tttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTACAAAAATATAatttatt               >  2:530260/1‑128 (MQ=255)
                                                                                                            tttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATATAaattattt              >  2:4414835/1‑128 (MQ=255)
                                                                                                                   aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTAATTTAATaaaa       >  2:9068216/1‑128 (MQ=255)
                                                                                                                    aaaTTTTTAATTTTAATAAATTTATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTTTATATTAATAAATTTTATTCTTGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAAc      <  1:5963727/128‑1 (MQ=255)
                                                                                                                    aaaTTTTTAATTTTAATAAATGAATTATTTTTAAAATTTTTAAATTATTTTCAAATAATTATCTATTAATTAATTTTATGCT‑‑TGTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGt    <  1:646472/128‑1 (MQ=255)
                                                                                                                     aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                   >  1:501553/1‑52 (MQ=255)
                                                                                                                       tttATAATTTTAATAAATGAATTATTTTTAAAATTTTTAAATTATTTTAAAATCATTATATATTAATAAAATTTATGCTCGTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACAtaa  <  1:8025441/128‑2 (MQ=255)
                                                                                                                          ||                                                                                                                              
TAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTAT  >  CP016816/420035‑420282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: