BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	165,218	A→T	intergenic (+182/‑360)	JCVSYN2_00665 → / → JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	165,218	0	A	T	96.6%	4655.3 / 14.4	1629	intergenic (+182/‑360)	JCVSYN2_00665/JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base A (39/16); new base T (646/928); total (685/944)
Fisher's exact test for biased strand distribution p-value = 1.86e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1682 total aligned reads displayed.

TATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAA  >  CP016816/165091‑165344

tATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATAGCAATTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTATATA‑TTTTTTGTATATGAAAATATTa                                                                                                                                >  2:1019932/1‑128 (MQ=255)

   ttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTtaaa                                                                                                                             <  1:3010789/128‑1 (MQ=255)

    tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTTTATATGCAAATTTTtaaaa                                                                                                                            >  1:2165153/1‑127 (MQ=255)

    tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTtaaaa                                                                                                                            >  2:1366737/1‑127 (MQ=255)

          aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                      >  1:1389882/1‑128 (MQ=255)

           aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                     >  2:1198603/1‑128 (MQ=255)

            ttGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAaattaatt                                                                                                                    <  1:1947319/128‑1 (MQ=255)

               tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                 >  1:1806386/1‑128 (MQ=255)

                aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                >  2:2630189/1‑128 (MQ=255)

                   actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                             >  2:1486790/1‑128 (MQ=255)

                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGt                                                                                                           >  2:1446715/1‑128 (MQ=255)

                     tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                           >  2:1906048/1‑128 (MQ=255)

                      ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                          <  2:2221857/128‑1 (MQ=255)

                      ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                          >  2:1745444/1‑128 (MQ=255)

                      ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                          >  1:605329/1‑128 (MQ=255)

                         cttttttttaataTTTATTTTGATTAATTTAAATCAATTGTTTTAAATATTAATAAACTAAAAATATTATATTTTTACATA‑TTTTTTGTATATGCAAATTTTTTAAAAATTAATTTTAAATTTTaaaa                                                                                                       <  1:2113349/120‑1 (MQ=255)

                         ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGTATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                       <  2:1963995/128‑1 (MQ=255)

                          tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                      >  2:1144603/1‑128 (MQ=255)

                          tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                      >  2:229479/1‑128 (MQ=255)

                           aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTaaaaaa                                                                                                     >  1:871412/1‑128 (MQ=255)

                           aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTATAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                     >  2:376879/1‑128 (MQ=255)

                            gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAt                                                                                                    >  2:844753/1‑128 (MQ=255)

                             tttataataTTTATTTTCATTAATTTATAGCATTTTTATGATATAGTAATAAACATAAACTATTTTTTTTTTAGTTA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATa                                                                                                   <  2:706435/125‑1 (MQ=255)

                             ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATa                                                                                                   >  2:2979931/1‑128 (MQ=255)

                               gATAATATTTAGTTTCATTAATTTATCGCACTTTTATGAATTAGTAAGAAACTAAAACTATTTTATTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAAAAGc                                                                                                 <  1:3042871/128‑1 (MQ=255)

                               gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                 >  1:2794614/1‑128 (MQ=255)

                                ataataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCt                                                                                                <  1:2554135‑M2/128‑2 (MQ=255)

                                 taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                   >  2:1537492/1‑108 (MQ=255)

                                  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAttt                                                                                              <  2:770619‑M2/128‑4 (MQ=255)

                                  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                              <  1:1178410‑M2/128‑4 (MQ=255)

                                   atatTTAGTTTAATTAATTTATCGCACTTGTATGAATTAGTAAGAAAATAAAACTATTTTCTTTTTAGTTA‑TTTTTTGTATATGCTAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                             >  2:939797‑M2/1‑124 (MQ=255)

                                      ttttGTTTAATTAATTTTTCTCAATTTTTAGAATTATTAATAAAATAAAAATATTATATTTTTAGATA‑TTTTTTTTTTATGCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGCttttttt                                                                                          <  1:739359/128‑1 (MQ=255)

                                      tttAGTTTCAATAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATATTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAttttttt                                                                                          <  1:2041739‑M2/128‑8 (MQ=255)

                                      tatagTTTCATTAATTTATCGCACTTGTAATAATTAGTAATAAAATAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCttttttt                                                                                          >  2:577359‑M2/3‑121 (MQ=255)

                                        tattttCATTAATTTATAGCACTTGTATGAATTAGTAAGAAAAAAAAACTATTATCTTTTTAGATA‑TTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                        <  1:1305931/125‑1 (MQ=255)

                                        tattttCAATAATTTATAGCACTTGTATTAATTAGTAATAAAATAAAAATATTATCTTTTTATATA‑ATTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                        <  2:2382626/125‑1 (MQ=255)

                                        tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                        <  1:2965043‑M2/128‑10 (MQ=255)

                                        tAGGTTCATTAATTTATCGCACTTGTATTTATTACTAATAAAATAATAATTATATATTTTTTTATA‑TTTTTTTTAAATTCAAATTTTTAAAAAATTAATTTGCACTTGTAAAAAATACCttttttttt                                                                                        <  1:1001110/128‑1 (MQ=255)

                                         attttAATTAATTTATCGCAATTGTATGAATTATTAATAAACTAAAAATATTATATTTTTATATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGCtttttttttt                                                                                       <  1:52995/126‑1 (MQ=255)

                                          gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttttt                                                                                      <  1:2502492‑M2/128‑12 (MQ=255)

                                           tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttt                                                                                     <  2:1614361‑M2/128‑13 (MQ=255)

                                             tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                   <  2:1085374‑M2/128‑15 (MQ=255)

                                              cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGTAAAAAATAGCtttttttattttttt                                                                                  <  1:1123852‑M2/128‑16 (MQ=255)

                                               aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                 >  2:892749/1‑128 (MQ=255)

                                               aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttta                                                                                 <  2:445390‑M2/128‑17 (MQ=255)

                                                 tAATTTATAGCACTTTTATTAATTAGTAATAAACTAAAACTATTATCTTTTTATATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATACCtttttttttttttttata                                                                               <  2:2666331/128‑4 (MQ=255)

                                                    tttATAGCCATTGTATGTAATAGTTATAAACTAAAAATATTATCTTTTTATATA‑TTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCttttttttttttttttataat                                                                            <  1:1713582/128‑6 (MQ=255)

                                                     ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttaaattta                                                                           <  2:3026170‑M2/128‑23 (MQ=255)

                                                     ttATCGCAATTGTATGAATTAGTAATAAAATAAAACTATTTTTTTTTTATATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAATAGCtttttttttttttttataatgg                                                                           >  1:2595397‑M2/1‑106 (MQ=255)

                                                       aTCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttatattttaa                                                                         <  1:1220161‑M2/128‑25 (MQ=255)

                                                            atttttATTAATAATTAAAAAAATAAAAAAA‑TATATTTTTATATATTTTTTTTTATATTAAATTTTTTAAAAAATTAATTTTAAATT‑TAAAAAAAA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATaa                                                               <  2:1261546/123‑1 (MQ=255)

                                                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatgtaattataa                                                                   <  1:185716‑M2/128‑31 (MQ=255)

                                                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatgtaaatataa                                                                   <  2:2433069‑M2/128‑31 (MQ=255)

                                                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttatattggaaatataaa                                                                  <  1:2393898‑M2/128‑32 (MQ=255)

                                                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataattgaaatataaa                                                                  <  2:2494372‑M2/128‑32 (MQ=255)

                                                               tttattaattattaatTAACTAAAAATTTTATTTTTTTTGATA‑TTTTTTTTTTATTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAA‑‑‑‑‑TTTTGTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                            <  1:2257829/126‑1 (MQ=255)

                                                               tGTATGAATTATTAAGAAACTAAAAATATTATCTTTTTATATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAAATTTAACTTTTAAAAAATAGCatttttttttttataatggaaatataaatttt                                                                 >  2:3061533‑M2/1‑96 (MQ=255)

                                                               tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttataatttaaaaataaaat                                                                 <  2:1320973‑M2/128‑33 (MQ=255)

                                                                gttttaaTTATTAATCAACTATAACTATTATATTTTTATTAA‑TTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAt                                                             <  1:1607333/123‑1 (MQ=255)

                                                                gatagAATTAGTAAGAAAAAAAAAATATTTTTTTTTTAGTTA‑TTTTTTTTATATCCAAAATTTAAAAAAATTAATTTTAACTTGTAAAAAATACC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                            <  2:1649262/124‑1 (MQ=255)

                                                                gTATGAATTAATAAGAAAATAAAACTATTATATTTTTATATA‑TTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAAAAGCtttttttttttttttataatggaaatataaatt                                                                >  1:2463145‑M2/1‑95 (MQ=255)

                                                                  aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttatatttgaaatataaatttta                                                              <  2:3170490‑M2/128‑36 (MQ=255)

                                                                        tattaataaAATAAAATTATTTTTTTTTTATATA‑TTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                    <  1:1044749/125‑1 (MQ=255)

                                                                        tAGTAATAAAATAAAAAAATTATATTTTTAGATA‑TTTTTTTTATATGCAAATTTTTTAAAAATTTATTTGAACTTGTAAAAAATAGCtttttttttttttataatggaaatataaattttaggtattt                                                        >  1:385910‑M2/1‑87 (MQ=255)

                                                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttatattttaaaaataaattttaggta                                                        <  1:961034‑M2/128‑42 (MQ=255)

                                                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatgtaaatataaattttatttat                                                        <  2:2915204‑M2/128‑42 (MQ=255)

                                                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttaaaaatgaaaaaataaatttaagtatt                                                        <  2:2071563‑M2/128‑42 (MQ=255)

                                                                         aGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttatatatgaaatttaaaattttagttatttt                                                       <  1:926359‑M2/128‑43 (MQ=255)

                                                                          gTAAGAAATTTAAACTTTTATTTTTTTATATA‑TTTTTTGTATATTCAAATTTTTAAAAAATTTATTTGAAATTTTAAAAAATATCtttttttttttttttataatggaaatataaattttaggtattt                                                      >  1:2328315‑M2/1‑85 (MQ=255)

                                                                          gTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttaataatgctaatataaattttatgtattt                                                      <  1:3123845‑M2/128‑44 (MQ=255)

                                                                               aaaataaaactaTTTTTTTTTTATATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtattttgttg                                                 >  1:2662984‑M2/1‑80 (MQ=255)

                                                                                aactaaaactaTTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtatttttttgt                                                <  1:1985058‑M2/128‑50 (MQ=255)

                                                                                 aataatacctttattttttttgttA‑TTTTGTGTATTTTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtattttgttgtc                                               >  2:2002741‑M2/11‑78 (MQ=255)

                                                                                 aataaaaataTTTTTTTTTTTTATT‑TTTTTTTTTTATGAAAATTTTTAAAAAATTTATTTTTAATTTTTAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                            <  1:1440268/126‑1 (MQ=255)

                                                                                  ctaaaactaTTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttttttttttataatgtaaatataaattttatttattttttttta                                              <  2:2826815‑M2/128‑52 (MQ=255)

                                                                                  ctaaaactaTTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttatattttaattataaattttagttatttttttgaat                                              <  1:79559‑M2/128‑52 (MQ=255)

                                                                                    aaatctaTTATCTTTTTATTTA‑TTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaa                                            >  2:329105‑M2/1‑75 (MQ=255)

                                                                                    aaaaataTTATTTTTTTTTTAA‑TTTTTTTTTAATGAAAATTTTTAAAAAATAAATTTTAATTTTTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                       <  2:2881633/128‑1 (MQ=255)

                                                                                       aattttatttttttttatt‑tttttTGTTTATTCAAATTTTTTAAAAATTTATTTTAATTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                     <  1:1553664/124‑1 (MQ=255)

                                                                                        ctaTTATATTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAAATAATTTGAACTTGTAAAAAAATGCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtat                                        >  1:2730599‑M2/1‑71 (MQ=255)

                                                                                         tattatCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttaatgtaaaaataaattttagttatttttttttctaagtat                                       <  1:679243‑M2/128‑59 (MQ=255)

                                                                                         tattatCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttataatgtaaatataaattttagttattttgttttctaattaaat                                       <  1:1755075‑M2/128‑59 (MQ=255)

                                                                                         tattatATTTTTATATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatct                                       >  2:1695850‑M2/1‑70 (MQ=255)

                                                                                         atatatcttTTTTTATT‑TTTTTTGTATAAGCAAATTTTTTAAAAATTTATTTGAACTTTTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatc                                       >  2:650259‑M2/4‑70 (MQ=255)

                                                                                           ttatCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatttaaatataaatttttattatttttttgtaaaattatata                                     <  2:255646‑M2/128‑61 (MQ=255)

                                                                                            tatTTTTTTTTATA‑TTTTTTTTTTAATTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaa                                <  1:2860667/128‑1 (MQ=255)

                                                                                                   ttAGATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttaatggaaatataaattttatttatttttttttataattatataaaaaaatat                             <  2:3099585‑M2/128‑69 (MQ=255)

                                                                                                      gATA‑TTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatgtaaatataaattttatttattttgttttataattatataaaaaaatatat                          <  2:808863‑M2/128‑72 (MQ=255)

                                                                                                         a‑tTTTTTGTATATTTAAATTTTTAAAAAATTAATTATAAATTGTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaagg                       >  2:187118‑M2/1‑54 (MQ=255)

                                                                                                           ttttttttATTTGCAAATTTTTTAAAAATTAATTTTAAATTGTAAAAAATAGCttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaa                      >  1:2198723‑M2/1‑53 (MQ=255)

                                                                                                               ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttaaatgtaaatataaattttagttattttgttttataattataaaaaaaaatagaaagtaaaaa                  <  1:468724‑M2/128‑80 (MQ=255)

                                                                                                                tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttatattgtaaatataaattttagttatttttttttataattatctaaaaaaatataaaggaaaaaa                 <  2:528133‑M2/128‑81 (MQ=255)

                                                                                                                  taaaatcaaatttTTAAAAAATTAAATTTTAATTGTAAAATATATCtttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaatatt               >  2:1809773‑M2/7‑46 (MQ=255)

                                                                                                                    tatTCAAATTTTTTAAAAATAAATTTTTAATTTTTAAAAATTTCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaatatt             >  1:3200172‑M2/1‑44 (MQ=255)

                                                                                                                     ttttaaattttTAAAAAATTAATTTTAAATTTTAAAAAATTGTttttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaatatt            >  1:1486790‑M2/5‑43 (MQ=255)

                                                                                                                      tttaaatttTTAAAAAATTAATTTAAAATTTTAAAAAATTTTtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaatattgt           >  2:2591716‑M2/4‑42 (MQ=255)

                                                                                                                         aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttataatttaaatataaattttaggtattttgttgtataagtatataaaaaaatataatgtaaaaaatattgttaaa        <  2:2152488‑M2/128‑90 (MQ=255)

                                                                                                                            ttttTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtattttgttgtataattatctaaaaaaatagaaatgaaaaaatattgttaaaag     <  2:2752003‑M2/128‑93 (MQ=255)

                                                                                                                            ttttAAAA‑ATTTAATTTAAACT‑GTAAAAAAAATCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaatattgttaaaagta   >  1:1659051‑M2/1‑34 (MQ=255)

                                                                                                                            ttttAAAA‑AATTAATTTGAACTTTTTAAAAATTTCttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaatattgttaaaagta    >  1:2905692‑M2/1‑35 (MQ=255)

TATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATA‑TTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAA  >  CP016816/165091‑165344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`