Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA97.8% 5023.9 / 75.3 1624intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (648/933);  minor base . (28/7);  total (682/942)
Fisher's exact test for biased strand distribution p-value = 5.25e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1679 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349
                                                                                                                             |                                                                                                                               
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                               >  1:2624738/1‑128 (MQ=255)
  ttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaact                                                                                                                             <  2:1920889/128‑3 (MQ=255)
    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                           >  1:588630/1‑128 (MQ=255)
    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                           >  1:1389882/1‑128 (MQ=255)
     aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                          >  1:3111116/1‑128 (MQ=255)
       tGTATTACTTTACTAGTTTATAATATTTAGTTTAATTAATTTATCGCAATTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                        <  2:1233637/128‑1 (MQ=255)
            tactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACt                                                                                                                   >  1:2361477/1‑128 (MQ=255)
             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACtt                                                                                                                  >  2:2110536/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                >  1:1305089/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                >  2:1557416/1‑128 (MQ=255)
                ttactATTTGATAATATTTAGTTTCATTAATTTATCTCACTTGTATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                               <  1:172038/128‑1 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                              >  2:289577/1‑128 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                              >  1:377991/1‑128 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                             >  1:976779/1‑128 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAAATATTATCTTTATAGATATTTTATGTATATGCAAATTTTTAAAAAATTAATTTGAACTAGTaaa                                                                                                             >  2:2680256/1‑128 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTATAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                          >  2:376879/1‑128 (MQ=255)
                      gtttttaataTTTAGTTTAATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaaa                                                                                                         <  2:1456746/123‑2 (MQ=255)
                      gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAt                                                                                                         >  2:844753/1‑128 (MQ=255)
                       tttataataTTTATTTTCATTAATTTATAGCATTTTTATGATATAGTAATAAACATAAACTATTTTTTTTTTAGTTATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATa                                                                                                        <  2:706435/125‑1 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTATAAAATAGCttt                                                                                                   <  2:328305‑M2/128‑4 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGTAAAAAATAgatat                                                                                                   >  2:316851/1‑124 (MQ=255)
                             atatTTAGTTTAATTAATTTATCGCACTTGTATGAATTAGTAAGAAAATAAAACTATTTTCTTTTTAGTTATTTTTTGTATATGCTAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                  >  2:939797‑M2/1‑124 (MQ=255)
                                 ttATTTTAATTAATTTTTGGAATTTGTTTGAATTATTAATAAAAAAAAAATATTATATTTTTATATATTTTTTTTATTTGCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGCtttttttt                                                                                              <  1:2692507/128‑1 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                             <  1:2048977‑M2/128‑10 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                             <  1:3040847‑M2/128‑10 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                             <  2:3105055‑M2/128‑10 (MQ=255)
                                  tAGGTTCATTAATTTATCGCACTTGTATTTATTACTAATAAAATAATAATTATATATTTTTTTATATTTTTTTTAAATTCAAATTTTTAAAAAATTAATTTGCACTTGTAAAAAATACCttttttttt                                                                                             <  1:1001110/128‑1 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                           <  2:1391667‑M2/128‑12 (MQ=255)
                                      ttCATTAATTTATCGCAATTGTTTTAATTATTAATAAAATAAAAATATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttt                                                                                         >  1:2993980‑M2/1‑115 (MQ=255)
                                        taTTAATTTATCCCAATTGTATTAATTATTATCAAAATAAAACTATTATTTTTTTATATTTTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATATCttttttttttttttt                                                                                       <  1:778655/127‑1 (MQ=255)
                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAATATAGCttttttttttttttt                                                                                       <  2:1879677‑M2/128‑16 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                      >  2:892749/1‑128 (MQ=255)
                                            aaTTTATTGCACTTTTATGAATTATTAAGAAACTAAAACTTTTATCTTTTTTGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGCttttttttttttttataat                                                                                   <  1:515161/128‑6 (MQ=255)
                                            aaTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttttttataa                                                                                   <  1:933703‑M2/128‑20 (MQ=255)
                                             aTTTATCGCACTTGTATGAATTATTAATAAAATAAAAATATTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAAATAATTTGAACTTGTAAAAAATAGCtttttttttttttttataat                                                                                  >  2:1170875‑M2/1‑108 (MQ=255)
                                             aTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataat                                                                                  <  1:1883469‑M2/128‑21 (MQ=255)
                                             aTTTATCGAACTTGTATGAATTAGTAAGAAAATAAAACTATTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttataatgg                                                                                  >  2:1624855‑M2/1‑108 (MQ=255)
                                              tttATCGCAATTGTATTAATTAGTTATAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCttttttttttttttataatgg                                                                                 >  2:2322874‑M2/1‑107 (MQ=255)
                                               ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatgt                                                                                <  2:104264‑M2/128‑23 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatgaaaat                                                                            <  1:1119728‑M2/128‑27 (MQ=255)
                                                      acgattATGAATTAGTAACAAACTTAAAATATTTTTTTTTTATATATTTTTTTTATATGCAAATTTTTAAAAAAATAATTTTAACTTTTAAAAAAAAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAatat                                                                      <  1:2426426/123‑1 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttaatgtaaaattaa                                                                        <  2:255979‑M2/128‑31 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatttaaatataa                                                                        <  2:1978074‑M2/128‑31 (MQ=255)
                                                        ttGTATGAATTAGTAAGTAACTAAAAATATTATATTTTTATATATTTTTTGTTTATGCAAATATTTAAAAAATTAATTTTAACTTTTAAAAAAAAGCtttttttttttttttataatggaaatataaa                                                                       >  2:2642150‑M2/1‑97 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttaatgtaaatataaa                                                                       <  2:2992838‑M2/128‑32 (MQ=255)
                                                          ttaTGAATTTGTAATAAACTAATAATATTATCTTTTTAGATATTTTTTGTTAATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAATGCtttttttttttttttataatggaaatataaatt                                                                     >  1:2830680‑M2/2‑95 (MQ=255)
                                                          ttATGAATAAGATAGTAAATAAAACTATATACTTTTTTTATAATTTTTTTAAATGCAAAATTTTAAAAAATTTATTTTAACTTGTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  2:1676936/127‑1 (MQ=255)
                                                          gTATGAATTAGGAATAAACATAAACTATTTTCATTTTTTATAATTTTTTTTTATTCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAAAAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                 <  1:228960/128‑1 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttataatggaaatttaaatt                                                                    <  1:2477144‑M2/128‑35 (MQ=255)
                                                             ataaatattaataaACTAAAAAAATTTTATTTTTATATTTTTTTTGTATATGGAAATTTTTAAAAAATTAATTTTTACTTTTTAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                              <  1:894302/120‑1 (MQ=255)
                                                              gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttataatgtaaaaataaattttattt                                                                 <  1:2948446‑M2/128‑38 (MQ=255)
                                                                  ttctAAGATACTAAAAAATTTATGTTTTTAGTTATTTTATGTATATACAAATTTTTAAAAAATTAATTTGAACTTGTAATAAAT‑‑C‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                         <  2:2930967/125‑1 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtat                                                             <  1:1559349‑M2/128‑42 (MQ=255)
                                                                   tttaataaAATAAAAATATTAAATTTTTTTTAATTTTTTTTATTTGCAAATTTTTAAAAAATTAATTTTCACTTTTTAAAAAAA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                         <  1:706436/126‑1 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatttaaatataaattttatttatt                                                            <  2:646427‑M2/128‑43 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatggaattataaaatttaggtatt                                                            <  1:2893390‑M2/128‑43 (MQ=255)
                                                                      aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttttttataattaaaatataaattttagttattttt                                                         <  2:1336264‑M2/128‑46 (MQ=255)
                                                                        gAAAATATAACTATTATCTTTTTTGATTATTTTTGTATATTAAAATTTTTAAAAAATTAATTTTAACTTTTAAATAATA‑‑‑‑TGGTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                   <  2:2019739/128‑1 (MQ=255)
                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataattaaaaaaaaaattttttgtatttttttt                                                      <  1:654470‑M2/128‑49 (MQ=255)
                                                                           actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttataatggaaatataaattttaggtatttttttgtct                                                    <  2:2836982‑M2/128‑51 (MQ=255)
                                                                           aataaaaataTTTTTTTTTTTTATTTTTTTTTTTTATGAAAATTTTTAAAAAATTTATTTTTAATTTTTAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                                 <  1:1440268/126‑1 (MQ=255)
                                                                            ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatggaaatataaattttagttattttgttgtaa                                                   <  2:1723281‑M2/128‑52 (MQ=255)
                                                                             taaaaaatttatttttttttatttttttTTTTTATTTAAATTTTTTAAAAATTAATTTTATCTTTTAAAAAATA‑‑‑‑TATTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                              <  2:3038059/120‑1 (MQ=255)
                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaa                                                 >  2:2395835‑M2/1‑75 (MQ=255)
                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttaataatgtaaatataaaattaaggtatttagttaacta                                                 <  1:1810753‑M2/128‑54 (MQ=255)
                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttataatggaaataaaaattttatttattttgttgtataag                                                 <  1:2000556‑M2/128‑54 (MQ=255)
                                                                               aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttataatggaaatatatatttttgtttttttgttgtgtaagt                                                <  2:1273454‑M2/128‑55 (MQ=255)
                                                                               aaaattttttttttttatatattttttGTATATTGAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATATCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaag                                                >  1:1669626‑M2/12‑74 (MQ=255)
                                                                                  ctaTTATCTTTTTTTTTTTTTTTTTTATATTCAAATTTTTAAAAAATTATTTTTAAATTTTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtat                                             >  1:2566073‑M2/1‑71 (MQ=255)
                                                                                   ttttatCTTTTTTGTTATTTTTTGTTTTTTTAAATTTTTTAAAAATTAATTTGATCTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                         <  2:519387/126‑1 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttaatgtaaataaaaattttatgtatttttttttataattata                                            <  2:3068689‑M2/128‑59 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttataattgaaatataaattttaggtattttgttttataattat                                            <  2:442616‑M2/128‑59 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatataaatataaattttagtttttatgtttaataattataa                                           <  1:453055‑M2/128‑60 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTATTAAAATTAAAATATAAATTTTATATATTTTTTTTTATaataatata                                      >  2:1112100/1‑121 (MQ=255)
                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttataatgtaaatataaattttttgtttttttttttataagtataaaaa                                         <  1:2517362‑M2/128‑62 (MQ=255)
                                                                                         tttttttgATATTTTTTGTATATGTAAATTTTTAAATAATTAATTTGAACTTTTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaa                                      >  1:2139083‑M2/2‑64 (MQ=255)
                                                                                           ttttATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaata                                    >  1:175844‑M2/1‑62 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatgtaaatataaattttagttattttgttttataattatctaaaaaaa                                    <  1:217742‑M2/128‑67 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttataatgtaaatataaattttagttattttgttgtctaattatctaaaaaaat                                    <  2:809116‑M2/128‑67 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTAATTTTTTTTTAAAATTAACAaaaaaa                                 >  1:1951248/1‑128 (MQ=255)
                                                                                            tttAGATATTTTTTGTATATTCAAATTTTTAAAAAAATAATTTTAACTTGTAAAAAATAGGttttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaa                                   >  2:2766863‑M2/1‑61 (MQ=255)
                                                                                              tataaatattttttATATTCAAATTTTTAAAAAAATAAATTTAAATTGTAAAAAATACCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatag                                 >  1:1614361‑M2/6‑59 (MQ=255)
                                                                                                   atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTAATAATTATATAAAAAAATAaaaatta                       >  1:3189947/1‑125 (MQ=255)
                                                                                                    ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttataatggaaatataaattttaggtattttgttttctaagtatataaaaaaatagataggaa                           <  2:771098‑M2/128‑76 (MQ=255)
                                                                                                      ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTATTTATTTTTTTTTATAATTTTATAAAAAAAAATAAAGaaaa                     >  1:2225253/1‑128 (MQ=255)
                                                                                                        ttttATATTCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaa                       >  2:567057‑M2/1‑49 (MQ=255)
                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggttttttgttgtataattatataaaaaaatataaagaaaaaa                       <  2:2181619‑M2/128‑80 (MQ=255)
                                                                                                          tttaatgaaaATTTTAAAAAAATTAATTTTAACTTTTAAAAATTAGCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaat                     >  2:1821201‑M2/5‑47 (MQ=255)
                                                                                                          gTATATTCTAATTTTTTAAAAATTAATTTTAATTTGTTAATAATATCtttttttttttttttataatggaaatatatatttttggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaat                     >  1:1042846‑M2/1‑47 (MQ=255)
                                                                                                           tttatgaAAATTTTTAAAAAATTAATTTTAATTTGTAAAAAATTTCttttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaat                    >  1:1219349‑M2/3‑46 (MQ=255)
                                                                                                            atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatgtaaatataaattttagttatttttttttataagtatataaaaaaatagaaattaaaaaatat                   <  2:2450991‑M2/128‑84 (MQ=255)
                                                                                                            atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaatat                   >  2:1761026‑M2/1‑45 (MQ=255)
                                                                                                              atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTTTTTTTTTGTATAATTATATAAAAAAATATAAAGGAAAAAAtttttt            >  1:276101/1‑126 (MQ=255)
                                                                                                                    aTTTTTTAAAAATTAATTTTAAATTGTTAAAAATATCttttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaatattgttaaa           >  1:1486791‑M2/1‑37 (MQ=255)
                                                                                                                    aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttataatggaaatataaattttaggtattttgttatctaagtatctaaaaaaatagaaagtaaaaaatatttttaat           <  2:2593456‑M2/128‑92 (MQ=255)
                                                                                                                    aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTAGTTATTTTTTTTTATAATAAAATAAAAAAATATAAAGTAAAAAATATTTTTaaa        >  1:1174005/1‑128 (MQ=255)
                                                                                                                     ttttAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAATTATAAATTTTAGTTTTTTTTTTGTATAATTATTTAAAAAAATAAAAATTAAAAAATATTTTTtaaatt     >  2:2253690/1‑126 (MQ=255)
                                                                                                                      tttAAAAAATTTAATTTTAATTTTAAAAAATATGtttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaatattgttaaaagta        >  1:844753‑M2/1‑34 (MQ=255)
                                                                                                                        ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATGTATTTTTTTTTATAATTATATAAAAAAATATAAATTAAAATATATTGTTAAAAGTa    >  2:1499531/3‑128 (MQ=255)
                                                                                                                         taaaaaatTAATTTGAACTTGTAAAAAATAGCttttttttttttttataatggaaatataaattttaggtattttgttgtctaagtatctaaaaaaatagaaaggaaaaaatattgttaaaagtaata      >  2:2499829‑M2/2‑32 (MQ=255)
                                                                                                                             |                                                                                                                               
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: