BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	273,009	A→G	L270S (TTA→TCA)	JCVSYN2_01135 ←	dihydroxyacetone kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	273,009	0	A	G	99.4%	4108.1 / NA	1099	L270S (TTA→TCA)	JCVSYN2_01135	dihydroxyacetone kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (544/547); minor base T (4/3); total (549/550)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1117 total aligned reads displayed.

CTTTATTTTTTACTTGCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTAAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACA  >  CP016816/272884‑273133

cTTTATTTTTTACTTGCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTgaa                                                                                                                            <  2:2021653/128‑1 (MQ=255)

cTTTATTTTTTACTTGCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTgaa                                                                                                                            >  1:1715142/1‑128 (MQ=255)

   tATTTTTTACTTGCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAata                                                                                                                         <  2:620776/128‑1 (MQ=255)

    aTTTTTTACTTGCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAatat                                                                                                                        >  2:2910438/1‑128 (MQ=255)

      tttttACTTGCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATTTCa                                                                                                                      >  1:1001658/1‑128 (MQ=255)

        tttACTTGCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTTACCTTCTGAATGTGAGTGATTATGTACTATTGAAATATCAtt                                                                                                                    >  1:1446860/1‑128 (MQ=255)

         ttACTTGCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTa                                                                                                                   <  2:2627200/128‑1 (MQ=255)

             ttGCTTATTAGCTTGTAAATTCATGTTTTCATTTTTTAGAGTTTGAATATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATcaa                                                                                                               <  2:2242076/128‑1 (MQ=255)

               gCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATcaaca                                                                                                             >  1:1275507/1‑128 (MQ=255)

               gCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGCTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATcaaca                                                                                                             >  1:1345594/1‑128 (MQ=255)

                cTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATcaacaa                                                                                                            >  2:2554867/1‑128 (MQ=255)

                cTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATcaacaa                                                                                                            >  2:2603017/1‑128 (MQ=255)

                cTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTATGAAATATCATTATgaacaa                                                                                                            >  1:929718/1‑128 (MQ=255)

                      ttgttGTAATTTCATGTTTTCAATTTATAGAGTTTGAATATCTACATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAacaactacaa                                                                                                      <  1:454395/125‑1 (MQ=255)

                       gCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAacaactacaac                                                                                                     >  2:242336/1‑128 (MQ=255)

                         ttGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAAcaactacaacta                                                                                                   >  1:2557119/1‑128 (MQ=255)

                         ttGGAAATTCATCTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTATAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAAcaactacaacta                                                                                                   <  1:1666600/128‑1 (MQ=255)

                          tGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACtat                                                                                                  <  1:174406/128‑1 (MQ=255)

                          tGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACtat                                                                                                  <  1:2079769/128‑1 (MQ=255)

                          tGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACtat                                                                                                  >  2:1307315/1‑128 (MQ=255)

                           gTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACtata                                                                                                 >  2:1604160/1‑128 (MQ=255)

                             aaaTTCATGTTCTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGa                                                                                               >  1:3073068/1‑128 (MQ=255)

                               aTTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAg                                                                                             >  1:576617/1‑128 (MQ=255)

                               aTTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGAAAACCTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAg                                                                                             >  1:2027407/1‑128 (MQ=255)

                                 tCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTa                                                                                           <  2:3003629/128‑1 (MQ=255)

                                      ttttCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACCACTATAGAAGTATTTcc                                                                                      >  1:303328/1‑128 (MQ=255)

                                       tttCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTAGAGAAGTATTTcct                                                                                     >  2:3186159/1‑127 (MQ=255)

                                        ttaaaTTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGATTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCaa                                                                                    <  1:1617385/125‑1 (MQ=255)

                                         tCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCaaa                                                                                   <  2:1498498/128‑1 (MQ=255)

                                           aaTTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCaaaaa                                                                                 <  1:1896183/128‑1 (MQ=255)

                                            aTTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAAt                                                                                >  1:1985644/1‑128 (MQ=255)

                                             tttttAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGATAGTGAGTGAGTATGTACTTTTCAAATATCATTATCTACAACTACAACTATAGATGTATTTCCAAAAAtt                                                                               >  1:222565/1‑128 (MQ=255)

                                              ttttAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAAttt                                                                              >  1:381876/1‑128 (MQ=255)

                                               tttAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTg                                                                             <  2:2737929/128‑1 (MQ=255)

                                               tttAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTg                                                                             >  1:1050900/1‑128 (MQ=255)

                                               tttAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTg                                                                             <  1:2290961/128‑1 (MQ=255)

                                                  agagTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCaa                                                                          <  2:751812/128‑1 (MQ=255)

                                                   gagTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAAt                                                                         >  1:889759/1‑128 (MQ=255)

                                                      tttGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGa                                                                      >  2:3040771/1‑128 (MQ=255)

                                                      tttGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGa                                                                      >  2:2331938/1‑128 (MQ=255)

                                                         gAAAATCTCCATATTGTTGTAATAATTGTAAATCTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGAtct                                                                   <  1:3143034/128‑1 (MQ=255)

                                                         gAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTa                                                                                           <  2:324953/104‑1 (MQ=255)

                                                         gAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGAtct                                                                   >  2:2882569/1‑128 (MQ=255)

                                                         gAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGAtct                                                                   >  1:1557216/1‑128 (MQ=255)

                                                         gAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAAGTTGCAATTGAtct                                                                   >  1:2694583/1‑128 (MQ=255)

                                                          aaaaTCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGAtctc                                                                  >  1:29102/1‑128 (MQ=255)

                                                          aaaaTCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGAtctc                                                                  >  2:1459903/1‑128 (MQ=255)

                                                           aaaTCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTCCAATTGATctct                                                                 <  1:1303931/128‑1 (MQ=255)

                                                            aaTCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGATATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTa                                                                >  2:2138081/1‑128 (MQ=255)

                                                             aTCTCCATATTGTTGTAAAATTTGTAACACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTaa                                                               <  1:1114081/128‑1 (MQ=255)

                                                             aTCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTTCTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTaa                                                               >  1:993010/1‑128 (MQ=255)

                                                                tcCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGa                                                                      >  2:395376/1‑118 (MQ=255)

                                                                   atatTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTg                                                         <  1:769159/128‑1 (MQ=255)

                                                                   atatTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTg                                                         >  2:1531893/1‑128 (MQ=255)

                                                                   atatTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTg                                                         <  2:1707386/128‑1 (MQ=255)

                                                                    tatTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTTCAATTGATCTCTAATGACTGa                                                        >  2:2365705/1‑128 (MQ=255)

                                                                    tatTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGa                                                        >  1:2352929/1‑128 (MQ=255)

                                                                       tgttgtAAAAATTGTAAAACTTGACCTTGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGtt                                                     <  2:2505790/128‑1 (MQ=255)

                                                                       tgttgtAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGATATATCATTATCAACAACTACAACTAATGAAGTATTTGCAAAAATTTGCAATTGATCTCTAATGACTGAGtt                                                     >  1:180900/1‑128 (MQ=255)

                                                                         ttgtAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACACCTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGCTCTCGAATGACTGAGtgtt                                                   >  1:1944728/1‑128 (MQ=255)

                                                                         ttgtAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGtttt                                                   >  2:1278267/1‑128 (MQ=255)

                                                                          tgtAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTg                                                  <  1:518239/128‑1 (MQ=255)

                                                                          tgtAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTg                                                  <  2:1847166/128‑1 (MQ=255)

                                                                           gtAAAAATTGTAAAACTTGACCTTGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGt                                                 <  2:1966021/128‑1 (MQ=255)

                                                                           gtAAAAATTGTAAAACTTGACCTGGTGATAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGt                                                 <  1:1387336/128‑1 (MQ=255)

                                                                            tAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTa                                                <  1:3178523/128‑1 (MQ=255)

                                                                             aaaaaTTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTaa                                               <  2:908602/128‑1 (MQ=255)

                                                                                    gTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATttatta                                        >  2:3112336/1‑128 (MQ=255)

                                                                                      aaaaCTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAAt                                      <  2:671314/128‑1 (MQ=255)

                                                                                       aaaCTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAAtt                                     <  1:2472512/128‑1 (MQ=255)

                                                                                         aCTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCa                                   >  1:1477884/1‑128 (MQ=255)

                                                                                          cTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAAt                                                                                >  2:1930347/1‑82 (MQ=255)

                                                                                           ttGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTTATTCAat                                 >  2:560894/1‑128 (MQ=255)

                                                                                           atGACATGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAat                                 <  1:665477/127‑1 (MQ=255)

                                                                                            tGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAatc                                >  2:1160251/1‑128 (MQ=255)

                                                                                            tGACCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAatc                                <  2:1092511/128‑1 (MQ=255)

                                                                                            tGACCTGGAGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAatc                                <  2:2788721/128‑1 (MQ=255)

                                                                                            agACCTGTAGAATGTGTGTGAGTATGTACTTTTGATATTTCATTATACTCAACTAGAAGTATAGATGTATTTCCAAAAATTTGCATTTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAatc                                <  1:1233315/127‑1 (MQ=255)

                                                                                              aCCTGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAatcat                              >  2:2187324/1‑128 (MQ=255)

                                                                                                 tGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATcatcat                           >  2:1221221/1‑128 (MQ=255)

                                                                                                 tGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATcatcat                           >  1:1199584/1‑128 (MQ=255)

                                                                                                 tGGTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATcatcat                           <  1:2814882/128‑1 (MQ=255)

                                                                                                  ggTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCAtt                          <  2:848930/128‑1 (MQ=255)

                                                                                                   gTGAAAGTGAGTGAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCAttt                         >  2:1046439/1‑128 (MQ=255)

                                                                                                    tGAAAGTGAGTGAGTATGTACTTTAGATATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGAACTCTAATGACTGACATTTGTAATTTATTAATTCAATCATCATTTa                        >  2:474293/1‑128 (MQ=255)

                                                                                                       cagtgagtgAGTATGTACTTTTGAAATCTCATTATCAACCACTACAACTATCGAAGTATTTCCAAAAATTTGCAATTGATCTCTCATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCa                     <  1:2175503/127‑1 (MQ=255)

                                                                                                        agtgagtgAGTATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCAt                    >  1:70736/1‑128 (MQ=255)

                                                                                                          tgagtgagTATGTACTTTTGATATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTAGTAATTTATTAATTCAATCATCATTTAGCATaa                  >  2:2275144/1‑128 (MQ=255)

                                                                                                              tgagtATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAg                       >  2:136452/1‑119 (MQ=255)

                                                                                                                agtATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAg            >  1:2654145/1‑128 (MQ=255)

                                                                                                                  tATGTACTTTTGAAATATCATTATCAACAACTACTACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCt          <  2:808054/128‑1 (MQ=255)

                                                                                                                  tATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCt          <  1:2603017/128‑1 (MQ=255)

                                                                                                                  tATGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTGATTATATCAATCTTCATTTAGCATAACAAGAGCt          >  1:2993376/1‑128 (MQ=255)

                                                                                                                   aTGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCtt         >  1:2739922/1‑128 (MQ=255)

                                                                                                                   aTGTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCtt         >  2:2688618/1‑128 (MQ=255)

                                                                                                                     gTACTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCa       <  2:1763113/128‑1 (MQ=255)

                                                                                                                      tACTTTTGAAATATGATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAg      <  1:837809/128‑1 (MQ=255)

                                                                                                                       aCTTTTGAAATATCATTATCAACACCTACAACGATTGAAGTATTTCCAAAAATTTGCAATTGATCGCTAAGGACGGTGGTTTGTAATTTATTAATTCAATCATCCTTTAGCAGAACAATTGCTTCAGt     >  1:2863963/1‑128 (MQ=255)

                                                                                                                        cTTTTGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTa    <  1:242336/128‑1 (MQ=255)

                                                                                                                          tttGAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTACCATAACAATAGCTTCAGTACa  >  2:2078873/1‑128 (MQ=255)

CTTTATTTTTTACTTGCTTATTAGCTTGTAAATTCATGTTTTCAATTTTTAGAGTTTGAAAATCTCCATATTGTTGTAAAAATTGTAAAACTTGACCTGGTGAAAGTGAGTGAGTATGTACTTTTAAAATATCATTATCAACAACTACAACTATAGAAGTATTTCCAAAAATTTGCAATTGATCTCTAATGACTGAGTTTTGTAATTTATTAATTCAATCATCATTTAGCATAACAATAGCTTCAGTACA  >  CP016816/272884‑273133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`