Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,157 2 bp→TT intergenic (‑440/+402) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1570AT99.4% 4384.1 / NA 1402intergenic (‑440/+403)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (3/5);  new base T (731/663);  total (734/668)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1580AT98.1% 4218.6 / NA 1402intergenic (‑441/+402)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (4/23);  new base T (730/645);  total (734/668)
Fisher's exact test for biased strand distribution p-value = 5.94e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1471 total aligned reads displayed.

TAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420035‑420285
                                                                                                                           ||                                                                                                                                  
 aaattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattt                                                                                                                               >  2:1367803/1‑127 (MQ=255)
   attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTATAAAACTACTATCACTTTTTTAAAAAATttttaatttta                                                                                                                             >  2:1546792/1‑125 (MQ=255)
    ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                            <  2:1491060/128‑5 (MQ=255)
     tcattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaat                                                                                                                           <  1:2138893/128‑6 (MQ=255)
     tcattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaat                                                                                                                           <  1:2067459/128‑6 (MQ=255)
     tcattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaat                                                                                                                           >  2:261302/1‑123 (MQ=255)
     tcattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaat                                                                                                                           <  1:305780/128‑6 (MQ=255)
      cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                          <  2:516120/128‑1 (MQ=255)
      cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                          <  1:2495679/128‑1 (MQ=255)
      cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                          <  1:274166/128‑1 (MQ=255)
      aattcatACTTTTATTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                          <  1:795081/127‑1 (MQ=255)
        ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                        <  2:2934666/128‑1 (MQ=255)
         tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                       <  1:637447/128‑1 (MQ=255)
            tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                    <  1:2367793/128‑1 (MQ=255)
             aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                   <  2:2401988/128‑1 (MQ=255)
             aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTAATAGCTCTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                   >  1:1697880/1‑128 (MQ=255)
             aCTATTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                   <  1:402269/128‑1 (MQ=255)
              cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                  <  1:154310/128‑1 (MQ=255)
              cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                  <  1:2849613/128‑1 (MQ=255)
               ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                 <  1:3125271/128‑1 (MQ=255)
                 ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                               >  2:604065/1‑128 (MQ=255)
                    gTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAttttt                                                                                                            <  2:420281/128‑1 (MQ=255)
                     tctcATAAATCTAATTTTCTAAAAATATTTTATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                           <  2:881155/128‑1 (MQ=255)
                      ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                          >  1:2624782/1‑128 (MQ=255)
                        cATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                        <  2:1603707/128‑1 (MQ=255)
                         aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                       >  1:2726394/1‑128 (MQ=255)
                         aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                       >  2:1303744/1‑128 (MQ=255)
                          tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                      >  1:2797601/1‑128 (MQ=255)
                           aaaTCTAAGTATCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                     <  2:2235291/128‑1 (MQ=255)
                            aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                    <  2:2726394/128‑1 (MQ=255)
                                tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                <  2:268227/128‑1 (MQ=255)
                                 aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTGAat                                                                                               <  2:2770200/128‑1 (MQ=255)
                                 aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                               <  1:1269155/128‑1 (MQ=255)
                                     ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                           >  2:2975100/1‑128 (MQ=255)
                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                         <  1:1060594/128‑1 (MQ=255)
                                       cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                         >  1:1000950/1‑128 (MQ=255)
                                        tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTTAAAAATTTTTAATTTTAATAACTGAATGATTTTTAAAATTGTTAAATTATTTTc                                                                                        >  1:686015/1‑128 (MQ=255)
                                        tAAAATTATTTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                        <  1:966104/128‑1 (MQ=255)
                                         aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                       <  1:1438409/128‑1 (MQ=255)
                                          aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                      <  1:1359293/128‑1 (MQ=255)
                                          aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                      <  2:2573077/128‑1 (MQ=255)
                                           aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAATTGTTAAATTATTTTCaaa                                                                                     >  2:715945/1‑128 (MQ=255)
                                             ttattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATc                                                                                   >  2:2182007/1‑128 (MQ=255)
                                              tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCAATTTTTTAAAAAATTATTAATTAAAATAACTTAATTATTAAACAAATTGTAAAATTATTTTCAAATCa                                                                                  >  2:2132073/1‑128 (MQ=255)
                                                 tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                               >  1:3015125/1‑128 (MQ=255)
                                                  ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                              <  2:1984798/128‑1 (MQ=255)
                                                  ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                              >  1:1580861/1‑128 (MQ=255)
                                                  ttATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                              <  1:1739292/128‑1 (MQ=255)
                                                    aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                            <  1:2447613/128‑1 (MQ=255)
                                                    aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                            >  1:266862/1‑128 (MQ=255)
                                                     tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                           <  2:815771/128‑1 (MQ=255)
                                                      cATTTAAAAAAATAATACTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                         >  1:348293/1‑128 (MQ=255)
                                                       aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                         >  1:3095941/1‑128 (MQ=255)
                                                         ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                       <  2:2509977/128‑1 (MQ=255)
                                                          tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAt                                                                      >  1:3170072/1‑128 (MQ=255)
                                                               aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                                 <  1:505059/128‑1 (MQ=255)
                                                                aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                               <  2:1666776/97‑1 (MQ=255)
                                                                 ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                               <  1:1960663/128‑1 (MQ=255)
                                                                     taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATga                                                           >  2:1129406/1‑127 (MQ=255)
                                                                      aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTAttat                                                          >  2:91695/1‑125 (MQ=255)
                                                                       tttttttttGTTGATATTTTATAAAACTAATAGCACTTTTTTTAAAAAATTTTAATTTTAATAACTGAATTTTTTTTCAAATTGTTAAATTATTTTCAAATCATTTTATATTAATCAATTTTATGCTc                                                        <  1:1650340/127‑1 (MQ=255)
                                                                       cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTc                                                        >  2:2848295/1‑128 (MQ=255)
                                                                       cTTTTATTAGTTGATATATTAGAAAACTACTAGCACATTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTc                                                        >  2:3100719/1‑128 (MQ=255)
                                                                         tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGt                                                    >  2:3051647/1‑128 (MQ=255)
                                                                          ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                       <  2:373031/63‑1 (MQ=255)
                                                                           tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttt                                                  >  1:2964723/1‑128 (MQ=255)
                                                                           tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtta                                                  >  1:2564176/1‑127 (MQ=255)
                                                                           tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTAAAATAATTATCTATTAATCAATTTTATGCTCGttt                                                  >  2:1429855/1‑128 (MQ=255)
                                                                             tttttttatttattatAAAAATACTATCAATTTTTTAAAAAATTTTTTATTTTAATTAATTTATTATTTTTCAAATTGTTAAATTATTTAAAAATCATTTTCTATTAAACAATTTTAAGCTCGttttt                                                <  2:2307362/118‑1 (MQ=255)
                                                                              taGTTTATATATTATAAAACTAATATCAATTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttt                                               <  2:108905/128‑1 (MQ=255)
                                                                                gTTGATATATTATAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAAAAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATAAATCAATTTTATGCTCGtttttttt                                             <  2:2442052/128‑1 (MQ=255)
                                                                                 ttttaaaatTATAAAAATAATATCAATTTTTTAAAAAATTTTTAATTTTAAAAAATTAAATATTTTTAAAATTGTTAAATTATTTTCAAAACATTAAAAATTAATCAAATTTATGCCCGttttttttt                                            <  2:3192628/121‑1 (MQ=255)
                                                                                  ttatatatTAGAAAACTAATATCAATTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTTTTAAATTATTTTCAAATCAATAACTATTAAACAATTTTATGCTCGtttttttttt                                           <  2:1254356/126‑1 (MQ=255)
                                                                                    atatttTATAAAACAAATAGAAATTTTTTAAAAAATTTTTAATTTTAAAAAATTAATTATTTTTAAAATTTTTAAAATATTTTAAAATCATTATATTTTAATAAATTTTATGCTCGtttttttttttt                                         <  1:562266/128‑1 (MQ=255)
                                                                                     tataaTAGAAAACTACTATAAATTTTTTAAAAAAATTTTATTTTTAATAAATGAATTATTTTTAAAATTTTTAAATTATTTTCAAATAATTATATATTAATAAATTTTATGCTCGttttttttttttt                                        <  2:1938885/128‑1 (MQ=255)
                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttttttttttt                                       >  1:1132715/1‑128 (MQ=255)
                                                                                      atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttttttttttt                                       >  2:1767620/1‑128 (MQ=255)
                                                                                       tatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttttttttttttttt                                      >  1:2878772/1‑128 (MQ=255)
                                                                                           aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTATTTTTTTTTTTTTTTTTTaa                                  >  2:2050194/1‑127 (MQ=255)
                                                                                              aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttttttttttttttttttaaaa                               >  2:3129370/1‑123 (MQ=255)
                                                                                               aactaaattcacaTTTATAAAAAATTATTAAATTTAATAACTTAATTATTTTTTAAATTGTTAAATTATTTTTAAATAAATATCTATTAAAAAATTTTATGCTCGTTTTTTTTTTTTTTTTACaaaaa                             <  2:1216918/119‑1 (MQ=255)
                                                                                                actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTaaaaaaaa                             >  1:1201157/1‑128 (MQ=255)
                                                                                                actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTATTTTTTTAaaaaaa                             >  2:2088737/1‑128 (MQ=255)
                                                                                                 ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTACaaaaa                             >  2:301180/1‑128 (MQ=255)
                                                                                                  tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTAAAAAAatat                          >  2:2652792/1‑128 (MQ=255)
                                                                                                  taatttaaattttttAAAAAATTTTTTATTTTAATTAATTTATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTTTATTTTAATAAATTTTATGTTCGTTTTTTTTTTTTTTTTTACAAAAata                           <  1:2212280/119‑1 (MQ=255)
                                                                                                   actaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAatat                          >  2:2893469/1‑128 (MQ=255)
                                                                                                     tattaattttttAAAAAATTTTTAATTTTAATAACTTTATTTTTTTTTAAATTTTTAAATTTTTTTTAAATAATTATATATTAATAAATTTTTTTCTCGTTTTTTTTTTTTTTTTTACAAAAATATaa                        <  2:1713676/122‑1 (MQ=255)
                                                                                                      atatattttttAAAAAATTTTTAATTTTAATATTTTAATTTTTTTTTAAATTTTTAATTTTTTTTAAAATAATTTTTTATTTATTAATTTTATTTTATTTTTTTTTTTTTTTTTTTAAAAAAATATaa                        <  2:1894338/123‑1 (MQ=255)
                                                                                                         aattttttAAAAATTTTTAATTTTAAAAAATTAATTATTTTTAAAATTTTTAAATTATTTTTAAATAATTATATATTAATAAATTTTATTCTCG‑‑TTTTTTTTTTTTTTTACAAAAATATAatttattt                 <  2:663572/126‑1 (MQ=255)
                                                                                                         aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCTTTTTTTTTTTTTTTTTTACAAAAATATTattta                    >  2:1020086/1‑128 (MQ=255)
                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                         <  1:1885122/60‑1 (MQ=255)
                                                                                                           ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATAAAatttatt                  >  2:569201/1‑128 (MQ=255)
                                                                                                             ttttAAAAAATTTTTATTTTTAATTAATTAATTTTTTTTTAAATTTTTAAATTATTTTTATATAATTATTTATTAATAAATTTTATTCTTGTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa               <  1:844110/128‑1 (MQ=255)
                                                                                                             ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATATAatttatttt                >  2:47510/1‑127 (MQ=255)
                                                                                                             ttttAAAAAATTTTTAATTTTAATAAATTATTTTTTTTTAAAATTTTTAAATTATTTTTAAATTTTTTTTTATTAATAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTg                <  1:1823465/128‑1 (MQ=255)
                                                                                                               tttaaaaaTTTTTAATTTTTATAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTTTTTATTAATAAATTTTTTTGTCTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa               <  1:73543/125‑1 (MQ=255)
                                                                                                                taataaATTTTTAATTTTAATAAATTAATTTATTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTTATTTTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt             <  2:1853540/128‑1 (MQ=255)
                                                                                                                    aaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATCATCTTTTTTTTTTTTTTTTTTTAAAAAAAAATATTTATTTTATTaaaa         >  2:180933/1‑127 (MQ=255)
                                                                                                                     aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAAAATAATTAATTTTATaaaaat        >  2:760468/1‑126 (MQ=255)
                                                                                                                           ||                                                                                                                                  
TAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420035‑420285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: