Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 420,163 2 bp→TT intergenic (‑446/+396) JCVSYN2_01895 ← / ← JCVSYN2_01900 30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,1630AT99.4% 4378.9 / NA 1402intergenic (‑446/+397)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (4/5);  new base T (730/660);  total (734/668)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP016816420,1640AT98.6% 3861.6 / NA 1393intergenic (‑447/+396)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (2/18);  new base T (729/644);  total (731/662)
Fisher's exact test for biased strand distribution p-value = 1.23e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1411 total aligned reads displayed.

CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATG  >  CP016816/420041‑420289
                                                                                                                           ||                                                                                                                                
cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                              <  1:3097869/128‑1 (MQ=255)
  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                            <  2:2934666/128‑1 (MQ=255)
  ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                            >  2:876663/1‑128 (MQ=255)
   tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTAAAAAAATTTTTAATTTTAATAACt                                                                                                                           >  1:642728/1‑128 (MQ=255)
      tACTTTTATTATAATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                        <  1:2909774/128‑1 (MQ=255)
       aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAATCAACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                       >  2:229749/1‑128 (MQ=255)
       aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                       <  2:2401988/128‑1 (MQ=255)
       aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTAATAGCTCTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                       >  1:1697880/1‑128 (MQ=255)
       aCTATTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                       <  1:402269/128‑1 (MQ=255)
         ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                     <  2:1766873/128‑1 (MQ=255)
          tttAGTCTCATAAATCTATGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                    <  1:795512/128‑1 (MQ=255)
          tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                    <  2:2513412/128‑1 (MQ=255)
                 tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                             <  1:1288361/128‑1 (MQ=255)
                  cATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                            <  2:1603707/128‑1 (MQ=255)
                  cATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                            >  1:2147705/1‑128 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                           >  2:1303744/1‑128 (MQ=255)
                   aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                           <  2:2628766/128‑1 (MQ=255)
                    tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAAtt                                                                                                          >  1:506348/1‑128 (MQ=255)
                      aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                        <  1:687922/128‑1 (MQ=255)
                      aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                        <  2:1893985/128‑1 (MQ=255)
                      aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                        <  1:56447/128‑1 (MQ=255)
                       aTCTTAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                       <  2:1546278/128‑1 (MQ=255)
                          tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                    <  2:604206/128‑1 (MQ=255)
                            aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                  <  1:2682438/128‑1 (MQ=255)
                             gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                                 >  2:1366782/1‑128 (MQ=255)
                              tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                                >  1:2647813/1‑128 (MQ=255)
                              tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                                <  1:1550795/128‑1 (MQ=255)
                               ttatAAAATTATTTTATCATTTAAAAATATAATACTTTTATTATTTTATATATTAGAAAAATACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                               <  1:3180754/125‑1 (MQ=255)
                               ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                               >  2:2728740/1‑128 (MQ=255)
                               ttCTAAAATTATTTTATCAATTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                               <  1:2732367/128‑1 (MQ=255)
                                tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                              <  1:349604/128‑1 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                             >  1:1000950/1‑128 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                             <  1:1911348/128‑1 (MQ=255)
                                 cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                             >  1:2820287/1‑128 (MQ=255)
                                  tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                            <  1:3069101/128‑1 (MQ=255)
                                  tAAAATTATTTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                            <  1:966104/128‑1 (MQ=255)
                                   aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                           >  1:1993813/1‑128 (MQ=255)
                                   aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                           <  1:1438409/128‑1 (MQ=255)
                                    aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                          <  2:431823/128‑1 (MQ=255)
                                     aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAATTGTTAAATTATTTTCaaa                                                                                         >  2:715945/1‑128 (MQ=255)
                                            ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                                  <  2:1984798/128‑1 (MQ=255)
                                            ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                                  >  1:2092689/1‑128 (MQ=255)
                                            ttATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                                  <  1:1739292/128‑1 (MQ=255)
                                             tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                 <  2:3054144/128‑1 (MQ=255)
                                             tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                                 <  1:2242733/128‑1 (MQ=255)
                                              aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                                >  1:2507307/1‑128 (MQ=255)
                                              aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                                >  2:2978575/1‑128 (MQ=255)
                                               tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                               <  2:815771/128‑1 (MQ=255)
                                                cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                              <  2:2448414/128‑1 (MQ=255)
                                                   ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                           >  2:3100773/1‑128 (MQ=255)
                                                    tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAt                                                                          >  1:73593/1‑128 (MQ=255)
                                                          aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                   <  2:1666776/97‑1 (MQ=255)
                                                          aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                                    >  1:2978151/1‑128 (MQ=255)
                                                              ataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATg                                                                >  2:208664/1‑128 (MQ=255)
                                                               taCTTTTATTAGTTGATATATTTGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGc                                                               >  2:1935477/1‑128 (MQ=255)
                                                               taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATga                                                               >  2:1129406/1‑127 (MQ=255)
                                                                aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCt                                                              >  2:526640/1‑128 (MQ=255)
                                                                 tttttttttGTTGATATTTTATAAAACTAATAGCACTTTTTTTAAAAAATTTTAATTTTAATAACTGAATTTTTTTTCAAATTGTTAAATTATTTTCAAATCATTTTATATTAATCAATTTTATGCTc                                                            <  1:1650340/127‑1 (MQ=255)
                                                                 cTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTc                                                            >  2:2848295/1‑128 (MQ=255)
                                                                   tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                     >  1:2853771/1‑70 (MQ=255)
                                                                   tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGt                                                         >  2:3198142/1‑128 (MQ=255)
                                                                    ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtt                                                        >  1:1590607/1‑128 (MQ=255)
                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCtttt                                                       >  1:856096/1‑128 (MQ=255)
                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCtttt                                                       >  2:572807/1‑128 (MQ=255)
                                                                     tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTAAAATAATTATCTATTAATCAATTTTATGCTCGttt                                                       >  2:1429855/1‑128 (MQ=255)
                                                                        taGTTTATATATTATAAAACTAATATCAATTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttt                                                    <  2:108905/128‑1 (MQ=255)
                                                                         aGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGttttttt                                                   >  2:148487/1‑128 (MQ=255)
                                                                            ttatatatTAGAAAACTAATATCAATTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTTTTAAATTATTTTCAAATCAATAACTATTAAACAATTTTATGCTCGtttttttttt                                                <  2:1254356/126‑1 (MQ=255)
                                                                                atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttttttttttt                                            >  2:2131229/1‑128 (MQ=255)
                                                                                atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGtttttttttttttt                                            >  1:2764321/1‑128 (MQ=255)
                                                                                     aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTATTTTTTTTTTTTTTTTTTaa                                       >  2:2050194/1‑127 (MQ=255)
                                                                                       aaaaCTACTAGAAATTTTTTAAAAAATATTAAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATAATTTTAAAAACATAAACTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTACaaa                                   <  2:2164898/128‑1 (MQ=255)
                                                                                         aactaaattcacaTTTATAAAAAATTATTAAATTTAATAACTTAATTATTTTTTAAATTGTTAAATTATTTTTAAATAAATATCTATTAAAAAATTTTATGCTCGTTTTTTTTTTTTTTTTACaaaaa                                 <  2:1216918/119‑1 (MQ=255)
                                                                                         aaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTaaaaaa                                  >  1:1136219/1‑128 (MQ=255)
                                                                                          actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTaaaaaaaa                                 >  1:1201157/1‑128 (MQ=255)
                                                                                            tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAata                               >  2:2662549/1‑128 (MQ=255)
                                                                                            tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTAAAAAAatat                              >  2:2229070/1‑128 (MQ=255)
                                                                                             actaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAatat                              >  2:2893469/1‑128 (MQ=255)
                                                                                               tattaattttttAAAAAATTTTTAATTTTAATAACTTTATTTTTTTTTAAATTTTTAAATTTTTTTTAAATAATTATATATTAATAAATTTTTTTCTCGTTTTTTTTTTTTTTTTTACAAAAATATaa                            <  2:1713676/122‑1 (MQ=255)
                                                                                               taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTATAAAAAAATATaa                            >  2:3133284/1‑128 (MQ=255)
                                                                                                atcACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTGTTAAATTATTTTAAAATTATTAAATATTAATAAATTTTATGCTGGTTTTTTTTTTTTTTTTTTACAAAAATATaa                            <  2:916589/126‑1 (MQ=255)
                                                                                                aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAAaatatt                           >  1:2599504/1‑126 (MQ=255)
                                                                                                 gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTAAAAAAATATAat                           >  2:2701554/1‑128 (MQ=255)
                                                                                                  cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATATAattt                         >  2:476917/1‑128 (MQ=255)
                                                                                                  cACTTTTTTAAAAAATTTATAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCTATTTTATGCTCGATTTTTTTTTTTTTTTTATCAAAAATAAAatt                          >  2:2794114/1‑128 (MQ=255)
                                                                                                   aattttttAAAAAATTTTTAATTTTTATTAATTAATTATTTTTTAAATTTTTAAATTTTTTTTAAATTATTATATATTAATTAATTTTATTCTCGTTTTTTTTTTTTTTTTTACAAAAATATAattta                        <  1:1363731/126‑1 (MQ=255)
                                                                                                   aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCTTTTTTTTTTTTTTTTTTACAAAAATATTattta                        >  2:1020086/1‑128 (MQ=255)
                                                                                                   aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAaattaaatta                        >  2:2580363/1‑128 (MQ=255)
                                                                                                     ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATATAttttatt                      >  2:379850/1‑128 (MQ=255)
                                                                                                       ttttAAAAAATTTTTAATTTTAATAAATTATTTTTTTTTAAAATTTTTAAATTATTTTTAAATTTTTTTTTATTAATAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTg                    <  1:1823465/128‑1 (MQ=255)
                                                                                                       ttttAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa                   <  2:3017521/128‑1 (MQ=255)
                                                                                                         ttAAAAAATTTTTAATTTTTATAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTATCTATTAATAAATTTTTTTGTATTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt                 <  1:1075819/128‑1 (MQ=255)
                                                                                                          taataaATTTTTAATTTTAATAAATTAATTTATTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAATTTTTATTTTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt                 <  2:1853540/128‑1 (MQ=255)
                                                                                                          tAAAAATTTTTTAATTTTAATAAATTTAATTTTTTTTAAATTGTTAAAATTTTTTAAAATAATTATTAATTAATAAATTTTTTTTTCGTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTa                <  1:2469607/128‑1 (MQ=255)
                                                                                                          tAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTAAAAAAATAAAATTTTTTTGAt                  >  2:664143/1‑128 (MQ=255)
                                                                                                           aaaaaaTTTTTTATTTTAATAACTTAATTATTTTTCAAATTTTTAAATTTTTTTAAAATAATTATCTATTAATCAACTTTATGCTCG‑‑‑TTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAAc             <  1:3035895/128‑1 (MQ=255)
                                                                                                           aaaaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTAATTTTAtaa                >  1:2407272/1‑126 (MQ=255)
                                                                                                              aaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATCATCTTTTTTTTTTTTTTTTTTTAAAAAAAAATATTTATTTTATTaaaa             >  2:180933/1‑127 (MQ=255)
                                                                                                              aaaTTTTTAATTTAAATATATTTATTTTTTTTAAATATTTTAAATTTTTTTAAAATAATTATTTTTTAATAAATTTTATTCTGTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTaaa              <  1:3129370/128‑1 (MQ=255)
                                                                                                               aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAAAATAATTAATTTTATaaaaat            >  2:760468/1‑126 (MQ=255)
                                                                                                                           ||                                                                                                                                
CATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATG  >  CP016816/420041‑420289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: