Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,801 A→G intergenic (‑110/+61) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,8010AG97.9% 13861.2 / NA 3823intergenic (‑110/+61)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (19/0);  major base G (1786/1921);  minor base T (63/17);  total (1885/1938)
Fisher's exact test for biased strand distribution p-value = 4.39e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4152 total aligned reads displayed.

AAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484675‑484925
                                                                                                                               |                                                                                                                            
 aaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCa                                                                                                                            >  2:1003101/1‑128 (MQ=255)
     gTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCTATTATATAATTATATTTTTTATAAAAATAAAAATCAAATa                                                                                                                        >  2:471069/1‑128 (MQ=255)
     gTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTa                                                                                                                        <  2:243754/128‑1 (MQ=255)
     gTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTa                                                                                                                        >  2:2086982/1‑128 (MQ=255)
       cAAAACTCATTGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGATCAAAGCAGAAGCTCTTAAGAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                                      <  1:2465287/128‑1 (MQ=255)
       cAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                                      <  2:5199751/128‑1 (MQ=255)
          aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTATTTTAATATAAAAAACAATTAAacaa                                                                                                                   >  2:390378/1‑127 (MQ=255)
             tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAcacaca                                                                                                                <  1:6135083/128‑1 (MQ=255)
             tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACaa                                                                                                               >  1:2722576/1‑128 (MQ=255)
             tCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATATTATAATTATATTTTTTTTTAATATAAAAACAAAGTAACcacaca                                                                                                                >  1:5765881/1‑128 (MQ=255)
               aTAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaa                                                                                                              <  2:6567977/128‑1 (MQ=255)
                    tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAca                                                                                                          >  1:1482931/1‑127 (MQ=255)
                    tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                         <  2:842474/128‑1 (MQ=255)
                    tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                         >  1:3725625/1‑128 (MQ=255)
                     ttAATCATTTTCTAGTTGACCAAAAAATAAGATAGATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                                        <  2:1941247/128‑1 (MQ=255)
                      tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                                       >  2:6414719/1‑128 (MQ=255)
                        aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccc                                                                                                     <  1:5863472/128‑1 (MQ=255)
                        aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTAATTAATTATATTTTATTTTAATATAAAAAGCAAATAAACACACAAAAACTCAcac                                                                                                     >  2:3334212/1‑126 (MQ=255)
                          ccTTTTATAGGAGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                                   <  2:4183401/128‑4 (MQ=255)
                            ttttATAGTTGACCAAAAAATAAGATAGAGCATAGCATAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                                 <  1:4044274/128‑1 (MQ=255)
                             tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATTTAAAAAGCAATTAAACACACAAAAACTCCCCCATTAt                                                                                                >  1:2343247/1‑128 (MQ=255)
                               tataGTTGAACAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCTATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                              <  1:7861543/128‑1 (MQ=255)
                                  aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                           >  2:1619903/1‑128 (MQ=255)
                                   gTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAg                                                                                          <  2:4974052/128‑1 (MQ=255)
                                   gTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTAAACAACTAAACTACACTCGATTATATAATTATATTTTAATTTAATATAAAAAGAAAATAAACACACAAAAACTCACCAATAATgttcat                                                                                          >  2:2542226/1‑123 (MQ=255)
                                      gACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGttt                                                                                       >  1:17396/1‑128 (MQ=255)
                                        ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                                     <  2:6267342/128‑1 (MQ=255)
                                         cAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGGTTaaa                                                                                    >  1:4188569/1‑128 (MQ=255)
                                           aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  2:1313595/128‑1 (MQ=255)
                                           aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATATAATTATATTTTTTTTTAATATAAAAAACAATTAAAAACACAAAAACTAACACAATTTAGTTAGTATaaaaa                                                                                  >  1:5990061/1‑128 (MQ=255)
                                            aaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAat                                                                                 <  2:6080263/128‑1 (MQ=255)
                                            aaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAat                                                                                 <  2:7674833/128‑1 (MQ=255)
                                             aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                                <  2:5666812/128‑1 (MQ=255)
                                                  aGATATAGCAAAGCAGAAGCTCTTAACAACTAAACAACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                           <  1:5606522/128‑1 (MQ=255)
                                                  aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTGGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAAACACATAAACTCACCCATTATAGTGAGTTTAAAAattattaa                                                                           >  2:5796299/1‑126 (MQ=255)
                                                  aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                           <  2:6966361/128‑1 (MQ=255)
                                                  aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                           >  2:3602263/1‑128 (MQ=255)
                                                  aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAGGGTGAGTTTAACTATTATTCa                                                                           >  1:3583285/1‑128 (MQ=255)
                                                   gATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaa                                                                          <  2:1777686/128‑1 (MQ=255)
                                                   gATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACAATCGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaa                                                                          <  2:3057903/128‑1 (MQ=255)
                                                    aTAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaa                                                                         >  2:4565713/1‑128 (MQ=255)
                                                     tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaa                                                                        <  1:1003101/128‑1 (MQ=255)
                                                     tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaa                                                                        >  1:3275085/1‑128 (MQ=255)
                                                     tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACAAACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                                       >  1:530874/1‑128 (MQ=255)
                                                      agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                                       <  2:2311960/128‑1 (MQ=255)
                                                       gagCATAGCAGAAGCTTTTAACAACTAAACTACTCTCGATTATTTACTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                                      <  2:7823902/128‑1 (MQ=255)
                                                       gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                                      >  1:6324808/1‑128 (MQ=255)
                                                       gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                                      >  2:73697/1‑128 (MQ=255)
                                                       gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAATAACCCATTATGGTGAGATTAAAAATTATTCAAAAAt                                                                      >  1:4788541/1‑128 (MQ=255)
                                                       gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTAAATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAATATGTTGAGTTTAAAAATTATTCAAAAAt                                                                      >  1:6597324/1‑128 (MQ=255)
                                                        agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAACTTATTCAAAAAtt                                                                     >  1:7760203/1‑128 (MQ=255)
                                                        agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAACtt                                                                     >  2:5359501/1‑128 (MQ=255)
                                                        agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                                     <  1:545724/128‑1 (MQ=255)
                                                           aTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                                  >  1:1873795/1‑128 (MQ=255)
                                                           aTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                                  <  1:7193137/128‑1 (MQ=255)
                                                           aTAGCAGAAGCTCTTAACAAATAAACTACACTCGAATATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                                  <  1:2039229/128‑1 (MQ=255)
                                                            tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                                 >  1:1154486/1‑128 (MQ=255)
                                                             aGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTTTCGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                                >  1:5019078/1‑128 (MQ=255)
                                                             aGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                                >  2:5507383/1‑128 (MQ=255)
                                                              tgctatgCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAGTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                               >  1:7594212/7‑128 (MQ=255)
                                                                aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                             <  1:2189794/128‑1 (MQ=255)
                                                                aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                             <  1:877311/128‑1 (MQ=255)
                                                                     ctctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                                        >  2:4068025/1‑128 (MQ=255)
                                                                     ctctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                                        <  2:6687902/128‑1 (MQ=255)
                                                                     ctctTAACAACTAAACTACAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                                        <  2:7217393/128‑1 (MQ=255)
                                                                        ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                                        >  1:1606180/1‑77 (MQ=255)
                                                                        ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                                     >  1:6901110/1‑128 (MQ=255)
                                                                           acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                                  >  1:13183/1‑128 (MQ=255)
                                                                           acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                                  <  2:4312507/128‑1 (MQ=255)
                                                                           acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                                  <  2:220606/128‑1 (MQ=255)
                                                                            caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTg                                                 >  2:6838830/1‑128 (MQ=255)
                                                                            caacTAAAATACACTCGATTATTTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTg                                                 <  2:7512420/128‑1 (MQ=255)
                                                                             aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                                <  1:3028712/128‑1 (MQ=255)
                                                                                taaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGc                                             <  1:7471491/128‑1 (MQ=255)
                                                                                  aacttCTCTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                                           <  1:6737004/128‑1 (MQ=255)
                                                                                  aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAGTTGAAGCtg                                           <  2:5076841/128‑2 (MQ=255)
                                                                                  aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                                           <  2:5918429/128‑1 (MQ=255)
                                                                                     taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                                        <  1:3129255/128‑1 (MQ=255)
                                                                                       cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                                      <  2:347747/128‑1 (MQ=255)
                                                                                            gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAATAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                                 <  1:2846717/128‑1 (MQ=255)
                                                                                            gATTATTTAATTATATTTATTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTGGAGCTTCaat                                 <  1:2586011/128‑2 (MQ=255)
                                                                                              ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTa                               >  2:2899806/1‑128 (MQ=255)
                                                                                               tatataAATATATATTTTTAAAATATAAAAAGCAAATAAACACACAAAAACTCACAAATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTaa                              <  1:5161135/128‑1 (MQ=255)
                                                                                                  ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                           <  1:4891639/128‑1 (MQ=255)
                                                                                                  ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                           <  1:4475851/128‑1 (MQ=255)
                                                                                                   tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa                          <  2:2718508/128‑1 (MQ=255)
                                                                                                   tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa                          >  1:7349493/1‑128 (MQ=255)
                                                                                                        atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt                     >  2:4441563/1‑128 (MQ=255)
                                                                                                        atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt                     >  2:4833334/1‑128 (MQ=255)
                                                                                                        atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt                     >  1:4353341/1‑128 (MQ=255)
                                                                                                            ttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt                 >  2:3201144/1‑128 (MQ=255)
                                                                                                             tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt                <  1:7052002/128‑1 (MQ=255)
                                                                                                             tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt                <  1:6478075/128‑1 (MQ=255)
                                                                                                               tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTGATTTaaaataatcaaat              <  1:343096/128‑13 (MQ=38)
                                                                                                                 ttttaatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttctt             >  2:1145751/10‑128 (MQ=255)
                                                                                                                    taatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAg          <  1:4657875/122‑1 (MQ=255)
                                                                                                                     aatataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGc         <  1:3894913/123‑1 (MQ=255)
                                                                                                                      atataaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGct        <  2:1472936/124‑1 (MQ=255)
                                                                                                                           aaaagCAATTAAACACACAAAAACTCACCCATTATGGAGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCttcttc   <  2:3752188/128‑1 (MQ=255)
                                                                                                                            aaagCAATTAAACACACTAACACTCACCCATTATGGTGAGTTTAAATATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTtcttct  <  2:7090857/128‑1 (MQ=255)
                                                                                                                               |                                                                                                                            
AAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484675‑484925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: