Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.6% 7444.6 / ‑1.7 2566intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (10/52);  new base T (1610/894);  total (1620/946)
Fisher's exact test for biased strand distribution p-value = 1.73e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2659 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346
                                                                                                                              |                                                                                                                                 
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                 <  2:831861/128‑1 (MQ=255)
   aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                              <  2:4254322/128‑3 (MQ=255)
    cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                             >  1:5974475/1‑128 (MQ=255)
    cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                             <  2:6553011/128‑1 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTTGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            <  1:4744848/128‑1 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                            >  2:558898/1‑128 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                          <  2:6432016/128‑1 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                          >  1:2569776/1‑128 (MQ=255)
        aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                         <  2:1165519/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  2:7733487/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                      <  1:5858248/128‑1 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGaa                                                                                                                   >  2:2185347/1‑128 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                   <  1:5315708/128‑1 (MQ=255)
               ttactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAc                                                                                                                  <  2:5962813/128‑1 (MQ=255)
                tactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACt                                                                                                                 >  2:3219006/1‑128 (MQ=255)
                 actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                                >  1:886417/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTTTATGAATTAGTAAGAAACAAAAACTATTATCTTATTAGATATTTATTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGt                                                                                                              >  1:181515/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGAAAATTTTTAAAAAATTAATTTTAACtttt                                                                                                              >  2:3385331/1‑126 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGt                                                                                                              >  1:7186793/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              <  1:7734974/128‑1 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                              >  1:2318934/1‑128 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                            >  2:3062633/1‑128 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                            <  2:6251423/128‑1 (MQ=255)
                     tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTCTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                            <  1:3338129/128‑1 (MQ=255)
                      actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           <  2:7249777/128‑1 (MQ=255)
                      actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTATTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           >  2:2471197/1‑128 (MQ=255)
                      actAGTTGATAATATTTAGTATCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                           <  2:3852758/128‑1 (MQ=255)
                       ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                          >  1:5463501/1‑128 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                         >  2:1921744/1‑128 (MQ=255)
                           ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                      >  2:1064260/1‑128 (MQ=255)
                            tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGACACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                     >  2:2314557/1‑128 (MQ=255)
                            tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                     >  2:1330934/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGGATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATATTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                >  2:1563078/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                >  2:6115750/1‑128 (MQ=255)
                                   atttatttTAATTAATTTATCTCACTTGTATGAATTATTAAGAAACTAAAAATATTATCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATATCtttttt                                                                                              <  1:2712416/128‑1 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttta                                                                                           >  2:3987681/1‑127 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                         >  1:2899719/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                         >  2:751800/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAAATAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttatttttt                                                                                         >  2:7643042/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAAATAATTTGAACTTGTAAAAAAAAGCtttttttttttttttt                                                                                    <  2:5887679/128‑1 (MQ=255)
                                                 aTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATATCttttttttttttttttttat                                                                                >  2:5209642/1‑126 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttttttttttttata                                                                               >  1:2453096/1‑125 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttata                                                                               >  1:1363954/1‑125 (MQ=255)
                                                     aTCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTATTTTTTtataattt                                                                            >  2:5457856/1‑124 (MQ=255)
                                                      tctcACTTTTATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGTTATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGa                                                                         <  2:4691649/125‑1 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATgaa                                                                         >  1:4586280/1‑126 (MQ=255)
                                                       agCACTTTTATTAATTTTTAATAAAAAAAAACAATTATATTTTTATATATTTTTTTTATATTAAAATTTTTAAAAAATTAATTTTAAAATTTAAAAAAAATCTTTTTTTTTTTTTTTTTTTATAATgg                                                                          <  2:6876586/127‑1 (MQ=255)
                                                           cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCTTTTATTTTTTTTTTTTTTATAAtttaaaa                                                                      >  2:1688471/1‑122 (MQ=255)
                                                            ttttttttattattAATAAAATAAAACAATTATATTTTTATTTATTTTTTTTTTATTCAAATTTTTAAAAAAATAAATTTAAAATTTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                   <  1:624223/120‑1 (MQ=255)
                                                                tttaaTTAGTAATAAACTAAAAATATTTTTTTTTTATATATTTTTTGTATATGAAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                               <  1:6365326/125‑1 (MQ=255)
                                                                aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAATAAAAAt                                                                >  1:6129193/1‑128 (MQ=255)
                                                                 tttattattAATAAAATAAAAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTCAAATTTTTTAAAAATTAATTTTTACTTGTAAAAAATTGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                               <  1:6536232/125‑1 (MQ=255)
                                                                  taaTTAGTAATAAAATAAAAATATTATATTTTTTTATTTTTTTTTTTTATTAAAATTTTTTAAAAATTAATTTTAAATTGTAAAAAAAAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAttt                                                              <  2:2872207/127‑1 (MQ=255)
                                                                     ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAAAAATTTAAATATAAATTTTat                                                           >  2:5737112/1‑127 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTATTTTTTTTTTTTATAATGAATATATAATTTTTATGTa                                                        >  1:3042919/1‑128 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTTAAATTTAAAAATAAAATTTaatt                                                         >  1:4070490/1‑125 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAAAAGTAAATATAAATTTTatg                                                          >  1:1003399/1‑126 (MQ=255)
                                                                          aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAAAATAAAATTAATGTAtt                                                     >  1:5647180/1‑128 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTTAATTTAAATTTTATGTAttttt                                                  >  1:200992/1‑127 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTGAAATTTAAATTTTATTTAttttt                                                  >  2:7845988/1‑127 (MQ=255)
                                                                              aaatatatatattttatttTTTTATTTTTTTTTTATTTTTAAATTTTTTAAAAATTTATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTttgtt                                                <  2:181515/119‑1 (MQ=255)
                                                                               aataaaaataTTAACTTTTTATATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGTACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgttg                                               <  2:3521743/126‑1 (MQ=255)
                                                                                ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTATTAAAAAATTAATTTGATCTTGTAAAATATAGCTTTTTTTTATTTTTTTTTTAT‑‑TGTAATAATAAATTTTAGTTATTatgtttt                                              >  2:5092085/1‑126 (MQ=255)
                                                                                 taaaaattTTTTTTTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                              <  2:206348/128‑1 (MQ=255)
                                                                                 taaaaataTTTTTTTTTTATTTTTTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                              <  1:7878670/128‑1 (MQ=255)
                                                                                   aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATttttttttat                                            >  2:494450/1‑124 (MQ=255)
                                                                                    aaattttatATTTTTATTTTTTTTTTTTATATGTAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                         <  1:3816197/123‑1 (MQ=255)
                                                                                     atatttatTTTTTTTTATATTTTTTTTTATTTAAATTTTTAAAAAAATAAATTTTAATTTTTAAAAATTATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                           <  2:700697/124‑1 (MQ=255)
                                                                                     acttTTATATTTTTATATATTTTTTGTAAATGCAAAATTTTAAAAAATTAATTTTTACTTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                          <  1:4274371/128‑1 (MQ=255)
                                                                                     aaatatataTTTTTATATATTTTTTTTATATTTAAATTTTTAAAAAATTTATTTTTAATTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                          <  1:4405510/123‑1 (MQ=255)
                                                                                      tttttttttttttATTTTTTTTTTGTATATTAATATTTTTATAAAATTAATTTTAAATTTTTAAAAATATATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                         <  1:6947619/120‑1 (MQ=255)
                                                                                      atattttTTTTTTATATATTTTTTTTTTTTTTAAATTTTTAAAAAATTTATTTTAAATTTTTAAAAA‑‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                      <  1:1183515/127‑1 (MQ=255)
                                                                                        tttttattttttTATATTTTTTTTTTATTTAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                       <  2:3625082/122‑1 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTa                                       >  2:2735745/1‑128 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTAGTTTTTtttttgaaatataaaa                                     >  1:3184031/1‑118 (MQ=255)
                                                                                           atCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAAATTTTTTTATTTTTTTGTATAATTaaata                                   >  1:3965928/1‑124 (MQ=255)
                                                                                            tCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAT‑‑TTTAAATATATATTTTATGTAATATTTTTTATAATTATCTaaa                                 >  2:2590166/1‑128 (MQ=255)
                                                                                                tttATATTTTTTTTTTTTTTTGAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaa                               <  1:7329495/128‑1 (MQ=255)
                                                                                                tttATATATTTTTTGTATTTTCAAATTTTTAAAAAATTAATTTTAAATTTTTAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                             <  2:5346937/128‑1 (MQ=255)
                                                                                                tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAATAATGTAAATATAAATTTTATTTTTTTTTTTGTATAATTATATaaaaa                               >  2:4398585/1‑128 (MQ=255)
                                                                                                   aGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTTTTTTTTTTTATAATTTTATAAAAAAAt                            >  2:4814011/1‑128 (MQ=255)
                                                                                                    taaatTTTTTTTATATTTAAATTTATATAAATTTAATTTTTAAATTTAAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                          <  2:4130088/125‑1 (MQ=255)
                                                                                                     attttttttttATATTAAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAAATTATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                          <  2:4538065/125‑1 (MQ=255)
                                                                                                      ttttttttttATTTTAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                          <  1:2159381/126‑1 (MQ=255)
                                                                                                      tatTTTTTTTATTTTTAATTTTTTAAAAAATTTATTTTTATTTTTTAAAAATTGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                         <  2:146509/128‑1 (MQ=255)
                                                                                                         tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTGTTTTATAAGTATAAAAAAAAATATaaatt                     >  2:7152979/1‑126 (MQ=255)
                                                                                                          ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTTTTAATTTTTTTTTATAATTATATAAAAAAATAaaaat                      >  1:3686402/1‑127 (MQ=255)
                                                                                                            ttttataTGCAAATTTTTTAAAAATTATTTTTTACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                  <  1:1725489/125‑1 (MQ=255)
                                                                                                               tttttttaaaTTTTTAAAAAATTAATTTTAACTTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                 <  2:7417802/121‑1 (MQ=255)
                                                                                                                   tttaaatttttTAAAAATTTATTTTTAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat              <  1:1500455/121‑1 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTATTTTTTTTTTTTTAAAATGTAAATATAAATTTTAGTTATTTTTTTTTATAATTAACTAAAAAAATAGAAAGTaaaaaaaa             >  1:4904608/1‑126 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGTAAATAAAATTTTTTTTATTTTTTTTGTTAAATTAATTAAAAAAATAAAATATAAAAAatat            >  1:708525/1‑128 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTTTTTATTTTTTTGTATAATTATATAAAAAAATTAAAAGAAAAAAatat            >  1:7618768/1‑128 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATATAAATATAAATTTTAGTTATTTTGTTGTAAAATTAATAAAAAAATTAGAAATTAAAatatat            >  1:5121980/1‑128 (MQ=255)
                                                                                                                    gCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTTTCAAAGTAACTAAAAAAATAGAAATGAAAAAATAttt          >  1:3495013/1‑127 (MQ=255)
                                                                                                                     ctaaTTTTTAAAAAAATAATTTGTAATTTTAAAAAATAGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTg          <  2:6690756/126‑1 (MQ=255)
                                                                                                                     cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAAATTTAAATTAAAATTTATTTTTTTTTTTTTTTTAATTTTAAAAAAAAAAAAAAAAAAAAAAATAtt           >  1:6811498/1‑128 (MQ=255)
                                                                                                                     cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTGTTTAATTATATAAAAAAATATAAATAAAAAAAAAttt          >  2:3732988/1‑127 (MQ=255)
                                                                                                                        aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAAAAAAAATTTTATTTTTTTTTTTGTAATAGTATAAAAAAAAAAAAAAATAaaaaaaaaatttta       >  1:6639045/1‑120 (MQ=255)
                                                                                                                        aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAATATTTTTaa      >  1:7470896/1‑128 (MQ=255)
                                                                                                                              |                                                                                                                                 
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: