Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 242,857 G→A G829D (GGT→GAT)  JCVSYN2_01035 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816242,8570GA99.7% 10961.1 / NA 3409G829D (GGT→GAT) JCVSYN2_01035ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (1651/1742);  minor base T (2/7);  total (1659/1750)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3409 total aligned reads displayed.

AAGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGGTTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGATTAAT  >  CP016816/242732‑242981
                                                                                                                             |                                                                                                                            
 aGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGAttt                                                                                                                           >  1:7707518/1‑128 (MQ=255)
    aCTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGc                                                                                                                        >  2:2388599/1‑128 (MQ=255)
    aCTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGc                                                                                                                        >  2:6265951/1‑128 (MQ=255)
    aCTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAAAATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTAGTTCAGCAGATTTAGc                                                                                                                        >  2:3380152/1‑128 (MQ=255)
      ttGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCaa                                                                                                                      <  1:2869370/128‑1 (MQ=255)
       tGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAt                                                                                                                     <  2:1069406/128‑1 (MQ=255)
        gATTTAAAAAAGCTAAGTTTACTACTAAATTTGGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAtt                                                                                                                    <  1:7194269/128‑1 (MQ=255)
        gATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAtt                                                                                                                    <  2:599487/128‑1 (MQ=255)
          tttAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTTGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTcc                                                                                                                  <  1:2709316/128‑1 (MQ=255)
             aaaaaaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGTTAAAAAAAATAACTTTATATTAGTAACTGTTTTTGTAATTTCAACTATGTTTATTTCAGCAGATTTATAAATTACATc                                                                                                               >  2:3094154/1‑128 (MQ=255)
             aaaaaaGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATc                                                                                                               <  2:692893/128‑1 (MQ=255)
               aaaaTCTATGTTTACTAATATATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAATTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCaa                                                                                                             <  1:3556308/128‑1 (MQ=255)
                 aaGATAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAAtt                                                                                                           <  2:879957/128‑1 (MQ=255)
                  aGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTg                                                                                                          <  2:423798/128‑1 (MQ=255)
                       agattACTACTTAATTTAGTTTAACTTTAGATTCTTATTGTAAAAAAAATATCTTTTTATTAGTAAATGTTGTTTGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtttt                                                                                                     <  2:7233693/125‑1 (MQ=255)
                       aGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtttt                                                                                                     >  2:2791264/1‑128 (MQ=255)
                       aGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtttt                                                                                                     <  1:2098394/128‑1 (MQ=255)
                        gattACTACTAAATTTAGTTTAACTTAATCTTCTTCTGGAAAAAAAAATAACTTTATATAAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCAATCAATTGCTTTTa                                                                                                    >  1:7316381/3‑128 (MQ=255)
                        gTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTa                                                                                                    >  2:5395418/1‑128 (MQ=255)
                        gTTTACTAATAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTATCTATTGTTGGAATTTCAACTATTATTATTTCAGCATATTTAGAAATTCAATAAATTGCtttaa                                                                                                    >  2:4113836/1‑126 (MQ=255)
                           tactacTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTa                                                                                                 <  1:6315672/128‑1 (MQ=255)
                             ctactaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTAtt                                                                                               >  1:1606355/1‑128 (MQ=255)
                               actaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGAAATTCCATCAATTGCTTTTACTATTaa                                                                                             >  2:7889481/1‑128 (MQ=255)
                                ctaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaa                                                                                            <  1:759405/128‑1 (MQ=255)
                                 taAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaaa                                                                                           <  2:7635252/128‑1 (MQ=255)
                                 taAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaaa                                                                                           <  1:5691224/128‑1 (MQ=255)
                                  aaaTTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaaaa                                                                                          <  1:5017234/128‑1 (MQ=255)
                                   aaTTTAGTTTAACTTTAGCTTCTTCTGGTAAATAAAATATCTTTATATTAGTAACTGTTGTTGGAATATCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAac                                                                                         <  1:2416886/128‑1 (MQ=255)
                                     tttAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAacac                                                                                       >  2:6777378/1‑128 (MQ=255)
                                        aGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACtta                                                                                    <  1:3328798/128‑1 (MQ=255)
                                         gTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttat                                                                                   >  2:2519910/1‑128 (MQ=255)
                                          tttAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttatt                                                                                  <  2:556402/128‑1 (MQ=255)
                                              tcTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaa                                                                              <  2:7757811/127‑1 (MQ=255)
                                              aCTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaa                                                                              <  1:489316/128‑1 (MQ=255)
                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             <  1:1006222/128‑1 (MQ=255)
                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             <  2:5707950/128‑1 (MQ=255)
                                               cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             >  2:222863/1‑128 (MQ=255)
                                               cTTTAGCTTCTTATTGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             <  1:7095008/128‑1 (MQ=255)
                                                     cttcttcttGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTAtt                                                                       <  2:5111387/128‑1 (MQ=255)
                                                           ctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTTCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAatat                                                                 >  2:6122637/1‑128 (MQ=255)
                                                           ctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAatat                                                                 <  1:3817576/128‑1 (MQ=255)
                                                              gTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTTGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCa                                                              <  2:5859963/128‑1 (MQ=255)
                                                                aaaaaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCaaa                                                            <  1:607176/128‑1 (MQ=255)
                                                                  aaaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATg                                                          >  2:6434188/1‑128 (MQ=255)
                                                                     aaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAat                                                       <  2:3948691/128‑1 (MQ=255)
                                                                       atatCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAatat                                                     >  1:1308968/1‑128 (MQ=255)
                                                                          tCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAat                                                  >  1:6661608/1‑128 (MQ=255)
                                                                           cTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAata                                                      <  1:944003/123‑1 (MQ=255)
                                                                            tttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAatta                                                <  2:1230786/128‑1 (MQ=255)
                                                                             ttttATTAGTAACTGTTGTTGGAATTTCAACTATTTTTATTTCAGAAGATTTAGAAATTCAATCAATTTATTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATTAAAATGAATATAattat                                               >  2:3823012/1‑128 (MQ=255)
                                                                             ttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAattat                                               <  1:6175556/128‑1 (MQ=255)
                                                                                tattaGTAACTGTTGTTTGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCa                                            <  2:2681123/128‑1 (MQ=255)
                                                                                tattaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATTCAAATGAATATAATTATTCa                                            >  2:7061860/1‑128 (MQ=255)
                                                                                tattaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCa                                            >  2:7361178/1‑128 (MQ=255)
                                                                                  ttaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCaaa                                          >  1:7856851/1‑128 (MQ=255)
                                                                                    aGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAg                                        <  1:4096164/128‑1 (MQ=255)
                                                                                    aGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAg                                        <  1:534529/128‑1 (MQ=255)
                                                                                       aaCTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGgtg                                     <  2:3250301/128‑1 (MQ=255)
                                                                                       aaCTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAAATTCTTTTACTATTAAAAACACTTATAATAAAATTATTAAATATGAAAATGAATAAAATTATTCAAAATgtc                                     >  1:1470776/1‑127 (MQ=255)
                                                                                        tcTGTTGTTGGAATTTCATCTATGTTTATTTCAGAAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGgtgt                                    <  2:2967863/127‑1 (MQ=255)
                                                                                         cTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   <  1:3169191/128‑1 (MQ=255)
                                                                                          tgttgtTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGttt                                  <  1:4757958/128‑1 (MQ=255)
                                                                                           gttgttGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCGATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTc                                 >  1:6985122/1‑128 (MQ=255)
                                                                                           gttgttGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTc                                 <  2:1749464/128‑1 (MQ=255)
                                                                                           gttgatTGAATATCATCTATGTTTATTTCATCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAATGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTc                                 <  2:3642770/128‑1 (MQ=255)
                                                                                            ttgttgGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCa                                >  2:5294642/1‑128 (MQ=255)
                                                                                             tgttgGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCaa                               >  2:4541288/1‑128 (MQ=255)
                                                                                               ttgGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTc                                 >  1:1920748/1‑124 (MQ=255)
                                                                                                tgGAATTTCAACTATGATTATTTCAGCTGAGTTAGCAATTCCATCTATTGCTTTTACTATTAAAATCACTTATTATAAAAGTATTAAATATGCAAATGATTATAATTATTCAAAAGGTGTTTCTAAtt                            <  1:4344995/128‑1 (MQ=255)
                                                                                                aggAATATCAAATATGTTTATTTCAGCAGATTTAGCAATTCGATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGAATTAAATATGCAAATGAATATAATTATTCATTAGGTGTTTCAAAtt                            <  1:222949/127‑1 (MQ=255)
                                                                                                  gAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtct                          >  1:5950802/1‑128 (MQ=255)
                                                                                                  gAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtct                          <  2:4705232/128‑1 (MQ=255)
                                                                                                  gAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTAAAAGGTGTTTCAAATtctc                         >  1:851144/1‑128 (MQ=255)
                                                                                                  gAATTTCAACTATGTTTATTTCAGAAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtct                          <  2:4811621/128‑1 (MQ=255)
                                                                                                   aaTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtctc                         >  1:5822388/1‑128 (MQ=255)
                                                                                                   aaTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTCTTTCAAATtctc                         >  2:3512806/1‑128 (MQ=255)
                                                                                                    aTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAATGTGTTTCAAATTCTcc                        >  2:1617565/1‑128 (MQ=255)
                                                                                                    aTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTcc                        <  2:1493903/128‑1 (MQ=255)
                                                                                                     tttCATATATGTTTATTTCAGCAGATTTAGCAATTAAATAAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCt                       <  2:4962580/128‑1 (MQ=255)
                                                                                                     tttCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAATGTGTTTCAAATTCTCCt                       >  1:3685254/1‑128 (MQ=255)
                                                                                                     tttCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCt                       <  2:6619318/128‑1 (MQ=255)
                                                                                                        cAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTc                                 >  1:6798962/1‑115 (MQ=255)
                                                                                                        cAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTa                    >  1:2534648/1‑128 (MQ=255)
                                                                                                        cAACTATGTTTATTTCAGCAGATTTAGCAATACCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTa                    >  1:6482281/1‑128 (MQ=255)
                                                                                                         aaCTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTaa                   <  1:744747/128‑1 (MQ=255)
                                                                                                          agtATGTTTATTTCAGCAGATTTAGCAATTCAATCAATTGATTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAAc                  <  1:3018304/126‑1 (MQ=255)
                                                                                                            tATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACaa                <  1:396127/128‑1 (MQ=255)
                                                                                                              tGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATACTCCTTTAACaaaa              >  1:448562/1‑128 (MQ=255)
                                                                                                               gTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAAc             <  2:137293/128‑1 (MQ=255)
                                                                                                               gTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCATTTAACAAAAc             >  1:1758085/1‑128 (MQ=255)
                                                                                                                  tATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAACCCaa          >  1:3444263/1‑128 (MQ=255)
                                                                                                                  tATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTATCGTTTAACAACTCCaa          >  1:1156381/1‑128 (MQ=255)
                                                                                                                   aTTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCttt                     >  1:4597693/1‑116 (MQ=255)
                                                                                                                   aTTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAAc         >  2:1911958/1‑128 (MQ=255)
                                                                                                                    tttCAGCAGATTTAGAAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACg        <  1:5497785/128‑1 (MQ=255)
                                                                                                                     ttCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGa       <  2:2064278/128‑1 (MQ=255)
                                                                                                                      tCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCGTTTAACAATACCatagat      >  1:5177851/1‑123 (MQ=255)
                                                                                                                       cagcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtc                           <  2:4378431/106‑1 (MQ=255)
                                                                                                                        agcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGatta    <  1:421776/128‑1 (MQ=255)
                                                                                                                          cagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGattaat  >  2:7482540/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
AAGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGGTTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGATTAAT  >  CP016816/242732‑242981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: