Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816311,1483.T57.2% 469.6 / inf 334intergenic (‑71/‑243)JCVSYN2_01300/JCVSYN2_01305hypothetical protein/30S ribosomal protein S21
Reads supporting (aligned to +/- strand):  ref base . (94/49);  new base T (89/102);  total (183/151)
Fisher's exact test for biased strand distribution p-value = 5.71e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-03

Only 100 of 646 total aligned reads displayed.

TGTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAATATACTACTTATTTACTAATAAAATCTATTTTTATTT  >  CP016816/311021‑311274
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 gTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATAcattc‑‑‑‑‑‑‑at‑‑‑‑‑‑‑‑t                                                                                                                                               >  1:1115284/1‑128 (MQ=255)
 gTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATAcattc‑‑‑‑‑‑‑at‑‑‑‑‑‑‑‑t                                                                                                                                               >  1:4141975/1‑128 (MQ=255)
 gTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATAcattc‑‑‑‑‑‑‑at‑‑‑‑‑‑‑‑t                                                                                                                                               >  1:1632822/1‑128 (MQ=255)
 gTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGATACATACGATTCAAAACATATATAACATAcattc‑‑‑‑‑‑‑tt‑‑‑‑‑‑‑‑t                                                                                                                                               >  2:3634405/1‑128 (MQ=255)
   aCCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑at                                                                                                                                         >  1:3924593/1‑128 (MQ=255)
   aCCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑at                                                                                                                                         >  1:1981988/1‑128 (MQ=255)
   aCCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTAGTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑at                                                                                                                                         >  1:2471217/1‑128 (MQ=255)
       gTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatat                                                                                                                                     >  1:4319583/1‑128 (MQ=255)
       gTTGAAATAACTAATCCAAATGAACTTAAAAATGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatat                                                                                                                                     <  1:692238/128‑1 (MQ=255)
        ttGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatata                                                                                                                                    >  2:3680105/1‑128 (MQ=255)
          gTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCATAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatata                                                                                                                                  >  2:651749/1‑128 (MQ=255)
          gTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatata                                                                                                                                  >  2:147218/1‑128 (MQ=255)
          gTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatata                                                                                                                                  >  2:2434077/1‑128 (MQ=255)
          gTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatata                                                                                                                                  >  1:3558634/1‑128 (MQ=255)
           taataaCTAATCCAAATGAACTTAAAATTCAAATTAACTTTTTCATTTTACACACCCCTTATTAAAATATAAAATACTTGTTACATACGATTCAAAACATATATAACAAACAATC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatagat                                                                                                                                 >  2:4015427/1‑128 (MQ=255)
            aataaCTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatata                                                                                                                                >  1:3490020/1‑128 (MQ=255)
             ataaCAAATCTAAATGAAATTAAAAGTTTAATTAACTTTTTAATTTTACACACCCCTTATTAAAAAATAAAATAATTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatat                                                                                                                               <  2:2916749/128‑1 (MQ=255)
               aaCTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatat                                                                                                                             >  2:3179870/1‑128 (MQ=255)
               aaCTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatat                                                                                                                             >  2:4180671/1‑128 (MQ=255)
                 atAAACCAAATGAACATAAAATTTTAATTAACATTTTCATATTACACACACCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AA‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatat                                                                                                                           <  2:1226878/127‑1 (MQ=255)
                  tAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatata                                                                                                                          >  2:3823899/1‑128 (MQ=255)
                    aTCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatata                                                                                                                        >  1:3093462/1‑128 (MQ=255)
                    aTCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatata                                                                                                                        >  2:1430335/1‑128 (MQ=255)
                     tacataagAAATTAAAATAGTAATTAACAATATAATTTTACACACACATAATTAAAAAATAAAATACTTGATACATACGAATCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatat                                                                                                                       <  2:3095080/121‑1 (MQ=255)
                     taaatattaaattAAAATTGTAATTAAATTTTTTATTTTAAACACATATTATTAAAAAATAAAATAATTGTTAAATAATATTAAAAAAATATATAAAATACATTA‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatat                                                                                                                       <  1:751103/117‑1 (MQ=255)
                     tCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatat                                                                                                                       >  2:2264319/1‑128 (MQ=255)
                      ccAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatata                                                                                                                      >  2:1746000/1‑128 (MQ=255)
                      ccAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AA‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatata                                                                                                                      >  2:2320310/1‑128 (MQ=255)
                       cAAATTAACTAAAAATATTAATTAACTATTTCATTTTAAACACACCATATAAAAAAATAAAAAACTAGTTACATACTATTAAAAACATATATAACAAACATAC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatat                                                                                                                     <  2:4522322/128‑1 (MQ=255)
                       cAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatat                                                                                                                     >  1:3844418/1‑128 (MQ=255)
                         aaTGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat                                                                                                                   >  2:608371/1‑128 (MQ=255)
                          aTGAACTTAAAAATGTAATTAACTTTTTAATTTTACACACCCCATAATAAAAAATAAAATACTTGTTACATACGATTCAAAACATATAAAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑a                                                                                                                <  2:787777/128‑1 (MQ=255)
                           ttatattAAAATTTTAATTTAATTTTTATTTTTAAACACAAATTATTAAAAAATAAAATATTTGTTATATACTATTTAAAAAATATATAACATATATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑at                                                                                                               <  1:2756524/123‑2 (MQ=255)
                           tGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑at                                                                                                               >  1:2718335/1‑127 (MQ=255)
                           tGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAAAATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑at                                                                                                               >  1:3416182/1‑127 (MQ=255)
                           tGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAAGACTTGTTACATACGATTCAAAACATATATCACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑ag                                                                                                               >  1:4448558/1‑127 (MQ=255)
                            gAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAATC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑ata                                                                                                              >  2:3482884/1‑126 (MQ=255)
                              aatttataTTTTAATTAAATTTTTTTTTTTAAACAAAAATTATTAAATAATAAAATTGTTGTTACATAATATTAAAAAAATATATAAAATATATTT‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑a‑‑‑‑atatatatatatatatatatatat‑‑atata                                                                                                            <  1:4530752/121‑5 (MQ=255)
                              aCTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑ataaa                                                                                                            >  2:1043341/1‑124 (MQ=255)
                              aCTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATAAGATTCAAAACATATATAAAATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atata                                                                                                            <  1:3705547/128‑5 (MQ=255)
                               cTTAAAATTTTATTTATATTTTTTTTTTTAAACACCCCTTATTATAAAATAAAATACTTGTTAAATACGATTCAAAAAATATATAACATACATTA‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atatat                                                                                                           <  1:3966870/128‑6 (MQ=37)
                               cTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atatat                                                                                                           >  2:1187939/1‑123 (MQ=255)
                                ttAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atataat                                                                                                          >  2:4320819/1‑122 (MQ=255)
                                 tAAAATTTTAATTAAATATTTTATTTTACACACATCTTATTATAAAATAAAATAATTTTTACATAAGATTCAAAACATATATAACATAAATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atataatg                                                                                                         <  1:1360092/128‑8 (MQ=255)
                                   aaaTTGTAATTATATATTTCATTTTAAAAACCCCTTATTAAAAAAAAAAATAAATTTTATATAATATTCAAAAAATATATATAATAAATTA‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atataatgaa                                                                                                       <  1:4365342/128‑10 (MQ=16)
                                   aaaTTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑atatataatg                                                                                                       >  2:3268543/1‑119 (MQ=255)
                                     aTTGTAATTAACTTTTTCATTTAACTCACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACAATC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑‑‑‑‑TATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTaa                                                                                         <  2:1102357/128‑1 (MQ=255)
                                      ttGTTATTAACTTTTTATTTTTAAAAACAATTTATTAAAAAATAAAATAGTTGTTAGATAAGATTTAAAAAATATATATCATAAATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑ataatgaatgtat                                                                                                    <  1:375605/128‑13 (MQ=16)
                                      ttGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATAAATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATataaat‑‑a‑‑‑aataaataaata                                                                                                 >  2:2691570/1‑117 (MQ=255)
                                                cTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑AAA‑‑‑ATATTTAAATTaaaa                                                                                  >  1:1207539/1‑128 (MQ=255)
                                                   tttAATTTTACACACAACTTATTAAAAAATAAAATACTTGTTACATAAGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAat                                                                                 <  2:1673017/128‑1 (MQ=255)
                                                         tttACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑TCA‑‑‑ATATCTAAATTAAAatatatta                                                                           >  2:1933218/1‑124 (MQ=255)
                                                           taaacaaaacTTATTAAAAAATAAAATACTTTTTATATATTATTCAAAAAATATATATAATATATTA‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAg                                                                     <  2:3920499/119‑1 (MQ=37)
                                                             cacacaACTTATTAAAAAATATAATAATTTTTACATACTATTCAAAAAATATATAACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTataat                                                                       <  2:2795934/128‑1 (MQ=255)
                                                              acacacataattaAAAAATAAAATAATTGTTACATAATATTCAAAAAATATATAACATACATTCAATATATATA‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGtat                                                                          <  2:376429/119‑1 (MQ=255)
                                                              acacaaattattaAAAAATAAAATAATTGTTACATAATATTAAAAAAATATATAAAATAAATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTataa                                                                        <  1:1698721/121‑1 (MQ=255)
                                                                actacTTATTAAAAAATAAAATATTTTTTAGATATTATTAAAAAAATATATAACATAAATTA‑‑‑‑‑‑‑ATTATATATAT‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTataa                                                                        <  1:1458218/124‑1 (MQ=16)
                                                                   ttatataaAAAAATAAAATTTTTTTTATATTATTTTAAAAATATATAAAATATATATATA‑‑‑‑‑‑‑ATA‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCg                                                               <  2:3902187/125‑1 (MQ=255)
                                                                     ttattaAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑TCA‑‑‑ATATTTATATGAAAATATGTTATTATATCAAcag                                                               >  1:636038/1‑126 (MQ=255)
                                                                      tattaAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCT‑‑‑ATATGTAAATTAAAATATGTTATTATAGCaaaa                                                                >  2:502009/1‑126 (MQ=255)
                                                                      tattaAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑TCA‑‑‑ATATTAAAATAAAAATATTATATAATATCAAcata                                                              >  2:1856357/1‑125 (MQ=255)
                                                                        ttaAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATAAAAATATTGTATAATATCAAcaat                                                              >  2:4423992/1‑125 (MQ=255)
                                                                          aaaaaaTAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑AT‑‑ATAAAAAA‑‑‑ATATGTAAATGAAAATATTTTATTATAGAAAACGTTtat                                                          >  1:3344487/1‑128 (MQ=255)
                                                                            aaaatataatAATTTTTAAATAAGATTCAAAAAATATATAACAAAAATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTtat                                                          <  2:3361883/128‑1 (MQ=255)
                                                                               ataaaataCTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑t‑‑‑‑atatatatatatatatatatatat‑‑a                                                                                                                >  1:798099/1‑75 (MQ=255)
                                                                                 ataataatTGTTTTATACTATTCAAAACATATATAACATAAATTA‑‑‑‑‑‑‑TTTTTA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTaa                                                   <  1:2132187/126‑1 (MQ=255)
                                                                                 aaaataCTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATTAAAATATTTTATAATAGCAACCGTTTATAAAGTaaaa                                                 >  1:567218/1‑128 (MQ=255)
                                                                                 aaaataATTTTTAAATAATATTCAAAAAATATATAACATACATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGt                                                     <  2:3972928/128‑1 (MQ=255)
                                                                                  aaataCTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑TAA‑‑‑ATATGTAAAATAAAATATTTTATAATAGAAACATTTTATAAATTa                                                    >  1:46327/1‑128 (MQ=19)
                                                                                  aaataATTGTTACATACGATTAAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTaaaaaaa                                              <  1:179007/128‑1 (MQ=255)
                                                                                      aCTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GAA‑‑‑ATCTTAAAATAAAAATATTATAATATAGAAAGATTATATAAAGTaaaaaaa                                              >  2:1975382/1‑128 (MQ=16)
                                                                                       cTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑TCT‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAAACGTTTATAAAGTaaaaat                                               >  1:519595/1‑127 (MQ=255)
                                                                                       cTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATTGTATTATAGCAAACTTTTATATAGTAAAAAAATAg                                           >  1:1588778/1‑128 (MQ=255)
                                                                                       cTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑ATATATAAAAAA‑‑‑ATATTTAAATGAAAATATTTTATAATAGAAACAATTTATAAATTaaaaaa                                               >  1:2309411/1‑128 (MQ=255)
                                                                                        ttGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑ATATATAAATCA‑‑‑ATATGTAAATGAAAATATGATATAATAGAAAAATTTTATAAATaaaaaaaa                                              >  1:1923720/1‑128 (MQ=255)
                                                                                         tGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GAA‑‑‑ATATTTAAATGAAAATATGTTATAATAGCAAAAGTTTATAAATTAAAAAAAt                                             >  1:2186214/1‑128 (MQ=25)
                                                                                         tGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GAA‑‑‑ATATGTATATTAAAATATGTTTTATTATAAACAGTTTATATAATAAAAAtatat                                           >  1:898780/1‑127 (MQ=19)
                                                                                          gTTACATAATATTCTAAAAATATATAAAATACATTCTTT‑‑‑‑ATA‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGt                                          <  2:1622711/128‑1 (MQ=255)
                                                                                            tACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑ATATAT‑‑‑AAATATATATTTAAAAGAAAATATTGTATAATAGAAACAGTTTATAATTTAAAAAAATaat                                          >  1:2556159/1‑126 (MQ=255)
                                                                                             agaTACTATTAAAAAAATATATAACATACATTA‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTa                                         <  1:3629639/126‑1 (MQ=25)
                                                                                              cATACGATTCAAAACATATATAGCATACATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑TAA‑‑‑ATATATAAAATAAAATATTTTATAATATCAAACTTTTATAAATAAAAAAAATAGTaa                                        >  1:100063/1‑128 (MQ=16)
                                                                                                taagATTAAAAACATATATAACATAAATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATTTGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAat                                      <  2:2615936/125‑1 (MQ=255)
                                                                                                tACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATATAAA‑‑‑AT‑‑‑TAA‑‑‑ATATATAAAAAAAAATATATTATAATATAAAAAGTTTATAAAATAAAAAAATATTAAAaataa                                  >  2:708433/1‑127 (MQ=255)
                                                                                                 atcaTTAAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑TATAAATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAAtata                                   <  1:1283109/125‑1 (MQ=255)
                                                                                                 aCGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAAtata                                   <  2:3538599/128‑1 (MQ=255)
                                                                                                  cGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GAA‑‑‑ATATTTAAATTAAAATATGTTATAATAGCAACAGTATATAAATTAAAAAAATATTAAATAtacaa                                >  2:4085834/1‑126 (MQ=37)
                                                                                                  cGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑AT‑‑ACT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGTTATATTAGAAAACGTTTATAAATAAAAAAAATAGTATATataata                                >  1:2223403/1‑128 (MQ=255)
                                                                                                   gATTAAAAACATATATATCATACATTA‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAAtata                                   <  1:282889/128‑1 (MQ=37)
                                                                                                      tCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑AAA‑‑‑ATATTTAAATTAAAATATTTTATAATATCAAGATTTAATAAAATAAAAAAATAGtatataatcta                                >  2:3006825/1‑123 (MQ=255)
                                                                                                      tCAAAACATATATAACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑TAA‑‑‑ATATATATATTAAAATATTATATAATATAAAAAGTTTATAAATAAAAAAAATATAAAATataataat                              >  2:862168/1‑126 (MQ=12)
                                                                                                        aaaaCATATATAACATACATTC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑TCA‑‑‑ATATGTAAATTAAAATATTTTATAATATCAACAGTTTATAAATTAAAAAAATATTAAATATACTAattatt                          >  1:4235332/1‑128 (MQ=255)
                                                                                                         aaaCATATATAACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑TCA‑‑‑ATATTTAAATGAAAATATTTTATAATATCAACCTTTTATAAATTAAAAAAATATTAAATATACTAATTAt                           >  1:4022519/1‑128 (MQ=255)
                                                                                                           aCATATATAACATACATTC‑‑‑‑‑‑‑ATTATATA‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑TAA‑‑‑ATATTTATATTAAAATATTTTATAATATAAAAAGATTATAAATTAAAAAAATATTAAATATAATAATTaa                           >  2:1552395/1‑127 (MQ=12)
                                                                                                            tatataaAACATATATTA‑‑‑‑‑‑‑TA‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑TT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAATATACTACTTATTTACtaa                    <  2:1332996/127‑1 (MQ=255)
                                                                                                            cATATATAACATACATTC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATTAAAATATTGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAATATAATACTTATTTaat                      >  1:3229542/1‑126 (MQ=255)
                                                                                                              tatataACATACATTC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AA‑‑‑TAA‑‑‑ATATTTAAATTAAAATATGTTATAATATCAACCGTTTATAAAGTAAAAAAATATAAAATATAAAAATTATTtaataa                    >  1:3028109/1‑128 (MQ=255)
                                                                                                               atataACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑AT‑‑ATAAATCA‑‑‑ATATTTAAAATAAAATATTTTATAATAGAAACATTTTATAAATAAAAAAAATAATAAATATAATAattattaaata                     >  1:407852/1‑123 (MQ=255)
                                                                                                                tataACATACATTC‑‑‑‑‑‑‑ATTATA‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAATATACTACTTATTTACtaa                    <  2:2228030/128‑1 (MQ=255)
                                                                                                                tataACATACATTC‑‑‑‑‑‑‑ATTA‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGAAAATGAAAATATTTTATAATAGAAACATTTTATAAAATAAAAAAATAGTAAATAAACTACTTATTtaataaaa                  >  1:2672144/1‑126 (MQ=255)
                                                                                                                 ataACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑AT‑‑ATAAATCA‑‑‑ATATGTAAATTAAAATATGTTATAATAGAAACATTTTATAAAGTAAAAAAATAATAAATATACTAATTATTTACtaat                   >  2:2098071/1‑128 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
TGTACCAGTTGTAATAACTAATCCAAATGAACTTAAAATTGTAATTAACTTTTTCATTTTACACACCCCTTATTAAAAAATAAAATACTTGTTACATACGATTCAAAACATATATAACATACATTC‑‑‑‑‑‑‑AT‑‑‑‑‑‑‑‑T‑‑‑‑ATATATATATATATATATATATAT‑‑A‑‑‑AT‑‑‑GCA‑‑‑ATATGTAAATGAAAATATGGTATAATAGCAACCGTTTATAAAGTAAAAAAATAGTAAATATACTACTTATTTACTAATAAAATCTATTTTTATTT  >  CP016816/311021‑311274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: