Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 478,738 C→A G301V (GGT→GTT)  JCVSYN2_02210 ← DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816478,7380CA98.9% 5612.3 / NA 1953G301V (GGT→GTT) JCVSYN2_02210DNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (6/16);  new base A (1010/906);  total (1020/933)
Fisher's exact test for biased strand distribution p-value = 1.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2001 total aligned reads displayed.

TGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAAT  >  CP016816/478612‑478863
                                                                                                                               |                                                                                                                             
tGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATTATTCTTCTATATAAATCATTAAGGTCAGAAGTATTAAATCTaca                                                                                                                              >  1:4075360/1‑127 (MQ=255)
  tAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTaacac                                                                                                                            >  2:1719782/1‑128 (MQ=255)
     cATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCAt                                                                                                                         <  1:2284538/128‑1 (MQ=255)
     aatccGTTTTTCATTATTTAATATAATTGTTTGTTGATTCATTTATTTATATTTTTTTAATATTTATTTTCTAATAATGATTCTTCTATATAAATCATTAATTTAAGAAGTTGTAAATGTAACACCAt                                                                                                                         <  1:3014067/124‑1 (MQ=255)
       tACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCt                                                                                                                       <  1:4309759/128‑1 (MQ=255)
         cGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCATAAGTAGTAAATCTAACACCATCTaa                                                                                                                     >  2:1372881/1‑128 (MQ=255)
             tttCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGa                                                                                                                 <  1:506532/128‑1 (MQ=255)
             tttCATTATTTACAATAATTGATGGTGCACCAATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAAACATTAATTTCAGAAGTAGTAAATCTAACACCATCTAAttca                                                                                                                 >  1:2870183/1‑126 (MQ=255)
                    attTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTg                                                                                                          >  2:114928/1‑128 (MQ=255)
                    attTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTg                                                                                                          <  2:3100909/128‑1 (MQ=255)
                    attTACAAAAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCCAACACCATCTAATTGAATAATTg                                                                                                          >  1:1553649/1‑128 (MQ=255)
                     tttACAATAATTGATGGTGTACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                         <  2:4487067/128‑1 (MQ=255)
                     tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATACATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                         >  1:245260/1‑128 (MQ=255)
                     tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                         >  1:2551687/1‑128 (MQ=255)
                     tttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTgg                                                                                                         >  2:3219220/1‑128 (MQ=255)
                      ttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGa                                                                                                        >  1:3849721/1‑128 (MQ=255)
                      ttACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTAATTTATAATAATCATTCATCTATAAAAATAATTAATTTCATAAATAGTAAATCTAAAAACATCTAATTCaataaattta                                                                                                        >  2:439124/1‑123 (MQ=255)
                      ttACAATAATAGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTACAGAAGAAGTAAATCTAACACCATCTAAATGAATAATTGGa                                                                                                        >  2:1310470/1‑128 (MQ=255)
                        aCAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACg                                                                                                      >  1:3270209/1‑128 (MQ=255)
                        aCAATAAATGAAGGTGCAAACATATCTTTAACTTTTTTTAATATTTCATTTATAAAAATTATTATTATATAAAAATCATTAATTTCAGAATTAGTAAATCTAACCCAATCTAATTGAATAAttctaag                                                                                                      >  1:842008/1‑123 (MQ=255)
                         cAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATATATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAAGTGAATAATTGGAAGa                                                                                                     >  1:1900552/1‑128 (MQ=255)
                          aataatTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGaa                                                                                                    >  1:58980/1‑128 (MQ=255)
                               ttGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCt                                                                                               >  1:1210270/1‑128 (MQ=255)
                                tGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTTAATAATTGGACGAATGTCtg                                                                                              >  2:2830032/1‑128 (MQ=255)
                                  aTGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCtggt                                                                                            <  1:3757811/128‑1 (MQ=255)
                                   tGGTGCAACCATTTCTTTAACTTTTTTTAATCTTTCATTTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGTACGAATGTCtggt                                                                                            >  2:629368/1‑128 (MQ=255)
                                     gTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGa                                                                                         >  2:2739861/1‑128 (MQ=255)
                                       gcaacaaTTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAAt                                                                                       <  1:1624634/122‑1 (MQ=255)
                                         aCCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATaa                                                                                     <  2:3319915/128‑1 (MQ=255)
                                         aCCCATTTCTTTAAATTTTTTAAATCTTTCTTTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGAAAATCAAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATaa                                                                                     <  1:2360994/128‑1 (MQ=255)
                                           ccAATTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACt                                                                                   <  2:1100177/128‑1 (MQ=255)
                                           acATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACt                                                                                   <  2:1615893/127‑1 (MQ=255)
                                               ttCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGaa                                                                               <  2:3768087/128‑1 (MQ=255)
                                               ttCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATGCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGaa                                                                               <  2:2926727/128‑1 (MQ=255)
                                                   ttAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGATTTAc                                                                           >  2:2656153/1‑128 (MQ=255)
                                                    tAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTAct                                                                          <  2:2108592/128‑1 (MQ=255)
                                                      aCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActct                                                                        <  2:3852004/128‑1 (MQ=255)
                                                      aCTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTActct                                                                        <  2:1993514/128‑1 (MQ=255)
                                                       cTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGaata                                                                                                              <  2:4532385/89‑1 (MQ=255)
                                                       cTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACtctc                                                                       <  2:721283/128‑1 (MQ=255)
                                                        tttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                      <  2:1253363/128‑1 (MQ=255)
                                                        tttttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                      <  2:3441303/128‑1 (MQ=255)
                                                          gtggtaagcgttCATTTCTCATAAGTTTTCTGCTATATAAATCATTAATTTCTGAAGTAGTAAAGCAAACACCATCTAATGGAATAATTGGACTAATGGCTGGTGGAATCACTGGAATTACTCTCATa                                                                    <  1:790497/118‑1 (MQ=255)
                                                           ttttAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATTACTGGAATTACTCTCATaa                                                                   >  1:2211853/1‑128 (MQ=255)
                                                           ttttAATATTTCATTTCTAATAATGATTCTTATATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATGTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTTGAATTACTCTCATaa                                                                   <  2:3521979/128‑1 (MQ=255)
                                                            ttttATCTTTGATTTCTAATAATGATTCTTCAATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTATTTGAATAATTGGACGAATGTCTGGCGGAATAACTGGAATTACTCTCATAAc                                                                  <  1:733885/128‑1 (MQ=255)
                                                              tAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACca                                                                <  2:4085915/128‑1 (MQ=255)
                                                              tAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACca                                                                <  1:66368/128‑1 (MQ=255)
                                                              tAATCTTTAATTTCTAATATTGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACca                                                                <  1:4010160/128‑1 (MQ=255)
                                                               aaTCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACtctc                                                                       >  2:4021329/1‑120 (MQ=255)
                                                                 tCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGTTGGAATAACTGGAATTACTCTCATAACcattc                                                             >  1:1799234/1‑128 (MQ=255)
                                                                  cTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattca                                                            <  2:3160660/128‑1 (MQ=255)
                                                                  cTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattca                                                            >  1:3945378/1‑128 (MQ=255)
                                                                   tttCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACcattcat                                                           <  2:3660957/128‑1 (MQ=255)
                                                                     tCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATATAATTGAATATTTGGCCGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCattcattc                                                         <  2:1931201/128‑1 (MQ=255)
                                                                       aTTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAg                                                       <  1:3615155/128‑1 (MQ=255)
                                                                       aTTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAg                                                       >  1:1126520/1‑128 (MQ=255)
                                                                       aTTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAg                                                       <  1:1361712/128‑1 (MQ=255)
                                                                          ttCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGa                                                     >  2:3933284/1‑128 (MQ=255)
                                                                             taataaTGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGt                                                  <  2:1515052/128‑1 (MQ=255)
                                                                              aataatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGtt                                                 >  2:2194636/1‑128 (MQ=255)
                                                                                taatGATTCTTCTATATAAATCATTAAATTCAGAAGTAGTAACTCTAACCCCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTgg                                               <  1:3113205/128‑1 (MQ=255)
                                                                                 aatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                              <  1:3186314/128‑1 (MQ=255)
                                                                                 aatGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACCTTGGt                                              >  1:999121/1‑128 (MQ=255)
                                                                                 aatGATTCTGCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGt                                              <  1:2780494/128‑1 (MQ=255)
                                                                                  atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTgggt                                             >  2:1440437/1‑126 (MQ=255)
                                                                                  atGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGtt                                             >  1:4163994/1‑128 (MQ=255)
                                                                                   tGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCa                                                                      >  1:2007313/1‑102 (MQ=255)
                                                                                   tGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTa                                            <  1:1000185/128‑1 (MQ=255)
                                                                                      ttcttcTATATAAATCATTAATTTCAGAAGTAGTAAATCTCACACCATCTAATTGACTAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGaa                                         <  2:871651/128‑1 (MQ=255)
                                                                                       tcttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAAc                                        >  2:567435/1‑128 (MQ=255)
                                                                                         ttctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGt                                      <  1:1276915/128‑1 (MQ=255)
                                                                                          tctATATAAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGtt                                     <  1:1719782/128‑1 (MQ=255)
                                                                                             atataAATCAATAATTTCAGAAGTAGTAAATCTAACAACATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTa                                  <  2:1822724/128‑1 (MQ=255)
                                                                                               ataAATCTATAAGTTCAGAAGTAGTAATTCTAACACCATCTAATTGAATAATTGGTCGAATGTCTGGTGGATTAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaaa                                <  1:2120757/128‑1 (MQ=255)
                                                                                               ataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATTACTGGAATTACTCTCATTCCCATTCATACAGGACGATGGTAAGCACGTGTTaac                                >  1:2941667/1‑127 (MQ=255)
                                                                                               ataAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTaaa                                >  2:2489279/1‑128 (MQ=255)
                                                                                                tataTCATTTATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAg                               <  2:2276996/125‑1 (MQ=255)
                                                                                                taAATCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAg                               <  1:3674976/128‑1 (MQ=255)
                                                                                                    tCATTAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAg                               >  1:3540122/1‑124 (MQ=255)
                                                                                                        tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                       <  1:4541382/128‑1 (MQ=255)
                                                                                                        tAATTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCaaaa                       <  2:4234650/128‑1 (MQ=255)
                                                                                                         aaTTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAg                      >  2:358644/1‑128 (MQ=255)
                                                                                                         aaTTTCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAg                      <  1:439126/128‑1 (MQ=255)
                                                                                                          aTTTGAGAAGTAGTACATCTAACACCATCTAATGGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGt                     <  2:2996541/128‑1 (MQ=255)
                                                                                                           tttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGtt                    >  2:4149003/1‑128 (MQ=255)
                                                                                                            ttCAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGttt                   >  2:930121/1‑128 (MQ=255)
                                                                                                              cAGAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGAAGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCt                 <  2:2577201/128‑1 (MQ=255)
                                                                                                                gAAGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGAATCAAAAGTATCTaa               >  1:144226/1‑128 (MQ=255)
                                                                                                                 aaGTAGTAAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGAGGAATAACTGGAATTCCTCTCATAACCATTCATTCAGGACGTTGGTTAGCACGTTTTAAAGCATCAAAAGTTTCTaaa              >  1:3461605/1‑128 (MQ=255)
                                                                                                                    tagtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTATCTAAACGt           >  1:4470890/1‑128 (MQ=255)
                                                                                                                     agtaAATCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtt          >  2:477026/1‑128 (MQ=255)
                                                                                                                     agtaAATCTAACAACATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGtt          <  2:794664/128‑1 (MQ=255)
                                                                                                                        aaaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTta       >  1:933068/1‑128 (MQ=255)
                                                                                                                        aaaTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTta       >  1:2656768/1‑128 (MQ=255)
                                                                                                                         aagcTAACACCATCTAATTGAATAATTGGACGAATTTCTGGGGGAATAATTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaa      <  1:4231160/125‑1 (MQ=255)
                                                                                                                          ctCTAACACCATCTAAATGAATAAGTGGACGACTGTCTGGTGGAATAACTGGAATTACTCTCATAACCCTTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaat     <  1:1065619/127‑1 (MQ=255)
                                                                                                                          aTCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTtaat     >  2:4306543/1‑128 (MQ=255)
                                                                                                                           tCTAACACCATCTAATTGAATAATTGGACGAATGTCTGGAGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGACCGATTTAAAGCATCAAAAGTATCTAAACGTATtaata    >  1:3357065/1‑128 (MQ=255)
                                                                                                                            cTAACACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTAATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGATACTAAACTTTTtaataa   >  2:2344531/1‑128 (MQ=255)
                                                                                                                               |                                                                                                                             
TGTAACATACGTTTTTCATTATTTACAATAATTGATGGTGCACCCATTTCTTTAACTTTTTTTAATCTTTCATTTCTAATAATGATTCTTCTATATAAATCATTAATTTCAGAAGTAGTAAATCTACCACCATCTAATTGAATAATTGGACGAATGTCTGGTGGAATAACTGGAATTACTCTCATAACCATTCATTCAGGACGTTGGTTAGAACGTTTTAAAGCATCAAAAGTTTCTAAACGTTTTAATAAT  >  CP016816/478612‑478863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: