Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,791 T→A intergenic (‑100/+71) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7910TA97.6% 6119.2 / 36.7 2078intergenic (‑100/+71)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base T (25/24);  new base A (990/1039);  total (1015/1063)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2160 total aligned reads displayed.

ATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484665‑484913
                                                                                                                              |                                                                                                                           
        aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                    >  1:1866119/1‑128 (MQ=255)
        aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                    >  1:3609830/1‑128 (MQ=255)
        aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                    >  2:4152721/1‑128 (MQ=255)
          aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                  >  2:520855/1‑126 (MQ=255)
            aTAGTCAAAACTCATAGATTTAATACTATTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCaa                                                                                                                >  1:736090/1‑128 (MQ=255)
             tAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                               >  2:3454883/1‑128 (MQ=255)
             gatataaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGTTCATAGCAGAATCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                               <  1:3357642/123‑1 (MQ=255)
              agtataaaataaTAGATTTAATAGTTTTATATTTTACCAATAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                              <  1:3254318/117‑1 (MQ=255)
              aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                              >  1:4532859/1‑128 (MQ=255)
                  aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGAATATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                          >  2:1448540/1‑128 (MQ=255)
                  aaaaCCCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                          >  1:1257622/1‑128 (MQ=255)
                    aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                        >  1:2908734/1‑128 (MQ=255)
                    aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                        >  1:2192154/1‑128 (MQ=255)
                      cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                      >  1:1469861/1‑128 (MQ=255)
                          tAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                  <  1:1173872/128‑1 (MQ=255)
                              tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACAACAAAACTc                                                                                              >  1:2577929/1‑128 (MQ=255)
                              tttAATACTTTAATAGTTTACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                              <  2:2907451/128‑1 (MQ=255)
                                tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                            >  2:745796/1‑128 (MQ=255)
                                tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATAAAACACACAAAAACTCAc                                                                                            >  2:2814768/1‑128 (MQ=255)
                                tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGAAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAAATCaa                                                                                            >  2:2142985/1‑127 (MQ=255)
                                     cTTTTATAGTTGACCAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                      <  2:2629930/128‑1 (MQ=255)
                                        ttATATTAGACCAAAAAATAAGATAGATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTAtc                                                                                    <  2:4075420/128‑2 (MQ=255)
                                        ttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGCAGCTCTTAACAACTAAACTACACACTATTATTTAATTATATATTTTTTTAATATAAAAAGCAAAAAAACACACAAAAACTCACACATTATg                                                                                   >  2:1775105/1‑128 (MQ=255)
                                          ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                 <  1:392483/128‑1 (MQ=255)
                                           taGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTg                                                                                >  2:1248775/1‑128 (MQ=255)
                                            aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                               >  1:3524730/1‑128 (MQ=255)
                                               tGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGtt                                                                            <  1:1313575/128‑1 (MQ=255)
                                                  ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATAATTTAATTATATTTTTTTATAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                         <  2:1113228/128‑1 (MQ=255)
                                                  acAAAAAATTAGATAGAGCATAGCAGAAGATCTTATCTACTTAACTACACTAGATTTTTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTaa                                                                         <  1:1517962/127‑1 (MQ=255)
                                                   cAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaa                                                                        <  2:1047835/128‑1 (MQ=255)
                                                     aaaaaTAAGATAGATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  1:3785511/128‑1 (MQ=255)
                                                     aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  2:1691689/128‑1 (MQ=255)
                                                     aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACAATCGATTTTTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACTCACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  1:940128/128‑1 (MQ=255)
                                                       taaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  1:2473494/127‑1 (MQ=255)
                                                        aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatta                                                                   >  1:4106835/1‑128 (MQ=255)
                                                        aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAACAATTATGGTGAGATTATAAatta                                                                   >  2:252718/1‑128 (MQ=255)
                                                         aTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCATAATGGGTGAGtttttgtgtgtt                                                                  <  1:1073090/128‑10 (MQ=37)
                                                           aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                >  2:422094/1‑128 (MQ=255)
                                                           aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                >  1:273376/1‑128 (MQ=255)
                                                           aaGATAGAGCATAGCAGAAGCTCTTAACAACAAAACTACACACGATTATTTAATAATATTTTATTTTAATAAAAAAAGCAAATAAACACACAAAAACTAACCCATTATATTTAGTATAAAAattataa                                                                >  2:1852377/1‑126 (MQ=255)
                                                            aGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGTTGAGTTTAAAAATTATTCa                                                               >  2:3228058/1‑128 (MQ=255)
                                                               tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATAGTGATTTTAAAAAATATTCaaaa                                                            >  2:2275717/1‑128 (MQ=255)
                                                                tgagCATAGCAGAAGCTCTTAACATCTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATTATTCaaaaa                                                           <  1:4227342/127‑1 (MQ=255)
                                                                agatCATAGAAGAAGCTCTAAAAAACTAAACTACACTCGATTATTAAATTAAATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                           <  1:2835031/128‑1 (MQ=255)
                                                                 gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATTAAAAAAGCAATTAACCACCAAAAAACTCCCCAATTATGGTTCTTTTAAAAATAATACAAAAAt                                                          >  1:240544/1‑128 (MQ=255)
                                                                 gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          <  1:3184453/128‑1 (MQ=255)
                                                                 gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAAACATTATGGTGAGTTTAATAATTATTCaaaaaa                                                          >  2:3814565/1‑127 (MQ=255)
                                                                  agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACCAATTATGGTGAGTTTAAAAATTAATCATAACtt                                                         >  1:1001330/1‑128 (MQ=255)
                                                                  agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATAAAACACACAAAAAATCACCAATAATGTGAAGTTTAAAATTAATAAAAAAAtt                                                         >  2:2986769/1‑128 (MQ=255)
                                                                  agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATAAAAAACGCAATTAAACACAAAAAAACTACCGAATTATGTTTAATTTAAAAATATATCAAAAAtt                                                         >  1:2983187/1‑128 (MQ=255)
                                                                  agCATAGCAGAAGCTCTTAACAACTAAACTACACACGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         <  1:2648229/128‑1 (MQ=255)
                                                                      tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  1:2004486/128‑1 (MQ=255)
                                                                         cAGAAGCTCTTAACAACTAAACTACACTCGATAATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattat                                                  <  2:4320112/128‑1 (MQ=255)
                                                                           gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                <  2:2744935/128‑1 (MQ=255)
                                                                            aaGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattattat                                               >  2:1361654/1‑126 (MQ=255)
                                                                              gCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                             <  2:3927144/128‑1 (MQ=255)
                                                                                 ctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATAATTCAAAAATTTGATTATTTTAAATc                                          >  2:3381452/1‑128 (MQ=255)
                                                                                  ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                         <  1:3445107/128‑1 (MQ=255)
                                                                                     acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGTTGAGTTTAAAAATTATTCAAAACTATGATTATTTTAAATCAAtt                                      >  2:2356357/1‑128 (MQ=255)
                                                                                      caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                       <  2:3297687/94‑1 (MQ=255)
                                                                                       aactaaactaCACTCTATTATTTATTTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    <  1:1378565/128‑1 (MQ=255)
                                                                                        aataaactaAAATAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   <  1:3130487/126‑1 (MQ=255)
                                                                                         ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                          >  2:638689/1‑88 (MQ=255)
                                                                                         ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                  <  1:1625054/128‑1 (MQ=255)
                                                                                          taaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGc                                 <  2:3715307/128‑1 (MQ=255)
                                                                                           aaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCt                                <  1:3697637/128‑1 (MQ=255)
                                                                                            aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                               <  2:907848/128‑2 (MQ=255)
                                                                                            aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                               >  2:3996123/1‑128 (MQ=255)
                                                                                             actaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTcc                              <  2:1951865/128‑1 (MQ=255)
                                                                                              ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                             <  2:3079066/128‑1 (MQ=255)
                                                                                              ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                             >  1:4167856/1‑128 (MQ=255)
                                                                                                acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACGCAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAACTCAATTGAAGCTCCGGc                           >  1:4313295/1‑128 (MQ=255)
                                                                                                acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGACGCTCCAGc                           >  1:2282185/1‑128 (MQ=255)
                                                                                                acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           >  1:4374384/1‑128 (MQ=255)
                                                                                                acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           <  2:586091/128‑1 (MQ=255)
                                                                                                acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           <  1:4460958/128‑1 (MQ=255)
                                                                                                 cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                          >  1:1944215/1‑128 (MQ=255)
                                                                                                 cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                          <  1:509944/128‑1 (MQ=255)
                                                                                                  acTCGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                         <  2:1305559/128‑1 (MQ=255)
                                                                                                  acTCCATTATTTAATTATATATTTATTTAATAAAAAAATCAATTAAACACACAAAAACTAACCCATTATGCTGAGTTTAAAAATTATTAAAAAATATTAAAATTTTAAATCAATTTaacaaaaaactt                         >  1:1679308/1‑118 (MQ=255)
                                                                                                    tagATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                       <  1:4008294/126‑1 (MQ=255)
                                                                                                    tCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTGGAGCTTCa                       <  2:1177713/128‑1 (MQ=255)
                                                                                                    tCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                       <  1:860226/128‑1 (MQ=255)
                                                                                                     cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                      <  2:3633621/128‑1 (MQ=255)
                                                                                                      gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                     >  1:2072584/1‑128 (MQ=255)
                                                                                                      gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                     <  2:4487776/128‑1 (MQ=255)
                                                                                                       attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                    >  2:3173091/1‑128 (MQ=255)
                                                                                                       attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTAAAGCTACAGCTTCAACt                    >  2:4411764/1‑128 (MQ=255)
                                                                                                        ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTa                   >  1:593743/1‑128 (MQ=255)
                                                                                                          attTAATTATATTATTTTTTAAAATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATAATTAAAAAATTTGATTATTTTAAATCAATTGAAGCTACACCTTCAACTAAt                 >  2:4570611/1‑128 (MQ=255)
                                                                                                            ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt               <  2:1529641/128‑1 (MQ=255)
                                                                                                             tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa              <  2:835948/128‑1 (MQ=255)
                                                                                                              aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg             <  1:3064795/128‑1 (MQ=255)
                                                                                                                 tataTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCtt          <  2:1603836/128‑1 (MQ=255)
                                                                                                                  atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCATCTTCAACTAATTTAGCttt         >  2:2436396/1‑128 (MQ=255)
                                                                                                                  atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt               >  1:1786908/1‑122 (MQ=255)
                                                                                                                  atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  2:353864/1‑128 (MQ=255)
                                                                                                                     tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt      <  2:2041865/128‑1 (MQ=255)
                                                                                                                     attttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt      >  1:807669/2‑128 (MQ=255)
                                                                                                                         tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct  <  1:65916/128‑1 (MQ=255)
                                                                                                                              |                                                                                                                           
ATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484665‑484913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: