Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,793 A→T intergenic (‑102/+69) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7930AT97.9% 5907.3 / 31.5 2081intergenic (‑102/+69)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (25/19);  new base T (957/1080);  total (982/1099)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2158 total aligned reads displayed.

TATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484667‑484913
                                                                                                                              |                                                                                                                         
      aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                    >  1:1866119/1‑128 (MQ=255)
       aaaaTAGTCAAAACTCATAGATTAAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaag                                                                                                                   <  2:2354632/128‑2 (MQ=255)
        aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                  >  1:3922560/1‑126 (MQ=255)
        aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                  >  2:520855/1‑126 (MQ=255)
           tAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGGTTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                               <  1:732659/128‑1 (MQ=255)
            agtataaaataaTAGATTTAATAGTTTTATATTTTACCAATAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                              <  1:3254318/117‑1 (MQ=255)
              taaaaaCTCATAGATTTAATCCTTTTATAGTAGACCAAAAAATAAGATAGAGCATAGCAGAAGCTATTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                            <  2:1036564/126‑1 (MQ=255)
               cAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaaa                                                                                                           <  2:2139942/128‑1 (MQ=255)
                aaaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTTACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                          >  2:3610675/1‑128 (MQ=255)
                aaaaCCCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                          >  1:1257622/1‑128 (MQ=255)
                 aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                         >  2:1527672/1‑128 (MQ=255)
                  aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                        >  1:2908734/1‑128 (MQ=255)
                  aaCTCATAGATATAATCCATTTATAGTTGACCAAAAAATAAGATAGATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                        <  2:2040359/128‑1 (MQ=255)
                    cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacacac                                                                                                      >  1:1469861/1‑128 (MQ=255)
                        tAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaaaa                                                                                                  <  1:1173872/128‑1 (MQ=255)
                         aGATTTAATCCTTTTATAGTTGACCTAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTAAATTATATTTTTTTTTAATATATAAAGCAATTAAACACACaagaa                                                                                                 <  2:2806798/128‑1 (MQ=255)
                            tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACAACAAAACTc                                                                                              >  1:2577929/1‑128 (MQ=255)
                            tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                             >  1:3444460/1‑128 (MQ=255)
                              tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACACCTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                            >  1:4102529/1‑128 (MQ=255)
                              tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                            >  2:745796/1‑128 (MQ=255)
                                     tttATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTACACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTAt                                                                                     >  2:380567/1‑128 (MQ=255)
                                       tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATAATATTTTTTTTTAATATAAAAACAAATTAAACACACAAAAACTcccctttggtga                                                                                   >  1:1376001/1‑120 (MQ=255)
                                        ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                 >  1:593526/1‑128 (MQ=255)
                                        ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                 <  1:859322/128‑1 (MQ=255)
                                         taGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                       <  2:1597823/122‑1 (MQ=255)
                                          aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                               >  1:2981511/1‑128 (MQ=255)
                                            ttGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTTAGt                                                                             >  2:297808/1‑128 (MQ=255)
                                             tGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGtt                                                                            <  1:1313575/128‑1 (MQ=255)
                                                acAAAAAATTAGATAGAGCATAGCAGAAGATCTTATCTACTTAACTACACTAGATTTTTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTaa                                                                         <  1:1517962/127‑1 (MQ=255)
                                                  aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGATTaaaa                                                                       >  2:3072689/1‑128 (MQ=255)
                                                   aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  1:3604115/128‑1 (MQ=255)
                                                   aaaaaTAAGATAGAGAATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  2:2266786/128‑1 (MQ=255)
                                                    aaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAat                                                                     <  2:3294028/128‑1 (MQ=255)
                                                    aaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAat                                                                     <  2:1442969/128‑1 (MQ=255)
                                                     taaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  1:2473494/127‑1 (MQ=255)
                                                     aaaTAAGATATATCATAGCAGTAGCTCTTAACAACTAAACTACACTCGAATATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  2:1110165/128‑1 (MQ=255)
                                                     aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  2:1771389/128‑1 (MQ=255)
                                                       aTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCATAATGGGTGAGtttttgtgtgtt                                                                  <  1:1073090/128‑10 (MQ=37)
                                                         aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                >  1:273376/1‑128 (MQ=255)
                                                         aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                <  1:4458340/128‑1 (MQ=255)
                                                             tAGAGAATAGCAGAAGCTCTTAAAAAATAAACTAGAATCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaa                                                            <  1:3351747/128‑1 (MQ=255)
                                                               gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaaa                                                          >  1:2829078/1‑127 (MQ=255)
                                                               gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          >  1:3522421/1‑128 (MQ=255)
                                                               gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          <  1:4525108/128‑1 (MQ=255)
                                                                agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACCAATTATGGTGAGTTTAAAAATTAATCATAACtt                                                         >  1:1001330/1‑128 (MQ=255)
                                                                agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         >  1:422403/1‑128 (MQ=255)
                                                                agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         >  1:2646499/1‑128 (MQ=255)
                                                                agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         >  2:1242360/1‑128 (MQ=255)
                                                                 gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAAACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                        >  1:2180858/1‑128 (MQ=255)
                                                                    tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  2:2525538/128‑1 (MQ=255)
                                                                    tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  1:2004486/128‑1 (MQ=255)
                                                                    aaGCAGAAGATCTTAACAACTAAAGTAAACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  1:3779276/127‑1 (MQ=255)
                                                                     aGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatt                                                    <  1:93773/128‑1 (MQ=255)
                                                                       cAGAAGCTCTTAACAGCTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattat                                                  >  2:4212884/1‑128 (MQ=255)
                                                                       cAGAAGCTCTTAACAACTAAACTACACTCGATAATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattat                                                  <  2:4320112/128‑1 (MQ=255)
                                                                         gAAGCTCTTAACAACTAAACTTCACTCGATTATATAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGTGTTTAAAAATTATTCAAAAATTTGATTAttt                                                <  2:3222711/128‑1 (MQ=255)
                                                                           aGCTATTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTa                                              <  1:239714/128‑1 (MQ=255)
                                                                                ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGACTTTAAAAATTATTCAAAAATTTGATTATATTAAATCa                                         >  2:3523706/1‑128 (MQ=255)
                                                                                   acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACCCATTATTGTGAGTTTAAAAATTATTAAAAAATTTGATTATTTTAAATCAAtt                                      >  2:2895331/1‑128 (MQ=255)
                                                                                   acaacTAAACTACACTCGATTATTTAATTATATTTTATTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                      <  2:1175069/128‑1 (MQ=255)
                                                                                    caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAttt                                     >  1:807438/1‑127 (MQ=255)
                                                                                     aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    >  2:3995543/1‑128 (MQ=255)
                                                                                     aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    >  1:4311781/1‑128 (MQ=255)
                                                                                      aataaactaAAATAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   <  1:3130487/126‑1 (MQ=255)
                                                                                       ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                          >  2:638689/1‑88 (MQ=255)
                                                                                       ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAg                                  <  1:1625054/128‑1 (MQ=255)
                                                                                         aaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCt                                <  1:3697637/128‑1 (MQ=255)
                                                                                          aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                               <  2:907848/128‑2 (MQ=255)
                                                                                          aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCtg                               <  2:1846508/128‑2 (MQ=255)
                                                                                          aactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTc                               <  1:4007264/128‑1 (MQ=255)
                                                                                            ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCa                             >  1:4167856/1‑128 (MQ=255)
                                                                                              acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGACGCTCCAGc                           >  1:2282185/1‑128 (MQ=255)
                                                                                              acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           <  2:586091/128‑1 (MQ=255)
                                                                                              acacTCGATTATTTAATAATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           <  1:3183218/128‑1 (MQ=255)
                                                                                               cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTGGAGCt                          <  1:4374078/128‑1 (MQ=255)
                                                                                                acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                         >  2:2741985/1‑128 (MQ=255)
                                                                                                acTCCATTATTTAATTATATATTTATTTAATAAAAAAATCAATTAAACACACAAAAACTAACCCATTATGCTGAGTTTAAAAATTATTAAAAAATATTAAAATTTTAAATCAATTTaacaaaaaactt                         >  1:1679308/1‑118 (MQ=255)
                                                                                                 cTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                        <  2:4150410/128‑1 (MQ=255)
                                                                                                 cTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                        >  2:3368739/1‑128 (MQ=255)
                                                                                                    gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                     <  2:4487776/128‑1 (MQ=255)
                                                                                                    gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                     >  1:2072584/1‑128 (MQ=255)
                                                                                                     attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                    <  2:3161655/128‑1 (MQ=255)
                                                                                                     attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTAAAGCTACAGCTTCAACt                    >  2:4411764/1‑128 (MQ=255)
                                                                                                     aatattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAACTTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                    <  2:2617272/126‑1 (MQ=255)
                                                                                                        attTAATTATATTATTTTTTAAAATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTTAGTTTAAAAATAATTAAAAAATTTGATTATTTTAAATCAATTGAAGCTACACCTTCAACTAAt                 >  2:4570611/1‑128 (MQ=255)
                                                                                                          ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt               <  2:3714053/128‑1 (MQ=255)
                                                                                                          ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt               <  2:1686119/128‑1 (MQ=255)
                                                                                                           tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa              <  2:344890/128‑1 (MQ=255)
                                                                                                           tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa              <  2:835948/128‑1 (MQ=255)
                                                                                                             aTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGGTTATTTTAAATCAATTGAAGCTCCACCTTCAACTAAATAAGc            >  1:3064770/1‑128 (MQ=255)
                                                                                                                atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  2:1361620/1‑128 (MQ=255)
                                                                                                                atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  2:4072544/1‑128 (MQ=255)
                                                                                                                atatTTTTTATTAATATAAAAAGCAATTACACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATATTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         <  2:1304676/128‑1 (MQ=255)
                                                                                                                 tatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTc        <  2:3918602/128‑1 (MQ=255)
                                                                                                                  atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAGTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTTTTTAAATAAAATTAAGAGTCATCGTCAAATAATTTTGAtttatt      >  1:647826/1‑125 (MQ=255)
                                                                                                                  atTTTTTTTTAATATAAAAAGCAAATAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTAAAAAATTTGATTATTTTAACTCAATTGAAGCTCCAGCTTCAACTAATTTAGCATTCa       >  2:2433993/1‑128 (MQ=255)
                                                                                                                   tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt      <  1:19427/128‑1 (MQ=255)
                                                                                                                      ttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttc   <  1:178541/128‑1 (MQ=255)
                                                                                                                       tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct  >  1:508801/1‑128 (MQ=255)
                                                                                                                       tttttaATATAAAAAGCAATTAAACACACAAAAACACACCCATTATAGTGAGTTTAAAAATTATACAAAAATTAGATAATATTAAATCAATAGCAGCTCCAGCTTCAACTAAATTAGCTTTCATatct  >  2:1951541/1‑128 (MQ=255)
                                                                                                                              |                                                                                                                         
TATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484667‑484913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: