Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,801 A→G intergenic (‑110/+61) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,8010AG96.9% 7251.4 / NA 2081intergenic (‑110/+61)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base A (4/0);  major base G (919/1079);  minor base T (44/21);  total (981/1100)
Fisher's exact test for biased strand distribution p-value = 5.92e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2279 total aligned reads displayed.

AAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484675‑484925
                                                                                                                               |                                                                                                                             
    aGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAtt                                                                                                                          <  1:754449/128‑1 (MQ=255)
      tCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                                        <  2:448561/128‑1 (MQ=255)
          aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAaca                                                                                                                     >  2:1334719/1‑128 (MQ=255)
              cATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACaa                                                                                                                <  2:339449/128‑1 (MQ=255)
                  gATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAAc                                                                                                            <  1:834257/128‑1 (MQ=255)
                    tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                          >  1:2369722/1‑128 (MQ=255)
                    tttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                          <  2:1815637/128‑1 (MQ=255)
                    tttAATACTTTTATAGTTGACCAAAATATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                          <  2:1915311/128‑1 (MQ=255)
                    ttaaaTACTTTTATATTTGAACAAAATATTAGATAGATTATAGCAGAAGCTCTTAACAACTAAACTACACTCTATTATTTAATTTTATTTTTTTTTATTATAAAAAGCAATTAAACACACAAAAACTc                                                                                                          <  2:1216135/125‑1 (MQ=255)
                    tatAATCAATTTATAGTAGAACAATAATGACGATAGAGCAGATCAGAAGCTCTTAACAACTTAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                                          <  2:2894615/126‑1 (MQ=255)
                      tAATCCTTTTCTAGTTGTCCAAAAATTAAGATTGAGCATAGCCGTAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                                        <  1:1861618/128‑1 (MQ=255)
                       aaTCCTTTTATAGTTGACCAAAAAATAAGATATAGCATAGGAGAATCTATTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcc                                                                                                       <  1:2639396/128‑1 (MQ=255)
                        aTCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAcca                                                                                                      <  2:3475432/128‑2 (MQ=255)
                             tataaaGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataatg                                                                                                 <  1:3610675/123‑7 (MQ=255)
                              atataCTAGACCAAAAAATAAGAAAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCAATTATg                                                                                               <  2:1404827/127‑1 (MQ=255)
                               tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                              >  2:147221/1‑128 (MQ=255)
                                ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                             <  1:392483/128‑1 (MQ=255)
                                 taGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAtt                                                                                                   >  1:1597823/1‑122 (MQ=255)
                                   gTTGACCAAAAAATAATATAGATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGa                                                                                           <  1:422123/128‑1 (MQ=255)
                                     tGACCAAAAAATAAGATAGCCCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGtt                                                                                        <  2:253032/128‑1 (MQ=255)
                                       aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAAATACACTCGATTATTTAAATATATTATTTTTTAATAAAAAAAGCAATTAAACACACAAAAACTCAACCAAAATAGTGAGATTa                                                                                      >  2:739998/1‑128 (MQ=255)
                                        ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                                     <  2:2088587/128‑1 (MQ=255)
                                          aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaa                                                                                   <  1:150229/128‑1 (MQ=255)
                                          aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGATTaaaa                                                                                   >  2:3072689/1‑128 (MQ=255)
                                          aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATGAAACACACAAAAACACAGCCATAATGGAGAGTTGaaat                                                                                   >  1:1296781/1‑127 (MQ=255)
                                          aaaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACACGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACACATTATGGTTAATTTaaaa                                                                                   >  1:2911656/1‑128 (MQ=255)
                                           aaataTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  1:516902/128‑1 (MQ=255)
                                           aaaaaTAAGATATAGCATATACGAATCTCTTAACAACTAAACTACCCTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  2:1546963/128‑1 (MQ=255)
                                           aaaaaTAAGATAGATCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  1:3785511/128‑1 (MQ=255)
                                           aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                                  <  2:3819660/128‑1 (MQ=255)
                                             aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                                <  2:4388096/128‑1 (MQ=255)
                                             aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                                <  1:3706687/128‑1 (MQ=255)
                                               aTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCATAATGGGTGAGtttttgtgtgtt                                                                              <  1:1073090/128‑10 (MQ=37)
                                                tAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAattatt                                                                             >  2:3649444/1‑128 (MQ=255)
                                                 aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                            >  1:273376/1‑128 (MQ=255)
                                                  aGATAGAGCATAGCAGAATCTCATAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                                           <  1:4472712/128‑1 (MQ=255)
                                                       gagCATAGCAGAAGCTCTTAACAACTATACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                                      <  1:1757031/128‑1 (MQ=255)
                                                       gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCAt                                                                                                    >  1:999038/1‑99 (MQ=255)
                                                       gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                                      >  1:4561014/1‑128 (MQ=255)
                                                       gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTCAAAAATTATTCAAAAAt                                                                      >  1:679041/1‑128 (MQ=255)
                                                       gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                                     >  2:2806374/1‑128 (MQ=255)
                                                        agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCACCAATTATGGTGAGTTTAAAAATTAATCATAACtt                                                                     >  1:1001330/1‑128 (MQ=255)
                                                        agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAACCACAAAAACTCACCCATTATGGTGAGTTTATAAATTATTCAAAAAtt                                                                     >  1:3267301/1‑128 (MQ=255)
                                                        agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATAAAAAACGCAATTAAACACAAAAAAACTACCGAATTATGTTTAATTTAAAAATATATCAAAAAtt                                                                     >  1:2983187/1‑128 (MQ=255)
                                                        agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTAAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATAATTCAAAAAtt                                                                     >  2:4065309/1‑128 (MQ=255)
                                                         gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAAAACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                                    >  1:2180858/1‑128 (MQ=255)
                                                            tAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                                 <  1:2004486/128‑1 (MQ=255)
                                                              gCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGatta                                                               >  1:255825/1‑128 (MQ=255)
                                                               cAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattat                                                              <  1:4374420/128‑1 (MQ=255)
                                                                aGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGattatt                                                             <  2:3925235/128‑1 (MQ=255)
                                                                 gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTAACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGAATAttt                                                            >  1:60894/1‑128 (MQ=255)
                                                                    gCTCTTTACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                                         <  2:114658/128‑1 (MQ=255)
                                                                     ctctTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                                        <  2:431601/128‑1 (MQ=255)
                                                                        ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                                     <  1:3445107/128‑1 (MQ=255)
                                                                        ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                                     >  2:522440/1‑128 (MQ=255)
                                                                        ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                                     >  2:900748/1‑128 (MQ=255)
                                                                          aacaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                                   >  2:3722081/1‑128 (MQ=255)
                                                                            caacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTg                                                 >  2:1287953/1‑128 (MQ=255)
                                                                             aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                                >  2:3995543/1‑128 (MQ=255)
                                                                             aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                                >  1:4311781/1‑128 (MQ=255)
                                                                              actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTGTGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                               <  2:2297760/128‑1 (MQ=255)
                                                                                     taCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAg                                        >  2:19837/1‑128 (MQ=255)
                                                                                      acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGACGCTCCAGc                                       >  1:2282185/1‑128 (MQ=255)
                                                                                      acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                       >  1:3533097/1‑128 (MQ=255)
                                                                                      acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                                       <  2:1645759/128‑1 (MQ=255)
                                                                                       cacTCGATTATTAAATTATATTATTTTTTAATATAAAAAGCAAATAAACACACAATAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                                      <  2:323694/128‑1 (MQ=255)
                                                                                       cacTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTCAACACACAATAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                                      <  2:2369847/128‑1 (MQ=255)
                                                                                        acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                                     >  2:82447/1‑128 (MQ=255)
                                                                                        acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                                     >  2:2741985/1‑128 (MQ=255)
                                                                                        acTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                                    >  1:1354735/1‑128 (MQ=255)
                                                                                         cTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTc                                    <  2:3311924/128‑1 (MQ=255)
                                                                                          tagATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                                   <  1:4008294/126‑1 (MQ=255)
                                                                                           cGATTATTTAATTATATTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCa                                   >  1:1670217/1‑128 (MQ=255)
                                                                                           cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                                  >  1:1242189/1‑128 (MQ=255)
                                                                                           cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                                  >  2:2630935/1‑128 (MQ=255)
                                                                                            gaatattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                                 <  2:3547683/125‑1 (MQ=255)
                                                                                            gATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAAc                                 >  1:2072584/1‑128 (MQ=255)
                                                                                             attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                                >  2:3173091/1‑128 (MQ=255)
                                                                                             attattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACt                                <  2:588701/128‑1 (MQ=255)
                                                                                              ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTa                               >  1:3200998/1‑128 (MQ=255)
                                                                                              ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGATCCAGCTTCAACTa                               >  1:1450529/1‑128 (MQ=255)
                                                                                               tattTAATTATATTTTTTTTTAATAAAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTaa                              <  1:3135345/128‑1 (MQ=255)
                                                                                                attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                             <  1:1165024/128‑1 (MQ=255)
                                                                                                  ttAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAttt                           <  1:4165046/128‑1 (MQ=255)
                                                                                                     aTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGc                        <  2:4206844/128‑1 (MQ=255)
                                                                                                        atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCATCTTCAACTAATTTAGCttt                     >  2:2436396/1‑128 (MQ=255)
                                                                                                        atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt                     >  1:913447/1‑128 (MQ=255)
                                                                                                        atatTTTTATTTAATATAAAAAGCAATAAAACACACAAAAACTCACCCATAATGGTGAGTTTAAAAATAATTCAAAAAATTGATTATTTTAAAACAATTTAAGCACCAGCTTCAACTATTATAGCtat                     >  1:2843496/1‑126 (MQ=255)
                                                                                                          atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAATCTCCAGCTACAACTAATTTAGCTATCa                   >  2:641820/1‑128 (MQ=255)
                                                                                                           tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt                  <  1:19427/128‑1 (MQ=255)
                                                                                                           tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt                  <  2:4141701/128‑1 (MQ=255)
                                                                                                            ttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt                 >  1:3366144/1‑128 (MQ=255)
                                                                                                            atttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAtt                 <  1:2454700/127‑1 (MQ=255)
                                                                                                               tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct              <  2:4565310/128‑1 (MQ=255)
                                                                                                               tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct              >  1:409961/1‑128 (MQ=255)
                                                                                                                   ttaatataaaaagCAATTAAACACACAAAAACTCACACATTATGTTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCa           <  1:3072689/121‑1 (MQ=255)
                                                                                                                        ataaaaagCAAGTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGcttc      <  2:1079687/126‑1 (MQ=255)
                                                                                                                          aaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGcttctt    >  2:2976617/1‑128 (MQ=255)
                                                                                                                          aaaaagCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGcttctt    >  2:1728177/1‑128 (MQ=255)
                                                                                                                          aaaaagCAATTAAAAACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGTAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGcttctt    <  2:2178312/128‑1 (MQ=255)
                                                                                                                               |                                                                                                                             
AAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCTTCAGCTTCTTCT  >  CP016816/484675‑484925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: