Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 516,362 T→C intergenic (+193/+313) JCVSYN2_02375 → / ← JCVSYN2_02380 antitermination protein NusG/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816516,3620TC99.0% 8463.4 / NA 2286intergenic (+193/+313)JCVSYN2_02375/JCVSYN2_02380antitermination protein NusG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/4);  major base C (1020/1229);  minor base A (9/13);  total (1030/1256)
Fisher's exact test for biased strand distribution p-value = 8.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2310 total aligned reads displayed.

AAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTTAATAA  >  CP016816/516237‑516486
                                                                                                                              |                                                                                                                            
aaaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAacat                                                                                                                            <  1:1665767/128‑1 (MQ=255)
 aaCATAAAATATATTTTTTAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAtt                                                                                                                           <  2:1765378/128‑1 (MQ=255)
 aaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAtt                                                                                                                           <  2:1111136/128‑1 (MQ=255)
  aCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAATGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTACTTTTAATTAAAATTTTAATAAAAAAACAttt                                                                                                                          <  2:810473/128‑1 (MQ=255)
  aCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACAttt                                                                                                                          <  2:667260/128‑1 (MQ=255)
    aTAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTa                                                                                                                        <  2:3663210/128‑1 (MQ=255)
      aaaaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGTTATTTTTTAAAGATTATTTAAAATTTTTTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGt                                                                                                                      >  2:2653238/1‑128 (MQ=255)
      aaaaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAATCATTTTTGt                                                                                                                      >  1:2435077/1‑128 (MQ=255)
      aaaaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAAATTTGTTTTTATTTTTAATTAAATTATTAATAAAAAAACATTTTAGt                                                                                                                      >  2:2419280/1‑128 (MQ=255)
          tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                                  <  1:2271330/128‑1 (MQ=255)
           atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTAttt                                                                                                                  <  2:926927/128‑1 (MQ=255)
           atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                                 >  1:1750883/1‑128 (MQ=255)
           atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                                 >  2:3767373/1‑128 (MQ=255)
           atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTg                                                                                                                 <  1:2492298/128‑1 (MQ=255)
            tatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGc                                                                                                                >  1:2583281/1‑128 (MQ=255)
             atTTTTAAAAAATATGTTTTTATAAGATGAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTATTTAATATTTTAATAAAAGAACATTTTAGTATTTGCa                                                                                                               >  1:1406383/1‑128 (MQ=255)
              tttttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCaa                                                                                                              <  2:2557544/128‑1 (MQ=255)
                   aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATtata                                                                                                         >  2:4165167/1‑128 (MQ=255)
                    aaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTTTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaa                                                                                                        >  1:1160619/1‑128 (MQ=255)
                    aaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTATTATTTTCAATTATaa                                                                                                        >  1:4384935/1‑128 (MQ=255)
                          atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                  <  2:1588864/128‑1 (MQ=255)
                            gTTTTTATATGATTAGTATAATAGAGTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTATTTTTTAATTTAAATTTTAATATAAAATCTTTTTAGTATTTGCAATTATAAAAAAGttt                                                                                                <  1:3051312/128‑1 (MQ=255)
                            gTTTTTATAAGATTAGAAATAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTTAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGttt                                                                                                <  2:218846/128‑1 (MQ=255)
                              ttttATAAGAATAGAAAAAAAGCTTATATTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTAt                                                                                              <  1:4166559/128‑1 (MQ=255)
                               tttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATg                                                                                             >  1:3392931/1‑128 (MQ=255)
                               tttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATg                                                                                             >  1:358793/1‑128 (MQ=255)
                                ttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGa                                                                                            <  1:2676942/128‑1 (MQ=255)
                                ttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGa                                                                                            <  1:671930/128‑1 (MQ=255)
                                 tataAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGat                                                                                           <  2:2745912/128‑1 (MQ=255)
                                  ataAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATaaaa                                                                                                      <  1:2753137/116‑1 (MQ=255)
                                  ataAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGata                                                                                          >  2:3842805/1‑128 (MQ=255)
                                  ataAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGata                                                                                          <  1:1580149/128‑1 (MQ=255)
                                    aaGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataat                                                                                        >  1:1094452/1‑128 (MQ=255)
                                     aGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                                       <  2:3076248/128‑1 (MQ=255)
                                     aGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                                       <  1:749280/128‑1 (MQ=255)
                                        ttAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                                    <  1:1921516/128‑1 (MQ=255)
                                          aGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATAAAAATTTTAATAAAAAAACATTTTAGTATTTTCAATTATAAAAAAGTTTATGATAATATAcat                                                                                  >  1:1819308/1‑126 (MQ=255)
                                           gAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTa                                                                                 >  1:3543226/1‑128 (MQ=255)
                                           gAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTa                                                                                 >  1:880303/1‑128 (MQ=255)
                                           gAAAAAAAGCGTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTa                                                                                 >  1:3137987/1‑128 (MQ=255)
                                             aaaaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTAt                                                                                <  2:2819947/128‑1 (MQ=255)
                                             aaaaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATAATAATATACatat                                                                                >  2:3957851/1‑128 (MQ=255)
                                                aaatGCTTATATTAAGGGCTTTTTTAAATATTATTTAAAATTTTCTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                             <  1:1254045/128‑1 (MQ=255)
                                                aaaaGCTTATTTTAATGTCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAATATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                             <  2:3009069/128‑1 (MQ=255)
                                                aaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                             <  2:1480708/128‑1 (MQ=255)
                                                aaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                             <  2:497568/128‑1 (MQ=255)
                                                aaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                             >  2:2362161/1‑128 (MQ=255)
                                                 aaaGCTTATTTTAAGGTCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGata                                                                                          <  1:3453401/114‑1 (MQ=255)
                                                    gCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAata                                                                           <  2:3518847/126‑1 (MQ=255)
                                                    gCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAAtata                                                                         <  2:587720/128‑1 (MQ=255)
                                                     cTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAg                                                                        <  1:580815/128‑1 (MQ=255)
                                                     cTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAg                                                                        >  1:2090451/1‑128 (MQ=255)
                                                         ttttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATa                                                                    <  1:4451298/128‑1 (MQ=255)
                                                         ttttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATa                                                                    <  1:4082051/128‑1 (MQ=255)
                                                          tttAATTGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                                   <  2:1980030/128‑1 (MQ=255)
                                                             aaGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAAt                                                                     >  1:3313611/1‑123 (MQ=255)
                                                                ggCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACt                                                             <  2:3337678/128‑1 (MQ=255)
                                                                  cTTTTTTATAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAg                                                           <  2:1856125/128‑1 (MQ=255)
                                                                  cTTATTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAg                                                           <  1:944305/128‑1 (MQ=255)
                                                                    tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                         <  2:4073967/128‑1 (MQ=255)
                                                                    tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                         >  2:3418556/1‑128 (MQ=255)
                                                                    tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                         >  2:3588209/1‑128 (MQ=255)
                                                                      tttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaaa                                                       >  2:4475766/1‑128 (MQ=255)
                                                                       ttAAATATTATTTAAAATTTTGTTATTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAag                                                      >  1:482159/1‑128 (MQ=255)
                                                                        tAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAaga                                                     <  1:3227234/128‑1 (MQ=255)
                                                                        tAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAaga                                                     >  1:3650863/1‑128 (MQ=255)
                                                                         aaaGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAagag                                                    <  2:2903915/128‑1 (MQ=255)
                                                                             attattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAat                                                <  2:867987/128‑1 (MQ=255)
                                                                              ttattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCGATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAata                                               <  1:2966792/128‑1 (MQ=255)
                                                                              ttattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAata                                               <  1:2856674/128‑1 (MQ=255)
                                                                              ttattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAata                                               <  2:1933655/128‑1 (MQ=255)
                                                                               tattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAatat                                              >  2:1028248/1‑128 (MQ=255)
                                                                                attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTATAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             >  2:2266506/1‑128 (MQ=255)
                                                                                 tttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatta                                            <  1:3908657/128‑1 (MQ=255)
                                                                                 tttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatta                                            >  1:372965/1‑128 (MQ=255)
                                                                                  ttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtattat                                           <  1:1993290/128‑1 (MQ=255)
                                                                                   tAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                          <  1:3985733/128‑1 (MQ=255)
                                                                                   tAAAATTTTGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                          <  2:1340016/128‑1 (MQ=255)
                                                                                          ttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGa                                   <  1:3826243/128‑1 (MQ=255)
                                                                                          ttGTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATAATATTAGTATGa                                   >  1:237291/1‑128 (MQ=255)
                                                                                            gTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATa                                 >  1:4293299/1‑128 (MQ=255)
                                                                                            gTTTTTATTTTTAATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATa                                 <  2:4392230/128‑1 (MQ=255)
                                                                                                ttatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATAAGTATGATAGCGa                             >  1:1000475/1‑128 (MQ=255)
                                                                                                 tatttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaa                            >  2:118373/1‑128 (MQ=255)
                                                                                                  atttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaat                           >  1:1322967/1‑128 (MQ=255)
                                                                                                    ttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaatta                         <  2:3155701/128‑1 (MQ=255)
                                                                                                    ttttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaatta                         >  1:1477246/1‑128 (MQ=255)
                                                                                                     tttaATTAAAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaattaa                        <  2:2099923/128‑1 (MQ=255)
                                                                                                         attaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAtttt                    <  1:815186/128‑1 (MQ=255)
                                                                                                         attaaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAtttt                    >  1:4539692/1‑128 (MQ=255)
                                                                                                           taaAATTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTa                  <  2:3901482/128‑1 (MQ=255)
                                                                                                              aaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaaa               >  2:1265997/1‑128 (MQ=255)
                                                                                                              aaTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaaa               <  2:4538869/128‑1 (MQ=255)
                                                                                                               aTTTTAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAat              >  2:140217/1‑128 (MQ=255)
                                                                                                                  ttAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAattta           >  2:4294356/1‑128 (MQ=255)
                                                                                                                  ttAATAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAattaa           >  1:2190665/1‑126 (MQ=255)
                                                                                                                   taataaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATTATAATATACCTATTAATATAGAATAAACAACTAGAAAATAGAAATATTATTAGAATGATAGCGAATTAATTTTTAAAatttat          >  2:3255088/7‑128 (MQ=255)
                                                                                                                   taataaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAataaat          <  2:1662354/122‑5 (MQ=255)
                                                                                                                         aaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaat    >  2:2489028/1‑128 (MQ=255)
                                                                                                                         aaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATAATTAGTATGAAAGCTAATTAATTTTTAAAATTAATTTtaat    >  2:761726/1‑128 (MQ=255)
                                                                                                                              |                                                                                                                            
AAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAAAAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTTAATAA  >  CP016816/516237‑516486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: