Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT97.5% 4213.7 / 1.0 1467intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (9/28);  new base T (958/471);  total (968/499)
Fisher's exact test for biased strand distribution p-value = 3.53e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1529 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346
                                                                                                                                |                                                                                                                                
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                <  2:379153/128‑1 (MQ=255)
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                <  1:1654428/128‑1 (MQ=255)
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGAAAATTTTtaa                                                                                                                                >  2:1824516/1‑128 (MQ=255)
  tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaa                                                                                                                              >  2:4244805/1‑127 (MQ=255)
  tACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaa                                                                                                                              >  2:1586709/1‑127 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                           <  2:912481/128‑1 (MQ=255)
     tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTATGTATATTCAAATTTATAAAAaat                                                                                                                           >  2:3004932/1‑128 (MQ=255)
         aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                       >  1:4476617/1‑128 (MQ=255)
          ttGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaatt                                                                                                                      <  1:4560770/128‑1 (MQ=255)
            gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                    >  1:2440222/1‑128 (MQ=255)
              aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  <  1:2626303/128‑1 (MQ=255)
              aTTAATTTACTAGTTGATAATATTTAGTTTAATTAATATATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                  <  2:2183461/128‑1 (MQ=255)
                 actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                               >  2:1455628/1‑128 (MQ=255)
                 actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAAtt                                                                                                               >  1:1813703/1‑128 (MQ=255)
                  ctttactAGCTGATAATTTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                              <  1:3125354/128‑1 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACtttt                                                                                                             >  1:4042895/1‑126 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                            >  2:4083197/1‑128 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                            <  2:3721623/128‑1 (MQ=255)
                      actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTaaa                                                                                                          >  1:4403185/1‑128 (MQ=255)
                      actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATAAATTTTAACTTGTaaa                                                                                                          >  2:1726297/1‑128 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCTCTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCATATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                        >  2:3533246/1‑128 (MQ=255)
                        tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                        >  1:2087924/1‑128 (MQ=255)
                           ttgaaaaaaataaGTTTCATTAATTTATCGCACTTGTATGAATTATTAATAAACTAAAAAAATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAAATAATTTGAACTTGTAAAAAATa                                                                                                     <  1:486613/116‑1 (MQ=255)
                            tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                    >  1:720495/1‑128 (MQ=255)
                              ataataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCt                                                                                                  >  2:187838/1‑128 (MQ=255)
                              ataataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCt                                                                                                  >  2:1377278/1‑128 (MQ=255)
                               taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtt                                                                                                 >  2:4563466/1‑128 (MQ=255)
                               taataTTTAGTTTCATTAATTTATCGCACTTGTATGAACTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGTAAAAAATAGCtt                                                                                                 >  2:3446142/1‑128 (MQ=255)
                                aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                >  2:677360/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttat                                                                                               >  2:3586969/1‑126 (MQ=255)
                                 aaatTTAGGTTTAATTATTTCTAGCACTTTTATTAATTAGTAAGAAACTAAAAATATTATCTTTTTAGATATTTTTTGTTTATTCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGCtttt                                                                                               <  1:2258036/126‑1 (MQ=255)
                                  aattttcttttATTAATTTAACGCCCTTGTTTTTATTTATTACAAAATAAAAAAAATATCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAAATAATTTGAACATTTAAAAAAAACCttttt                                                                                              <  1:1109210/121‑1 (MQ=255)
                                      ttatttCATTAATTTATAGCACTTTTATTTATTATTAATTAAATAAAAAATATTTTTTTTTTTATTTTTTTTGTTTTTTCAAATTTTTAAAAAATTAATTTTTACTTGTAAAAAATAGCttttttttt                                                                                          <  1:2942423/125‑1 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttt                                                                                          >  2:793363/1‑128 (MQ=255)
                                      tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                          >  2:240396/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                        >  2:3165135/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                        >  1:127218/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                        >  1:3568847/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTAAGAAATTTTTTGTATATGAAAATTTATAAAAAATTAATTTGAACTTGTAAAAAATAGCtttatattttt                                                                                        >  1:2865035/1‑128 (MQ=255)
                                           tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAtttttttttttttt                                                                                     >  2:1285369/1‑128 (MQ=255)
                                           tCATTAATTTATAGCAATTGTATGAATTATTAATAAAATAAAAATAATATTTTTTTATTTATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAAAAGCtttttttttttttt                                                                                     <  2:1211850/128‑1 (MQ=255)
                                            cATTAATTTATCGCACTTGTATTAATATGTAAGAAACTAAAACTTTTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAGCctttttttttttttt                                                                                    <  2:4141684/128‑1 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                    >  1:3656740/1‑128 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                    >  1:528208/1‑128 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAttttttttttttttt                                                                                    >  1:58197/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                   >  1:3777269/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTAGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAATATAGCtttttttttttttttt                                                                                   >  1:1737922/1‑128 (MQ=255)
                                                aaTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTATAAAATAGAttatattttttttttattt                                                                                >  1:3836983/1‑128 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttttt                                                                              >  2:3934070/1‑126 (MQ=255)
                                                      actgaaatttATTAATTAGTAAAAAAATAAAACTAATATTATTTTTTATATTTTTTTTATTATCAAAATTTTAAAAAATTAAATTTAAATTGTAAAAAAATGCTTTTTTTTTTTTTTTTTTATAATgg                                                                         <  1:1369456/119‑1 (MQ=255)
                                                       agcagtttttttaaTTAGTAAGAAAATAAAAAATATATCTTTTTATATATTTTTTGTATAATTAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAAATTCCTTTTTTTTTTTTTTTTTTATAATgg                                                                         <  2:4422555/116‑1 (MQ=255)
                                                        gCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTaa                                                                       >  2:1101708/1‑128 (MQ=255)
                                                         cACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtatttttaat                                                                       >  1:2178674/1‑121 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                >  2:567538/1‑68 (MQ=255)
                                                               tattcaatAGTAACAACATAATAATATTTTCTTTTTTTATTTTTTTTTTATATTCAATTTTTTAAAAAATAAATTTTAACTTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                              <  2:2907785/121‑1 (MQ=255)
                                                                aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATTTTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTATTTTTTTTTTTTTTATAATTGAAAATTAAAt                                                               >  1:939553/1‑128 (MQ=255)
                                                                 tGAAATAGTTATAATATAAAAATATTATTTTTTTTTAAATTTTTTTTATATTGAAATTTTTAAAAAATTAATTTTCACTTTTAAAAAATAGGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                              <  2:3286110/128‑1 (MQ=255)
                                                                   tatatttttataaaataaaaataTTTTATTTTTTTTTATTTTTTTTATTTTTAAATTTTTTAAAAATTTATTTTAAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                              <  2:4310539/117‑1 (MQ=255)
                                                                    aTTAGTAATAAAATAAAACTTTTATTTTTTTAGATATTTTTTGTTTATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                          <  2:2461234/128‑1 (MQ=255)
                                                                    aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTa                                                           >  2:4492000/1‑128 (MQ=255)
                                                                    aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATTAAAATTTTat                                                          >  2:3378179/1‑127 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTATTAAAAAATTAATTTGAACTAGTAAAAAATACATTATTTTTTTTTTTATATTATAATTTAAAAATAAAatttta                                                          >  1:2370981/1‑126 (MQ=255)
                                                                       tttttataaattaaaaataTTATTTTTTTTTTTTTTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAAT‑‑‑TGATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                       <  1:4196635/121‑1 (MQ=255)
                                                                       tatttattaaataaaaataTTATTTTTTTATTTATTTTTTTTTTATTAAAATTTTTAAAAAATTTATTTTTAATTTTTAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                        <  1:3255165/117‑1 (MQ=255)
                                                                             gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTAAGTtttttt                                                   >  1:3409858/1‑128 (MQ=255)
                                                                             gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTAAAATATAATTTTTTGTtttttt                                                   >  1:3459218/1‑128 (MQ=255)
                                                                                actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATTTAAATATAAATTTTATTTATTttgt                                                 >  2:102597/1‑128 (MQ=255)
                                                                                 atataaatattttatttTTTTATATTTTTTTTTTTTTGAAATTTTTAAAAAATTAATTTGTAATTGTAAAAAAAATATTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                <  1:4133349/121‑1 (MQ=255)
                                                                                 ataaaaataTTTTTTTTTTATTTATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgtt                                                <  2:16525/127‑1 (MQ=255)
                                                                                  taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATTTAAATATAAATTTTTATTATTtttttga                                              >  2:2012689/1‑127 (MQ=255)
                                                                                   aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAAAAATATAAATATAAATTTTAGTTATAttttttaa                                             >  1:2535687/1‑125 (MQ=255)
                                                                                   aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATATTTTAAATATAAATTTTAGTATtttttttttata                                          >  1:1502038/1‑122 (MQ=255)
                                                                                      tctaTTTTGTTTTTTTTTATTTTTTGTTTTTTTAATTTTTTAAAAAATTAATTTGTAATTTTTATAAAATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                          <  2:2754483/127‑1 (MQ=255)
                                                                                       atattttttttttttatttttttttttTTTGTTAATTTTTAAAAAATTATTTTTTAATTTTAAAAAATTTATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                         <  2:2841821/127‑1 (MQ=255)
                                                                                        tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTT‑‑‑TAATGTAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                      >  1:84563/1‑128 (MQ=255)
                                                                                        tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAAAATAATTTTTATGTTTTTTGTTTTATaatt                                        >  2:2514765/1‑126 (MQ=255)
                                                                                          atttatttttATTTATTTTTTTTATAATTAAATTTTTATAAAATTTAATTTTAAATTTAAAAAAATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                      <  1:1942226/123‑1 (MQ=255)
                                                                                             tttttttATATTTTTTTTTTATATTCTAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                    <  1:2029945/126‑1 (MQ=255)
                                                                                             agtttttATTTTTTTTTTTTATAATCAAATTTTTAAAAAATTTATTTTTTCTTTTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTa                                   <  1:4284791/126‑1 (MQ=255)
                                                                                                 tttttatatTTTTTTTATTATTAAATTTTTAAAAAATTAAATTTAAATTGTAAAAAATAG‑‑‑ATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                             <  2:2250149/123‑1 (MQ=255)
                                                                                                 tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑CTTTTTTTTTTTTTTTTATAATTGAAATTAAAATTTTATTAATTTTTTTTTATAATTATATaaaaaa                              >  2:855990/1‑128 (MQ=255)
                                                                                                  ttttattttttttttttATTTAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAAATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaa                              <  2:1948971/121‑1 (MQ=255)
                                                                                                  ttATATATTTTTTTTATATTAAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAA‑‑‑‑TTTCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                           <  2:2591333/128‑1 (MQ=255)
                                                                                                    tttatttttttttttATTAAATTTTTTAAAAATTTATTTTTAACTTTTAAAAATATTTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTGTATAATAATCTaaaaaata                            <  2:2322523/123‑3 (MQ=255)
                                                                                                    aGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATGAAAATAAAAATTTTATGTATTTTTTTGTATAATTATCTaaaaaaaa                            >  2:2677589/1‑127 (MQ=255)
                                                                                                      attttttttttATTTTTAAATTTTTAAAAAAATAATTTGAAATTTTAAAAAATA‑‑TATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                         <  2:501168/125‑1 (MQ=255)
                                                                                                       ttttttttttATTTTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                         <  1:1600838/126‑1 (MQ=255)
                                                                                                          tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTTAAATAAAAATTTAAGTTATTTTTTTTTATAATTATATAAAAAAATATaaat                      >  2:4012609/1‑127 (MQ=255)
                                                                                                              tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAATAAAATTTTTAGTTTTTTTTTTTTATATTTATAAAAAAAAAAAAAAAGTaaa                  >  1:1003325/1‑128 (MQ=255)
                                                                                                                ttttatgataaaTTTTAAAAAATTTTTTTGTATTTTTAAAAAATTTTGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                 <  2:365666/119‑1 (MQ=255)
                                                                                                                tataTGCAAAATTTTAAAAAATTAATTTTAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATATATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa                <  1:3943930/128‑1 (MQ=255)
                                                                                                                 ttttattaaaaTTTTTAAAAAAATAATTTTAAAATTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                  <  1:1161248/120‑1 (MQ=255)
                                                                                                                 atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAATTATAATTTTTAGTTATTTTTTTTTATAATTTTATAAAAAAATATAAAGTaaaaaa               >  2:3858451/1‑128 (MQ=255)
                                                                                                                   tttttaaaaTTTTTAAAAATTTTATTTTAATTTTTATAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat              <  2:1511691/122‑1 (MQ=255)
                                                                                                                       gCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTTTTGTATAATTAAATAAAAAAATAGAAATAAAAAAATAtt           >  1:3715950/1‑128 (MQ=255)
                                                                                                                        cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAATAtt           >  2:2120084/1‑128 (MQ=255)
                                                                                                                        cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTGTTTATTTTTTGTCTATTTATATAAAAAAAAATAATATAAAAAATAttt          >  1:3029777/1‑127 (MQ=255)
                                                                                                                         aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATGTAAATATAAATTTTAGGTATTTTTTTTTATTATTATATAAAAAAATATAAATGAAAAAATATtttt        >  2:3109804/1‑128 (MQ=255)
                                                                                                                         aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTCTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTg          <  1:1431135/128‑1 (MQ=255)
                                                                                                                         aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTTGTTATTTTGTTTTATAAGTATCTAAAAAAATATAAATTAAAAATTATTGt         >  1:2752421/1‑128 (MQ=255)
                                                                                                                                |                                                                                                                                
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165093‑165346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: