BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.5%	5177.6 / NA	1680	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (6/3); new base T (866/803); total (873/807)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	98.9%	5001.8 / NA	1680	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (3/15); new base T (870/792); total (873/807)
Fisher's exact test for biased strand distribution p-value = 3.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1762 total aligned reads displayed.

ATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATA  >  CP016816/420033‑420283

||

  taaattCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatt                                                                                                                            <  1:2704668/128‑1 (MQ=255)

     attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaatttta                                                                                                                         <  2:3395976/128‑4 (MQ=255)

     attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTAATATCACTTTTTTAAAAAATttttaattata                                                                                                                         >  1:1671818/1‑126 (MQ=255)

      ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                        <  1:2341427/128‑5 (MQ=255)

        cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                      <  2:1331335/128‑1 (MQ=255)

         attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                     <  2:1978363/128‑1 (MQ=255)

         attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                     >  2:1376638/1‑128 (MQ=255)

         attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                     >  2:1252786/1‑128 (MQ=255)

         attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                     >  1:3176816/1‑128 (MQ=255)

          ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                    <  2:3861763/128‑1 (MQ=255)

             atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                 >  2:1444043/1‑128 (MQ=255)

              tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                >  1:2584751/1‑128 (MQ=255)

               aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAACACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                               >  1:3983281/1‑128 (MQ=255)

               aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTCAat                                                                                                               >  1:1998326/1‑128 (MQ=255)

                cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                              <  2:256974/128‑1 (MQ=255)

                  tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                            <  1:1108914/128‑1 (MQ=255)

                   ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                           >  1:98426/1‑128 (MQ=255)

                   ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                           <  2:4338717/128‑1 (MQ=255)

                     aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                         <  2:2144213/128‑1 (MQ=255)

                       tctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTc                                                                                                       >  1:3064759/1‑128 (MQ=255)

                        ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                      <  2:1478777/128‑1 (MQ=255)

                         tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                     >  1:2513382/1‑128 (MQ=255)

                         taaTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATAATTATATTAGTTGATTTATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGATTTATTTTTCaa                                                                                                     <  2:593280/126‑1 (MQ=255)

                          cATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                    <  2:3947541/128‑1 (MQ=255)

                          cATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                    <  1:3699956/128‑1 (MQ=255)

                           aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   >  2:4092351/1‑128 (MQ=255)

                           aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCAATTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   >  1:4480866/1‑128 (MQ=255)

                           aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATAAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                   <  1:157539/128‑1 (MQ=255)

                           aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAAAACTTTTATTAGTTGATGTATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTAATTTTCAAAt                                                                                                   >  1:4060526/1‑128 (MQ=255)

                             aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                 >  2:3781698/1‑128 (MQ=255)

                               aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTATTCAAATTGtt                                                                                               >  1:2175785/1‑128 (MQ=255)

                                 cTAAGTTTCTAAAATTATTTTATCATTTAAAATAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaa                                                                                             <  2:459820/128‑1 (MQ=255)

                                   aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                           <  2:2502831/128‑1 (MQ=255)

                                   aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                           <  2:2717587/128‑1 (MQ=255)

                                    attttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                          <  2:3482858/126‑1 (MQ=255)

                                    aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                          <  1:361420/128‑1 (MQ=255)

                                       ttCTAAAATTATTATATCATTTAAAAAATTAATAGTTTTTTTAGTTGATATATTAGAAAACTACTAGGACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                       <  1:2919072/128‑1 (MQ=255)

                                        tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                      <  1:2058640/128‑1 (MQ=255)

                                        tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAttt                                                                                      <  1:880811/128‑1 (MQ=255)

                                         cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTAATATTAAAAATGTTAAAATAttta                                                                                     >  2:2976335/1‑127 (MQ=255)

                                         cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     <  1:439392/128‑1 (MQ=255)

                                         cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                     >  2:1620556/1‑128 (MQ=255)

                                          tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                    <  2:4262307/128‑1 (MQ=255)

                                            aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                  >  2:2285043/1‑128 (MQ=255)

                                                 attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                             >  2:3717353/1‑128 (MQ=255)

                                                 attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                             >  1:3861939/1‑128 (MQ=255)

                                                    ttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                          <  1:4208786/128‑1 (MQ=255)

                                                     tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                         <  1:3288839/128‑1 (MQ=255)

                                                     tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTG‑ATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATCt                                                                        >  1:479798/1‑128 (MQ=255)

                                                      aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                        <  1:1000320/128‑1 (MQ=255)

                                                       tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                       <  2:776731/128‑1 (MQ=255)

                                                       tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                       <  2:1801178/128‑1 (MQ=255)

                                                       tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                       <  1:775182/128‑1 (MQ=255)

                                                         aTTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAtt                                                                     >  2:4141421/1‑128 (MQ=255)

                                                           ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                   <  1:2281611/128‑1 (MQ=255)

                                                            tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                    <  2:2050310/110‑1 (MQ=255)

                                                               aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                               <  1:1375165/128‑1 (MQ=255)

                                                               aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCaa                                                               <  2:3627510/128‑1 (MQ=255)

                                                                 aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtt                                                             >  2:830375/1‑128 (MQ=255)

                                                                  aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAttt                                                            <  2:691675/128‑1 (MQ=255)

                                                                   ataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                           >  2:4501223/1‑128 (MQ=255)

                                                                     aataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtattat                                                         >  1:4552255/1‑128 (MQ=255)

                                                                      ataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATg                                                        >  2:3052914/1‑128 (MQ=255)

                                                                          ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTct                                                    >  2:3565765/1‑127 (MQ=255)

                                                                          ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCg                                                    >  2:3126631/1‑128 (MQ=255)

                                                                          ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCg                                                    >  1:3491157/1‑128 (MQ=255)

                                                                           tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGATCG‑t                                                  >  2:1185330/1‑128 (MQ=255)

                                                                             tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                          >  1:1842242/1‑87 (MQ=255)

                                                                                tatttGATAAATTATAAAACTACTATCACTTTTTTAAAAAAATTTTAATTTTAAAAAATTAATTATTTTTCAAATTGTTAAATTATTTTAAAAACATTATATATTAATAAATTTTATGCTCG‑tttttt                                             <  2:4017428/125‑1 (MQ=255)

                                                                                taGTTGAATAATTAGAAAACTACTATCAAGTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATCAATTAATCAATTTTATGCTCG‑tttttt                                             <  2:512429/128‑1 (MQ=255)

                                                                                   tttttatatTATTAAAATAATATGACTTTTTTAAAAAATTTTTAATTTTAATTAATTAATTTTTTTTAAAATTTTTTAATTATTTTTAAATCATTTTTTTTTAATAAATTTTTTTCTCG‑ttttttttt                                          <  1:4136878/124‑1 (MQ=255)

                                                                                   ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCt‑ttttttttt                                          >  2:1098224/1‑128 (MQ=255)

                                                                                   ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑ttttttttt                                          >  2:2645049/1‑128 (MQ=255)

                                                                                    tGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAGCTGAATTATTTTTGAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑ttttttgttt                                         >  1:2426780/1‑128 (MQ=255)

                                                                                        atatTATAAAAATAATATAAATTTTTTTAAAAATTTTTAATTTTAATAAATTAATTTTTTTTTAAATTTTTTAATTTTTTTTAATTAATTATATATTAATTAATTTTAtttttt‑tttttttttttttt                                     <  1:147317/128‑1 (MQ=255)

                                                                                        atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTCG‑tttttttttttttt                                     >  2:2208174/1‑128 (MQ=255)

                                                                                        atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑tttttttttttttt                                     >  2:2354840/1‑128 (MQ=255)

                                                                                        atatTAGAAAACTACTAGCACTTTTTTAAAAAATTATTAATTTTAATAACTGAATTATTTTACAAATTGTTAAATTATTTTCAAATAATTAAATATAAATAAATTTTATGCTCG‑tttttttttttttt                                     >  1:3438150/1‑128 (MQ=255)

                                                                                         tatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATTCTCt‑ttttttttttttttt                                    >  2:1714507/1‑128 (MQ=255)

                                                                                          atTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑tttttttttttttttt                                   >  2:1886352/1‑128 (MQ=255)

                                                                                           ttAGAAAACAAATAGAAATTTTTTAAAAATTTTTTAATTTTAAAAACTGAATTATTTTTCAAATTTTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑tttttttttttttttta                                  <  1:3928246/128‑2 (MQ=255)

                                                                                              taaaaCTAATATCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATCTATTAATCAATTTTATGCTCG‑TTTTTTTTTTTTTTTTTACa                               <  2:4435352/127‑1 (MQ=255)

                                                                                              gAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAAtttt                                                           <  1:638675/101‑1 (MQ=255)

                                                                                                aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑TTTTTTTTTTTTTTTTTAaaaa                             >  1:3562878/1‑128 (MQ=255)

                                                                                                  actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑TTTTTTTTTTTTTTTTTTaaaaaa                           >  1:538208/1‑128 (MQ=255)

                                                                                                  acaactaTCACTTTTTTAAAAATTTTTTAATTTTAATAACTTAATTATTTTTAAAATTTTTAAATTATTTTCAAATAATTATATATTAATAAATTTTATGCTCG‑TTTTTTTTTTTTTTTTTACaaaaa                           <  2:290073/125‑1 (MQ=255)

                                                                                                   ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATATATTAATCAATTTTATGATAG‑tttttttttttttttaaaaaaaata                          >  2:3218317/1‑118 (MQ=255)

                                                                                                     actaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATATTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑TTTTTTATTTTTTTTTTaaaataaaat                        >  2:1032467/1‑128 (MQ=255)

                                                                                                      ctaTCAATTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTCAAATTTTTAAATTATTTTAAAATAATTATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTACAAAAatat                        <  1:2816334/128‑1 (MQ=255)

                                                                                                      ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTACAAAAATATaa                      >  1:4275568/1‑128 (MQ=255)

                                                                                                       tatcACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTTTTAATCAATTTTATGCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATaa                      <  2:2431138/125‑1 (MQ=255)

                                                                                                        atcaattttttAAAAAATTTTTAATTTTAATAAATTTATTTTTTTTAAAATTTTTAAATTATTTTAAAAAAATTATATATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTACAAAAATATaa                      <  2:4193336/123‑1 (MQ=255)

                                                                                                        aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATAat                     >  1:3784873/1‑128 (MQ=255)

                                                                                                         gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑TTTTTTTTTTTTTTTTTTAAAAAAAATaaat                    >  1:1727243/1‑126 (MQ=255)

                                                                                                          cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑TTTTTTTTTTTTTTTTACAAAAATATAattta                  >  2:2802653/1‑128 (MQ=255)

                                                                                                              tttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑‑TTTTTTTTTTTTTTTACAAAAATATAatttatttaa             >  1:1304373/1‑126 (MQ=255)

                                                                                                                 ttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATATTATGCTCG‑TTTTTTTTTTTTTTTTTAAAAAAATATAAtttattttat            >  2:892783/1‑128 (MQ=255)

                                                                                                                  tAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑‑‑TTTTTTTTTTTTTTAAAAAAAATAAAATTTTTTTTATTaa         >  2:1556564/1‑128 (MQ=255)

                                                                                                                       aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCT‑TTTTTTTTTTTTTTTTAAAAAAAAAAAAATTTATTTTATTaaaat      >  2:3326155/1‑126 (MQ=255)

                                                                                                                       aaTTTTTAATTTTAATAAATTATTTATTTTTAAAATTTTTAAATTTTTTTAAAATTATTATATTTTAAAAAATTTTTTTTTGT‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACg      <  1:1212126/128‑1 (MQ=255)

||

ATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAATTTTATGCTCG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATA  >  CP016816/420033‑420283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`