BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,163	2 bp→TT	intergenic (‑446/+396)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,163	0	A	T	99.7%	5125.1 / NA	1680	intergenic (‑446/+397)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (1/4); new base T (869/802); total (873/807)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,164	0	A	T	98.8%	4508.0 / NA	1669	intergenic (‑447/+396)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (2/18); new base T (863/786); total (865/804)
Fisher's exact test for biased strand distribution p-value = 1.36e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1686 total aligned reads displayed.

ATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTA  >  CP016816/420038‑420287

||

   cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                                <  2:2389288/128‑1 (MQ=255)

    attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                               >  1:1209292/1‑128 (MQ=255)

     ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                              >  2:1126885/1‑128 (MQ=255)

      tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                             <  2:4029273/128‑1 (MQ=255)

       aatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTg                                                                                                                            <  2:4503249/127‑1 (MQ=255)

        atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                           >  1:4087201/1‑128 (MQ=255)

         tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                          <  2:3392603/128‑1 (MQ=255)

          aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAat                                                                                                                         >  2:3575102/1‑128 (MQ=255)

            ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                       >  1:4144160/1‑128 (MQ=255)

             tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAATAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                                      <  1:3997262/128‑1 (MQ=255)

              ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                                     >  2:2091466/1‑128 (MQ=255)

                aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTAAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAACACTTTTTTAAAAAATTTTTAATTTTAATAACTTAattattat                                                                                                                   >  2:2919072/1‑126 (MQ=255)

                   ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                                >  2:1400949/1‑128 (MQ=255)

                    tcATATATCTAAGTTTATAAAATTATTTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                               <  2:2173381/128‑1 (MQ=255)

                    tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAGTGAATTATTTTTCaa                                                                                                               <  2:4208900/128‑1 (MQ=255)

                    tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                               >  1:2513382/1‑128 (MQ=255)

                    tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATAATAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                               <  2:3053819/128‑1 (MQ=255)

                     cATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTCTTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaaa                                                                                                              <  1:4224515/128‑1 (MQ=255)

                       tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                            >  1:1664345/1‑128 (MQ=255)

                        aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                           >  1:55376/1‑128 (MQ=255)

                        aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                           <  1:1457254/128‑1 (MQ=255)

                         aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                          <  1:171307/128‑1 (MQ=255)

                          aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                         >  1:2151254/1‑128 (MQ=255)

                          aTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                         <  1:1801767/128‑1 (MQ=255)

                            cTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaa                                                                                                       <  2:1753284/128‑1 (MQ=255)

                              aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                     <  1:3086157/128‑1 (MQ=255)

                              aaGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAat                                                                                                     <  1:662100/128‑1 (MQ=255)

                               aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                    <  1:4340063/128‑1 (MQ=255)

                               aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                    <  2:134128/128‑1 (MQ=255)

                               aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                    <  2:4436131/128‑1 (MQ=255)

                                gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                                      <  1:1375159/125‑1 (MQ=255)

                                 tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                                  >  2:1654790/1‑128 (MQ=255)

                                  ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                    <  2:1920074/125‑1 (MQ=255)

                                    cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                               >  1:2045537/1‑128 (MQ=255)

                                     tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                              >  2:2522051/1‑128 (MQ=255)

                                      aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTTTTAAATTATTTTCa                                                                                             >  1:1562062/1‑128 (MQ=255)

                                      aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCa                                                                                             <  2:2446296/128‑1 (MQ=255)

                                       aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                            >  1:3934983/1‑128 (MQ=255)

                                       aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                            <  2:1490937/128‑1 (MQ=255)

                                         attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                          >  1:334849/1‑128 (MQ=255)

                                           tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                                        >  2:3131683/1‑128 (MQ=255)

                                            attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                                       >  2:10572/1‑128 (MQ=255)

                                              tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTa                                                                                     <  2:2811487/128‑1 (MQ=255)

                                               ttATCATTTAAAAAAATAATACTTTTATTAGTTGATTTATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑t                                                                                   >  2:1581147/1‑128 (MQ=255)

                                               atATCATTTAAAAAAATAATACTTTTATTAGTTGATATTTTAGTAAACTTCTAGCAGTTTTTTATAAAATTTTTAATTTTAATTACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑t                                                                                   <  1:794428/127‑1 (MQ=255)

                                                 aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCt                                                                                 <  1:1000320/128‑1 (MQ=255)

                                                 aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCt                                                                                 <  1:3789637/128‑1 (MQ=255)

                                                     tttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTa                                                                             >  2:4094541/1‑128 (MQ=255)

                                                      ttAAAAAAATAATGCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTaa                                                                            >  2:1277615/1‑128 (MQ=255)

                                                       tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAAt                                                                           >  1:1106278/1‑128 (MQ=255)

                                                             aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAAttt                                                                     <  1:996579/128‑1 (MQ=255)

                                                             aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAAttt                                                                     <  1:2745269/128‑1 (MQ=255)

                                                             aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAAttt                                                                     <  2:691675/128‑1 (MQ=255)

                                                             aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAAttt                                                                     >  2:2977025/1‑128 (MQ=255)

                                                             aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAAttt                                                                     <  2:2729406/128‑1 (MQ=255)

                                                             aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTAAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAAttt                                                                     <  1:2920838/128‑1 (MQ=255)

                                                               taataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCTATTTTa                                                                   >  1:3625724/1‑128 (MQ=255)

                                                                  taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGc                                                                >  2:1208247/1‑128 (MQ=255)

                                                                     ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑g                                                           >  2:378704/1‑128 (MQ=255)

                                                                     ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATAAATTTTATGCT‑C‑g                                                           >  2:4275621/1‑128 (MQ=255)

                                                                     ttatattatTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑g                                                           <  1:3502418/125‑1 (MQ=255)

                                                                      tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑t                                                       >  2:4569789/1‑128 (MQ=255)

                                                                      tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑t                                                       >  2:3874347/1‑128 (MQ=255)

                                                                      tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑t                                                       >  2:3487536/1‑128 (MQ=255)

                                                                       ttattaGTTGATATATTAGAAAACTACTAGCCCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGAT‑C‑t‑‑‑tt                                                      >  1:2275625/1‑128 (MQ=255)

                                                                        tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTTTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGAT‑C‑t‑‑‑ttt                                                     >  2:2676428/1‑128 (MQ=255)

                                                                        tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatt                                                                                                    <  2:1842242/87‑1 (MQ=255)

                                                                        tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑ttt                                                     >  1:3577071/1‑128 (MQ=255)

                                                                              ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑tattttttt                                               >  1:2410570/1‑128 (MQ=255)

                                                                               tGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑ttttttttt‑t                                             >  1:3865387/1‑128 (MQ=255)

                                                                                   atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAAAAACTGAATTATTTTTCAAATTTTTAAATTATTTTCAAATCATAA‑TCTATTAATCAATATTATGAT‑C‑G‑‑‑tattttttt‑tattt                                         >  1:908607/1‑128 (MQ=255)

                                                                                    tatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGATTTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑ttttttttt‑tttttt                                        >  2:3727934/1‑128 (MQ=255)

                                                                                      ttAGATAAATAATAGAAATTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTGTTAAAATTTTTTAAAATCATTA‑TATATTAATCAATTTTATGCT‑C‑G‑‑‑ttttttttt‑tttttttt                                      <  2:4144160/128‑1 (MQ=255)

                                                                                        aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑ttttttttt‑tttttaaaaa                                    >  2:2001473/1‑123 (MQ=255)

                                                                                           aaaatacaataaCTTTTTTAAAAAATTTTTTATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTTATTTCAAATAATTA‑TCTATTAATAAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTACaaa                                 <  1:3183473/117‑1 (MQ=255)

                                                                                           aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑ttttttttt‑tttttttttaaaa                                 >  2:331012/1‑123 (MQ=255)

                                                                                           aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTAaaaa                                 >  2:510569/1‑128 (MQ=255)

                                                                                            aaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑‑‑TTTTTTT‑TTTTTTTTACAAAAat                              >  1:2830999/1‑128 (MQ=255)

                                                                                            aaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATAGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTaaaaaa                                >  2:2588731/1‑128 (MQ=255)

                                                                                             actactAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTTaaaaaa                               >  2:2303858/1‑128 (MQ=255)

                                                                                              ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑T‑‑‑TTTTTTTTT‑TTTTTTTTAAAAAAat                              >  2:145260/1‑128 (MQ=255)

                                                                                              ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTATAAAAAat                              >  1:4561530/1‑128 (MQ=255)

                                                                                              ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TATATTAATCAATTTTATGAT‑A‑G‑‑‑ttttttttt‑ttttttaaaaaaaata                              >  2:3218317/1‑118 (MQ=255)

                                                                                              ataatatcaattttttAAAAAATTTTTTATTTTAATAAATCAAATATTTTTCAAATTTTTAAATTATTTTAAAATAAATA‑TATATTAATCAATTTTATGCTCC‑T‑‑‑TTTTTTTTT‑TTTTTTTTACaaaaa                               <  2:785569/118‑1 (MQ=255)

                                                                                               ttaatttaattttttTAAAAATTTTTTATTATAATAACTGTAATTTTTTTAAAATTTTTTAATTTTTTTATAATAAATA‑TTTATTAATAATTTTTTTTCT‑CTT‑‑‑TTTTTTTTT‑TTTTTTTTACAAAAat                              <  2:582002/119‑1 (MQ=255)

                                                                                               tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑‑TTTTTTTT‑TTATTTTAACAAAAatat                            >  2:831280/1‑128 (MQ=255)

                                                                                                actaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑GT‑‑TTTTTTTTT‑TTTTTTTTAAAAAAata                             >  1:4510435/1‑128 (MQ=255)

                                                                                                  tttaatttttttAAAAAATTTTTTATTTTTAAATGTGAATTTTTTTTTTATTTGTTAATTTATTTTATAATCATTT‑TTTTTTAATAAATTTTTTGTT‑G‑G‑‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATaa                          <  1:2336497/122‑1 (MQ=255)

                                                                                                  tataaattttttAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTTTTAAATTATTTTAAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑‑TTTTTTTT‑TTTTTTTTACAAAAATATAat                         <  1:2607712/122‑1 (MQ=255)

                                                                                                  taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑‑‑‑‑‑TTTTTTTT‑TTTTTTTTAAAAaaattaaatt                        >  1:1304137/1‑128 (MQ=255)

                                                                                                   ataaattttttAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTA‑TATATTAATAAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAat                         <  1:489064/123‑1 (MQ=255)

                                                                                                   aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑TTTTTTTTTATTTTTTTTAAAAAAAAATaa                          >  2:362920/1‑128 (MQ=255)

                                                                                                      aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAattta                      >  1:442146/1‑128 (MQ=255)

                                                                                                       cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTAAAAAAAAATAaatttt                     >  1:3451398/1‑126 (MQ=255)

                                                                                                          ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTAAAATCATTA‑TCTATTAATCAATTTTATGGT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAATTTATTTg                  <  1:3742075/128‑1 (MQ=255)

                                                                                                            ttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATATTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTAAAAAAATATAAtttattttat                >  2:892783/1‑128 (MQ=255)

                                                                                                             tAAAAAATTTTTAATTTTAATTAATTAATTATTTTTTAAATTTTTTAATTTTTTTAAAATAATTA‑TATTTTAATTAATTTTATGTT‑G‑G‑‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAATTTATTTGAtt               <  2:225601/128‑1 (MQ=255)

                                                                                                               aaaaaTTTTTAATTTTAATAACTTAATTATTTTTCAAAATGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATGATTTATTTGATTaa             <  2:3954886/128‑1 (MQ=255)

                                                                                                                aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑GTTTTTTTTTTTT‑TTTTTTTTAAAAAAATATAATTTATTTGAtt               >  2:4346548/1‑128 (MQ=255)

                                                                                                                 ataTTTTAATTTAATATAA‑TTAATTTTTTTTAAAATT‑TTAAATTTTTTTTAAATTATTATTATTTTAATAAATTTTTT‑‑T‑‑‑‑‑‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAATTTATTTGATTAAACGtata      <  1:1950159/126‑1 (MQ=255)

||

ATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTA‑TCTATTAATCAATTTTATGCT‑C‑G‑‑‑TTTTTTTTT‑TTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTA  >  CP016816/420038‑420287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`