Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816437,8802.T36.4% 161.6 / inf 209intergenic (‑184/+238)JCVSYN2_01985/JCVSYN2_01990glycosyl transferase/ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base . (57/76);  new base T (41/35);  total (98/111)
Fisher's exact test for biased strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.55e-05

Only 100 of 348 total aligned reads displayed.

TGAAACTTATTTTTGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAATTTAGATTTTTA  >  CP016816/437753‑438006
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tGAAACTTATTTTTGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑g                                                                                                                                                    >  1:1525361/1‑128 (MQ=255)
 gAAACTTATTTTTGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑a                                                                                                                                           >  2:937786/1‑128 (MQ=255)
    aCTTATTTTTGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atat                                                                                                                                        >  2:209469/1‑128 (MQ=255)
       tATTTTTGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGAAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑t‑‑‑‑‑‑‑‑atatata                                                                                                                                     >  2:706666/1‑128 (MQ=255)
        aTTTTTGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatat                                                                                                                                    >  1:837123/1‑128 (MQ=255)
        aTTTTTGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatat                                                                                                                                    >  1:1839604/1‑128 (MQ=255)
         tttttGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatata                                                                                                                                   >  2:87776/1‑128 (MQ=255)
         tttttGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatata                                                                                                                                   >  2:1459828/1‑128 (MQ=255)
         tttttGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatata                                                                                                                                   >  1:1331809/1‑128 (MQ=255)
         tttttGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatata                                                                                                                                   >  2:268866/1‑128 (MQ=255)
          ttttGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTTTATTTTTAAAATAAATATTTTAGTAAATGAAGCAAAGCAAAAACCTCAATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatat                                                                                                                                  >  2:243082/1‑128 (MQ=255)
            ttGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatat                                                                                                                                >  2:1909537/1‑128 (MQ=255)
            ctacaaaaatAAGTTTCATTTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatata                                                                                                                                 >  2:335438/10‑128 (MQ=255)
             tacaaaaatAAGTTTCATTTTTTATTAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGCAAATGAAGCAAATCAAAAACCTCCAAATTACATATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatat                                                                                                                                >  1:100244/9‑128 (MQ=255)
             tGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatata                                                                                                                               >  1:990617/1‑128 (MQ=255)
               tAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatata                                                                                                                             >  1:761081/1‑128 (MQ=255)
                 gTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAATCAAAAACCTACATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑a                                                                                                                       >  2:1052955/1‑128 (MQ=255)
                  ttttaatattCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTTTATTTTTAAAATAAATATTTTATGAAATGAAGCAAAGCAAAAACCTCCATATAACCTATAGAG‑‑‑‑ATA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑at                                                                                                                      <  2:778203/120‑1 (MQ=255)
                   tGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑ata                                                                                                                     >  1:361924/1‑128 (MQ=255)
                    gTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atat                                                                                                                    >  1:1188203/1‑128 (MQ=255)
                    gTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atat                                                                                                                    >  2:731374/1‑128 (MQ=255)
                    gTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atat                                                                                                                    >  2:1833539/1‑128 (MQ=255)
                     tttttttGA‑TTTTTAATAAATATTTTAAATATAAA‑TTTTATTTTTAAAATAAATATTTTTTTAAATGAATCACAGCTAAAAACTCCATATTAAATATATAG‑‑‑‑TTA‑TT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atata                                                                                                                   <  1:142118/124‑1 (MQ=255)
                     tAAGTTTTA‑TTTTTAATAAATATTATATATAAAAA‑TTGTATTTTTAAAATAAATATTTTATGAAATTAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atata                                                                                                                   <  1:282909/128‑1 (MQ=255)
                     tAAGTTTAA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATATTATGAAATGAAGCAAAGCAAAAAACTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atata                                                                                                                   <  2:1522940/128‑1 (MQ=255)
                      aaGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatat                                                                                                                  >  2:1368390/1‑128 (MQ=255)
                       aGTTTCA‑TTTTTAATAAATATTATAAATAAAAA‑TTTTATTTTTAAAATAAATATTTTAGGAAAAGAATCAAACAAAAAAACTCCATATTACCTATAGAG‑‑‑‑ATA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatata                                                                                                                 <  2:1284648/128‑1 (MQ=255)
                        ttttAA‑TTTTTAATAAATATTATAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatatat                                                                                                                <  1:629131/127‑1 (MQ=255)
                        gTTTTA‑TTTTATATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTTTGAAATGAAGCAAAGCTAAAACATCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatatat                                                                                                                <  1:236878/128‑1 (MQ=255)
                         tttCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatatata                                                                                                               >  1:1208176/1‑128 (MQ=255)
                         tttAA‑TTTTTAATATATATTAGAAATAAAAA‑ATGTATTTTTAAAATAAATATTTTATTAAATGAATCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatatata                                                                                                               <  2:442061/128‑1 (MQ=255)
                           tCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCAATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatatatata                                                                                                             <  1:531286/128‑1 (MQ=255)
                                ttttATTAATTTTTAGAAATAAAAATTTTTATTTTTAAAA‑AAATATTTTATCAATTGAAGCAAAGCAAAAAAATCAATATTATATATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatatatata‑‑tata                                                                                                       <  1:213342/128‑4 (MQ=255)
                                   atataaattTTATATATAAATT‑TTTTTTTTTTAAAATATATTTTTTTTTATATTAAAAAAATAAAAAAACTATATATTATCTATAGAT‑‑‑‑TTT‑TT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatatatata‑‑tatatat                                                                                                    <  1:344848/126‑7 (MQ=255)
                                    aataaataTTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatatatata‑‑tatatatg                                                                                                   >  1:423550/1‑121 (MQ=255)
                                     ataaataTTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAAACTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑‑‑ATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATc                                                                                          <  2:664117/128‑1 (MQ=255)
                                      taaataTTATAAATAAATA‑TTTTATTTTTTAAATAAATATTTTATTATATGAAGATAATCTAAAAACTCTATATTATCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatatatata‑‑tatgcaagcg                                                                                                 <  1:1708290/128‑10 (MQ=255)
                                        tatatTAGATATATTAA‑TTTTATTTTTATAAAAAATATTTTATTATATTAATAAAAAAAAAAAAATCAATATATTATATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑ttatatatatatatat‑‑‑‑atatatatata‑‑tatatatgcaag                                                                                               <  2:1207549/127‑12 (MQ=255)
                                        aataTTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCa                                                                                         >  1:1794365/1‑128 (MQ=255)
                                         atatAATAAATATAAA‑TTTTATTTTTAAAATAAATATTTTATTAAATGAATAAAATAAAAAACATCCATATTAAATATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑atatatatatatatat‑‑‑‑atatatatata‑‑tatatatgcaagc                                                                                              <  2:436647/128‑13 (MQ=255)
                                           ataatataTAAAAA‑TTGTATTTTTAAAATATATATTTTATTATATTAATAAAATAAAAAAAATCAATATTTTATATAGAG‑‑‑‑ATA‑ATTA‑‑‑‑‑‑T‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAg                                                                                        <  1:1084846/121‑1 (MQ=255)
                                           atTAGAAAAAAAAA‑TTGTATTTTTAAAATAAATATTATAGGAAATCAAGCATAGCAAAAACCTCAATATTACCTATAGAG‑‑‑‑ATA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑‑‑ATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATa                                                                                    <  2:881761/128‑1 (MQ=255)
                                            ttATAAATAAAAA‑ATGTATTTATAAAAAAAATATTTTACCAAATCAACCAAACAAAAAACCTCCAAATTACCAATAGAT‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑AAATATAAATA‑‑T‑‑‑‑‑‑GCAAGCGATCACCAt                                                                                     <  1:1457719/128‑1 (MQ=255)
                                                aaaTAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGAATaaaa                                                                                 >  2:844143/1‑128 (MQ=255)
                                                  aTAAAAA‑TTGTATTTTTAAAATAAATATTTTATTAAATCAATCAAATCAAAAACCTCCATATTAACTATAGAG‑‑‑‑TTA‑ATTATA‑‑‑‑T‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATaa                                                                                   <  1:937786/128‑1 (MQ=255)
                                                  aTAAAAA‑TTGTATATTTATAATAAATATTTTATTAAATTAAGAAAATCAAAAACCTCCATATTACATATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GAT‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATaaaa                                                                                 <  2:1818707/128‑1 (MQ=255)
                                                    aaaaa‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATAtg‑‑t‑‑‑‑‑‑g                                                                                                   >  2:82796/1‑106 (MQ=255)
                                                       aa‑TTTTATTTTTAAAATAAATATTTTATGAAATCAATCAAATAAAAAACATCAATATTAACTATAGAG‑‑‑‑TTA‑ATTATA‑‑‑‑T‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATgg                                                                              <  1:1276148/128‑1 (MQ=255)
                                                            gTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCTATCAGAATAAAATTGTGaa                                                                          >  1:88066/1‑128 (MQ=255)
                                                              atctttAAAATATATATTTTAGGAAATGAATCAAAGAAAAAAACTCCATATTATCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑‑‑‑‑ATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCt                                                                <  1:15497/125‑1 (MQ=255)
                                                              aTTTTTATAATATATATTTTATTAAATTAATCAAATAAAAAAAATCCATATAATATATAGAGATATTTATATA‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGaa                                                                          <  2:601852/128‑1 (MQ=255)
                                                              aTTTTTAAAATATATATTTTATTAAATTAATATAAGAAAAAACATCAATATTATCTATAGAG‑‑‑‑TTA‑ATTATA‑‑‑‑T‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAAt                                                                        <  1:1571010/128‑1 (MQ=255)
                                                               tttttAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GAT‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGAGATCAGCAAAAAATGGTGTAATTTa                                                                     >  1:1790298/1‑128 (MQ=255)
                                                               tttttAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGCTCAGCATAAAATGGTGAAATTTAGa                                                                   >  2:1229250/1‑128 (MQ=255)
                                                                ttttAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGCTGAGCATAAAATGGTGAAtttt                                                                      >  2:1327749/1‑128 (MQ=255)
                                                                ttttAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑A‑‑‑‑‑‑ACAAGCTATCAGCATAAAATAGTCAAATTTAGAt                                                                  >  1:690364/1‑128 (MQ=255)
                                                                 tttAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAtatata                                                                     >  2:1695091/1‑128 (MQ=255)
                                                                   tAAAATAAATATTTTAGGAAATGAAGCACAGCAAAAAACTCCATATTAGCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑‑‑ATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAg                                                             <  2:1390874/128‑1 (MQ=255)
                                                                   tAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATTTATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATACAATGGTGAATTTTAGATCTg                                                               >  2:382636/1‑128 (MQ=255)
                                                                    aaaaTAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑AAAAGCTATCAGAAAAAAATTGTTATATATATATATGa                                                              >  1:194876/1‑128 (MQ=255)
                                                                      aataaataTTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑‑‑ATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGCTCAGCATAAAATGTTGAATGTTAGATCAGATTTg                                                          >  2:460465/1‑128 (MQ=255)
                                                                       ataaataTTTTAGTAAATTAATAAAATCAAAAAACTCCATATTACATATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGtt                                                           <  2:1675650/128‑1 (MQ=255)
                                                                          aataTTTTATGAAATGAATCAAATATAAAACCTCCATATTAACTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGt                                                            <  2:292268/128‑1 (MQ=255)
                                                                           atatTTTAGTAAATTAATAAAATAAAAAAAATCAATATTAACTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATATAT‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAg                                                             <  2:1976680/128‑1 (MQ=255)
                                                                           atatTTTAGCAAATCAACCAAACAAAAAACCTCCATATTATCTCTAGAG‑‑‑‑TTA‑TT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGtt                                                           <  2:556253/128‑1 (MQ=255)
                                                                             atTTTAGTAAATTAATCATATCTAAAACCTCCATATTACCTATAGAG‑‑‑‑TTT‑ATTATATATAT‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAg                                                             <  2:16289/128‑1 (MQ=255)
                                                                              ttttAGGAAATGAAGCAAAGCAAAACCCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATATATACATATATATATATCT‑‑‑‑‑‑GCAAGCGATAAGCATAAAAAGTTGAAATTTAGATCTGAGTTg                                                          >  1:373440/1‑128 (MQ=255)
                                                                               tttAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GAT‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCTATCAGAATAAAATGGTGAAATTTAGATCTGAGTTGGTcat                                                     >  2:1789219/1‑126 (MQ=255)
                                                                                ttAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCATCATAAAATGGTGAAATTTAGATCTGAGTTGCTCTTaaa                                                  >  2:1133507/1‑128 (MQ=255)
                                                                                  aGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCTATCAGCATAAATAGGTGATATTTAGATCTGAGTTTGTCTTa                                                    >  1:1954344/1‑128 (MQ=255)
                                                                                   gtaaaTGAAGCAAAGCAAAATCCACCATATTACATATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATATATATATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTc                                                       <  2:1536964/126‑1 (MQ=255)
                                                                                      aaTGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑AAAAGCGATCAGAATAAAATGGTGAAATTGATATCTGTGTTTGTCTTAAAtt                                                >  1:917806/1‑128 (MQ=255)
                                                                                        tGAAGAAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGt                                          <  1:731374/128‑1 (MQ=255)
                                                                                           acaaaagcaaaAACCTACATATTATCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGtttt                                       <  1:1105831/125‑1 (MQ=255)
                                                                                            gcaaagcaaaAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTTAGTTGGTATTAAATTTATAGt                                          >  2:1103794/1‑128 (MQ=255)
                                                                                            gcaaagcaaaAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GAAAGCGATCAGAATAAAATGGTGAAATATAGATCTGAGTTTGTCTTAAATTTTTAGt                                          >  2:1605477/1‑128 (MQ=255)
                                                                                             caaatcaaaAACCTCTATATTACCTATAGAG‑‑‑‑TTA‑TT‑‑‑‑‑‑‑‑GAT‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGtttt                                       <  1:1674443/128‑1 (MQ=255)
                                                                                              aaagcaaaAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑TATATAAGCAAGCTATAAGAATAAAATTGTGAATTATAGATCTGAGTTTGTATTAAtttttgtgt                                          >  2:1192868/1‑123 (MQ=255)
                                                                                              aaagcaaaAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑‑‑ATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAATATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTtttattt                                  >  2:1501862/1‑128 (MQ=255)
                                                                                               aacaaaaaaCATCAATATTAACTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGtttt                                       <  2:238520/124‑1 (MQ=255)
                                                                                                agcaaaAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GAT‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GAAAGATATCAGCATAAAATGGTAATATTTATATCTGAGTTTGTCTTATATTTTTTTTTtttat                                    >  1:407365/1‑128 (MQ=255)
                                                                                                agcaaaAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑A‑‑‑‑‑‑TCAAGCGATCATCATAAATTGTTGAAATTTATATCAGAGTTGGTCTTAAATTTGTATTTtttattt                                  >  1:1290942/1‑128 (MQ=255)
                                                                                                  caaaAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGAGATCAGAATAAAATTGTTAAATTTAGATCAGAGTTTTTCTTAAATTTTTAGTTtatat                                    >  1:1418007/1‑128 (MQ=255)
                                                                                                  caaaAACCTCCATATTACCTATAGAG‑‑‑‑TAA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑TAA‑‑‑‑GCTAGCTATCCAAATAAATGTGTGATATTTATATCAGAGTTTGTCTTATATTTATTTTTtttattt                                  >  1:160618/1‑128 (MQ=255)
                                                                                                  aaaaaaaatctctatTAACTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTTGTCTTAAATTTGTAGTTTttatttat                                <  2:1739657/116‑1 (MQ=255)
                                                                                                   aaaaaaCTAAATATTAACTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATAT‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTtttatt                                   <  2:1434621/128‑1 (MQ=255)
                                                                                                    aaaaCCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GATATAT‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTTATATATATATCTGATTTGGTCTTAAATTTTTAGTTtttat                                    >  2:1949086/1‑128 (MQ=255)
                                                                                                    aaaaCCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GAT‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCATCATAAAATTGTGAAATTTAGATCTGTGTTGGTCTTATATTTTTAGTTTttatttat                                >  1:807541/1‑128 (MQ=255)
                                                                                                         ctctATATTACATATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTtat                         <  1:24858/128‑1 (MQ=255)
                                                                                                         ctacaaatTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTtat                         <  2:631489/122‑1 (MQ=255)
                                                                                                         cTCAATATTACCTATATAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑T‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTtat                         <  2:49046/128‑1 (MQ=255)
                                                                                                          tCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATATATAGTTtata                        >  1:1744151/1‑128 (MQ=255)
                                                                                                             atatTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGAATAAAATGGTGAAATTTAGATCTGAGTTTGTCTTAAATTTGTTGTTTTTATTTATATTTTATaaat                     >  1:49046/1‑127 (MQ=255)
                                                                                                                ttACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GAT‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑TCAAGCGATCATAATAATATTGATAAATATATATGTGATTTGATCATAAATTTTTATTTTTTATTTATATTATATaattt                    >  2:1417625/1‑125 (MQ=255)
                                                                                                                  aCCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTtaat                >  1:1892705/1‑128 (MQ=255)
                                                                                                                       taGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGATTTGGTATTAAATTTGTAGTTTTTATTTATAGTTTATAAATTTAATTAttt           >  2:1765763/1‑128 (MQ=255)
                                                                                                                         gag‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑GAT‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTTAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATATTTTATAAAGTTTATAAttt           >  1:650391/1‑128 (MQ=255)
                                                                                                                                ttA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑TCAAGCGATCAGCATAAAATTGTGAAATTTATATCTGAGTTTGTCTTAAATTTGTATTTTTTATATATATTTTATAAATTTAATAATTTATAtt      >  1:1389858/1‑128 (MQ=255)
                                                                                                                                    aT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAATTTAGAttttt   <  2:1873220/128‑1 (MQ=255)
                                                                                                                                     t‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAATTTAGATTTTTa  <  2:363751/128‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGAAACTTATTTTTGTAGTTGTAAGTTTCA‑TTTTTAATAAATATTAGAAATAAAAA‑TTGTATTTTTAAAATAAATATTTTAGGAAATGAAGCAAAGCAAAAACCTCCATATTACCTATAGAG‑‑‑‑TTA‑AT‑‑‑‑‑‑‑‑G‑‑‑‑‑‑‑‑ATATATATATATATAT‑‑‑‑ATATATATATA‑‑T‑‑‑‑‑‑GCAAGCGATCAGCATAAAATGGTGAAATTTAGATCTGAGTTGGTCTTAAATTTGTAGTTTTTATTTATAGTTTATAAAGTTAATAATTTAGATTTTTA  >  CP016816/437753‑438006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑