Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2500T.59.9% 172.6 / inf 524intergenic (+214/‑328)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (112/88);  new base . (227/72);  total (342/182)
Fisher's exact test for biased strand distribution p-value = 3.90e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 629 total aligned reads displayed.

ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGAAAAAATAT‑TGTTAAAA‑GTAATACTAAAATTATTTAATGTTAATTAAAA  >  CP016816/165123‑165376
                                                                                                                                 |                                                                                                                                   
ataataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑t                                                                                                                                     >  2:1681390/1‑128 (MQ=255)
  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑ttt                                                                                                                                   >  2:855416/1‑128 (MQ=255)
  aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttt                                                                                                                                   >  2:901154/1‑128 (MQ=255)
  aataTTTAGTTTAATTAATTTATCGCACTTGTTTTTATTATTAATAAACT‑AAAAATATTATATTTTTAGATAATTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGC‑ttt                                                                                                                                   <  2:1484504/128‑1 (MQ=255)
  aataTATAGTTTCATTAATTTATCGCACTTTTATGAATTAGTATGAAACT‑AAAACTATTATCTTTATAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAAATATAACTTGAAAAAAATAAC‑ttt                                                                                                                                   >  1:406186/1‑128 (MQ=255)
   atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttt                                                                                                                                  >  2:537558/1‑128 (MQ=255)
     atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttt                                                                                                                                >  2:234922/1‑128 (MQ=255)
             tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttttttttttt                                                                                                                        >  2:972819/1‑128 (MQ=255)
             tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGAAAAAAATAGC‑tttttttttttttt                                                                                                                        >  1:1259287/1‑128 (MQ=255)
              cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttt                                                                                                                       >  1:1668756/1‑128 (MQ=255)
                 tAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttattttt                                                                                                                    >  1:883401/1‑128 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttttatatt                                                                                                                 >  1:1480066/1‑123 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTtataa                                                                                                                 >  2:1143323/1‑127 (MQ=255)
                     ttATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttttttataa                                                                                                                >  2:1577370/1‑124 (MQ=255)
                       atccgaCTTGTATTCAATAGTAACCAAAT‑AAAACAATTTTCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAAATAAATTGAAATTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATg                                                                                                             <  1:1874698/123‑1 (MQ=255)
                         cGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAAttta                                                                                                           >  1:478287/1‑125 (MQ=255)
                             cTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTtataattttaattt                                                                                                        >  1:285227/1‑118 (MQ=255)
                             cTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAat‑a                                                                                                      >  1:450138/1‑128 (MQ=255)
                              ttGTATGAATTAGTAAGAAACT‑AAAACTATTATTTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTGAAat‑at                                                                                                     >  2:1204807/1‑128 (MQ=255)
                              ttGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttttttttaatttaaaaat                                                                                                       >  2:648468/1‑116 (MQ=255)
                              ttGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAATAATGTAAat‑a                                                                                                      >  2:1846739/1‑128 (MQ=255)
                              ttGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAAtttaaaatt                                                                                                      >  2:517410/1‑120 (MQ=255)
                               tGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTTTaaattgaaaaa                                                                                                      >  2:1596155/1‑119 (MQ=255)
                                ttattaattattaatTAACT‑AAAACTATTATTTTTTTTTTTATTTTTTTTATTTTGAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATA‑‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAAtt                                                                                                <  2:1913644/127‑1 (MQ=255)
                                gTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTAAAAT‑ATaaa                                                                                                  >  1:771311/1‑128 (MQ=255)
                                  aTGAATTATTAATAAAAT‑AAAACTATTATCTTTTTATATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAAt                                                                                                 <  2:1715275/128‑1 (MQ=255)
                                   ttaaTTATTAATAAAAT‑AAAACTATTATTTTTTTATATATTTTTTTTTTATGTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAAtt                                                                                                <  1:1628037/126‑1 (MQ=255)
                                   ataaTTATTTATAAACTAAAAAATATTA‑CTTTTTATATTTTTTTTTTTTATTAAAATTTTTTAAAAAATAATTTTAAATTGTATAAAAAAGT‑TTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAAt                                                                                                 <  1:1231034/126‑1 (MQ=255)
                                     aaTTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGATCTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAAAGTAAAA‑ATTAAtttt                                                                                              >  2:1041819/1‑128 (MQ=255)
                                     aaTTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGAAATT‑AAAATttttt                                                                                             >  1:357171/1‑127 (MQ=255)
                                        tAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAAT‑ATAAATTTTAgtt                                                                                          >  2:684078/1‑126 (MQ=255)
                                         aGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTTTAATTAAAAT‑ATAAATTTAAgt                                                                                           >  1:254986/1‑127 (MQ=255)
                                          ttAAGAAAAT‑AATAATATTTTTTTTTTAGATATTTTTTGTTATTTCAAATTTTTAAAAAATTAATTTTAACATGTTAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTAt                                                                                        <  2:119189/127‑1 (MQ=255)
                                          gTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTT‑‑TAATTTAAAT‑ATAAATTTTattttt                                                                                        >  1:1003842/1‑123 (MQ=255)
                                           tAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGTTAttt                                                                                      >  1:1201466/1‑128 (MQ=255)
                                            aaGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTA‑AATTTAAAT‑ATAAATTTtatttatt                                                                                       >  2:1743069/1‑128 (MQ=255)
                                            aaGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAATT‑AAAAATTTtatttatt                                                                                       >  2:577064/1‑128 (MQ=255)
                                               aaact‑aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTAT‑‑TTTAAAT‑ATAAATTTTATGTAtttttt                                                                                   >  1:1722134/1‑126 (MQ=255)
                                               aaact‑aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAAT‑ATAAATTTTAGGTAttttt                                                                                    >  2:1561626/1‑127 (MQ=255)
                                               aaact‑aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAAATTAAAAT‑AAAAATATTaataattttt                                                                                    >  2:1406378/1‑119 (MQ=255)
                                                  ct‑aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGAAAAT‑ATAAATTTTATTTATttttttta                                                                                >  1:1895600/1‑126 (MQ=255)
                                                  ct‑aaaactaTTATCTTTTTAGATATTATTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAAT‑ATAAATTTTAGGTttttttttt                                                                                 >  2:1875588/1‑127 (MQ=255)
                                                       aatttttatTTTTTTATATATTTTTTTTATATTTAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAAT‑TT‑TTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑a                                                                            <  2:1429980/123‑1 (MQ=255)
                                                        actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGTAAAT‑ATAAATTTTAGTTATtttttttttaa                                                                             >  2:1632502/1‑123 (MQ=255)
                                                        actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAAT‑ATAAATTTTATTTATTTTTTTTTAT‑aa                                                                           >  2:773746/1‑128 (MQ=255)
                                                         ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTGAAAT‑ATAAATTTTATTTAATTTGTTTTAT‑aatt                                                                         >  2:925561/1‑126 (MQ=255)
                                                         attttttttttttATTTATTTTTTTTTTATTTAAATTTTTTTAAAATTTATTTTTAATTTTTAAAAAT‑TT‑TTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAg                                                                          <  2:274664/120‑1 (MQ=255)
                                                          tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGAAAAT‑ATAAATTTTATTTATTTTTTTTTAT‑AATTa                                                                        >  2:48801/1‑128 (MQ=255)
                                                           ttttattttttATTTTTTTTTTTTTTATTTAAATTTTTTATAAATTTATTTGAAATTGTAAAAAATA‑T‑ATTTTTTTTTTTTTTTTTTATAATTGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTa                                                                        <  2:1355365/122‑1 (MQ=255)
                                                           attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTAAAAT‑AAAAATTTTATTTATTTTGTTGTAT‑AAgat                                                                        >  2:1766161/1‑126 (MQ=255)
                                                           attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTATATAATGAAAAT‑ATAATTTTTAGGAATTTTGTTTTCT‑attta                                                                        >  1:13502/1‑124 (MQ=255)
                                                            ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAATATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAAA‑AAAAATTTTTGttttttttttttatatgttaa                                                                       >  1:530366/1‑117 (MQ=255)
                                                            ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTGAAAT‑ATAAATTTTATGTATTTTTTTTTAT‑AATTAta                                                                      >  2:1106959/1‑127 (MQ=255)
                                                             tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TATTGTAATT‑ATAAATTTTAGTTATTTTTTTTTAT‑aatataa                                                                      >  1:427414/1‑123 (MQ=255)
                                                               tatttttATATATTTTTTGTTATTTCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTa                                                                    <  1:1752306/126‑1 (MQ=255)
                                                               tCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATATTGTATAT‑ATAATTTTTAGTTATTTTTTTTTAT‑aattttttt                                                                    >  2:353021/1‑121 (MQ=255)
                                                                   tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTAT‑TAATTAAAAT‑ATAAATTTTATTTTTTTTGTTTTAA‑AATTATCTataaa                                                                >  1:985957/1‑128 (MQ=255)
                                                                   tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAAT‑ATAAATTTTATTTATTTTTTTTACT‑AATTATATaaaaaa                                                               >  1:1158483/1‑128 (MQ=255)
                                                                        ttatTTTTTTTTTATGTAAATTTTTAAAAATTTTATTTTTAATTGTAAAAAA‑ATT‑GTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAg                                                           <  1:1561626/127‑1 (MQ=255)
                                                                          atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAAT‑ATAAATTTTATTTTTTTTTTTTTAT‑AATTATAaaaaaaaaaaaaa                                                         >  1:1605013/1‑123 (MQ=255)
                                                                           ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGTTATTTTTTTTTAT‑AAGTAaaaaaaaaaaaaaaaa                                                        >  2:245538/1‑128 (MQ=255)
                                                                            tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATATTGTAAAT‑ATAATTTTTATTTTTTTTTTTTATT‑AATTAAAAAAAAAAATATaaa                                                        >  1:1929747/1‑128 (MQ=255)
                                                                             ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATTAAAAT‑ATAAATTTTATTTTTTTTTTTTTAT‑AATTTTTTAaaaaaaaaaaaat                                                       >  2:196806/1‑127 (MQ=255)
                                                                               tgtttatttaaaaTTTTAAAAAATTTAATTTTAATTTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGaaa                                                  <  2:665128/118‑1 (MQ=255)
                                                                                   atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTT‑‑TAATGAAATT‑ATAAATTTTAGGATTTTTGTTGTAT‑ATGTAAATAAAAAAATATAAA‑Gaaaaaatt                                              >  1:909720/1‑126 (MQ=255)
                                                                                      tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTAAAATGTAAAT‑ATAAATTTTAGGTATTTTGTTGTAT‑AAGTTTAAAAAAAAATATAAA‑GAAAAAAATAt‑t                                          >  2:1947990/1‑128 (MQ=255)
                                                                                       gCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAAT‑ATAATTTTTAGTTTTTTTTTTTTAT‑ATTTATATAAAAAAATATAAA‑TGAAAAAATAT‑Tg                                         >  2:608396/1‑128 (MQ=255)
                                                                                         aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TATTTTATAT‑ATAAATTTTTGTTATTTTTTTGAGA‑TAGTATCTAAAAAATTAGAAA‑GGAAAAATTTT‑TGt                                        >  1:1707676/1‑128 (MQ=255)
                                                                                          atttttttaaaaattaattTTTAATTTTAAAAAAT‑TT‑TTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGAAAAAATAT‑TGtt                                       <  1:818171/117‑1 (MQ=255)
                                                                                           aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTATAATTGAAAT‑ATAATTTTTAGTAATTTTTTTTTCA‑AAGTAAATAAAAAAATAAAAA‑TTAAAAAATAT‑T‑TTaaaa                                   >  1:1060429/1‑128 (MQ=255)
                                                                                            ttttTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATTTAAAT‑ATAAATTTTATTTATTTTTTTTTAT‑ATTTATAAAAAAAAATATAAA‑TTAAAAAATTT‑TTTTa                                      >  2:1468623/1‑128 (MQ=255)
                                                                                               ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGTAAAT‑ATAAATTTTATTTTTTTTTTTGTAT‑AATTATATAAAAAAAAAAAAA‑GAAAAAAATAT‑TTTAaaaa                                   >  1:567035/3‑128 (MQ=255)
                                                                                                 aaaaaaTTAATTTGAACTTGTTAAAAAT‑TT‑ATTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGAAAAAATAT‑TGTTAAAA‑GTa                               <  2:1002984/128‑1 (MQ=255)
                                                                                                     aattaattTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTAAAATTGAAAT‑ATAAATTTTAGTTATTTTTTTTTAT‑AAGTATCTAAAAAAATATAAA‑GTAAAAATAAT‑TTTTAAAA‑TTAATa                            >  1:1528581/1‑128 (MQ=255)
                                                                                                     aattaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAATT‑ATAAATTTTAGTTATTTTTTTTTAT‑AATTAAATAAAAAAATATAAA‑TTAAAAAATAT‑TTTTAAAA‑TTaaaaa                           >  2:70642/1‑125 (MQ=255)
                                                                                                     aattaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTTTTTTTTTTTAT‑AAGATTATAAAAAAATATAAT‑GTAAAAAATAT‑TTTTAAAA‑GTAATaa                           >  2:1658315/1‑127 (MQ=255)
                                                                                                      attaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATGTAAAT‑ATAAATTTTATTTTTTTTTTTGTAT‑AATTATAAAAAAAAATAAAAA‑GTAAAAAATAT‑TTTTAAAA‑TTAATa                            >  1:684786/1‑128 (MQ=255)
                                                                                                      attaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAAT‑ATAAATTTTTTGTTTTTTTTTTTAA‑AAGTTTATAAAAAAATAAAAA‑TAAAAAAATAT‑TTTTTAAA‑TTAATaat                          >  1:1110858/1‑126 (MQ=255)
                                                                                                      attaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAAT‑AAAAATTTTAGTTATTTTTTTTTAT‑AATTTTATAAAAAAAAAAAAA‑TTAAAAAATTT‑TTTTaaatttaaaaat                           >  1:1317681/1‑119 (MQ=255)
                                                                                                      attaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTTTGTTTTTTTTTTTAT‑AA‑TTTAAAAAAAAATATAAATGTAAAAAATAT‑T‑TTAAAA‑GTTAttaaaaa                       >  1:1778099/1‑122 (MQ=255)
                                                                                                        taattTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TATTTTAAAA‑AAAAATTTTTTTATTTTTTTTTTAT‑AATTTTATAAAAAAATATAAA‑TAAAAAATTTT‑TTTTaaattaaataataa                         >  2:1926713/1‑117 (MQ=255)
                                                                                                        taattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTATTAATAATGGAAAT‑ATAAATTTTAGGTTTTTTGTTGTCT‑AAGTATATAAAAAATAAGAAA‑GGAAAAAATTT‑TTTTAAAA‑TTAATACTaa                        >  2:1809366/1‑128 (MQ=255)
                                                                                                        taattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTAAAATTTAAAT‑ATAAATTTTAGTTATTGTGTTGAAT‑AAGTATCTAAAAAAAATAAAA‑GGAACAAATAT‑TTTTAACT‑GAAAGACTaaa                       >  2:1254017/1‑128 (MQ=255)
                                                                                                        taattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTTAAAATGGTAATAAAAAATTTTAGG‑ATTTTTTTTTATAAATTTTAAAAAAAAATAAAAA‑GAAAAAAAATT‑TATTaaaataaaaaaaaaa                        >  1:103292/1‑117 (MQ=255)
                                                                                                         aattTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTTTAAATTAAAA‑ATAAATTTTAAATATTTTTTTGAAG‑TATTATTCAAAAAAATATAAA‑ATAAAAAAAATATTTTAAAATGTAATaat                          >  2:1314026/1‑126 (MQ=255)
                                                                                                             tGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TAATGAAATT‑AAAAATTTTTTGTTTTTTTTTTAAA‑AATTATATAAAAAAA‑AAAAA‑AAAAAAAAAAT‑TTTTAAAA‑ATAAAAAAAAAattat                  >  1:617734/1‑128 (MQ=255)
                                                                                                              gAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTTTAATGTAAAT‑ATAAATTTTTTTTTTTTTTTTTTTT‑ATTTATATAAAAAAATATAAA‑TTAAAAAATTT‑TTTTAAAA‑TTAATAATAAAattt                   >  2:411731/1‑127 (MQ=255)
                                                                                                              gAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TAATGTAAAT‑ATAATTTTTAGTTTTTTTGTTGTAT‑ATTTATATAAAAAAATATAAA‑GTAAAAAATAT‑TTTTAAAA‑TTAATAATAAAattat                  >  2:1072436/1‑128 (MQ=255)
                                                                                                              gAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAAATAAAAAA‑ATAAATTTAAAGAATTTTGTTTTTT‑AATTATAAAAAAAAATAAAAA‑AAAAAAAATAT‑TATAAAAA‑TTAATAAAAAAataatt                 >  2:3106/1‑128 (MQ=255)
                                                                                                               aaCTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAAATGTAAAT‑ATAAATTTTTTTTATTTTTTTGTAT‑AATTTAATAAAAAAAATAAAA‑GTAAAAAATAT‑TTTTAAAA‑TTAAAAAAAAAattttt                 >  1:1292470/1‑128 (MQ=255)
                                                                                                                aattGTAAAAAATTG‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGAAAAAATAT‑TGTTAAAA‑GTAATACTAAAATTAttt                <  1:1819285/126‑1 (MQ=255)
                                                                                                                  ttGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATATTGGAATT‑ATAATTTTTTGGTATTTTGTTTTAT‑AAGTTTATAAAAAAATATAAA‑GTAAAAAATAT‑TTTTAAAA‑GTTATAATAAAATTATTTaa              >  2:798954/1‑128 (MQ=255)
                                                                                                                   tGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAAATTTAAAA‑AAAAATTTTATTTATTTTTTTTTAA‑AAATAAAAAAAAAAATATAAA‑TTAAAAAATAT‑TATTAAAA‑TTAAAAAAAAAATTATTAAAt             >  1:935507/1‑128 (MQ=255)
                                                                                                                    tttaaaaaTATC‑TTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGAAAAAATAT‑TGTTAAAA‑GTAATACTAAAATTATTTAAt             <  1:196806/125‑1 (MQ=255)
                                                                                                                    gTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTTTTTTTTTGTAT‑AATTATATAAAAAAATATAAA‑GTAAAAAATAT‑TTTTAAAA‑TTAATAATAAAATTTTTTAAtag           >  2:44050/1‑126 (MQ=255)
                                                                                                                      ataaaaTATT‑TTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGAAAAAATAT‑TGTTAAAA‑GTAATACTAAAATTATTTAATGt           <  1:230890/126‑1 (MQ=255)
                                                                                                                        aaaaTAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAAT‑TTAAATTTTTGGTTTTTTTTTTTAT‑AATTATATAAAAAAATAAAAA‑TGAAAAAATTA‑TTTTTAAA‑GTAATAATAAAATTATTTAATTttaa        >  2:1532309/1‑128 (MQ=255)
                                                                                                                           aaattttttttttttttttttttttATAATTGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGAAAAAATAT‑TGTTAAAA‑GTAATACTAAAATTATTTAATGttaat       <  2:478287/122‑1 (MQ=255)
                                                                                                                            attattttttttttttttttttttATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGAAAAAATAT‑TGTTAAAA‑GTAATACTAAAATTATTTAATGttaatt      <  2:1887852/123‑1 (MQ=255)
                                                                                                                             attttttttttttttttttttttATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGAAAAAATAT‑TGTTAAAA‑GTAATACTAAAATTATTTAATGttaatta     <  1:140821/124‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                   
ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACT‑AAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAAT‑ATAAATTTTAGGTATTTTGTTGTCT‑AAGTATCTAAAAAAATAGAAA‑GGAAAAAATAT‑TGTTAAAA‑GTAATACTAAAATTATTTAATGTTAATTAAAA  >  CP016816/165123‑165376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑