Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,218 A→T intergenic (+182/‑360) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2180AT98.7% 1827.2 / NA 613intergenic (+182/‑360)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (2/6);  new base T (389/216);  total (391/222)
Fisher's exact test for biased strand distribution p-value = 2.93e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 636 total aligned reads displayed.

TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAA  >  CP016816/165093‑165344
                                                                                                                             |                                                                                                                                     
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                     <  2:1136995/128‑1 (MQ=255)
tttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaa                                                                                                                                     >  2:786009/1‑128 (MQ=255)
 ttACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaa                                                                                                                                    >  1:1035655/1‑128 (MQ=255)
 ttACTTTAATAGTATTAATTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaa                                                                                                                                    <  1:1232368/128‑1 (MQ=255)
    cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                 >  1:272731/1‑128 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                              >  1:10544/1‑128 (MQ=255)
       tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                              >  1:1594738/1‑128 (MQ=255)
        aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                            >  1:1002323/1‑128 (MQ=255)
        aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                            <  2:1064796/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:1200387/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  2:832794/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  2:1375708/128‑1 (MQ=255)
           tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:786009/128‑1 (MQ=255)
             tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                       <  2:620491/128‑1 (MQ=255)
                 actttactAGTTGTTAATATTTAGTTTCATTAATTTATCGCACTTGTATGATTTAGTAAGAAACTAAAACTATTATCATTTTAGATATTTTTTGTATATGCAAATTTTTAAAATATTAATTTGAACtt                                                                                                                   <  1:1497685/128‑1 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  1:1101806/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:1960249/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  1:353326/1‑128 (MQ=255)
                   tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATAAATTTGAACTTGt                                                                                                                 <  2:976961/128‑1 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                >  2:1720886/1‑128 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                >  1:469058/1‑128 (MQ=255)
                    ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATATGAACTTGTa                                                                                                                >  1:974890/1‑128 (MQ=255)
                      actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                              <  2:1659694/128‑1 (MQ=255)
                       atAGTTGATAATATTTAGTTTAATTAATTTATCGAACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                             <  1:1258356/127‑1 (MQ=255)
                          gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑t                                                                                                        >  2:1973722/1‑128 (MQ=255)
                           tttataataTTTTTTTTCATTAATTTATCGCAATTTTTTTAATTATTAATAAAATAAAAATTTTATTTTTTTTTTTATTTTTTGTTTATGCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAA‑‑Ta                                                                                                       <  1:629332/125‑1 (MQ=255)
                             gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAA‑‑TAGc                                                                                                     >  2:1256355/1‑128 (MQ=255)
                                 atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑tttt                                                                                               >  1:852958/1‑128 (MQ=255)
                                     ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑tttttttt                                                                                           >  2:746505/1‑128 (MQ=255)
                                      ttgTTTCAATAATTTATCCCCATTGTATTAAATACTAATAAACTAAAACTATTATATTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAA‑‑TAGC‑‑ttttttttt                                                                                          <  1:1679841/126‑1 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑ttttttttttt                                                                                        >  1:1146245/1‑128 (MQ=255)
                                        gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑tttatatttat                                                                                        >  1:994769/1‑126 (MQ=255)
                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAA‑‑TAGC‑‑tttttttttttt                                                                                       >  2:1229962/1‑128 (MQ=255)
                                         tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑tttttttttttt                                                                                       >  1:1905646/1‑128 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAACA‑‑TAGC‑‑ttttttttttttttt                                                                                    >  2:1570562/1‑128 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑ttttttttttttttt                                                                                    >  2:575567/1‑128 (MQ=255)
                                            cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑ttttttttttttttt                                                                                    >  1:1884552/1‑128 (MQ=255)
                                             aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑tttttttttttttttt                                                                                   >  2:936946/1‑128 (MQ=255)
                                              ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAA‑‑TAGC‑‑ttttttttttttttttt                                                                                  >  2:1547683/1‑128 (MQ=255)
                                               tAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TATC‑‑tttttttttttttttttt                                                                                 >  1:202995/1‑128 (MQ=255)
                                                  tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑ttttttttttttttttttata                                                                              >  2:287749/1‑125 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑ttttttttttttttttataattaaaa                                                                         >  2:4886/1‑118 (MQ=255)
                                                       cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTtatattta                                                                         >  1:145168/1‑124 (MQ=255)
                                                        gCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTATAAtttaa                                                                       >  1:1638096/1‑124 (MQ=255)
                                                         caaatGTATTAATTATTAAGAAAATAAAAATATTATATTTTTATATTTTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAA‑‑TA‑‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAata                                                                    <  1:898493/124‑1 (MQ=255)
                                                           cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTtataatttaaatat                                                                     >  2:531489/1‑118 (MQ=255)
                                                            ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTCTTTTTTATAATGGAAATATaa                                                                <  2:691939/128‑1 (MQ=255)
                                                              gTATGAATTAGTAATAAAATAAAAATATTATTTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                 <  1:176934/128‑1 (MQ=255)
                                                               tATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                               <  2:1594073/128‑1 (MQ=255)
                                                                 ataaTTATTAATAAACAAAAAATATTATATTTTTTTATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAA‑‑ATT‑‑CTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                             <  2:1111582/126‑1 (MQ=255)
                                                                    aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAG‑‑ATTATTTTTTTTTTTTTTTATAATATAAATATAAATTTTa                                                          >  2:651687/1‑128 (MQ=255)
                                                                     ttttttataaaataaaaattTTATATTTTTTTTTTTTTTTTTTATATTTAAATTTTTTAAAAATTAATTTTTAATTTTAAAAAATTTTTT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAttt                                                            <  2:1202518/120‑1 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTatt                                                        >  2:1628037/1‑126 (MQ=255)
                                                                      tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTAAAATATAAAtttaattt                                                       >  2:1414373/1‑123 (MQ=255)
                                                                       gtaataaaattaaaattaTATATTTTTATTTTTTTTTTTTTTATTTAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTa                                                      <  2:1690077/122‑1 (MQ=255)
                                                                       aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTattta                                                      >  2:1854861/1‑124 (MQ=255)
                                                                        ttAAGAAAATAATAATATTTTTTTTTTAGATATTTTTTGTTATTTCAAATTTTTAAAAAATTAATTTTAACATGTTAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                     <  2:119189/127‑1 (MQ=255)
                                                                            ttaaataaaaataTTATATTTTTATATATTTTTTTTATATTCAAATTTTTTAAAAAATAATTTTAAATTTTAAAAAA‑‑TA‑‑‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                 <  1:684078/123‑1 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTAAAATTTAATAAAAATTTTaatttttttt                                                 >  2:238965/1‑119 (MQ=255)
                                                                             aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTattaattttt                                                 >  1:1710669/1‑119 (MQ=255)
                                                                               actaaaactaTTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAA‑‑TATC‑‑TTTTTTTATATTTTTTAATAAATGAAATTAAAATTTTATTTATttttttc                                              >  1:1372412/1‑127 (MQ=255)
                                                                               actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TATTTTTTTTTTTTTTATAATTAAAATTTAAATTATATTTATTTtgatgt                                             >  1:526241/1‑128 (MQ=255)
                                                                                ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTc                                            >  2:511321/1‑128 (MQ=255)
                                                                                ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTtgttgta                                            >  2:1496728/1‑127 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTTTTTttttgt                                             >  2:1886597/1‑128 (MQ=255)
                                                                                 taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATATTGGATATTTAAATTTTAGGTATTttgtgtag                                            >  1:1306069/1‑124 (MQ=255)
                                                                                    aactaTTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑TTTTTTTTTTTTATTTATAATGTAAATATAAATTTTAGATATTTTTTTGTATAAg                                        >  2:1474162/1‑128 (MQ=255)
                                                                                     actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTATTTATttttttttaaa                                          >  1:909883/1‑123 (MQ=255)
                                                                                      ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAG‑‑ATTTTTTTTTTTTTTTTTTATAATTAAAATATAAATATAATTTATTTTTTTTTATaat                                        >  2:470916/1‑127 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTTTTTTTTTTTATaat                                        >  1:495497/1‑127 (MQ=255)
                                                                                       tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTTGTTATTTTTTTTTATAATTa                                      >  2:192486/1‑128 (MQ=255)
                                                                                        ttttttttttttAATATTTTTTTTTATTTAAATTTTTTAAAATTTTATTTGAAATTGTTAAAAA‑‑TA‑‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                   <  2:1909210/122‑1 (MQ=255)
                                                                                        tttatTTTTTTATTTATTTTTTTTTATTTTAAATTTTTTATAAATTTATTTTTACTTTTTAAAAA‑‑AAT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                      <  1:43264/127‑1 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGAATTTTGTTTTATAAGTa                                      >  2:1760599/1‑128 (MQ=255)
                                                                                         ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTAta                                    >  2:906199/1‑127 (MQ=255)
                                                                                         ttatCTTTTTAGATAATTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTAAAATTGTAAATAAAAATTTTATGAATttttttttaaatttat                                     >  2:1830141/1‑118 (MQ=255)
                                                                                                tttatttatTTTTTTTTTAATCAAATTTTTAAAAAATAAAATTTAAATTTTTAAAAA‑‑TATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaa                              <  1:1538423/122‑1 (MQ=255)
                                                                                                      ttttttttttttATTTAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAA‑‑TA‑‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaaa                     <  1:575567/126‑1 (MQ=255)
                                                                                                        ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAAAAAAAATTTTATTTAATTTTTTTTAAAATTAAATAaaaaaaaaaaaa                     >  2:271018/1‑128 (MQ=255)
                                                                                                         tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAAAATAAATTTTAGGTATTTTGTTTTATAATTAAATAAAAAAATATaaat                    >  2:414363/1‑127 (MQ=255)
                                                                                                          ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGTAAATTTAAATTTTATGTATTTTTTTTTATATTTATCTAAAAAAATTGaata                    >  2:875507/1‑126 (MQ=255)
                                                                                                           tttttatattgtaaTTTTTAAAAAATTAATTTGTACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                  <  2:1771515/116‑1 (MQ=255)
                                                                                                            ttttattttaaaaTTTTTAAAAAATTAATTTTAAATTTTAAAAAA‑‑AT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                <  1:134740/118‑1 (MQ=255)
                                                                                                            ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTATAATTAAAAAATAAATTTAAGTATTTTTGTTTTCTAATTAAATaaaaaaaaataatttaa                  >  2:431364/1‑118 (MQ=255)
                                                                                                            ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTAAAATTAAATTTAAAATTTTAGTTTTTTTTTTTTAAATGTATAAAAAAAAATAAAAAGaaa                 >  1:405051/1‑128 (MQ=255)
                                                                                                                 tatGTAATTTTTTTAAAAATTAATTTTTACTTGTAAAAAA‑‑TAT‑‑GTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat            <  1:1940464/128‑1 (MQ=255)
                                                                                                                 tatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTAAAATGTAAATATAATTTTTAGTTTTTTTTTTTTTTAATTTTGTAAAATAATATAAAGaaaaaatt            >  2:341128/1‑126 (MQ=255)
                                                                                                                 tatGCAAATTTTTAAAAAATTAAATTTAAATTTTAAAAAA‑‑TTT‑‑GTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat            <  2:1035655/128‑1 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTATATTTATAATATAAATTTTAGGTTTTTTTTTTTATATTTAAATAAAAAAATAAAAAGAAAAAAatt           >  2:672810/1‑127 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATTTATTTTGTTTTATAATTTTATAAAAAAATATAAATAAAAAAatat          >  2:591051/1‑128 (MQ=255)
                                                                                                                   tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑TTTTTTTTTTTTTTTTTATTTTTTAAATATAAATTTTAGTTATTTTGTTTTATAATTATTTAAAAAAATAGAAAGTAAAAAAtttt         >  2:1430439/1‑128 (MQ=255)
                                                                                                                     cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGCTTTTTTTTTTTTTTTTTTTTTATAATGAAAAAATAAATTTTAGTTATTTTTTTTTATAATTATAAAAAAATATATAAAGAaaaaaaat           >  1:244194/1‑126 (MQ=255)
                                                                                                                     cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTTTAATGTAAAAATAAATTTTTTTTATTTTTTTTTAAAATTATAAAAAAAAATaaaaataaaaaataatt        >  1:758164/1‑125 (MQ=255)
                                                                                                                      aaaTTTTTTAAAAATTATATTTTAATTTTAAAAAA‑‑TAT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGt       <  1:425059/128‑1 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTATAATTTAATATAAAATTTTATTTATTTTTTTTAATAATAATAAAAAAAAATAAAAAGAAAAAAatatat        >  1:1736588/1‑126 (MQ=255)
                                                                                                                      aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTTTCTAAGTATCTAAAAAAATAGAAAGTAAAAAATTTTTTTaa    >  1:303621/1‑128 (MQ=255)
                                                                                                                      aaaTTTTAAAAAATTTATTTTAACGTTTAAAAAA‑‑AATC‑‑TTTTTTTTTTTTTTTTTTATAATTGAAATTTTAATTTTTAGTTTTTTTTTGTTTAATTAAAAAAAAAAATTAAAAAGAAAAAAATAtttt       >  1:1611996/1‑126 (MQ=255)
                                                                                                                       taTTTTTAAAAATTAAATTTTAATTTTAAAAAA‑‑TA‑‑‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa    <  1:1828312/127‑1 (MQ=255)
                                                                                                                       atttttAAAAAAATTATTTTTAATTTTAAAAAA‑‑ATT‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa     <  2:1527082/126‑1 (MQ=255)
                                                                                                                        aTTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑‑‑‑‑TTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGTTATTTTTTTTTAAAATTATAAAAAAAAAATTAAATTAAAAAATATTTTTaaaa  >  2:1335875/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                                     
TTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAA‑‑TAGC‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAA  >  CP016816/165093‑165344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: