Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA99.5% 1928.5 / NA 609intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (386/217);  minor base T (0/3);  total (388/221)
Fisher's exact test for biased strand distribution p-value = 4.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 618 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165097‑165347
                                                                                                                             |                                                                                                                              
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                             >  1:1035780/1‑128 (MQ=255)
    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                          >  1:1002323/1‑128 (MQ=255)
    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                          <  2:1347960/128‑1 (MQ=255)
    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                          <  2:882766/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAATCTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                       <  2:729387/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                       <  1:1200387/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                       <  2:83603/128‑1 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                       <  2:1533046/128‑1 (MQ=255)
       tGTATTACGTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                       <  2:918089/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                      <  1:523608/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                      <  2:1844438/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                      >  2:1887887/1‑128 (MQ=255)
         tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                     <  2:620491/128‑1 (MQ=255)
         tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGa                                                                                                                     <  1:983622/128‑1 (MQ=255)
           ttactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAc                                                                                                                   >  2:1861131/1‑128 (MQ=255)
             actttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACtt                                                                                                                 >  2:1477653/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                               >  1:1101806/1‑128 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATAAATTTGAACTTGt                                                                                                               <  2:976961/128‑1 (MQ=255)
                ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                              <  1:460450/128‑1 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAattttaaattttaa                                                                                                             >  1:905869/1‑128 (MQ=255)
                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                            >  2:678136/1‑128 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                         <  2:1788656/128‑1 (MQ=255)
                       ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                       >  1:485980/1‑128 (MQ=255)
                        ttataataTTAATTTTTATTAATTTTTCGCACTTGTATTAATTATTAATAAAATAAAAATATTATCTTTTTAGATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAAAAg                                                                                                      <  2:1641431/126‑1 (MQ=255)
                        tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATat                                                                                                      >  2:1196506/1‑127 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                     >  1:1400069/1‑128 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                  >  2:1961131/1‑128 (MQ=255)
                             atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                 >  2:537558/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                          >  1:429552/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                          >  1:1146245/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAttttttttttt                                                                                          >  2:271157/1‑128 (MQ=255)
                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                       >  2:1216472/1‑128 (MQ=255)
                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                       >  2:783561/1‑128 (MQ=255)
                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGAAAAAAATAGCtttttttttttttt                                                                                       >  1:1259287/1‑128 (MQ=255)
                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                      >  2:575567/1‑128 (MQ=255)
                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                      >  1:889630/1‑128 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                     >  1:1825191/1‑128 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                     >  2:936946/1‑128 (MQ=255)
                                               ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttataa                                                                               >  2:1577370/1‑124 (MQ=255)
                                               ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttataa                                                                               >  2:1723224/1‑124 (MQ=255)
                                                 atccgaCTTGTATTCAATAGTAACCAAATAAAACAATTTTCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAAATAAATTGAAATTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATg                                                                            <  1:1874698/123‑1 (MQ=255)
                                                   gggaagtGTATTAATTTTTTATAAAATAAAAATTTTTTTTTTTTTTTTATTTTTTTTTTTTGCAAATTTTTTAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATgg                                                                           <  1:1887887/122‑1 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAACACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGGa                                                                          >  1:92077/1‑128 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttataattaaaa                                                                           >  2:4886/1‑118 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttttttttatttaa                                                                       >  1:1671730/1‑117 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAataa                                                                     >  1:1307444/1‑127 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAAATAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATAAATTTGAAATTGTAAAAAATAGC‑‑TTTATTTTTCTTTTTTTATAATGTAAAtata                                                                   >  2:300459/1‑128 (MQ=255)
                                                          ttattaattattaatTAACTAAAACTATTATTTTTTTTTTTATTTTTTTTATTTTGAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                               <  2:1913644/127‑1 (MQ=255)
                                                            aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAATATAAAt                                                                >  1:677241/1‑128 (MQ=255)
                                                            aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAttt                                                              >  2:1674078/1‑128 (MQ=255)
                                                             ttaaTTATTAATAAAATAAAACTATTATTTTTTTATATATTTTTTTTTTATGTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                               <  1:1628037/126‑1 (MQ=255)
                                                             tgtattattAATAAAATAAAAATATTATTTTTTTATTTATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTTACTTGTAAAAAAAAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                               <  1:1577370/125‑1 (MQ=255)
                                                             tGAAATATTAATAAACAAAAAATAATATCTTTTTATATATTTTTTGTATTTGTAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAAATTCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                               <  2:1422680/128‑1 (MQ=255)
                                                               aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGATCTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAAAGTAAAAATTAAtttt                                                             >  2:1041819/1‑128 (MQ=255)
                                                                  tAGTAAGAAATATAAAATTTTTTTTTTTTATATTTTTTTTGTATATGCTAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                         <  2:1981406/128‑1 (MQ=255)
                                                                   tttaataaACTAAAACTATTATATTTTTTTATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTTACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                         <  1:1766161/126‑1 (MQ=255)
                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAATATAAATTTTatta                                                         >  2:1404458/1‑125 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTATTTTAAAATTGAAATATAAAattaatgttt                                                       >  1:1236126/1‑121 (MQ=255)
                                                                     ttaaaaaaaaaaaattTTATTTTTTTTATTATTTTTTTTTTATTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                       <  1:419425/119‑1 (MQ=255)
                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTattaattttt                                                   >  1:1710669/1‑119 (MQ=255)
                                                                          aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTATTTTTTTTTTTTATTATAATTTAATAATAAATtatatttaattttt                                                  >  2:1292420/1‑118 (MQ=255)
                                                                            aaaaaaataTTTTATTTTTATTTATTTTTTTTATTTTTAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAAATTGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttgttg                                                <  2:1705957/126‑1 (MQ=255)
                                                                             ttaaaataATATTTTTTTTTATTTTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                               <  2:1555300/126‑1 (MQ=255)
                                                                             taatactaTTATTTTTTTTTTTATTTTTTTTATATGCAAATTTTAAAAAAATTAATTTTAAATTGTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                               <  1:780936/128‑1 (MQ=255)
                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATtttttttt                                               >  2:1127797/1‑126 (MQ=255)
                                                                               aaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTttttgtaa                                             >  2:145903/1‑126 (MQ=255)
                                                                                  ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTATAATTAAAATATAAATATAATTTATTTTTTTTTATaat                                          >  2:470916/1‑127 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAAAGTAAATAAAAATTTTAGGTAttttttggaataatt                                         >  1:378833/1‑117 (MQ=255)
                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTAATTTATTTTGTTTTATAATTAta                                      >  1:284721/1‑127 (MQ=255)
                                                                                      tatTTTTTTTTATATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATATATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                      <  2:124526/128‑1 (MQ=255)
                                                                                      tatTTTTTTATATATTTTTTTTTTTTTCAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATc                                      <  1:623636/128‑1 (MQ=255)
                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTATTTTTTTATTGGAAATATAAATTTTAGGTTTTTTTATGTATAATTaat                                      >  1:1528934/1‑126 (MQ=255)
                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATTTTTTTTTTTTTATAATTAta                                      >  2:659664/1‑127 (MQ=255)
                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTTATTTTTTTTTATAATTAtaa                                     >  1:1058916/1‑126 (MQ=255)
                                                                                        tCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTGTATATATAATTTTTAGTTATTTTTTTTTATaattttttt                                    >  2:353021/1‑121 (MQ=255)
                                                                                           ttttTGATATTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATTTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaa                                  <  1:1456670/128‑1 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTTTTTTATAATTATATaaaa                                 >  2:1605403/1‑128 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATTAATTTTAGGTTTTTTTTTGTCTaatttagataat                                 >  1:351415/1‑118 (MQ=255)
                                                                                            tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTATAATTATATaaaa                                 >  2:1181655/1‑128 (MQ=255)
                                                                                            tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTTTATAAGTATATaaaaaa                               >  1:718897/1‑128 (MQ=255)
                                                                                                 atatTTTTTTTATATGATAATTTTTAAAAAAATAATTTTAACTTTTAAAAAATATGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                           <  2:239949/128‑1 (MQ=255)
                                                                                                 atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTGTTGAATAAGTATCTAAAAAAATAGa                          >  2:691603/1‑128 (MQ=255)
                                                                                                   atTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATATAATGGAAATATAAATTTTAGGTATTTTTTTTTCTAAGTTTCCAAAAAAATAGaa                         >  2:1762687/1‑128 (MQ=255)
                                                                                                    attttttTATATTTAAATTTTTAAAAAATTAATTTTTAAATTTAAAAAAAA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                       <  1:1606461/127‑1 (MQ=255)
                                                                                                     tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATATTGTAAATATAATTTTTATTTTTTTTTTTTATTAATTAAAAAAAAAAATATaaa                        >  1:1929747/1‑128 (MQ=255)
                                                                                                     tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAAAATAAATTTTAGGTATTTTGTTTTATAATTAAATAAAAAAATATaaat                       >  2:414363/1‑127 (MQ=255)
                                                                                                       tttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAATTGAAAATATAAATTTTAGGTATTTTGTTGTCTAATTTTCTAAAAAAATAAAAATGa                     >  2:1501182/1‑128 (MQ=255)
                                                                                                        ttttattttaaaaTTTTTAAAAAATTAATTTTAAATTTTAAAAAAATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                   <  1:134740/118‑1 (MQ=255)
                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAAAATAAATTTAAGTATTTTTGTTTTCTAATTAAATaaaaaaaaataatttaa                    >  2:431364/1‑118 (MQ=255)
                                                                                                           attttaaaaaTTTTTTAAAAATAAATTTTAATTGTTAAAAAATTTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaa                 <  1:1977351/121‑1 (MQ=255)
                                                                                                            ttatttaaaTTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat               <  1:271157/122‑1 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAAAAAAAATTTTAGGTTTTTTTTTGTAAAAGTAAATAAAAAAATATAAAGGAAAAAatta             >  1:562997/1‑126 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATTTATTTTGTTTTATAATTTTATAAAAAAATATAAATAAAAAAatat             >  2:591051/1‑128 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATTATTTAAATATAAATTTTTTTTATTTTTTTTTATAATTATAAAAAAAAAAATAAAGAAAAAAaaata            >  1:849262/1‑127 (MQ=255)
                                                                                                                 cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATTGTAAATTAAAATTTTTGTTTTTTTTTTTTTAATTTTTATAAAAAAATATAAAGAAAAAAATAttt           >  2:1927002/1‑127 (MQ=255)
                                                                                                                  aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAATATAAAATTTTATTTATTTTTTTTAATAATAATAAAAAAAAATAAAAAGAAAAAAatatat           >  1:1736588/1‑126 (MQ=255)
                                                                                                                     ttttTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAT‑‑TGGAAATATAAATTTTAGTTATTTTTTTGTATAAGTATATAAAAAAATTTAATGTAAAAAATATTTTTaaa      >  2:51569/1‑128 (MQ=255)
                                                                                                                     ttttTAAAAAATTAAATTGAAATTTTAAAAAATTTCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa       <  1:233546/128‑1 (MQ=255)
                                                                                                                         taaaaaaTTAATTTTAAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa  <  2:1094531/127‑1 (MQ=255)
                                                                                                                          aaaaaaTTAATTTGAACTTGTTAAAAATTTATTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa  <  2:1002984/128‑1 (MQ=255)
                                                                                                                             |                                                                                                                              
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTA  >  CP016816/165097‑165347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: