Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 20,023 2 bp→TA intergenic (‑744/‑392) JCVSYN2_00065 ← / → JCVSYN2_00070 hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681620,0230AT99.3% 2063.0 / NA 711intergenic (‑744/‑393)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base T (371/333);  total (377/334)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP01681620,0240TA99.6% 2148.4 / NA 711intergenic (‑745/‑392)JCVSYN2_00065/JCVSYN2_00070hypothetical protein/tetracycline resistance ribosomal protection protein Tet(M)
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base A (376/330);  total (377/334)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 726 total aligned reads displayed.

AGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19898‑20145
                                                                                                                             ||                                                                                                                         
    cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      <  2:1117988/128‑1 (MQ=255)
    cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      <  2:1601631/128‑1 (MQ=255)
    cAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTGTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTACTTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtat                                                                                                                      >  2:1332007/1‑128 (MQ=255)
     aGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtata                                                                                                                     <  1:1568244/128‑1 (MQ=255)
     aGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCACACTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTtata                                                                                                                     <  2:1444305/128‑1 (MQ=255)
      ggAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAatttatatatttatat                                                                                                                    <  2:1697145/128‑1 (MQ=255)
       gAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTAtatatttatatt                                                                                                                   >  1:199346/1‑128 (MQ=255)
        aaGAGCAAGATAAAAGGTAGTATTTGTTGGCGCTCCCCCTAGAGTCTTTTACATCTTCGGATAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTa                                                                                                                  >  2:1952030/1‑128 (MQ=255)
        aaGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTa                                                                                                                  >  1:340154/1‑128 (MQ=255)
         agagCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaa                                                                                                                 >  1:1689309/1‑128 (MQ=255)
           agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                               >  2:844555/1‑128 (MQ=255)
           agCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTaaaa                                                                                                               >  2:215083/1‑128 (MQ=255)
             cAAGATAAAAGGTAGTATTTGTTGGCGATGCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTGTTTACTTTCTATTATTAATTTATATATATATATTaaaaaa                                                                                                             >  2:1028428/1‑128 (MQ=255)
                 aTAAAAGGTATGATTTGTTTGCGATTCCCCTATATTCTTTTACATTATAGGAAAAAAAAAACTAATTTTTAATTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTa                                                                                                         <  2:565859/128‑1 (MQ=255)
                  tAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTaa                                                                                                        >  1:1729961/1‑128 (MQ=255)
                    aaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                      >  2:1187493/1‑128 (MQ=255)
                    aaaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaat                                                                                                      >  2:962765/1‑128 (MQ=255)
                     aaGGTAGTATTTGTTTGAGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatt                                                                                                     <  1:1922635/128‑1 (MQ=255)
                     aaGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAATATTAattt                                                                                                     >  2:771389/1‑128 (MQ=255)
                      aGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaatta                                                                                                    >  1:1157708/1‑128 (MQ=255)
                       ggTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattat                                                                                                   <  1:29776/128‑1 (MQ=255)
                         tagtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataa                                                                                                 <  2:1679689/128‑1 (MQ=255)
                           gtaTTTGTTGGCGATGCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               <  1:946693/128‑1 (MQ=255)
                           gtaTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatt                                                                                               >  2:494650/1‑128 (MQ=255)
                            taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                              >  1:1951069/1‑128 (MQ=255)
                            taTTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAaattataatta                                                                                              <  1:1261977/128‑1 (MQ=255)
                              tttGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAattatt                                                                                            >  1:1878053/1‑128 (MQ=255)
                               ttgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTGTATTTTTACTTTGTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAAGTAttt                                                                                           >  1:1629785/1‑128 (MQ=255)
                                tgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGATAACAAAAACTATTTTTTCTTTAATTTCTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAttttt                                                                                         >  2:275208/1‑128 (MQ=255)
                                tgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTAtttt                                                                                          >  2:1081479/1‑128 (MQ=255)
                                tgttgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTACATTAAAAAATTTAAATTATAATTAtttt                                                                                          >  1:1529279/1‑128 (MQ=255)
                                  atgGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTta                                                                                        <  2:579087/127‑1 (MQ=255)
                                      cGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGc                                                                                    >  2:704007/1‑128 (MQ=255)
                                       gATCCCCCTAGAGTCAATTACATCTTCGTAAAACAAAAACTATTATTTCTTTAATTTCTTTTTTTACTTTCAATATATAATTTATATATTTAGATTAAAAAATTTAAATTATAAATATTTTAATAGCa                                                                                   >  1:1005247/1‑128 (MQ=255)
                                         tCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACg                                                                                 >  1:421971/1‑128 (MQ=255)
                                           ccccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               >  1:897160/1‑128 (MQ=255)
                                           ccacTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTg                                                                               <  2:1895021/125‑1 (MQ=255)
                                            cccTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGa                                                                              <  2:67296/128‑1 (MQ=255)
                                              cTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             >  1:622737/1‑127 (MQ=255)
                                              cTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATa                                                                            >  1:73368/1‑128 (MQ=255)
                                              cTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTATTCTTTAATTTATTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATa                                                                            <  2:1239638/128‑1 (MQ=255)
                                                   gTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaa                                                                       >  1:504783/1‑128 (MQ=255)
                                                    tCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaa                                                                      <  2:1818413/128‑1 (MQ=255)
                                                     cTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTaaaa                                                                     <  1:231086/128‑1 (MQ=255)
                                                      ttttACATCTTAGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAg                                                                    <  1:1091547/128‑1 (MQ=255)
                                                          aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTTTAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                >  2:240896/1‑128 (MQ=255)
                                                          aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                <  1:1493847/128‑1 (MQ=255)
                                                          aCATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAg                                                                >  2:1359132/1‑128 (MQ=255)
                                                           cATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGt                                                               >  1:873049/1‑128 (MQ=255)
                                                            aTCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTATTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGAACGTGATAATAAAAAGTTAGTg                                                              >  2:65841/1‑128 (MQ=255)
                                                             tCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGa                                                             >  2:36535/1‑128 (MQ=255)
                                                                  ggAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAg                                                        >  2:1267914/1‑128 (MQ=255)
                                                                    aaaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaa                                                      >  1:271904/1‑128 (MQ=255)
                                                                     aaaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaa                                                     <  1:844555/128‑1 (MQ=255)
                                                                      aaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaaa                                                    >  1:1599744/1‑128 (MQ=255)
                                                                      aaCAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaaa                                                    >  2:1567927/1‑128 (MQ=255)
                                                                        gaaaaaaTAGTTTTTGTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACa                                                  >  1:1199895/2‑128 (MQ=255)
                                                                         aaaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAg                                                 <  1:702068/128‑1 (MQ=255)
                                                                          aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGAt                                                                             >  1:663187/1‑99 (MQ=255)
                                                                          aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                >  2:629580/1‑128 (MQ=255)
                                                                          aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                <  2:1729961/128‑1 (MQ=255)
                                                                          aaaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                >  1:5351/1‑128 (MQ=255)
                                                                           aaaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAGTAAAAGTTAGTGATCAAGAAAACAGTg                                               >  1:381657/1‑128 (MQ=255)
                                                                            atcTATGTTTGCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATCGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGa                                              <  2:802369/126‑1 (MQ=255)
                                                                            aaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAg                                                 >  2:902835/1‑125 (MQ=255)
                                                                            aaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGt                                                >  1:767905/1‑126 (MQ=255)
                                                                            aaCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGa                                              >  2:557159/1‑128 (MQ=255)
                                                                             agtATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAg                                                 <  1:1064386/122‑1 (MQ=255)
                                                                             aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAg                                                 >  2:1064386/1‑124 (MQ=255)
                                                                             aCTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGaa                                             >  1:1424429/1‑128 (MQ=255)
                                                                              cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                            <  1:575489/128‑1 (MQ=255)
                                                                              cTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAg                                            <  2:477024/128‑1 (MQ=255)
                                                                              cTATTTTTTATTTAATTTCTTTTTTTACTTTCTATTTTTATTTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAATACAGTGAAg                                            <  2:1405514/128‑1 (MQ=255)
                                                                               tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                           <  2:531009/128‑1 (MQ=255)
                                                                               tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                           >  1:1393506/1‑128 (MQ=255)
                                                                               tATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGc                                           >  1:603713/1‑128 (MQ=255)
                                                                                 ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  1:1775444/1‑128 (MQ=255)
                                                                                 ttttttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAAATAAAAGTTAGTGAACAAGAAAACAGTGAAGCAc                                         >  2:694102/1‑128 (MQ=255)
                                                                                    tttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAg                                      <  1:289066/128‑1 (MQ=255)
                                                                                    tttCATTAATTTCTATTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAg                                      <  1:1028428/128‑1 (MQ=255)
                                                                                     ttCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGt                                     >  1:988135/1‑128 (MQ=255)
                                                                                       cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGaaa                                                     >  1:1350291/1‑110 (MQ=255)
                                                                                       cTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttt                                   >  2:30375/1‑128 (MQ=255)
                                                                                        tttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACTTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGttta                                  >  2:868301/1‑127 (MQ=255)
                                                                                         ttAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCt                                 <  2:1870936/128‑1 (MQ=255)
                                                                                              ttCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAcc                            <  2:1768415/128‑1 (MQ=255)
                                                                                              ttCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAAcc                            <  2:1642960/128‑1 (MQ=255)
                                                                                                    ttttACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAaaga                      >  1:470372/1‑128 (MQ=255)
                                                                                                         cTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACg                 <  1:794038/128‑1 (MQ=255)
                                                                                                          tttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGa                >  1:1821634/1‑128 (MQ=255)
                                                                                                           ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa               <  2:457284/128‑1 (MQ=255)
                                                                                                           ttCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGAACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaa               <  1:553126/128‑1 (MQ=255)
                                                                                                            tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa              <  1:1862312/128‑1 (MQ=255)
                                                                                                            tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa              >  2:410785/1‑128 (MQ=255)
                                                                                                            tCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa              >  2:1849376/1‑128 (MQ=255)
                                                                                                            tCTAATTTTAATTTTTAAATTTAAATTAAAAAATTTAAATATTTATTATTTTTATATAACGTGATAATTAAAAGGTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaa              <  1:1297632/128‑1 (MQ=255)
                                                                                                             cTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaa             >  2:1518244/1‑128 (MQ=255)
                                                                                                               aTTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaaa           <  2:743780/128‑1 (MQ=255)
                                                                                                               aTTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGaaaaaa           >  1:1118289/1‑128 (MQ=255)
                                                                                                                      atttatatatttatatTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACaaaaaa    >  1:1674018/1‑128 (MQ=255)
                                                                                                                             ||                                                                                                                         
AGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCCCCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATAATTTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATAATTAAAAGTTAGTGAACAAGAAAACAGTGAAGCACCAGTTTCTGAACCAAAAGAAGACGAAAAAACAAAAAAAG  >  CP016816/19898‑20145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: