Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 484,791 T→A intergenic (‑100/+71) JCVSYN2_02220 ← / ← JCVSYN2_02225 hypothetical protein/50S ribosomal protein L7/L12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816484,7910TA98.0% 9588.3 / 32.6 3219intergenic (‑100/+71)JCVSYN2_02220/JCVSYN2_02225hypothetical protein/50S ribosomal protein L7/L12
Reads supporting (aligned to +/- strand):  ref base T (29/35);  new base A (1535/1615);  total (1565/1654)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3322 total aligned reads displayed.

TATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484664‑484913
                                                                                                                               |                                                                                                                            
       tAAAAAATCGTCAAACCTCATAGAATAAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTaatataa                                                                                                                       <  2:2003218/128‑6 (MQ=255)
        aaataaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaa                                                                                                                     <  1:3297829/128‑1 (MQ=255)
         aaaaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaa                                                                                                                    >  2:3807856/1‑128 (MQ=255)
           aaaTAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAatataaaaagc                                                                                                                  >  2:1855008/1‑126 (MQ=255)
              tAGTCAAAACTCATAGATTTAATCCTTTTATATTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAAAATAAAAAGCAAt                                                                                                               >  2:4272619/1‑128 (MQ=255)
              tAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAAt                                                                                                               <  2:5212319/128‑1 (MQ=255)
                 tCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTaa                                                                                                            >  2:1062682/1‑128 (MQ=255)
                  cAAAACTCATCCAATTAAACCTTTTATAGATGACCCAAAAATAAGCTAGAGCAAAGCTGAAGCTCATAACAACTAAACTACACTCGATAAATTAATAATAATTTTTTTTAATATAAAAAGCAATTaaa                                                                                                           <  2:6482073/128‑1 (MQ=255)
                    aaaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGACGCTCTTAACAACTAAACTACACTCGATTATTTATTTATATTTTTTTTAATATAAAAAGCAATTatccac                                                                                                        >  2:4860841/1‑123 (MQ=255)
                     aaCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacac                                                                                                        <  1:5347731/128‑1 (MQ=255)
                      aCTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacaca                                                                                                       >  1:3039117/1‑128 (MQ=255)
                       cTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAacccac                                                                                                      >  2:5887766/1‑128 (MQ=255)
                          aTAGATTTAATCCTTTTATAGTTGACCAAAAAAAAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGAATATTTAATTATATTATTTTTTAATATAAAAATCAATTAAACACACaaa                                                                                                   >  2:4392002/1‑128 (MQ=255)
                               tttAATACATTTATTTTTGACCAAAAAATTATAATTTTGATATCATTATCTCTTAACAACTTAACTACACTCGATTAATTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                              <  1:849615/128‑1 (MQ=255)
                                ttAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCa                                                                                             <  1:5435790/128‑1 (MQ=255)
                                 tAATCCTTTTATAGTTGACCAAAAAATAATATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTAGATTATATAATTAAATTTTTTTTTTAATATAAAAAGCTATTAAAAACACaaaaaagaa                                                                                             >  2:4970657/1‑124 (MQ=255)
                                 tAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGAATATTTAATTATATATTTTTTTAATAAAAAAAGAAATAAAACAAACAAAAACTCAc                                                                                            >  1:6686755/1‑128 (MQ=255)
                                 tAATCCTTTTATAGTTGAACAAAAAATAAGATAGAGCATAGCAGAAGCTATTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAc                                                                                            <  2:266650/128‑1 (MQ=255)
                                     ccTTTTATAGTTGACCAAAATATAAGATTGAGCATATCAGAAGCTCTTAACAACTATTCTACACTAGTTTATTTAATTATATTTTTTTTTAATATAAAAATCAATTAAACACACAAAAACTCAccata                                                                                        <  2:2555024/128‑4 (MQ=255)
                                     ccTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccata                                                                                        <  1:1183672/128‑4 (MQ=255)
                                       ttttATAGTAGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                      <  1:5244556/128‑1 (MQ=255)
                                         ttATAGTTGACCAAAAAATCAGTTAGAGCATAGCAGAAGCTCTTTACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATg                                                                                   <  2:4624662/128‑1 (MQ=255)
                                          tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                  >  1:2272931/1‑128 (MQ=255)
                                          tataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATgg                                                                                  <  1:5131720/128‑1 (MQ=255)
                                           ataGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCAccataatgggt                                                                                  <  1:5980379/128‑10 (MQ=255)
                                           ataGTTGAACAAACAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGt                                                                                 <  2:4019152/128‑1 (MQ=255)
                                             aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTATTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGa                                                                               >  1:5859813/1‑128 (MQ=255)
                                             aGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTc                                                                                              >  2:1679479/1‑114 (MQ=255)
                                              gTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAg                                                                              >  2:6228762/1‑128 (MQ=255)
                                                  aCCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTa                                                                          <  2:5708215/128‑1 (MQ=255)
                                                   ccAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaa                                                                         >  1:2544580/1‑128 (MQ=255)
                                                      aaaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaaaa                                                                      <  2:1181425/128‑1 (MQ=255)
                                                       aaaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCATAATGGGTGAGtttttgtgt                                                                     <  2:5315771/128‑7 (MQ=255)
                                                        aaaTAAGATAGAGCATAGCAGAATCTCTAAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  1:962114/128‑1 (MQ=255)
                                                        aaaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAatt                                                                    <  1:5035903/128‑1 (MQ=255)
                                                         aaTAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAac                                                                                                          <  2:1542712/90‑1 (MQ=255)
                                                            aaGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTc                                                                <  1:2824409/128‑1 (MQ=255)
                                                             aGATAGAGCATAGCAGAAGCGCTTAAATATTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCa                                                               <  2:4512241/128‑1 (MQ=255)
                                                                tAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaa                                                            <  1:1284362/128‑1 (MQ=255)
                                                                 agagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCaaaaa                                                           >  1:1513427/1‑128 (MQ=255)
                                                                  gagCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          <  1:1002607/128‑1 (MQ=255)
                                                                  gaccATACCAGAAGCTCTTAACAAATAAACTACACTCGATTATATAATTATATTTATTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAt                                                          <  2:1431318/125‑1 (MQ=255)
                                                                   agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         >  1:4660626/1‑128 (MQ=255)
                                                                   agCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAtt                                                         >  2:3654572/1‑128 (MQ=255)
                                                                    gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTaaa                                                                        <  1:6472789/112‑1 (MQ=255)
                                                                    gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                        >  1:4067270/1‑128 (MQ=255)
                                                                    gCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                        >  1:5508177/1‑128 (MQ=255)
                                                                      aTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                      <  2:4140546/128‑1 (MQ=255)
                                                                      aTAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGa                                                      <  2:5473695/128‑1 (MQ=255)
                                                                       tatcAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  1:2954836/125‑1 (MQ=255)
                                                                       aaGCAGTAGCTCTTAACAACTAAACTAAACTCGCTTAATGAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGat                                                     <  2:3517579/127‑1 (MQ=255)
                                                                            gAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                >  1:6780196/1‑128 (MQ=255)
                                                                            gAAGCTCCTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAttt                                                <  1:3957417/128‑1 (MQ=255)
                                                                               gCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                             >  2:3011046/1‑128 (MQ=255)
                                                                               gCTCTTAACAACTAAACTACACTAGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaa                                             <  2:754814/128‑1 (MQ=255)
                                                                                ctctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                            >  1:3699852/1‑128 (MQ=255)
                                                                                  ttttactactatacttCAATCTATTATTTTATTTTATTTTTTTTTTATATAAAAAGCAATTAAACACACATAATCTCACCCATTATGGTGATTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                          <  2:1283321/121‑1 (MQ=255)
                                                                                  ctaaaccacTAAACTACCCACGATTAGATAAATATATATTTATATAATAAAAAAAGCAATTAAACACACACAAACTCTCCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTAATTTAAATc                                          <  2:3372838/125‑1 (MQ=255)
                                                                                  ctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAAttt                                                        >  1:6310529/1‑114 (MQ=255)
                                                                                  ctTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                          <  1:2040815/128‑1 (MQ=255)
                                                                                  ctTAACAACTAAACTAAACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATc                                          <  2:3246312/128‑1 (MQ=255)
                                                                                   ttAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCa                                         <  1:1622213/128‑1 (MQ=255)
                                                                                    tAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCTAAAATTTGATTATTTTAAATCaa                                        >  1:1776541/1‑128 (MQ=255)
                                                                                      acaacTAAACTACACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                      >  1:5742519/1‑128 (MQ=255)
                                                                                      acaacTAAACTACACTCGATTATTTAATTATATTTTTTATTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                      <  1:3127739/128‑1 (MQ=255)
                                                                                      acaacTAAAATACAATACATTAATTTATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAtt                                      <  1:1916378/128‑1 (MQ=255)
                                                                                        aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTa                                                                                      >  2:2421474/1‑79 (MQ=255)
                                                                                        aactaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    <  1:5635361/128‑1 (MQ=255)
                                                                                        aacaaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGa                                    <  2:5080388/128‑1 (MQ=255)
                                                                                         actaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   <  1:2429696/128‑1 (MQ=255)
                                                                                          ctaaactaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATTTTTAGTTTAAAAATAATTCAAAAATATGAATATTTTAAATCAAATGAAg                                  >  1:4791120/1‑128 (MQ=255)
                                                                                           taaactaCACACGATTAATTAAATATAATTTTTTTTAATATAAAAAGCAATAAAACACACAAAAACTCACCAATTATCGTTAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGc                                 >  2:5570719/1‑128 (MQ=255)
                                                                                               ctaCACTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAAt                                       <  1:3843577/118‑1 (MQ=255)
                                                                                                 acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGaa                                   >  2:6123640/1‑120 (MQ=255)
                                                                                                 acacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGc                           <  2:2782769/128‑1 (MQ=255)
                                                                                                  cacTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGAAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCt                          <  1:2665779/128‑1 (MQ=255)
                                                                                                  cacTCGATTATTTAATTATATTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                         <  2:4711549/128‑1 (MQ=255)
                                                                                                   acTCGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCtt                         >  1:1394046/1‑128 (MQ=255)
                                                                                                      cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                      >  1:4221592/1‑128 (MQ=255)
                                                                                                      cGATTATTTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCaa                      >  2:6772670/1‑128 (MQ=255)
                                                                                                        attatcTACATATATTTTTTTTTAAAATAAATAGCAATTAAACACACAAAAACTCACCCAATATTGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGATCCAGCTTCAACt                    <  1:2160253/128‑1 (MQ=255)
                                                                                                         ttattTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTaaa                                            >  1:3420858/1‑103 (MQ=255)
                                                                                                           attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAAt                 <  1:4307878/128‑1 (MQ=255)
                                                                                                           attTAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATAATTTTAAATCAATTGAATCTCCAGCTTCAACTAAt                 >  1:3561258/1‑128 (MQ=255)
                                                                                                              tAATTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTa              >  2:3102109/1‑128 (MQ=255)
                                                                                                               aaTTATATTTTTTTTTAATATATAAAGCAATTAAACACACTAAAACTCACCCATTATGGTGAGTTTAATAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg             <  1:607230/128‑1 (MQ=255)
                                                                                                               aaTTATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAg             <  2:2137869/128‑1 (MQ=255)
                                                                                                                 ttATATTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCt           >  2:871283/1‑128 (MQ=255)
                                                                                                                  tataTTTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGATTAAAAATTATTCAAAAATTTGATTATTTTAATTCAATTAAAGCTCCAGATTCAAGTCATTTAGCt           >  2:2936593/1‑128 (MQ=255)
                                                                                                                   atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAATAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  1:713077/1‑128 (MQ=255)
                                                                                                                   atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCttt         >  1:4426539/1‑128 (MQ=255)
                                                                                                                   atatTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTACAGCTTCAACTAATTTAGCttt         >  2:6014536/1‑128 (MQ=255)
                                                                                                                     atTTTTTTTTAATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCa       >  2:2292642/1‑128 (MQ=255)
                                                                                                                      tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt      <  1:6229565/128‑1 (MQ=255)
                                                                                                                      tttttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAt      >  1:4575340/1‑128 (MQ=255)
                                                                                                                        tttttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCAttt    >  2:6642271/1‑128 (MQ=255)
                                                                                                                          tttttaATATAAAAAGCAATTAAACACATAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct  <  2:3924558/128‑1 (MQ=255)
                                                                                                                          tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct  >  1:6589190/1‑128 (MQ=255)
                                                                                                                          tttttaATATAAAAAGCAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATttct  <  2:6353230/128‑1 (MQ=255)
                                                                                                                          tttttaATATAAAAAGCAATTAAACACACAAAAACTCACACATTATGGTGAGTTTAAAAATTATTCAAAAAATTGATTATTTTAAATCAATTGAAGATCAAGCTTCAACTAATTAAGCTTTAATttct  >  1:4939630/1‑128 (MQ=255)
                                                                                                                               |                                                                                                                            
TATTATTTAAAAAATAGTCAAAACTCATAGATTTAATCCTTTTATAGTTGACCAAAAAATAAGATAGAGCATAGCAGAAGCTCTTAACAACTAAACTACACTCGATTATTTAATTATATTTTTTTTTTAAAAAAAAAACAATTAAACACACAAAAACTCACCCATTATGGTGAGTTTAAAAATTATTCAAAAATTTGATTATTTTAAATCAATTGAAGCTCCAGCTTCAACTAATTTAGCTTTCATTTCT  >  CP016816/484664‑484913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: