BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	32,707	A→T	I1067F (ATT→TTT)	JCVSYN2_00105 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	32,707	0	A	T	99.3%	9168.3 / NA	2905	I1067F (ATT→TTT)	JCVSYN2_00105	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (16/5); new base T (1470/1409); total (1488/1417)
Fisher's exact test for biased strand distribution p-value = 2.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3011 total aligned reads displayed.

TGTTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGA  >  CP016816/32580‑32831

  ttACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCAtttttt                                                                                                                            <  1:1233644/128‑1 (MQ=255)

   tACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATttttta                                                                                                                           <  2:4484451/128‑1 (MQ=255)

     cTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTaaa                                                                                                                         >  2:4103729/1‑128 (MQ=255)

        aaCCTTTAACTGAGATTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCt                                                                                                                      <  2:1980495/128‑1 (MQ=255)

         aCCTTTACCTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCtt                                                                                                                     <  2:1179777/128‑1 (MQ=255)

           cTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCTTTTTTTAAATCTTAt                                                                                                                   >  2:1720131/1‑128 (MQ=255)

             ttAACTCAGTTTGTTGGAGAACTTTTAAAAAATATTTTTAAAGATAAAGTGCAAACTACTGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTAttt                                                                                                                 <  1:6618846/128‑1 (MQ=255)

              tAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTa                                                                                                                <  2:757445/128‑1 (MQ=255)

                   cAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAg                                                                                                           <  2:6359323/128‑1 (MQ=255)

                   cAGATTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGCTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAg                                                                                                           <  2:5074772/128‑1 (MQ=255)

                    aGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACGACAGGTGATTGAAAAGAAAAGATCTTAAACTTATTTTTAGGTTATTCTCCTTACTTTTTTAAATCTTAGTTAACAAGt                                                                                                          >  2:3548582/1‑128 (MQ=255)

                     gTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTg                                                                                                         >  1:4419740/1‑128 (MQ=255)

                          ttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAATGATCTTAAACTTAATATTAGGTTATTCTCCTTCATTTTTTATATCTTATTTAAAAAGTGAAtct                                                                                                    >  1:4192149/1‑128 (MQ=255)

                          ttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtct                                                                                                    >  2:4984187/1‑128 (MQ=255)

                          ttgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtct                                                                                                    >  1:247559/1‑128 (MQ=255)

                           tgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtctc                                                                                                   >  1:3760590/1‑128 (MQ=255)

                           tgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAAtctc                                                                                                   >  2:6809165/1‑128 (MQ=255)

                           tgGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTATTTAAATCTTATTTAACAAGTGAAtctc                                                                                                   >  1:6395050/1‑128 (MQ=255)

                             gagaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                 <  2:275238/128‑1 (MQ=255)

                             gagaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                 <  2:5344965/128‑1 (MQ=255)

                             gagaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                 <  1:1494735/128‑1 (MQ=255)

                             gagaACTTTAAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGAGCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGa                                                                                                 <  2:340206/128‑1 (MQ=255)

                              agaACTTTTAAATACTATTTTTTAAGATAAAGTTCAAACTACAGGTGAGTGAAAAGAAAATATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGaa                                                                                                <  2:424656/128‑1 (MQ=255)

                              agaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGaa                                                                                                <  1:3101731/128‑1 (MQ=255)

                              agaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTACTTCATTTTTTAAATCTTATTTAACAAGTGAATATCGaa                                                                                                >  1:3215723/1‑128 (MQ=255)

                               gaACTTTTAATAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                               <  2:148247/128‑1 (MQ=255)

                               gaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                               <  2:2130824/128‑1 (MQ=255)

                               gaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                               >  1:5051817/1‑128 (MQ=255)

                               gaACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGGGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAag                                                                                               <  2:3181621/128‑1 (MQ=255)

                                 aCTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAagag                                                                                             <  1:5381024/128‑1 (MQ=255)

                                 aCTTTTAAAAACTATTTGTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAATATATTAAACTTAATTTTAGGGTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAagag                                                                                             <  2:2594163/128‑1 (MQ=255)

                                  cTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCCAAGAGc                                                                                            <  2:4562713/128‑1 (MQ=255)

                                   ttttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                           >  1:4624464/1‑128 (MQ=255)

                                   ttttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCt                                                                                           <  1:5171425/128‑1 (MQ=255)

                                    tttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGATAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTGTTTAAATCTTAATTAACAACTGAAACTCGAAGAtctc                                                                                          >  2:2261375/1‑128 (MQ=255)

                                     ttAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCa                                                                                         >  2:6230761/1‑128 (MQ=255)

                                       aaaaaCTATTTTTAAAGATAAAGTTCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAt                                                                                       <  1:3987140/128‑1 (MQ=255)

                                       aaaaaCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGATATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAt                                                                                       <  2:2479959/128‑1 (MQ=255)

                                        aaaaCTATTTTTAAAGATAAACTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAtt                                                                                      <  2:665815/128‑1 (MQ=255)

                                         aaaCTATTTTTAAAGATAAAGTGCAAACTACAGCTGAGTGAAAAGAAAAGATCTTAAACTTAATTTAAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAAttt                                                                                     <  2:8545/128‑1 (MQ=255)

                                           aCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAaga                                                                                              >  1:4342782/1‑117 (MQ=255)

                                           aCTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTc                                                                                   >  1:4920122/1‑128 (MQ=255)

                                            cTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTa                                                                                                                <  2:5980270/98‑1 (MQ=255)

                                             tATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCtt                                                                                 <  1:2081454/128‑1 (MQ=255)

                                              aTTTTTAAAGATAAAGTGCAAACTAAAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCttt                                                                                <  1:1004649/128‑1 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             >  1:1124020/1‑128 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             <  1:296485/128‑1 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTgg                                                                             >  1:1682369/1‑128 (MQ=255)

                                                 tttAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTACTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGATGATCTCAATTTTCTTTTgg                                                                             >  1:1849083/1‑128 (MQ=255)

                                                      aGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGAc                                                                        >  2:1581915/1‑128 (MQ=255)

                                                        aTAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGActa                                                                      >  1:2587890/1‑126 (MQ=255)

                                                          taaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAAATTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaa                                                                    <  2:5597013/127‑1 (MQ=255)

                                                          caaGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACaaaa                                                                    <  1:5957509/127‑1 (MQ=255)

                                                            aGTGCAAACTACAGGTCCGTGAAAAGAAAAGAGCGTAAACTTCATTTTAGGTTCTTCTCCTTCATTTTTTACATCTTAGTAAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTGGACAAaaac                                                                  <  2:2858699/128‑1 (MQ=255)

                                                                 aaaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACt                                                             <  1:1948196/128‑1 (MQ=255)

                                                                 aaaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACt                                                             <  2:4662194/128‑1 (MQ=255)

                                                                  aaCTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACta                                                            >  2:3306162/1‑128 (MQ=255)

                                                                    cTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACtata                                                          >  2:4841220/1‑128 (MQ=255)

                                                                       cAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAAtt                                                       >  1:6520124/1‑128 (MQ=255)

                                                                        aGGTGAGTGAAAAGAAAAGATATTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATATTATTTAACAAGTGAATCTCGAAGAGGTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTc                                                      <  1:3509508/128‑1 (MQ=255)

                                                                            gagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGa                                                                         <  2:1296166/105‑1 (MQ=255)

                                                                            gagtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCcaca                                                  >  1:4508045/1‑128 (MQ=255)

                                                                             agtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaa                                                 >  1:5282310/1‑128 (MQ=255)

                                                                              gtgaAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACaaa                                                >  1:1359394/1‑128 (MQ=255)

                                                                                   aagaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCTCAAAAAGAt                                           >  2:1840012/1‑128 (MQ=255)

                                                                                   aagaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGAt                                           <  2:6511978/128‑1 (MQ=255)

                                                                                    agaaacgaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATc                                          <  2:365567/128‑1 (MQ=255)

                                                                                     gaaaagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCa                                         >  2:5206749/1‑128 (MQ=255)

                                                                                        aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                      >  1:3656336/1‑128 (MQ=255)

                                                                                        aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                      >  1:60573/1‑128 (MQ=255)

                                                                                        aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                      >  1:28389/1‑128 (MQ=255)

                                                                                        aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                      >  2:6101220/1‑128 (MQ=255)

                                                                                        aagaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAtt                                      >  1:4762837/1‑128 (MQ=255)

                                                                                         agaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTa                                     >  1:920874/1‑128 (MQ=255)

                                                                                          gaTCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAg                                    <  1:3354477/128‑1 (MQ=255)

                                                                                             cTTAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCaa                                 >  2:1041005/1‑128 (MQ=255)

                                                                                              ttAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAATCAAAATATAATTCCACAAAAAGATCAATTAGCAAc                                >  1:5505403/1‑128 (MQ=255)

                                                                                               tAAACTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACt                               >  1:811200/1‑128 (MQ=255)

                                                                                                  aCTTAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAtt                            <  1:2211692/128‑1 (MQ=255)

                                                                                                     tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCATAAAAAGATCAATTAGCAACTAttttt                         <  1:5866216/128‑1 (MQ=255)

                                                                                                     tAATTTTAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                         <  1:2715501/128‑1 (MQ=255)

                                                                                                     tAAGTTTAGGTTATTCTTCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTAttttt                         <  2:4366089/128‑1 (MQ=255)

                                                                                                      taTTTTAGGTTATTCTACTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTa                        <  1:6845236/127‑1 (MQ=255)

                                                                                                        ttttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTaaa                      <  1:5729491/128‑1 (MQ=255)

                                                                                                         tttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAc                     <  2:2393416/128‑1 (MQ=255)

                                                                                                          ttAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAAcc                    <  1:615369/128‑1 (MQ=255)

                                                                                                           tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTTAATCTCTAAGAGATCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTACACAAAAAGATCAATTAGCAACTATTTTTAAACCt                   <  1:4092873/128‑1 (MQ=255)

                                                                                                           tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCTACTATTTTTAAACCt                   >  1:2338183/1‑128 (MQ=255)

                                                                                                           tAGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCt                   >  1:6723748/1‑128 (MQ=255)

                                                                                                            aGGTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTa                  <  2:5458880/128‑1 (MQ=255)

                                                                                                             ggTTATTCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCcac                                                   >  1:5622080/1‑94 (MQ=255)

                                                                                                                  ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCaa            >  2:3773025/1‑128 (MQ=255)

                                                                                                                  ttCTCCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTATTAAACCTAAATCaa            >  2:4004424/1‑128 (MQ=255)

                                                                                                                    ttgcTTCATTTTTTAATTCTTATTTATCAAGTGAATGTGGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCTATTAGCAACTATTTTTAAACCTAAATCAAAt          <  2:2984858/125‑1 (MQ=255)

                                                                                                                    ctcCTTCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAAt          >  2:3894815/1‑128 (MQ=255)

                                                                                                                    ctcCTTCATATTTTAAATCTTATTTAACAAGTGACTCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGAGCAATTAGCAACTATTTTTAAACCTAAATCAAAt          <  2:5871511/128‑1 (MQ=255)

                                                                                                                        ttCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAAATAGCAACAATTTATAAACCTAAATCAAATAACa      >  1:627896/1‑128 (MQ=255)

                                                                                                                         tCATTTTTTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACAt     >  2:5756734/1‑128 (MQ=255)

                                                                                                                            ttttttAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGa  >  1:3886178/1‑128 (MQ=255)

                                                                                                                            ttttttAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGAAAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGa  >  2:3067329/1‑128 (MQ=255)

TGTTACTCAACCTTTAACTCAGTTTGTTGGAGAACTTTTAAAAACTATTTTTAAAGATAAAGTGCAAACTACAGGTGAGTGAAAAGAAAAGATCTTAAACTTAATTTTAGGTTATTCTCCTTCATTTATTAAATCTTATTTAACAAGTGAATCTCGAAGAGCTCAATTTTCTTTTGGTTGACAAAAACAAACTATAATTCCACAAAAAGATCAATTAGCAACTATTTTTAAACCTAAATCAAATAACATTGA  >  CP016816/32580‑32831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`