Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 153,394 G→A S79F (TCT→TTT)  JCVSYN2_00620 ← transcription elongation factor GreA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816153,3940GA99.4% 9309.5 / NA 3023S79F (TCT→TTT) JCVSYN2_00620transcription elongation factor GreA
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base A (1523/1470);  minor base T (8/11);  total (1535/1488)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3051 total aligned reads displayed.

TTAGATTAGAAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAGAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACTTAAATCTCCTTGATTACG  >  CP016816/153269‑153518
                                                                                                                             |                                                                                                                            
 tagattagaAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAat                                                                                                                           <  1:6301841/128‑1 (MQ=255)
  agattagaAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAatc                                                                                                                          <  2:3552397/128‑1 (MQ=255)
      tagaAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAAtcatca                                                                                                                      <  1:2404634/128‑1 (MQ=255)
      tagaAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAAtcatca                                                                                                                      >  2:2822935/1‑128 (MQ=255)
       agaAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCaa                                                                                                                     >  1:617697/1‑128 (MQ=255)
       agaAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCaa                                                                                                                     >  1:804647/1‑128 (MQ=255)
       agaAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCaa                                                                                                                     >  1:3859731/1‑128 (MQ=255)
        gaAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAAt                                                                                                                    <  1:3245916/128‑1 (MQ=255)
        gaAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAAt                                                                                                                    >  1:4508627/1‑128 (MQ=255)
          aaaaGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATaa                                                                                                                  >  2:1800968/1‑128 (MQ=255)
               gATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTa                                                                                                             >  1:5792434/1‑128 (MQ=255)
               gATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTa                                                                                                             <  2:2310164/128‑1 (MQ=255)
                  cAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAg                                                                                                            >  1:4763760/1‑126 (MQ=255)
                   aGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCTATAACTTTAGctc                                                                                                         >  1:6656183/1‑128 (MQ=255)
                    gCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCACTAACTTTAGctct                                                                                                        >  1:2143417/1‑128 (MQ=255)
                    gCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGctct                                                                                                        >  1:3020782/1‑128 (MQ=255)
                     cTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTa                                                                                                       <  2:6758990/128‑1 (MQ=255)
                     cTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTa                                                                                                       <  1:651891/128‑1 (MQ=255)
                       tCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTatt                                                                                                     <  1:46270/128‑1 (MQ=255)
                           tAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTattaatt                                                                                                 <  2:446237/128‑1 (MQ=255)
                             gCACCATCAATTATAATTTCATTTAATTTATTATCTTAACTTGTAACAAATTGTACAGTTGATCCAATTTTAACTTTACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTAttaattaa                                                                                               <  1:4166831/128‑1 (MQ=255)
                              cACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTAttaattaa                                                                                               <  2:4567014/127‑1 (MQ=255)
                              cACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTaaa                                                                                              >  2:60091/1‑128 (MQ=255)
                               aCCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAg                                                                                             >  1:2877244/1‑128 (MQ=255)
                               aCCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAg                                                                                             >  1:2722821/1‑128 (MQ=255)
                                aaactattttaAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGt                                                                                            <  1:3607013/121‑1 (MQ=255)
                                  aacaaTTTTAATTTCTTTTAATTTATTCTCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGttt                                                                                          <  2:5409778/128‑1 (MQ=255)
                                  aacaaTTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGttt                                                                                          >  2:3416055/1‑128 (MQ=255)
                                   acaaTTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTTAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTc                                                                                         <  2:1129199/128‑1 (MQ=255)
                                   acaaTTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTc                                                                                         >  2:4822584/1‑128 (MQ=255)
                                    caaTTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCa                                                                                        >  2:1927523/1‑128 (MQ=255)
                                     aaTTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCaa                                                                                       <  1:4389607/128‑1 (MQ=255)
                                     aaTTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCaa                                                                                       <  2:3852091/128‑1 (MQ=255)
                                      aagttAATTTCTTTTAATTTATTATCTAAACTTGATACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAATTCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAAc                                                                                      <  2:4688437/125‑1 (MQ=255)
                                        tttAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAActt                                                                                    <  1:3497384/128‑1 (MQ=255)
                                        tttAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAActt                                                                                    <  1:5896668/128‑1 (MQ=255)
                                         ttAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCACcttc                                                                                   >  2:489459/1‑128 (MQ=255)
                                           aaTTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCATcttctt                                                                                 >  1:5527712/1‑128 (MQ=255)
                                           aaTTTATTTTAATTTATTATCTAAACTTGAAACAAATTGTAGATTTGATCAAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAActtctt                                                                                 <  2:1337324/128‑1 (MQ=255)
                                            aatttttttAATTTATTATCTAAACTAGAATCAAATAGTACATTTGATAAAATTTTAACTTCACCATTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCttt                                                                                <  1:1522804/123‑1 (MQ=255)
                                             tttCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTa                                                                               >  1:2047317/1‑128 (MQ=255)
                                             ttggttttAATTTATTATGTATTGTTGAATCAAATTGTACAGTTGTTCGATTTTTAACTTCACCAGTTGAATGAGTGTTAAAATCATCAATAACTTTAGCTCTAATAATTAAAGTTGCAACTTCTTTa                                                                               <  2:3003862/124‑1 (MQ=255)
                                                cTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAActtct                                                                                  <  1:1134913/122‑1 (MQ=255)
                                                cTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAAtt                                                                            <  2:5743062/128‑1 (MQ=255)
                                                 ttttAATTTTTTATCTAAACTTTAAACAAATTGTAAAGTTGTTCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTc                                                                           <  2:2576817/128‑1 (MQ=255)
                                                 ttttAATTTCTTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAActtc                                                                                   <  1:4263725/120‑1 (MQ=255)
                                                 ttttAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTCGCTCTATTAATTAAAGTTTCAACTTCTTTAATTc                                                                           <  1:1920066/128‑1 (MQ=255)
                                                  tttAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCt                                                                          >  2:396701/1‑128 (MQ=255)
                                                    tAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTg                                                                        >  1:1271400/1‑128 (MQ=255)
                                                     aaTTTCTTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGc                                                                       <  2:792764/128‑1 (MQ=255)
                                                       tttATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTACTAATTAAAGTTTCAACTTCTTTAATTCTTGCtt                                                                     <  2:665039/128‑1 (MQ=255)
                                                        ttattaTCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTc                                                                    <  2:4115207/128‑1 (MQ=255)
                                                           ttatCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTGTATTAATTAAAGTTTCAACGTCGTTAATTCTTGCTTCAAc                                                                 >  2:4237072/1‑128 (MQ=255)
                                                           ttatCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAAc                                                                 >  2:327765/1‑128 (MQ=255)
                                                            tatCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACt                                                                <  1:4982836/128‑1 (MQ=255)
                                                                   aCTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCtt                                                         >  2:5141196/1‑128 (MQ=255)
                                                                   aCTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCtt                                                         >  2:6297617/1‑128 (MQ=255)
                                                                    cTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTg                                                        <  1:718615/128‑1 (MQ=255)
                                                                    cTTGAAACAAATTGTACAGTTCCTCCAATTTTAACTTCCCCAGTTGAATGAGTTTTAAAATCATCAATAACTGTAGCTCTATTAATTACAGTTTCAACTTCGTTAATTCTTGCGTCCACTTCAGcggg                                                        >  2:6544629/1‑125 (MQ=255)
                                                                       gAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGTTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTACAGCTTGTCt                                                     >  2:528137/1‑128 (MQ=255)
                                                                         aaCAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAGTAACTTTa                                                                                                             >  2:3116759/1‑70 (MQ=255)
                                                                            aaaTTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTc                                                >  1:5136163/1‑128 (MQ=255)
                                                                              aTTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTa                                                                               <  2:3757658/95‑1 (MQ=255)
                                                                              aTTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTa                                              >  2:2458832/1‑128 (MQ=255)
                                                                               ttGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTag                                             >  1:5994070/1‑128 (MQ=255)
                                                                               ttGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTag                                             >  1:3372130/1‑128 (MQ=255)
                                                                               ttGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTag                                             >  1:4006665/1‑128 (MQ=255)
                                                                               ttGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTa                                              <  2:6183550/127‑1 (MQ=255)
                                                                                  tACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCATCTTGTCTGTTTCTagcag                                          >  2:887110/1‑128 (MQ=255)
                                                                                   aCAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTagcagc                                         >  1:3141393/1‑128 (MQ=255)
                                                                                      gTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGcatc                                      >  1:4884304/1‑128 (MQ=255)
                                                                                        tGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGcatcat                                    <  1:1803158/128‑1 (MQ=255)
                                                                                        tGATCCAATTTTAACTTCAACAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGcatcat                                    <  2:587237/128‑1 (MQ=255)
                                                                                        tGATCAAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGcatcat                                    <  2:2064682/128‑1 (MQ=255)
                                                                                          aTCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATaa                                  <  2:5538652/128‑1 (MQ=255)
                                                                                          aTCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATaa                                  <  1:1656761/128‑1 (MQ=255)
                                                                                          aTCCAATTTTAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTATCAACTTCTTTAATTCTTGCTTCAACTTCATCTAGTCTGTATCTAGCAGCATCATaa                                  >  1:6407626/1‑128 (MQ=255)
                                                                                             cAATTTTAACTTCCCCAGTAGAATGAGTGTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCa                               <  1:3729192/128‑1 (MQ=255)
                                                                                                  ttAACTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCAtt                          <  2:3653416/128‑1 (MQ=255)
                                                                                                  ttAACTTCACCAGTTGAATGAGAGTTAAAATCATAAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCAtt                          <  2:1454651/128‑1 (MQ=255)
                                                                                                    aaCTTCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCAtttt                        >  1:6781019/1‑128 (MQ=255)
                                                                                                    aaCTTCACCAGATGCATTAGATTAAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTGCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCAtttt                        <  2:700431/128‑1 (MQ=255)
                                                                                                     aCTTCACCAGTTCAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTTATTAAAGATTCCACATCTTTAATTCTTGCTTCACCTTCCGCTAGTCTGTTTCTAGCAGCCTCATTAACAGcctggtc                       >  2:5014747/1‑122 (MQ=255)
                                                                                                       ttCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCAtt                          <  2:1589809/123‑1 (MQ=255)
                                                                                                        tCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCaaa                    >  2:1024033/1‑126 (MQ=255)
                                                                                                        tCACCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACt                    <  2:6425403/128‑1 (MQ=255)
                                                                                                         cACCAGTTGAATGAGATTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACtt                   <  1:5275191/128‑1 (MQ=255)
                                                                                                          aCCAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCAGAATCCGCATTTTCACTTa                  >  2:5641665/1‑128 (MQ=255)
                                                                                                           ccAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTaa                                                                              <  2:4327840/67‑1 (MQ=255)
                                                                                                           ccAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACTTaa                 <  2:1684491/128‑1 (MQ=255)
                                                                                                           ccAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATGTTCACTTaa                 >  1:2563555/1‑128 (MQ=255)
                                                                                                            cAGTTGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGAATTTTCACTTaaa                >  2:2199278/1‑128 (MQ=255)
                                                                                                               ttGAATGAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACTTAAAtct             <  1:5675458/128‑1 (MQ=255)
                                                                                                                     gagaTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACTTAAATCTCCTTGa       <  1:5408255/128‑1 (MQ=255)
                                                                                                                     gAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACTTAAATCTCCTTGa       >  1:2257214/1‑128 (MQ=255)
                                                                                                                     gAGTTTTAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTATTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCCCTTAAATCTCCTTGa       >  1:1388770/1‑128 (MQ=255)
                                                                                                                        ttttAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACTTAAATCTCCTTGATTa    >  2:1228293/1‑128 (MQ=255)
                                                                                                                        ttttAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTAAATACTTGCTACAACTTCAGCAAGTCTCTATCAAGCAGCATCATAATCAGCATTTTCACTTAAATCTCCTTGATTa    >  1:1000060/1‑128 (MQ=255)
                                                                                                                          ttAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACTTAAATCTCCTTGATTACg  >  2:2695149/1‑128 (MQ=255)
                                                                                                                          ttAAAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACTTAAATCTCCTTGAGTACg  >  1:925010/1‑128 (MQ=255)
                                                                                                                             |                                                                                                                            
TTAGATTAGAAAAAGGATCAGCTTCAATAGCACCAACAATTTTAATTTCTTTTAATTTATTATCTAAACTTGAAACAAATTGTACAGTTGATCCAATTTTAACTTCACCAGTTGAATGAGTTTTAGAATCATCAATAACTTTAGCTCTATTAATTAAAGTTTCAACTTCTTTAATTCTTGCTTCAACTTCAGCTTGTCTGTTTCTAGCAGCATCATAATCAGCATTTTCACTTAAATCTCCTTGATTACG  >  CP016816/153269‑153518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: