Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,222 G→A intergenic (+186/‑356) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2220GA98.6% 7076.8 / 66.2 2347intergenic (+186/‑356)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (1486/803);  minor base . (4/29);  total (1493/854)
Fisher's exact test for biased strand distribution p-value = 6.76e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2412 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349
                                                                                                                             |                                                                                                                                
cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                >  1:6776054/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAATAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               >  2:4485050/1‑128 (MQ=255)
 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                               <  1:201906/128‑1 (MQ=255)
  ttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatt                                                                                                                              >  1:5223713/1‑128 (MQ=255)
   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                             >  1:4086658/1‑128 (MQ=255)
   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                             >  2:2424793/1‑128 (MQ=255)
       tGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                         <  1:967833/128‑1 (MQ=255)
        gTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                        >  2:4220190/1‑128 (MQ=255)
              ctttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTg                                                                                                                  <  2:4355086/128‑1 (MQ=255)
               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGt                                                                                                                 >  2:4745214/1‑128 (MQ=255)
                 tactAGTTGATAATATTTAGTTTCATTAATTTATCTCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                               >  2:1545395/1‑128 (MQ=255)
                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                             <  1:2485891/128‑1 (MQ=255)
                     aGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaaa                                                                                                           >  1:2266569/1‑128 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                       >  2:884715/1‑128 (MQ=255)
                         gATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGc                                                                                                       >  2:1150367/1‑128 (MQ=255)
                          ctaataTTTAGTATCATTAATTTATCGCCCTTGTATTAATTAGTAAGAAACTAAAACTTTTATCTTTTTAGATATTTTTTGTTTATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGcg                                                                                                      <  2:6446659/127‑2 (MQ=255)
                           taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtt                                                                                                    >  1:3973715/1‑128 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAAATTTTAAAAAATAGAttt                                                                                                   >  1:6205322/1‑128 (MQ=255)
                            aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                   >  2:171273/1‑128 (MQ=255)
                                 ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttt                                                                                              >  2:5235872/1‑128 (MQ=255)
                                 ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttt                                                                                              >  2:6814070/1‑128 (MQ=255)
                                  tAGTTTCATTTATTTATCGCACTTGTTTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttt                                                                                             >  1:6862249/1‑128 (MQ=255)
                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttt                                                                                             >  2:3524344/1‑128 (MQ=255)
                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttt                                                                                           >  2:2113447/1‑128 (MQ=255)
                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttt                                                                                          >  1:6432508/1‑128 (MQ=255)
                                       taatttatttATCGCAATTGTATGAATTAGTAAGAAAATAAAACTATTATCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAAAAGCtttttttttttttt                                                                                        <  1:422561/126‑1 (MQ=255)
                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttattttttttttt                                                                                        >  2:3797270/1‑128 (MQ=255)
                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttt                                                                                       >  1:3634230/1‑128 (MQ=255)
                                        cATTAATTTATCGCACGTGTAGGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTAGATGCAAATTTTTAAAAAATTAAATAGAACTTGTAAACACTAGCttttttttttttttt                                                                                       >  1:3843053/1‑128 (MQ=255)
                                         atttatttTTAGCAATTTTATTTATTATTTATAAAATAAAAATTTTTTATTTTTATATATTTTTTTTATATGCAAAATTTTTAAAAATTAATTTGAACTTGTAAAAAAAATCtttttttttttttttt                                                                                      <  2:594234/128‑1 (MQ=255)
                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                      >  1:2612143/1‑128 (MQ=255)
                                                 atagCACTTTTATTAATTTTTATTAAAATAAAAATTTTTTTTTTTTTTTTATTTTTTTTATATTAAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAAATTTTTTTTTTTTTTTTTTTTTATAATg                                                                             <  2:2705312/125‑1 (MQ=255)
                                                 aTCGAAATTTTATTAATTATTTATAAAATAAAACTATTATATTTTTAGATATTTTTTGTATATTAAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATATCTTTTTTTTTTTTTTTTtataatgg                                                                              <  1:2912751/128‑5 (MQ=255)
                                                  tttcACTTTTTTTAATTATTAATAAAATAAAAAATTTATATTTTTTTTTTTTTTTTTTTTATTTAAATTTTTAAAAAATTTATTTTTAAATTTTAAAAATAGCTTTTTTTTTTTTTTTTTTATAATgg                                                                            <  2:2242778/125‑1 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttattatgta                                                                            >  2:292460/1‑120 (MQ=255)
                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttataatgtaaa                                                                            >  2:2539209/1‑118 (MQ=255)
                                                       cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtaatttaaattt                                                                        >  1:3727035/1‑118 (MQ=255)
                                                        ttttttGAATTTGTAAGAAACTAAAACTATTATATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATaa                                                                    <  1:3295706/123‑1 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAata                                                                       >  2:5346907/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATATAATGGAAttat                                                                      >  2:3952258/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAAtata                                                                     >  2:312747/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATaa                                                                    >  1:3166353/1‑128 (MQ=255)
                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGATTTTTTTTTTTTTTTTTTATAAtttaaaata                                                                      >  1:1876306/1‑120 (MQ=255)
                                                         tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTAATTATAATTTAAatat                                                                      >  1:1537653/1‑128 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCTTTTTTTTTTTTTTTTTTaatatgaaaaataaaa                                                                    >  1:4429832/1‑116 (MQ=255)
                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAATAATGTAAATATaaa                                                                   >  1:4891835/1‑128 (MQ=255)
                                                            aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTATAATATTTAAATATaatt                                                                  >  2:41118/1‑126 (MQ=255)
                                                              taaTTAGTAATAAAAATAAAATAATTTATTTTTTTTTATTTTTTTTATATTAAAATTTTTAAAAAAATAATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtttt                                                               <  1:4631450/127‑1 (MQ=255)
                                                                attttttataaaataaaaataTTTTTATTTTATATATTTTTTTTATTTTTAAATTTTTTAAAAATTTATTTTTAATTTTTAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtttt                                                               <  1:1143239/119‑1 (MQ=255)
                                                                 ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTATATTTAAATTTTAgt                                                            >  2:2324709/1‑127 (MQ=255)
                                                                  tAGTAAGAAACTACAACTATTATGTTTTTAGATATTTTTTGGATATGCAAATTTTTAAAATAATAATTTGAACTTGTAAATAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAATTTTTTTGTAt                                                         >  2:3667661/1‑128 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAATAATAGC‑‑TTTTTTTTTTTTTTTTTATATTTTAAATAAAAATTTTAgtt                                                           >  1:853534/1‑126 (MQ=255)
                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGGAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATTGGAAATATAAATTTTAgt                                                            >  1:5579625/1‑127 (MQ=255)
                                                                   attaataaACTAAAAATATAATCTTTTTAGATATTTTTTTTATATGGAAATTTTTAAAAAATTAATTTTTACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                           <  1:539458/126‑1 (MQ=255)
                                                                    ttattaaattaaaaataTTATTTTTTTTTTTAATTTTTTTTTATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAAAGTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                           <  1:5822045/123‑1 (MQ=255)
                                                                    ttaataaACTTAAATTATTATTATATTATATATTTTTTTTTTATTCATATTTTTAAAAAATTAATTTTTAATTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                         <  2:2007827/127‑1 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATTTAAAAATAAAattttttt                                                           >  2:4615774/1‑124 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAAtttaaatt                                                           >  1:6643427/1‑123 (MQ=255)
                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTTTAAATAAAAATTTTAGTTa                                                          >  1:4532967/1‑128 (MQ=255)
                                                                        gAAAATTAAAATATTATATTTTTATAATATTTTTGTATATGCAAATTTTAAAAAAAATTATTTGAACATGTAAAAAAATACTTTTTTTTTTTTTTTTTTATATTGGAAATATAAATTTTAGGTAtttt                                                      <  2:5475505/128‑1 (MQ=255)
                                                                         taaaaaaaaataATATATTTTTATATTTTTTTTTTTTATTTTAATTTTTATAAAATTATTTTTTATTTTTAAAAAAATTTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                     <  2:1877930/123‑1 (MQ=255)
                                                                           actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTtttttg                                                  >  1:6525624/1‑128 (MQ=255)
                                                                            ctaaaactaTTAACTTATTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTAGAACTTTTAAAAAATAGCTTTTTTTTTTTTTTTTATAAAATAAAAATAAAAATtatatttttagtatta                                                  >  2:673023/1‑116 (MQ=255)
                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTGTTTTCTaa                                             >  1:51217/1‑128 (MQ=255)
                                                                                aattattatTTTTTTTTATATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                             <  2:6181628/125‑1 (MQ=255)
                                                                                  ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTAATTTTTTTTTATaa                                             >  1:2384155/1‑128 (MQ=255)
                                                                                  atattttTTTTTTATTTATTTTTTTTTTTTTTAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                            <  1:3073187/127‑1 (MQ=255)
                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGAAAATATAAATTTTAGTTATTTTGTTGTATAATTa                                          >  2:3019929/1‑128 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAT‑‑TGTAAATATAATTTTTTGTTATTTtgttgtgaatgtat                                         >  2:5129916/1‑120 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATGTAAATATAAATTTTATGTATTTTTTTGTTTattta                                          >  2:3426820/1‑124 (MQ=255)
                                                                                    attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAAAATAAATTTTGTGTTTTTTGTTGTATAATTAt                                         >  2:6066784/1‑128 (MQ=255)
                                                                                         cTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTTTTTTTTTTTATAATTATATaa                                     >  2:3297456/1‑128 (MQ=255)
                                                                                         cTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                                 <  1:5470583/115‑1 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTAATTTTTTTTTATAATTATATaaaa                                   >  1:1001558/1‑128 (MQ=255)
                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAATTATAAATTTTAGTTTTTTTTTTGTGTATTTTTCTaaaaa                                  >  1:1345104/1‑128 (MQ=255)
                                                                                              tAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGCATTTTGTTGTCTAATTATCTaaaaaaa                                >  1:5976495/1‑128 (MQ=255)
                                                                                               ttatatTTTTTTTAATTTGAATTTTTTAAAAAATTAATTTGAAATTGTTAAAAAATTGTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAAt                               <  2:753503/126‑1 (MQ=255)
                                                                                                ttttttttttttttATTCTAATTTTTAAAAAATTTTTTTTTAATTTTTAAAATTATTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTACGTATCTAAAAAAATa                              <  1:6323900/124‑1 (MQ=255)
                                                                                                tttatTTTTTTTTTTTTAAAATTTTTTAAAAATTTATTTTTAATTTTAAAAAAT‑‑TTCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                             <  1:1763191/126‑1 (MQ=255)
                                                                                                 atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTAATGGAAATAAAAATTTTAGTTATTTTGTTGTATAAGTTTATAAAAAAATa                              >  2:5678559/1‑128 (MQ=255)
                                                                                                    ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATGTATTTTGTTTAATAATTTTCTAAAAAAATata                            >  1:1246101/1‑126 (MQ=255)
                                                                                                      ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAATTGTAAATAAAAATTTTAGTAATTTTTTTTTATAATTAAATAaaaaaaaaaaaata                        >  2:6619785/1‑126 (MQ=255)
                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTGTATAATTATATAAAAAAATATaaataaaa                     >  1:5030760/1‑123 (MQ=255)
                                                                                                            ttatttaaaTTTTTAAAAAATTTATTTTTAATTTTTAAAAAT‑‑TTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  2:5033150/122‑1 (MQ=255)
                                                                                                            attttaaaaTTTTTAAAAAATTAATTTTAAATTTTAAAAATATTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  1:568866/122‑1 (MQ=255)
                                                                                                            atctaaataTTTTAAAAAATATATTTTTAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata                <  2:1310114/120‑1 (MQ=255)
                                                                                                             tttttaaaTTTTTAAAAAATTAATTTTAAATTGTAAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  1:6108030/123‑1 (MQ=255)
                                                                                                             tatTCAAATTTTTAAAAAATTAATTTTTACTTTTAAAAAAAATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                 <  1:5321505/128‑1 (MQ=255)
                                                                                                             tatGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATATTAAATTAAAAATTTTTGTTATTTTGTTGTATATTTATATAAAAAAATTTAAAGTAATAAat                 >  1:2128351/1‑128 (MQ=255)
                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTAGGTATTTTTTTGTATAAGTTTATAAAAAAATATAAATTaaaaaaaa                >  1:3543678/1‑126 (MQ=255)
                                                                                                                 taaaTTTTTTAAAAAATATTTTGAACTTTTAAAAAATTTCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATAtt              <  1:3421078/127‑1 (MQ=255)
                                                                                                                 cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAAAGTAAAAATAATTTTTATTTATTTTTTTGTAAAAATTTATAAAAAAAAAAAAAGTAAAAAAAAtt              >  2:5556998/1‑128 (MQ=255)
                                                                                                                 cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATTATGGAAATATAATTTTTATGTATTTTTTTTTCTATGTTTATAAAAAAATAAAAAGAAAAAAAttttt             >  1:4761738/1‑127 (MQ=255)
                                                                                                                 cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTAATGTATTTTGTTGTCAAAGAATAAAAAAAAATATAAAGGAAAAAATAATGtt           >  2:1437180/1‑128 (MQ=255)
                                                                                                                   aaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTTATTTTTTTTTTTTTATAATTATATAAAAATATAGTAATGAAAAAGTAttaaat          >  1:1429520/1‑124 (MQ=255)
                                                                                                                    aTTTTTTAAAAATTAATTTTTAATTTTAAAAAAAATTTTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATGTATTTTTTTTTATAACTATAAAAAAAAATAGAAAGGAAAAAATATTGTTa          <  1:2750967/128‑1 (MQ=255)
                                                                                                                    aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAAATAAAATAAAAATTTAATTTATTTTTTTTTAAAATAAAAAAAAAAAATAAAAATAAAAAAATAttaaaa          >  2:57990/1‑124 (MQ=255)
                                                                                                                        ttaaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTGTGTAATTATATAAAAAATAAAAAAGCAAAAAAAATTTTTaaaa       >  2:6303707/3‑128 (MQ=255)
                                                                                                                         taaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTAAAAAATTAAATAAAAATTTTATTTATTTTATTTAAAAATTAAATAAAAAATAAAAAAATAAAAAATATTTATaaaaat     >  2:4854657/2‑126 (MQ=255)
                                                                                                                         taaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATTAAATAATAAATATATTTTTTTTTGTTTTATAAGTATATAAAAAAAAAAAAAAGAAAATAAATTTTTaaattt     >  1:1658675/2‑125 (MQ=255)
                                                                                                                             |                                                                                                                                
CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAAT  >  CP016816/165097‑165349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: