Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 242,857 G→A G829D (GGT→GAT)  JCVSYN2_01035 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816242,8570GA99.8% 9736.2 / NA 3033G829D (GGT→GAT) JCVSYN2_01035ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (1445/1577);  minor base C (6/1);  total (1451/1582)
Fisher's exact test for biased strand distribution p-value = 6.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3033 total aligned reads displayed.

AAAGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGGTTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGATTAAT  >  CP016816/242731‑242981
                                                                                                                              |                                                                                                                            
 aaGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGAtt                                                                                                                            <  1:5303923/128‑1 (MQ=255)
    taCTTGATTTAAAAAAGCTAAGTTTACTAATAAATTTAGTTTAACTTTAGCTTCTTATGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAg                                                                                                                         <  1:4774454/127‑1 (MQ=255)
       ttGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCaa                                                                                                                      <  1:440673/128‑1 (MQ=255)
        tGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAt                                                                                                                     <  1:3381467/128‑1 (MQ=255)
         gATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAtt                                                                                                                    >  2:4651067/1‑128 (MQ=255)
         gATTTAAAAAAGCTAAGTTTACTACTAAATATAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGGTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAtt                                                                                                                    <  2:3808043/128‑1 (MQ=255)
          ctttAAAAAACCTAAGATTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTAATAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAAttg                                                                                                                   <  2:4877748/127‑2 (MQ=255)
          aTTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTc                                                                                                                   >  1:644831/1‑128 (MQ=255)
          aTTTAAAAAAGCTAAGTTTAATACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTAATTAGTAACTGTAGTTGTATTTTCAAGTATGTTTATTTCAGCAGATTTAGCAATTc                                                                                                                   >  1:994525/1‑128 (MQ=255)
            ttAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCa                                                                                                                 <  2:2538005/128‑1 (MQ=255)
            ttAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCa                                                                                                                 <  2:4200330/128‑1 (MQ=255)
            ttAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCa                                                                                                                 <  1:1357386/128‑1 (MQ=255)
               aaaaaGCTAAGATTACTACTAAATTTAGTATAACTTTATCTTAATCTCGAAAAAAAAAAATCATATTAATATTAAATGTTGATGGAAAATCAACAATAATTATTAAAACAGATTAAGCAATTCAATCa                                                                                                              >  1:6060637/1‑128 (MQ=255)
                        actttACTACTAGATTAAGTTTAACTTTAGCATCTTCTGGGAAAAAAAATATCTTTTTATTAGTAACGGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCtttt                                                                                                     <  2:1920277/126‑1 (MQ=255)
                        aGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTATCAATTCCATCAATTGCtttt                                                                                                     >  2:2032201/1‑128 (MQ=255)
                         gTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTa                                                                                                    <  2:1523686/128‑1 (MQ=255)
                          tttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTAc                                                                                                   >  1:1212671/1‑128 (MQ=255)
                          tttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTAc                                                                                                   >  2:1660371/1‑128 (MQ=255)
                          tttACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTAc                                                                                                   >  1:4052950/1‑128 (MQ=255)
                            tactacTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTa                                                                                                 >  2:3533997/1‑128 (MQ=255)
                            tactacTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTa                                                                                                 >  1:5942577/1‑128 (MQ=255)
                             actactAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTAt                                                                                                >  1:2413217/1‑128 (MQ=255)
                             actactAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTAt                                                                                                <  1:1896319/128‑1 (MQ=255)
                              ctactaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTAtt                                                                                               >  2:4537872/1‑128 (MQ=255)
                              ctactaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTAtt                                                                                               >  1:5550175/1‑128 (MQ=255)
                              ctactaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGATTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTAtt                                                                                               >  1:2038669/1‑128 (MQ=255)
                                actaAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaa                                                                                             <  1:1789776/128‑1 (MQ=255)
                                actaAATTTAGTTTAACTTTAGATTATTCTGGTAAAAAATTTTTCTTTTTATTAGTAACTGTTGTTGGAAATTCATCTATGTTTATTTCAGCAGATATAGCAATTCCATGAATTGCTTTAACTATTaa                                                                                             <  2:5644794/128‑1 (MQ=255)
                                 cttaaTTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaa                                                                                            <  2:846073/125‑1 (MQ=255)
                                  taAATTTAGTTTAACTTTATCTTCTTCTGTTAATAAAAATATCTTTTTATTATTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaaa                                                                                           <  1:4296858/128‑1 (MQ=255)
                                   aaaTTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTATACTATTaaaaa                                                                                          >  1:109648/1‑128 (MQ=255)
                                      tttAGTTTAATTTTAGCTTCTTATTTTAAAAAAAATATATTTTTATTAGTTACTGTTTTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAacac                                                                                       <  2:1790491/128‑1 (MQ=255)
                                             tAACTTTAGCTTCTTCTCGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTtattat                                                                                <  1:682898/128‑1 (MQ=255)
                                               aatttAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTa                                                                                              <  1:6569884/110‑1 (MQ=255)
                                               aCTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTaaaaa                                                                                          >  2:367152/1‑116 (MQ=255)
                                               aCTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaa                                                                              <  1:5418310/128‑1 (MQ=255)
                                               aCTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaa                                                                              >  2:6282295/1‑128 (MQ=255)
                                               aCTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaa                                                                              >  1:1699105/1‑128 (MQ=255)
                                                cTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATaaa                                                                             >  2:3952994/1‑128 (MQ=255)
                                                   tAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGt                                                                          <  2:3200733/128‑1 (MQ=255)
                                                    aGCTTCTTCTGGTAAAATAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTAAACTATGTTTATTTCAGCAGATTTAGCAATTACATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTa                                                                         <  1:3618994/128‑1 (MQ=255)
                                                       ttcttcTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTa                                                                      <  1:2746111/128‑1 (MQ=255)
                                                         cttctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTaaa                                                                    >  2:749701/1‑128 (MQ=255)
                                                         cttctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTCTAGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTACATCAATTGCTATTACTATTAAAAACACTAATTATAAAAGTAATaaa                                                                    >  2:3429810/1‑128 (MQ=255)
                                                          ttctTGTAAAAAAAATATCTTTTTATTAGTAACTGTTCTTGGAATTTAAACTATGTTTATTTCATCAGATTTATCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAat                                                                   <  2:4780898/128‑1 (MQ=255)
                                                           tctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAata                                                                  >  2:1278491/1‑128 (MQ=255)
                                                            ctGGTAAAAATAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCATCTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAatat                                                                 <  1:5139057/128‑1 (MQ=255)
                                                            ctGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAatat                                                                 <  2:443048/128‑1 (MQ=255)
                                                            ctGGTAAAAAAAATATCTTTTTATTAGTAAATGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAatat                                                                 <  1:4905072/128‑1 (MQ=255)
                                                              ggTAAAAAAACTATCTAATTATAAGTAACTGTTGTAGGAATTTCAACAAAGTTTATATCATAACATTTAGAAATTAAATCAATTGATATTACTATTAAAAACTCTAAATATAAAAGTATTAAATATga                                                               >  1:4171812/1‑127 (MQ=255)
                                                              ggTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGc                                                               <  1:6366771/128‑1 (MQ=255)
                                                                tAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTAGAAAAGTATTAAATATGCaa                                                             >  2:2274013/1‑128 (MQ=255)
                                                                 aaaaaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCaaa                                                            <  1:6705545/128‑1 (MQ=255)
                                                                  aaaaaaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAAt                                                           <  2:3301600/128‑1 (MQ=255)
                                                                      aaaTATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAat                                                       <  2:6157541/128‑1 (MQ=255)
                                                                        atatCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAatat                                                                 >  2:4284615/1‑116 (MQ=255)
                                                                         tatCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAAtata                                                    <  2:5237143/128‑1 (MQ=255)
                                                                           tCTTTTTATTAGTAACTGTTGTTTGAATTTCAACTATGTTGATTTCATCAGATTTAGCAATTCCATCAATTGATTTTAATATTAAACAAAAGTATTATAAAAGTATTAAATAAGCAAATGAATATAat                                                  >  1:3521908/1‑128 (MQ=255)
                                                                            cTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAatt                                                 <  1:3268895/128‑1 (MQ=255)
                                                                             tttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAatta                                                <  2:2947452/128‑1 (MQ=255)
                                                                              ttttATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAattat                                               <  2:6806028/128‑1 (MQ=255)
                                                                                ttattaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCATATGAATATAATTATTc                                             >  1:3159570/1‑128 (MQ=255)
                                                                                ttattaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTc                                             <  1:3738117/128‑1 (MQ=255)
                                                                                ttattaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTc                                             >  2:2406821/1‑128 (MQ=255)
                                                                                 tattaGAAACTGTTGTTGGAAATTCAACTATGATTAATTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCa                                            <  2:2796236/128‑1 (MQ=255)
                                                                                  attaGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCaa                                           >  1:2183104/1‑128 (MQ=255)
                                                                                    taGTAACTGTTGTTGGAATTTAAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCaaaa                                         <  1:2644226/128‑1 (MQ=255)
                                                                                     attAACTGTTGTTGGAATTTCTACTATTTTTATTTCAGCTGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCATATGAATATAATTATTCAAAAg                                        <  2:4058803/126‑1 (MQ=255)
                                                                                      gTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAgg                                       <  2:1109848/128‑1 (MQ=255)
                                                                                      gTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAATATGCAAATGAATATAATTATTCAAAAGgt                                      <  2:1393563/128‑1 (MQ=255)
                                                                                          cTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   <  2:6505481/128‑1 (MQ=255)
                                                                                          cTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGtt                                   >  1:159873/1‑128 (MQ=255)
                                                                                            gttgttTGAAATTCAACTATGTTTATTTAATCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTc                                 <  1:1481627/128‑1 (MQ=255)
                                                                                            gttgttGGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTc                                 >  1:761089/1‑128 (MQ=255)
                                                                                                ttgGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAAt                             >  1:5017367/1‑128 (MQ=255)
                                                                                                 tgGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACttat                                                                                   <  2:2656395/73‑1 (MQ=255)
                                                                                                 tgGAATTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAAtt                            >  2:6649374/1‑128 (MQ=255)
                                                                                                    aaTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtctc                         >  1:6252456/1‑128 (MQ=255)
                                                                                                    aaTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATtctc                         >  2:3057873/1‑128 (MQ=255)
                                                                                                     aTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGTTGTTTCAAAATCTcc                        >  1:4510477/1‑128 (MQ=255)
                                                                                                     aTTTCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGCGTTTCAAATTCTcc                        <  2:2157162/128‑1 (MQ=255)
                                                                                                      tttCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCt                       <  1:2515723/128‑1 (MQ=255)
                                                                                                      tttCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCt                       <  1:3010656/128‑1 (MQ=255)
                                                                                                      tttCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCt                       <  2:6051151/128‑1 (MQ=255)
                                                                                                       ttCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCtt                      >  1:6181679/1‑128 (MQ=255)
                                                                                                       ttCAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATACTCCtt                      >  2:5354169/1‑128 (MQ=255)
                                                                                                         cAACTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGTTGTTTCAAATTCTCCTTTa                    >  1:863006/1‑128 (MQ=255)
                                                                                                            cTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAATTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACa                 <  2:551727/128‑1 (MQ=255)
                                                                                                            cTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCt                       >  1:2893215/1‑122 (MQ=255)
                                                                                                            cTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACa                 <  1:5666783/128‑1 (MQ=255)
                                                                                                            cTATGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACa                 >  1:2301887/1‑128 (MQ=255)
                                                                                                              aTGTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACaaa               >  2:5926768/1‑128 (MQ=255)
                                                                                                                gTTTATTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAAc             >  1:1002962/1‑128 (MQ=255)
                                                                                                                 tttATTTCAGCAGATTTAGTAATTACATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAAcc            <  1:5805062/128‑1 (MQ=255)
                                                                                                                    aTTTCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAAc         >  1:3869885/1‑128 (MQ=255)
                                                                                                                     cttCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACg        >  2:4968778/2‑128 (MQ=255)
                                                                                                                       tCAGCAGATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAATGGTGTTTCAAATTCTCCTTTAACAAAACCAACGat      >  2:5778784/1‑128 (MQ=255)
                                                                                                                        cagcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGatt     <  2:5103158/128‑1 (MQ=255)
                                                                                                                         agcagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGatta    <  2:6386869/128‑1 (MQ=255)
                                                                                                                           cagATTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGattaat  <  1:4672016/128‑1 (MQ=255)
                                                                                                                              |                                                                                                                            
AAAGAACTTGATTTAAAAAAGCTAAGTTTACTACTAAATTTAGTTTAACTTTAGCTTCTTCTGGTAAAAAAAATATCTTTTTATTAGTAACTGTTGTTGGAATTTCAACTATGTTTATTTCAGCAGGTTTAGCAATTCCATCAATTGCTTTTACTATTAAAAACACTTATTATAAAAGTATTAAATATGCAAATGAATATAATTATTCAAAAGGTGTTTCAAATTCTCCTTTAACAAAACCAACGATTAAT  >  CP016816/242731‑242981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: