Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816420,2341.T45.1% inf / inf 1256intergenic (‑517/+326)JCVSYN2_01895/JCVSYN2_0190030S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base . (410/278);  new base T (427/139);  total (837/419)
Fisher's exact test for biased strand distribution p-value = 3.17e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-14

Only 100 of 1500 total aligned reads displayed.

TTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAATTAAATATATAACAATGTAAGTTTTAAAA  >  CP016816/420107‑420362
                                                                                                                                              |                                                                                                                                     
ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑g                                                                                                                                        >  2:4123433/1‑128 (MQ=255)
 tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑t                                                                                                                                     >  2:4197929/1‑128 (MQ=255)
  gtattttttctaatatTACAAAAATAATACCAATTTATTTAAAAAATATTATTTTTAAAAAATAAAAAATTTTTCAAATTGTTAAATTATTTTCAAAACATTATTTATTAATAAA‑‑‑ATTTATGC‑‑GC‑‑‑G‑‑tt                                                                                                                                    <  2:310728/116‑1 (MQ=255)
   tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTATATTATTTTCAATTCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑ttt                                                                                                                                   >  1:3489842/1‑128 (MQ=255)
   tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑ttt                                                                                                                                   >  1:4849803/1‑128 (MQ=255)
   tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑ttt                                                                                                                                   >  1:1940029/1‑128 (MQ=255)
     ttaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTATATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑ttttt                                                                                                                                 >  2:2839523/1‑128 (MQ=255)
         ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑ttttttttt                                                                                                                             >  1:5513949/1‑128 (MQ=255)
         ttGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑ttttttttt                                                                                                                             >  1:1698682/1‑128 (MQ=255)
             tatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑ttttttttttttt                                                                                                                         >  2:4676133/1‑128 (MQ=255)
              atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑tttttttttttttt                                                                                                                       >  1:2541848/1‑128 (MQ=255)
              atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑tttttttttttttt                                                                                                                       >  1:4339199/1‑128 (MQ=255)
                 ttattaaaCTAAAATCAATTTTTTAAAATATTTTTATATTTAATAAATGATATATTTTTCAAATTGTTAAATTTTTTTAAAATCATTATCTTTTAATTAA‑‑‑TTTTATGC‑‑GC‑‑‑G‑‑ttttttttttttttttt                                                                                                                   <  2:5235619/123‑1 (MQ=255)
                   aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATTA‑‑TC‑‑‑T‑‑ttttttttttttttttaaa                                                                                                                  >  2:4993834/1‑125 (MQ=255)
                      aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATAAA‑‑‑TTTTATTC‑‑TC‑‑‑T‑‑TTTTTTTTTTTTTTTTTAaaaa                                                                                                             >  1:3574004/1‑128 (MQ=255)
                      aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTATCAAATCATTATCTATTAATCAA‑‑‑TTATATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTAATTTACaa                                                                                                              >  1:5324381/1‑128 (MQ=255)
                      aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTATTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑ttttttttttttttttttttaa                                                                                                              >  1:1437704/1‑123 (MQ=255)
                       aaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTTAaaaa                                                                                                            >  2:2775870/1‑128 (MQ=255)
                         ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑T‑‑TTTTTTTTTTTTTTTTTAAAAAAat                                                                                                          >  1:3998327/1‑128 (MQ=255)
                          tactaTCAATTTTTTAAAAAATTTTTATTTTTAAAAACTTAATTTTTTTTTAAATTGTTAAATTATTTTCAAATCATTATATATTTATAAA‑‑‑TTTTATTC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTACAAAAat                                                                                                          <  2:4458914/128‑1 (MQ=255)
                          tactaTCAATTTTTTAAAAAAATTTTAATTTTAATAACTGAATTATTTTTAAAATTGTTAAATTATTTTAAAATAATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTACAAAAat                                                                                                          <  1:4757105/128‑1 (MQ=255)
                          tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑ttttttttttttttttttaacaaaaa                                                                                                          >  2:4918045/1‑119 (MQ=255)
                          tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTTaaaaaaat                                                                                                         >  1:1892565/1‑126 (MQ=255)
                          tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTAAAAAAatt                                                                                                         >  1:5087861/1‑127 (MQ=255)
                          taatatcACTTTTTTAAAAAAATATTATATTTTATAAATTAATTATTTTTTAAATTTTTAAATTTATTTAAAATCATTATAAATTAATAAA‑‑‑TTTTTTGG‑‑TC‑‑‑G‑‑GTTTTTTTTTTTTTTTTACAAAAata                                                                                                         <  1:3878015/122‑1 (MQ=255)
                           cattcaatttttttAAAAAATTTTAATTTTAATAACTGTATTATTTTTGAAATTTTTAAATTATTTTTAAATACTAATAAATTAAACAA‑‑‑ATTTATGC‑‑TG‑‑‑GGTTTTTTTTTTTTTTTTTTACAAAAata                                                                                                         <  1:4113931/120‑1 (MQ=255)
                            ttttaaattttttAAATAATTTTTTTTTTTAATAAATTAATTTTTTTTAAAATTTTTAAATTATTTTAAAATATTTTTATTTTTATAAA‑‑‑TTTTATTT‑‑TT‑‑‑T‑‑TTTTTTTTTTTTTTTTTACAAAAAtata                                                                                                      <  2:118200/121‑1 (MQ=255)
                            ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTTTAAAAAaata                                                                                                      >  1:1801404/1‑128 (MQ=255)
                            ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTTAAAAAAaata                                                                                                      >  1:3255494/1‑128 (MQ=255)
                             tatcACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATCATTATCTATTAATAAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATaa                                                                                                     <  1:5041255/125‑1 (MQ=255)
                             taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTTaaaaaaattta                                                                                                      >  1:595332/1‑123 (MQ=255)
                              tttaattttttTAAAAATTTTTTATTTTTATAAATTTATTATTTTTTAAATTTTTTAATTTTTTTTAAATAATTATAAATTTATAAA‑‑‑TTTTTTTC‑‑TT‑‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAat                                                                                                    <  1:1002565/123‑1 (MQ=255)
                               gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTTTAAAAaataataa                                                                                                  >  2:680027/1‑126 (MQ=255)
                               gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTTAAAAAAAATaaaa                                                                                                   >  1:2724269/1‑126 (MQ=255)
                               gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TATTATGC‑‑TA‑‑‑G‑‑TATTTTTTTTTTTATTTAaaaaaaaaataaa                                                                                                    >  1:787302/1‑121 (MQ=255)
                                 aCTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATATAattt                                                                                                  >  2:5172690/1‑128 (MQ=255)
                                  cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATAAAtttta                                                                                                 >  1:5201988/1‑128 (MQ=255)
                                  cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTTAAAAAAAATAaattaa                                                                                                >  1:4950019/1‑127 (MQ=255)
                                  cTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATCTATTAATCAA‑‑‑TTTTATTC‑‑TC‑‑‑‑‑TTATTTTTTTTTTTTTTAAAAAAATAAAatttatt                                                                                               >  1:1996584/1‑128 (MQ=255)
                                   ttttttAAAAAATTTTTTATTTTAATTAATTTATTATTTTTTAAATTTTTAAAATATTTACAAATTAATAATTAATAATAAATTTTTTTGTGG‑‑TT‑‑‑T‑‑TTTTTTTTTTTTTTTTTACAAAAATATAattt                                                                                                  <  2:1727344/128‑1 (MQ=255)
                                   ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTAAAAAAATTTAatttattt                                                                                              >  2:1914930/1‑128 (MQ=255)
                                   ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTAAAAAAATATAatttattt                                                                                              >  1:1085341/1‑128 (MQ=255)
                                   ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑‑‑TTTTTTTTTTTTTTTACAAAAATATAATTTATTTg                                                                                             >  1:3050656/1‑128 (MQ=255)
                                   ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTATATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTAAACAAAAAAATAatttat                                                                                                >  1:706764/1‑128 (MQ=255)
                                   ttttttAAAAAATTTTTAATTTTAATAAATTAATTATTTTTTAAATTTTTAAATTATTTTAAAATTATTATATATTAATCAA‑‑‑TTTTATTC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTACAAAAATATAatttat                                                                                                <  1:4197929/128‑1 (MQ=255)
                                   ttttttAAAAAATTTTTAATTTTAATAAATGAATTATTTTTAAAATTTTTAAATTTTTTTAAAATAATTATATATTAATAAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTACAAAAATATAatttattt                                                                                              <  1:895960/128‑1 (MQ=255)
                                       tttAAAAAATTTTTTATTTTAATTAATTTATTTTTTTTAATATTTTTTAATTATTTTAAAATAATTATATATTAATAAA‑‑‑TTTTATTC‑‑TA‑‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa                                                                                            <  1:2357613/128‑1 (MQ=255)
                                       tttAAAAAATTTTTAATTTTAATAAATTTATTATATTTCAAATTTTTAAATTATTTTTAAATAATTATATATTAATAAA‑‑‑TTTTATTC‑‑TA‑‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa                                                                                            <  1:340110/128‑1 (MQ=255)
                                             aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TCT‑‑T‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAATTTATttaattaa                                                                                        >  1:4893727/1‑128 (MQ=255)
                                             aaaaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTTTTttatta                                                                                         >  1:2942866/1‑128 (MQ=255)
                                               aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTAAAAAAAATAAATATTATTTTATTAAAc                                                                                      >  2:595063/1‑128 (MQ=255)
                                               aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGA‑‑TCT‑‑T‑‑TTTTTTTTTTTTTTTTTAAAAAAAAATAATTAATTTTAATaaaa                                                                                      >  1:3669968/1‑127 (MQ=255)
                                                 tttttaattttaatAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTAACAAAAATATTATTTATTTGATTAAACTta                                                                                   >  2:4736969/12‑128 (MQ=255)
                                                  ttttaattttaatAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTAAAAATATAAATTTTTTTTTTTTACACTtat                                                                                  >  2:1008481/11‑128 (MQ=255)
                                                  ttttaattttaatAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑‑‑TTTTTTTTTTTTTTTAAAAAAAAATAATTTAATTTATTTAAAGTaaat                                                                                >  1:2821931/11‑128 (MQ=255)
                                                        ttttaattaattaaTTATTTTTAAAATTTTTAAATTTTTTTTAAATAATTATTTATTAATAAA‑‑‑TTTTTTTC‑‑TA‑‑‑GTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTa                                                                             <  1:4424050/116‑1 (MQ=255)
                                                         tttaataatTTAATTATTTTTAAAATTTTTAAATTATTTTTAAATTATTATCTTTTAATAAA‑‑‑TTTTATGG‑‑TT‑‑‑GTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTa                                                                             <  2:4606463/125‑1 (MQ=255)
                                                         tttaatAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAAAATAATTTAATTAATTAAACTTttatttat                                                                            >  1:2458095/4‑128 (MQ=255)
                                                          ttaattacattatttttTTTTAAATTGTTTAAATATTTTTAAAACATTATCTAATAAACAA‑‑‑TTTTATGGC‑‑TGT‑‑‑T‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTAt                                                                            <  2:2408642/117‑1 (MQ=255)
                                                          ttaattaatttattattTTTTAAATTGTTAAATTATTTTTAAATAATTATATATTAATAAA‑‑‑TTTTATTC‑‑TCTTTT‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTa                                                                             <  1:4479861/117‑1 (MQ=255)
                                                           taatAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATATATTTTATTTTTTAAAAAGTATATTTAttt                                                                          >  2:536633/2‑126 (MQ=255)
                                                             tataattatttattTTTTAAATTTTTTTATTTTTTTAAAATTATTATATATTAATTAA‑‑‑TTTTTTTT‑‑CT‑‑‑T‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTta                                                                       <  1:4668197/119‑1 (MQ=255)
                                                             attaatttatttttTTTAAAATTTTTTAATTTTTTTAAAATAATTATATATTAATAAA‑‑‑TTTTATTG‑‑TA‑‑‑GTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATGttt                                                                        <  1:542433/120‑1 (MQ=255)
                                                             atAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTTATTAAACTTATTTTTTTTTTta                                                                       >  1:5255308/1‑128 (MQ=255)
                                                              taatttaaTTTTTTTTTAAATTTTTTAATTTTTTTTAAATAATTATTTTTTAATTAAT‑‑TTTTTTTT‑‑TT‑‑‑T‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTta                                                                       <  1:2608931/122‑1 (MQ=255)
                                                                 attttttttttttATAATTTTTTAATTTTTTTAAAATAATTATATTTAAATAAA‑‑‑TTTTTTGT‑‑TT‑‑‑T‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTtaataa                                                                   <  2:3530579/120‑1 (MQ=255)
                                                                  tGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTTATTAAAATTATATTTATTTTtaataa                                                                   >  2:4320721/1‑128 (MQ=255)
                                                                  tGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTATTAACCTTATTTTTATTTTtaataa                                                                   >  1:5140171/1‑128 (MQ=255)
                                                                    aaTTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTTTTTAAAAGTATATTTATGTTTAATaaaa                                                                 >  2:1485034/1‑128 (MQ=255)
                                                                     attattTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTTTTAAACATAAATTTTTTGTTAATtaaat                                                                >  2:4399967/1‑127 (MQ=255)
                                                                         ttttttAAATTTTTAAATTTTTTTTAAAATATTATATATAAAAAAAA‑‑‑TTTTATTCTTTT‑‑‑T‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATg                                                              <  2:4849803/128‑1 (MQ=255)
                                                                           ttttataTTGTTATATTATTTTATAATATTTTTTTTTTTATTAA‑‑‑TTTTTTTT‑‑TC‑‑‑T‑‑TTTTTTTTTTTTTTTTTACAATAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGaaaa                                                         <  2:2575437/122‑1 (MQ=255)
                                                                           tttCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTTAAAAAAATTTAATTTATTTTATTAAACTTATATTTATTTTTAATAAAAAtttaa                                                           >  1:4152560/1‑124 (MQ=255)
                                                                           tttCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTAACAAAAATATAATTTTTTTCATTAAACTAATATTTATTTTTAATAAAAATgttaa                                                          >  2:2932474/1‑124 (MQ=255)
                                                                            tttaaaTTTTTTAATTTTTTTAAAATTATTATTTTTTAATTAA‑‑‑TTTTTTTT‑‑TCT‑‑T‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGaaaa                                                         <  1:1240352/125‑1 (MQ=255)
                                                                             tCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTTTTTTATTAAAATTTTTTTTATTTTTAATAAAAATGTaaaa                                                         >  2:453389/1‑128 (MQ=255)
                                                                             tCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTAACAAAAATTTAATTTATTTTATAAAACTTAATTTTATTTTTAATAAAAATTAaaaat                                                        >  1:1311335/1‑127 (MQ=255)
                                                                               aaaTTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTATTAAACTTATATTTATTTTTAATAAAAATTTaaaata                                                       >  1:1371678/1‑126 (MQ=255)
                                                                                aaTTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTATTAAAAGTATATTTATTTTTAATAAAAATTTaaaaaaaat                                                    >  1:152093/1‑127 (MQ=255)
                                                                                    gTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTTTTTTATTAAACTTATATTTATGTTTAATAAAAATTTaaaaaaaattt                                                  >  2:4243047/1‑125 (MQ=255)
                                                                                      tAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTACAAAATTTTAATTTTTTTTATTAAAAGTATATTTATGTTTAATAAAAATGTAAAATAAATTGatt                                               >  2:3859913/1‑126 (MQ=255)
                                                                                         attattTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTATTAAAAGTATTTTTATTTTTAATAAAAATGTAAAAGAAATTTAATTGt                                            >  1:2890039/1‑128 (MQ=255)
                                                                                          ttattTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTTAAAAAAAATTAATTTATTTTATTAAATGTATTTTTTTTTTTTAAAAATATTTAAAATTAAGTGATTTGt                                            >  2:1604597/1‑128 (MQ=255)
                                                                                           tattTTAAAATAATTATATATTAATAAA‑‑‑TTTTATTA‑‑TT‑‑‑GTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAATTGTCa                                          <  1:4532196/128‑1 (MQ=255)
                                                                                             ttttTAAATAATTATATTTTAATTAA‑‑‑TTTTATTTT‑‑TT‑‑‑T‑‑TTTTTTTTTTTTTTTTTACATAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAATTGTCata                                        <  2:5094318/128‑1 (MQ=255)
                                                                                                  aaaTCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATATAACTTATTTATTTAAAATTATATTTATTTTTAATAAAAATGAAAAATAAATTAAATTATAAAaataat                                   >  2:3238093/1‑128 (MQ=255)
                                                                                                   aaTCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATAAAATTTATTTTATTAAAATTATATTTATTTTTAAAAAAAATGAAAAATAAATTAAATTTAAATAAtaatt                                  >  1:210827/1‑128 (MQ=255)
                                                                                                        ttATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATATATTTTTTTTTATTAAAATATTATTTATTTTTAATAAAAATTTAAAAAAAATTTAATTTTAAAAAtaatttattt                             >  1:117493/1‑128 (MQ=255)
                                                                                                            cTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTTATTAAACGTTTATTTTTGTTTAATAAAAATGGAAAAAAAAGTGAATTGAAAAAATAATTTAATaaaa                          >  1:4605708/1‑128 (MQ=255)
                                                                                                             tATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAAAATTAATTTTTTTAATTAAAAGTATTTTTTTTTTAAAAAAAATTTAAAAAAAAATTTAATGTAAATATAATTTTaataaata                        >  2:362/1‑128 (MQ=255)
                                                                                                              aTTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTTACAAAAATATAATTTTTTTTTTTAAAATTTATTTTATTTTTAATAAAAATTGAAATAAAAGTTAATTTTAATAATTATttatttaataa                        >  2:2068945/1‑125 (MQ=255)
                                                                                                              aTTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTATTTTATTAAACGTATATTTATTTTTAATAAAAATGTAAAATAAATTGAATTGTCATAATAATTTAATTAAata                        >  1:375370/1‑128 (MQ=255)
                                                                                                              aTTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATATAATTTTTTTTATTAAAGGTATATTTATTTTTAATAAAAATGAAAAATAAATTTAATTTTAATAATAATTTATTTAAatat                       >  1:656352/1‑128 (MQ=255)
                                                                                                               ttAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAATTTTAATTTTTTTTATTAAACGTTTTTTTTTGTTTATTAAAAATGTAAAAGAAATTGATTTTTAATATTAATTTAATTAAatatt                      >  1:2231119/1‑127 (MQ=255)
                                                                                                                  aaTCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTACAAAAATAAAATTTATTTTAATAAACGTTTATTTTTTTTTAATAAAAATGAAAAATAAATTGAATTTAAAAAAATATTAAAATAAAtatata                    >  2:1269115/1‑128 (MQ=255)
                                                                                                                    tCAA‑‑‑TTTTATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAATTATTTATTTAAAAGTAAATTTTTTATAAAATAAAATAAAATATAAATTTTATTTTAATAATAAATTATTTAAatattaatc                  >  1:4267385/1‑123 (MQ=255)
                                                                                                                            tttATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTAAAAAAATATATTTTTTTTTTTTAAACGTATTTTTTTTTTTAATAAAAATGTAAAATAAATTTATTTTTAAAAATAATTTAATTAAATATTTAAAAAttaa            >  2:1387556/1‑125 (MQ=255)
                                                                                                                              tATGC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTAAAAAAATTTAATTTATTTTATTAAACTTATTTTTTTTTTTAATAAAAATTGAAAAAAATTTTAATTTTAATATAAATTTATTTAAATATATAACAATTTaa           >  1:3170176/1‑128 (MQ=255)
                                                                                                                                   gC‑‑TC‑‑‑GTTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTTATTAAACGTATATTTTTGTTTAAAAAAAATTGAAAAGAAATTGAATTGTCATAATAATTTAATTAAATATAAAAAaatttaattt        >  2:5467586/1‑128 (MQ=255)
                                                                                                                                             g‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTTATTAAACGTATATTTATTTTTAATAAAAATGTAAAATAAAGTTAATTTTAATAATAATTTAATTAAATTTATAAAAATTTAATTTTTaaa   >  1:1584216/1‑128 (MQ=255)
                                                                                                                                              |                                                                                                                                     
TTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑‑TTTTATGC‑‑TC‑‑‑G‑‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAATTAAATATATAACAATGTAAGTTTTAAAA  >  CP016816/420107‑420362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: