Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP01681634,9451.A40.9% 830.7 / inf 2071intergenic (+49/+117)JCVSYN2_00105/JCVSYN2_00110ABC transporter permease/cell division protein FtsH
Reads supporting (aligned to +/- strand):  ref base . (463/758);  new base A (788/58);  total (1251/820)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-23

Only 100 of 2350 total aligned reads displayed.

CTTTACTTCTTTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTATTTGTCTT  >  CP016816/34818‑35072
                                                                                                                                      |                                                                                                                                        
cTTTACTTCTTTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑g                                                                                                                                           >  2:4719055/1‑128 (MQ=255)
       tcttTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑aaaaaaa                                                                                                                               >  1:3202720/1‑128 (MQ=255)
       tcttTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTATTTATTTACTATT‑‑G‑‑‑aaaaaaa                                                                                                                               >  1:418808/1‑128 (MQ=255)
            aTGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACAATT‑‑G‑‑‑aaaaaaaaaaaa                                                                                                                         >  2:3605108/1‑128 (MQ=255)
              taGTTTAAACAAAAATATTAATAAATGCTGAACCAAGTAAAATGCTAATTTATTTTTATTAATAATAAAATATAAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑T‑‑‑aaaaaaaaaaaaaa                                                                                                                       <  1:4761810/127‑1 (MQ=255)
               aGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGATTGGTTTTTTTATTTACTATT‑‑G‑‑‑aaaaaaaaaaaaaaa                                                                                                                      >  2:5286583/1‑128 (MQ=255)
                    caacaaAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTATGGTTTTTTTATTTACTATT‑‑A‑‑‑aaaaaaaaaaaaaaaaaaaa                                                                                                                >  1:1888579/1‑126 (MQ=255)
                       caaAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAaaaaaattt                                                                                                            >  1:614672/1‑126 (MQ=255)
                        aaaaaGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTTAATTTTATGAATAATAAAATATTAAACCAAATACATTTTGTTTTGTTATTTTATTTAATATT‑‑GA‑‑AAAAAAAAAAAAAAataatataa                                                                                                            >  1:796535/1‑128 (MQ=255)
                           aaGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑ttt                                                                                                       >  1:4142153/1‑128 (MQ=255)
                            agagTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTg                                                                                                      >  2:4424107/1‑128 (MQ=255)
                               gTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AACAAAAAAAAAAAAataatataa‑‑tataaaa                                                                                                   >  2:2418148/1‑122 (MQ=255)
                                tAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATTT‑‑A‑‑‑AAAAAAAAAAAAAAAAAATATAA‑‑ttaaaata                                                                                                  >  1:247327/1‑122 (MQ=255)
                                  aGAAATGCTGCACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAATATAA‑‑TTTGCATACt                                                                                                >  1:2925309/1‑128 (MQ=255)
                                   gAAATTCTTAACAAATTACAATGCTAATTGATTTTGATTAAAAATAAAATATTAAACAAAATAAATTGGTTTTGGTTTTTTTATTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACta                                                                                               <  2:409265/128‑1 (MQ=255)
                                   gAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAATATAA‑‑ttttaataatt                                                                                               >  1:3448215/1‑120 (MQ=255)
                                     attcgttaaCCAATTTAAATGCTAAGTTAAGTTTATTAATAATAAAATTATAAACAAAATACTTTTTTTTTGGTTTTTTTTTTTATTATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACtata                                                                                             <  2:614037/121‑1 (MQ=255)
                                     aattctttaCCAATTACAATTTTAATGTAAGTTTTTTAAAAATAAAATATTAAAACAAATAAATTGTGTTTGGTTTTTTTATTAACTATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACtata                                                                                             <  1:5072144/120‑1 (MQ=255)
                                     aaTGCTTAACAAAGTACAATGCTAAGTGAATTTTATTAATAAAAAAATATTAAACCAAATAAATTGTGTTTGTTTTTTTTATTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACtata                                                                                             <  1:379083/128‑1 (MQ=255)
                                     aaTGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTTTTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAATATAA‑‑TTAGAATACTaaaa                                                                                            >  2:504992/1‑128 (MQ=255)
                                      aTGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAAATATAA‑‑TTTGCATAATATaa                                                                                            >  1:4902563/1‑128 (MQ=255)
                                      aTGCTGAACCAAGTACAATGCTAAGTGAAGTGGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAATATAA‑‑TTTGCATACTaaaa                                                                                            >  2:2948084/1‑128 (MQ=255)
                                         cttaaCCAATTAAAATTCTAAGTGAATTTGATTAATAAAAAAATATTAAACCAAATACATTTTGTTTGGTTTTTTTATTTACTATT‑‑T‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATaaaaaa                                                                                        <  1:2770160/125‑1 (MQ=255)
                                         cTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATAATATaaaaa                                                                                         >  1:5232867/1‑128 (MQ=255)
                                            aaCCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAAttt                                                                                     >  1:1707509/1‑127 (MQ=255)
                                             aCCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAAAATAA‑‑TTTTCATAAATTAAAAAAtt                                                                                      >  1:714890/1‑128 (MQ=255)
                                               cAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTTCATAATAAAAAAAATtttt                                                                                   >  1:1626628/1‑128 (MQ=255)
                                                aaGTACAATGCTAAGTGATGTTTTTTAATAATAAAAGATTAAACAAAAAACATTGTGTTTGGTTTTTTTATTTAATATT‑‑‑‑‑TAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGttttt                                                                                <  1:3110555/128‑1 (MQ=255)
                                                aaGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGttt                                                                                  >  1:4462247/1‑128 (MQ=255)
                                                aaGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAAAATAA‑‑TTTAAAAACTATAAAAAAtatatt                                                                                  >  1:2160258/1‑123 (MQ=255)
                                                  gTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATAAAA‑‑TTTAAATAAAAAAAAAAAtttttttt                                                                                >  2:5192742/1‑128 (MQ=255)
                                                  gTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAATATAA‑‑TTTGAAAACTATAAAAAAATGtatta                                                                                >  2:3391780/1‑127 (MQ=255)
                                                  gTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAATAATATAA‑‑TTTGCATAATATAAAAAAttttttttaa                                                                              >  2:1085669/1‑126 (MQ=255)
                                                   taaaaTGTTAAGTTTATTTTATTAAAAATAAAATATTAAAACAAATAAATTGTGTTTGTTTTTTTTATTTAATTTTTAA‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGttttt                                                                                <  2:4541557/125‑1 (MQ=255)
                                                      aaTGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAAAAAAA‑‑TATAAATAATATAAAAAATTGTTTTTCaa                                                                             >  1:4265203/1‑128 (MQ=255)
                                                       aTGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAATAAAA‑‑TTTAAAAAAAAAAAAAAATTTTTTTTaaaaa                                                                           >  1:2642862/1‑127 (MQ=255)
                                                       aTGCTAAGTCAATTTGATTAATAATAAAATATTAAACCAAATAAATTGTGTTTGGTTTTTTTTTTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAAt                                                                           <  1:2996952/128‑1 (MQ=255)
                                                        tGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAATATAA‑‑TTTGAATAATATAAAAAAATTTTTTTAAAATaa                                                                         >  1:460760/1‑128 (MQ=255)
                                                          ctttgTGAAGTTGTTTAATAATATATTATTAAAATAATTAAATTTTGTTTTGTTTTTTTATTTAATTTTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAg                                                                        <  2:418808/124‑1 (MQ=255)
                                                          cTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGAATAATATAAAAAATTTTTTTTAAATTaat                                                                        >  2:4855906/1‑127 (MQ=255)
                                                               ttttttttattaataatAAAATTTTAAAAAAATTATTTTTTTTTTGTTTTTTTTATTTATTTTTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTc                                                                   <  2:2503924/117‑1 (MQ=255)
                                                               tGAATTTGATTAATAATAAAAGAATAAACAAAAAACATTGTGTTTGTTTTTTTTATTTATAATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTc                                                                   <  1:1817196/128‑1 (MQ=255)
                                                                   gTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAATATAA‑‑TTTCCATACTAAAAAAAATTTTTTTACAAATAAGTACTCAgaa                                                               >  2:4970663/1‑126 (MQ=255)
                                                                    ttGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTTAAAAAAATAAAAAATTTTTTTTAAATTAattattaattat                                                              >  2:4300600/1‑122 (MQ=255)
                                                                    ttGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTTCATAATATAAAAAATTGTTTTTAAAATAATTATTAAgtatt                                                             >  1:120139/1‑124 (MQ=255)
                                                                      ttttaataaaAAAATATTAAACCAAATAATTTTGTTTTTGTTTTTTTTTTTAAATTT‑‑T‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTaa                                                           <  2:2859132/124‑1 (MQ=255)
                                                                      gatgaATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTTAATAATATAAAAAATTGTTTTTCAAATAATTATTAAgtatta                                                            >  1:4407597/1‑123 (MQ=255)
                                                                       atgaATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAAAAAAAAAA‑‑TTTAAATAATAAAAAAAATTTTTTTTAAAAAAATTAtaaagaaata                                                            >  2:2133969/1‑119 (MQ=255)
                                                                        tgaATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAATTATTAATGAGTaaa                                                          >  1:1954105/1‑128 (MQ=255)
                                                                        tgaATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTACATAATATAAAAAATTTTATTTAAAAAAAGTATTAAGTATAAAtt                                                        >  2:2782322/1‑128 (MQ=255)
                                                                          aataatAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATATTATAA‑‑TTTGATAACTATAAAAAATAGTTTTAAAAATAAGTATTCAGGAgatatttt                                                       >  1:3865984/1‑121 (MQ=255)
                                                                                  aaGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAATATAA‑‑TTTCAATAATATAAAAAATTATTATAAAAATAATAATAAAGAATAAAATTTaaaaaa                                                 >  1:5366286/1‑128 (MQ=255)
                                                                                   aGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTTAATAATATAAAAAATTTTTTTTAAAATAATTATTAATGATTAAATTTAAAAAAca                                               >  1:3337697/1‑127 (MQ=255)
                                                                                     aTTAAATAAAATTTATTTTTTTTGGTTTTTTTATTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCaa                                             <  1:3992709/128‑1 (MQ=255)
                                                                                     aTTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAAAAAAAAAA‑‑TTTAAAAAAAAAAAAAAATTTTTTTTAAAATAATTATTAATAAAAAAATTAAAAAAACaaa                                             >  2:3719766/1‑128 (MQ=255)
                                                                                      ttAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAT‑‑TTTGAATAATATAAAAAATTTTTTTTAAAAAATTTATTCAGTAGTAAATTTAAAAAACaaaa                                            >  1:1523950/1‑127 (MQ=255)
                                                                                      ttAAACCAAATAAATTTTGTTTGGTTTTTTTATTTAATATT‑‑T‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAaga                                           <  1:2241351/128‑1 (MQ=255)
                                                                                        aaaaaaaaTATATTGTTTTTGTTTTTTTTTTTTAATATT‑‑T‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGt                                         <  2:1465961/123‑1 (MQ=255)
                                                                                         aaCCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATAAAA‑‑TTTGAATAATATAAAAAATTTTTTTTAAAATAATTATAAATGATTAAATTTAAAAAACaaataat                                         >  2:4768587/1‑124 (MQ=255)
                                                                                         aaCAAAAAAAATTTTATTTGGTTTTTTTATTTAATTTTTAA‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAagag                                          <  2:5409682/128‑1 (MQ=255)
                                                                                           ccAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGAATACTATAAAAAATTTTTTTTAAAATAATTATTAATTATTAAATTTAAAAAACaaatatatat                                      >  1:97114/1‑121 (MQ=255)
                                                                                            cAATTACATTTTGTTTTGTTTTTTTATTTAATAAT‑‑T‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtata                                     <  2:199563/128‑1 (MQ=255)
                                                                                            cAAATAATTTTTTTTTGGTTTTTTTATTTAATATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCTAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGATTtat                                      <  1:128629/128‑1 (MQ=255)
                                                                                            cAAAAAAATTGTGTTTGGTTTTTTTTTTTAATTAT‑‑T‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtata                                     <  2:1187845/128‑1 (MQ=255)
                                                                                             aattACTTTTTGTTTTGTTTTTTTAATTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtata                                     <  2:1856423/125‑1 (MQ=255)
                                                                                             aaaTACATTTTTTTTGTTTTTTTTTTTTAAATTTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTtata                                     <  2:1330166/128‑1 (MQ=255)
                                                                                             aaaTACATTGTTTTTGTTTTTTTTATTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TATGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGATTtata                                     <  1:256076/128‑1 (MQ=255)
                                                                                             aaaTACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACAATAAAAAATTGTTTTTAAAAAAAGTATTAAGGATTAAATTTAAAAAAACAagctgtata                                     >  2:3063333/1‑121 (MQ=255)
                                                                                             aaaTACATTGTGTTTGGTTTTTTTATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGAATAATATAAAAAATTTTTTTTAAAATAATTATTCAGTATTAAATTTAAAAAACAAATAGTaata                                     >  1:5447582/1‑128 (MQ=255)
                                                                                             aaaTACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGAAAAATATAAAAAATTGTTTTTAAAATAATTATTAATTATTAAATTAAAAAAACCaaagaacgtaa                                    >  1:2834624/1‑119 (MQ=255)
                                                                                               aTACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAAAATAA‑‑TTTGCAAAATATAAAAAATTTTTTTTAAAATAATTATTAATAATTAAATTTAAAAAAACAAAAGTTATAAAt                                  >  2:1653179/1‑128 (MQ=255)
                                                                                               aTACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAATAATAAATTTTGAATAATATAAAAAATTTTTTTAAAAATAATTATTAAGTATTAAATTTAAAAAACAAATAGTtata                                     >  1:1122785/1‑128 (MQ=255)
                                                                                                taaaTTGTTTTTGTTTTTTTTATTTAAAATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAAt                                  <  2:2618015/125‑1 (MQ=255)
                                                                                                tACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAAAAATATAT‑‑TTGGAATAATTTAAAAAATTTTTTTAAAAAAAATTATTATTAATAAAATTAAAAAAACAAATATTTATaataa                                 >  1:896725/1‑125 (MQ=255)
                                                                                                caCAATGTATTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAAATAAAATAA‑‑TTTGAATAATATAAAAAATTTTTTTTAAAATAATTAATAATAATTAAATTTAAAAAACAAATATTTATaa                                    >  1:1000173/2‑128 (MQ=255)
                                                                                                aaaaatttgttttGGTTTTTTTATTTAAAATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATaaa                                   <  2:4012811/118‑1 (MQ=255)
                                                                                                  taattttttttGTTTTTTTTATTTAATATT‑‑T‑‑‑AAAAAATAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCt                                <  2:856355/120‑1 (MQ=255)
                                                                                                   tttgtttttgtttTTTTTATTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCtt                               <  2:178073/127‑1 (MQ=255)
                                                                                                   aatgtaTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAATATAA‑‑TTTGAATACTATAAAAAATTGTTTTTAAAATAATTATTAAGGAGTAAATTTAAAAAACAAATAGTTATaaaaat                                >  2:693515/3‑125 (MQ=255)
                                                                                                   aTTGTGTTTGGTTTTTTTATTTACTATT‑‑GAAAAAAAAAAAAAAAAATAATATAA‑‑TTTGCAAACTATAAAAAATTTTTTTTAAATTAATTATTAATTAGTAAATTTAAAAAAAAAATATTTATAAATCt                                >  1:3671574/1‑128 (MQ=255)
                                                                                                    ttGTGTTTTGTTTTTTAATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTg                              <  2:3836868/128‑1 (MQ=255)
                                                                                                       tgtTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTTGtttt                         >  1:5163264/1‑128 (MQ=255)
                                                                                                          tttGTTTTTTTTATTTACTATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGtttt                         <  1:2356229/128‑1 (MQ=255)
                                                                                                            tGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGAATACTATAAAAAATTTTTTTTCAAATAATTATTAATTATTAAATTTAAAAAACAAAAATTTAAAATACTTGGttttatt                      >  2:3494385/1‑125 (MQ=255)
                                                                                                               tttttttATTTACTATT‑‑G‑‑‑AAAAAAAATATAAAATAATATAA‑‑TTTGTATACTATAAAAAATTGTTTTTCAAATAAGTATTGAGGAGTATATTTAATTAACCAAGAGTTATAAATCTTGGTTTTTTGttt                   <  2:5502533/128‑1 (MQ=255)
                                                                                                               tttttttATTATTATT‑‑G‑‑‑AAATAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATTAAAAATTGTTTTTCAAATAAGTATTCTGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGttt                   <  1:3573388/128‑1 (MQ=255)
                                                                                                                  ttttATTTACTATT‑‑GA‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGATTAATTTTAAAAAACAAAGATTTATAAATATTGGTTTTTTGTTtat                 >  1:4710784/1‑128 (MQ=255)
                                                                                                                  ttttATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAT‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTAAGGAGTAAATTTAAAAAACCAAGAGTTATAAAAATTGGTTTTTTGTTtatt                >  1:4953782/1‑127 (MQ=255)
                                                                                                                  ttttATTTAATATTT‑‑A‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTtat                 <  1:4333183/128‑1 (MQ=255)
                                                                                                                  ttttATTTAATATT‑‑T‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTtata                <  1:2060153/128‑1 (MQ=255)
                                                                                                                   tttatttaTTTTT‑‑G‑‑‑AAAAAAAAATAAAAAATAATATAA‑‑TTTGAATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTtata                <  2:2329344/128‑1 (MQ=255)
                                                                                                                   tttatttaTTATT‑‑T‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTatat               <  2:3196658/128‑1 (MQ=255)
                                                                                                                   tttatttaCTATT‑‑GAAAAAAAAAAAAAAAAAAAAATATAA‑‑TTTGAATAATTTAAAAATTTTTTTTTAAAAAAATTATTAAGGAGTAATTTTAAAAAACAAAGATTTTTAAATATTGGTTTTTGTTtat                 >  1:405990/1‑128 (MQ=255)
                                                                                                                    ttatttaCTATT‑‑GAAAAAAAAAAAAAAAAAAAATATAT‑‑TTTGAATAATATAAAAATTTTTTTTTAAAAAAATTATAAAGTTTAAAATTTAAAAAACAAATATTTAAAAAATTTGTTTTTTTTTTtatt                >  1:4843686/1‑127 (MQ=255)
                                                                                                                    ttatttaCTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAAAAAAA‑‑TTTAAAAAATATAAAAAATTTTTTTTAAAAAAATAATTAAATATTAAATTAAAAAAAAAAAAATATATAAATATTTTTTTTTTTTTTAtttt              >  2:2229860/1‑128 (MQ=255)
                                                                                                                    ttatttaATATT‑‑T‑‑‑AAATAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTCGTTTTTTGTTTatat               <  2:1536850/128‑1 (MQ=255)
                                                                                                                        ttaCTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAT‑‑TTTGCATAATATAAAAAATTGTTTTTAAAATAATTATTCATGATTAAATTTAAAAAACAAAGATTTATAAATATTGGTTTTTTTTTTAtattatta          >  1:5532267/1‑128 (MQ=255)
                                                                                                                              aaTT‑‑A‑‑‑AAAAATAAAAAAAAATAATATTA‑‑TTTGAATACTTTAATAAATTGTTTTTCATATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTATTTg      <  1:14182/127‑1 (MQ=255)
                                                                                                                               aTT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGATTAAATTTAAAAAACCAAGATTTATAAATATTGTTTTTTTTTTTATATTTTTAttttt     >  2:4622417/1‑126 (MQ=255)
                                                                                                                                  t‑‑G‑‑‑AAAAAAAAAAAAAAAAAATATAA‑‑TTTGCATACTTTAAAAAATTGTTTTTAAAATAATTATTCAGGATTAAATTAAAAAAACCAAGATTTAAAAAAATTGTTTTTTTTTTTTTATTATTATTTGtgt   >  1:4542520/1‑126 (MQ=255)
                                                                                                                                      |                                                                                                                                        
CTTTACTTCTTTATGAGTTTCAACAAAAAGAGTAAGAAATGCTGAACCAAGTACAATGCTAAGTGAAGTTGATGAATAATAAAAGATTAAACCAAATACATTGTGTTTGGTTTTTTTATTTACTATT‑‑G‑‑‑AAAAAAAAAAAAAAATAATATAA‑‑TTTGCATACTATAAAAAATTGTTTTTCAAATAAGTATTCAGGAGTAAATTTAAAAAACCAAGAGTTATAAATCTTGGTTTTTTGTTTATATTATTATTTGTCTT  >  CP016816/34818‑35072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: