Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2510T.53.0% inf / inf 1718intergenic (+215/‑327)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (395/334);  new base . (641/182);  total (1052/666)
Fisher's exact test for biased strand distribution p-value = 4.08e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2144 total aligned reads displayed.

TAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTT  >  CP016816/165124‑165379
                                                                                                                                |                                                                                                                                 
 aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttt                                                                                                                                  >  1:1203203/1‑128 (MQ=255)
  atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttt                                                                                                                                 >  1:2554399/1‑128 (MQ=255)
  atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttt                                                                                                                                 >  2:3912684/1‑128 (MQ=255)
    atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAAATTGAACTTGTAAAAAATAGCtttttt                                                                                                                               >  1:3535186/1‑128 (MQ=255)
       ttttttCATTAATTTATCGCACTTTTTTTAATTATTAATAAACTAAAAATATTATATTTTTTTTTATTTTTTTTAAATTCAAAATTTTAAAAAATTAATTTTAAATTTTAAAAAATATCttttttttt                                                                                                                            <  2:801611/125‑1 (MQ=255)
       tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCttttttttt                                                                                                                            >  1:1813343/1‑128 (MQ=255)
        attttAATTTATTTATCTAAAATGTATTAATTATTAATAAAATAAAACTTTTATATTTTTAGATATTTTTTGTATATTTAAATTTTTAAAAAATTTATTTTAAATTTTAAAAAATATCtttttttttt                                                                                                                           <  2:5429439/126‑1 (MQ=255)
            tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttt                                                                                                                       >  1:3892295/1‑128 (MQ=255)
              aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttt                                                                                                                     >  1:1767538/1‑128 (MQ=255)
              aTTAATTTATATCAATTTTTTTTATTATTAATAAAATAAAAATAATTTATTTTTTTTTTTTTTTTTTATTTTCAAATTTTTTAAAAATTAATTTTAACTTTTTAAAAAAACCtttttttttttttttt                                                                                                                     <  1:2161777/128‑1 (MQ=255)
               ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTATTTTTAACTTGTAAAAAATATAttttttttttttttttt                                                                                                                    >  2:1121618/1‑128 (MQ=255)
               ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttta                                                                                                                    >  1:2648123/1‑127 (MQ=255)
                 aaTTTAACGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATATTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAttttattatttattttaaa                                                                                                                  >  1:3142691/1‑125 (MQ=255)
                   tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttatttttataat                                                                                                                >  1:4524282/1‑123 (MQ=255)
                    ttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttaaaatg                                                                                                               >  2:2541436/1‑122 (MQ=255)
                      ttCGCATTTTTATGTATTATTAAGAAAATAAAAATATTATCTTTTTATATATTTTTTGTTTATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATATCTTTTTTTTTTTTTTTTtataatgg                                                                                                             <  2:247665/127‑5 (MQ=255)
                      atagCAATTGTATGTATTAGTAATAAACTAAAACTATTATCTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAAATAATTTGAAATTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataatgg                                                                                                             <  1:5225097/125‑5 (MQ=255)
                      aTCTCAATTGTATGAATTTGTAAGAAACTAAAACTATTATTTTTTTATATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCttttttttttttttttataatgga                                                                                                             <  1:1901547/128‑9 (MQ=255)
                      aTCGCACTTGATTGAACTAGTAAGAAACTAAAACTATTATCTTTTTAGAAATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttataacgga                                                                                                             <  1:2348421/128‑9 (MQ=255)
                        cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTTATTTGAACTTGTAAAAAATAGCttttttttttttttttttatatatta                                                                                                           >  2:3808806/1‑120 (MQ=255)
                        cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataatttaa                                                                                                           >  1:3782613/1‑122 (MQ=255)
                         gCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttattatttaaa                                                                                                          >  1:5481520/1‑118 (MQ=255)
                          cacaTGTATTAATTATTAATAAACTAAAACTATTAACTTTTTATATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAat                                                                                                       <  1:4947199/128‑1 (MQ=255)
                            cTTGTTTTAATTATTAATTAAATAAAACTATTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAAATTTAACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                                                    <  1:3044296/128‑1 (MQ=255)
                            cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttttatttgaaaaa                                                                                                       >  2:554838/1‑117 (MQ=255)
                            cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTtaaatttaaaaa                                                                                                       >  2:2375188/1‑118 (MQ=255)
                            cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGACATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTaatttaaatata                                                                                                       >  2:1036147/1‑119 (MQ=255)
                             ttGTATGAATTAGTAGGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATTAAAAtata                                                                                                    >  1:5112545/1‑128 (MQ=255)
                             ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAAtata                                                                                                    >  1:1106049/1‑128 (MQ=255)
                             ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAttaaa                                                                                                    >  2:3415668/1‑126 (MQ=255)
                             atttatttattattAACAAAATAAATATTTAATCTTTTTTTTTATTTTTTTTATTTTGAAATTTTTAAAAAATATATTTGAAATTGTAAAAAAATCCTTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                                                    <  2:2136820/120‑1 (MQ=255)
                              tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataataaaaattaaa                                                                                                     >  1:2447072/1‑116 (MQ=255)
                                tttGAATTATTAATAAAATAAAAATATTATATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                <  1:27399/126‑1 (MQ=255)
                                tatttattattAATAAACTAAAAATATTATCTTTTTAGATATTTTTTTTTTATTCAAATTTTTAAAAAATTAATTTGTACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                <  2:1936335/123‑1 (MQ=255)
                                tatttattattAATAAAATAAAAATTTTATTTTTTTTTATTTTTTTTTTATATGCAAATTTTTTAAAAATTAATTTTAACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                <  2:184477/123‑1 (MQ=255)
                                tatttattattAATAAAATAAAAATTTTATTTTTTTATATATTTTTTTTTTATTAAAATTTTTAAAAAATTTATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTTATAATGGAAAtata                                                                                                    <  2:1435609/123‑1 (MQ=255)
                                tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTTTAAAAATAAC‑‑CTTTTTTTTTTTTTTTTATATTTGAAATATAAAtt                                                                                                >  2:2956906/1‑128 (MQ=255)
                                 aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAAt                                                                                                 >  1:197568/1‑128 (MQ=255)
                                 aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTAAAAAAAAAAt                                                                                                 >  1:4749201/1‑128 (MQ=255)
                                  tgcattattAATAAAAAAAAACATTTTTATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTTAAAAATATCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                                <  2:4418882/125‑1 (MQ=255)
                                    aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAAATATAAAttt                                                                                               >  1:3245174/1‑128 (MQ=255)
                                     aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTat                                                                                            >  1:4331656/1‑127 (MQ=255)
                                       tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAATTATAAATTTTAgtt                                                                                          >  1:561704/1‑126 (MQ=255)
                                        aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATTTTAATTTTATGTa                                                                                         >  2:5312863/1‑128 (MQ=255)
                                         ttaataaACAAAAAATATTATTTTTTTTTATATTTTTTTTTTATGAAAATTTTTAAAAAAATAATTTTAAATTTTAAAAAAAAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                                                        <  1:5395137/127‑1 (MQ=255)
                                          tAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTATAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATTTAAAttttttttatt                                                                                       >  1:961484/1‑121 (MQ=255)
                                           aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTAttt                                                                                      >  1:5036559/1‑128 (MQ=255)
                                             gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAAATTTAAAAATAAATTTTATATAttt                                                                                      >  1:4098172/1‑128 (MQ=255)
                                              aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAAAATAAAATTTAGTTAtttttt                                                                                   >  2:4716249/1‑126 (MQ=255)
                                              aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTAAAATGTAATAATAAATTTTAGTTAtttttt                                                                                   >  1:2943245/1‑126 (MQ=255)
                                                actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATTATTTAAATATAAATTTTAGTTATtttttt                                                                                  >  2:3542043/1‑128 (MQ=255)
                                                 ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTAGGTATtttttttt                                                                                >  2:1742693/1‑126 (MQ=255)
                                                      actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAAAATATATTTTAATTATttttttttaaaat                                                                           >  2:640455/1‑121 (MQ=255)
                                                        tattatATTTTTTGATATTTTTTGTATATTCAAATTTTTAAAAAATTTATTTGAACTTGTAAAAAATA‑‑‑‑‑TGTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                                                      <  2:5199656/128‑1 (MQ=255)
                                                         attatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTTAGGTATTTTGTTTTATAATTaaa                                                                       >  2:3047494/1‑126 (MQ=255)
                                                            atCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATTTATTTTGTTTTATaatattata                                                                     >  1:2846611/1‑121 (MQ=255)
                                                               tttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAAAATAATATTTAATTTTTTTTTTTTTTAATTTTCTaaa                                                                   >  2:3673809/1‑128 (MQ=255)
                                                                 tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAATAATAGC‑‑TTTTTTTTTTTTTTTTAAAAATTAAATAAAAAATTTTAGATATTTTTTTTTATAATTATaaaaaaaa                                                                >  1:2067210/1‑128 (MQ=255)
                                                                   tttatatTTTTTTTATTTTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATTGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                                                               <  1:4679550/125‑1 (MQ=255)
                                                                    ataaatTTTTATTATATTCAAATTTTTAAAAAATTAAATTTAAATTTTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATa                                                             <  1:3626999/124‑1 (MQ=255)
                                                                     ttttttttttttATATTTAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                                                            <  2:2008358/124‑1 (MQ=255)
                                                                     gATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑‑TTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAg                                                        <  1:654953/128‑1 (MQ=255)
                                                                       tatTTTTTTTTTATCCAAATTTTTAAAAAATTAATTTTAACTTTTTAAAAAT‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGaaa                                                         <  2:2831197/128‑1 (MQ=255)
                                                                           ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTCTAATTATCTAAAAAAATATaaatg                                                       >  1:878812/1‑126 (MQ=255)
                                                                            ttttTATATTAAAATTTTTAAAAAAATAATTTTTATTTTTTAAAAATAGTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGa                                                      <  2:2240571/128‑1 (MQ=255)
                                                                             ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTTTTTTGTTGTATAATTATATAAAAAAATATAATGGaaa                                                    >  1:442782/1‑128 (MQ=255)
                                                                              tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATATTTTAAATATAAATTTTAGGTTTTTTGTTTTAAAATTTTAAAAAAAAATATAAAGaaaa                                                    >  2:876227/1‑128 (MQ=255)
                                                                              tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATTTTTTTTTTTAATTATATAAAAAAATATAAAGTaaaa                                                   >  1:1539467/1‑128 (MQ=255)
                                                                               gTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATTTAAATATAAATTTTTGTTATTTTTTTTTAAAATTATATAAAAAAATAAAAATTAAAAAata                                              >  1:70040/1‑128 (MQ=255)
                                                                                 atatGTAAATTTTTAAAAAATTAATTTTTACTTGTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata                                              <  1:1291834/128‑1 (MQ=255)
                                                                                 atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGGAAATATAAATTTTAGGTATTTTGTTTTATAATTATATAAAAAAATAAAAAGGAAAAAat                                               >  2:4068909/1‑128 (MQ=255)
                                                                                  tatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATTTAATTTTTAGGTTTTTTGTTGTATAAGTATCTAAAAAAAAAGAAAGaaataaattt                                             >  2:4494316/1‑126 (MQ=255)
                                                                                    tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTTTATAATTATATAAAAAAATAGAAAGGAAAAAATAtttt                                          >  2:2686122/1‑126 (MQ=255)
                                                                                       aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGTTATTTTTTTGTCTAATTATATAAAAAAATAGAAATGAAAAAATATTGtt                                         >  2:5078314/1‑128 (MQ=255)
                                                                                        aaTTTTTAAAAAATTAATTTGAACTTTTAAAAAA‑‑‑‑TTTCTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaaa                                      <  2:1619416/128‑1 (MQ=255)
                                                                                         aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTAATTTAAATAAAAATTTTATTTTTTTTTTTTTATAATTATAAAAAAAAAAAAAAAATAAAAAAAATTTTTa                                        >  1:4609954/1‑128 (MQ=255)
                                                                                         aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAAATTTATGTATTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAATATTTTTa                                        >  2:3260730/1‑128 (MQ=255)
                                                                                             ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTGTAAATAAAAATTTTATGTATTTTGTTTTATAAGTATATAAAAAAATAGAAAGTAAAAAATATTTTTaaaataa                                  >  1:3325149/3‑125 (MQ=255)
                                                                                              taaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTT‑‑‑TAATGGAAATATAAATTTTAGGTTTTTTGTTGTCTAAGTATATAAAAAAATATAAAGGAAAAAATATTGTTAAAATTat                                 >  2:4272393/2‑127 (MQ=255)
                                                                                                 aaaattaattTTAAATTTTAAAAAAT‑‑‑TTCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATAc                              <  1:4839880/126‑1 (MQ=255)
                                                                                                    attaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAAATGGAAATATAAATTTTTGTTAATTTTTTGTATATTTATCTAAAAAAATATAATTTAAAAATATATTTTAAATTTAATaat                             >  1:3723854/1‑126 (MQ=255)
                                                                                                    attaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTATTGTAAATTTAAATTTTAGGTATTTTGTTGTCAAAGTATCTAAAAAAAAAGAAATGTAAAAATATTGTTAAAATAAATACt                             >  2:4998429/1‑128 (MQ=255)
                                                                                                    attaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTTTTTTTTTTTAAATTTATATAAAAAAATTTAAATTAAAAATTTTTTTTAAATTTAATACt                             >  1:226052/1‑128 (MQ=255)
                                                                                                    attaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTTTATAATTATATAAAAAAATATAATGGAAAAAATATTTTTAAAAataataat                             >  2:4897950/1‑126 (MQ=255)
                                                                                                    attaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAAATGAAAATTAAAATTTTATGTATTTTTTTTTAAAATTATAAAAAAAAATAAAATGTAAAAAATATTTTAAAAAataataaa                             >  2:4179595/1‑126 (MQ=255)
                                                                                                     ttaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAATTTTTAGGTATTTTTTTTTATAATTATATAAAAAAATATAAAGGAAAAAATATTGTTAAAAGtaataataa                           >  1:1379792/1‑128 (MQ=255)
                                                                                                     ttaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAATTATAAATTTTATGTTTTTTGTTGTAAAATTTTATAAAAAAATATAAAGAAAAAAAAATTTTTAAAATTAAAAATaa                           >  1:4003404/1‑128 (MQ=255)
                                                                                                     ttaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTATTTTTTTTTATAATTATATAAAAAAATTTAAAGGAAAAAATATTTTTAAAATtaataataa                           >  1:796708/1‑128 (MQ=255)
                                                                                                        attTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAATTTTTAGGTTTTTTTTTGTATAAGTTTCTAAAAAATTATAAAGTAAAAATTTTTTTTAAAATTAATAAAAAAat                        >  1:4161060/1‑128 (MQ=255)
                                                                                                               cTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAT‑‑TGGATATATAAATTTTTGGTTTTTTTTTTTATAAGTATAAAAAAAATTAAAATGTAAAAAAAAATTTTATAATTAATAATAAAatttaatat                 >  2:1253612/1‑122 (MQ=255)
                                                                                                                 tGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAT‑‑TGGAATTTTAATTTTTTGTTTTTTTGTTTAAAAATTATATAAAAAAATAAAAAGAAAAAATTATTTTAAAAATTAATAAAAAAattattaattt               >  1:4241566/1‑123 (MQ=255)
                                                                                                                  gTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTGTCTAAGTATATAAAAAAATATAAAGGAAAAAATATTTTTAAAATTAATACTAAAATTTTTTAATGtt             >  2:134267/1‑128 (MQ=255)
                                                                                                                   ttttttaaaaattttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAAt                <  2:4587427/116‑1 (MQ=255)
                                                                                                                   tttaaaaaattttttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAAt                <  2:3151432/116‑1 (MQ=255)
                                                                                                                   tAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAATTATAAATTTTAGGTTTTTTGTTTTATAATTTTATAAAAAATTATAAATTAAAAAATATTTTTAAAATTAATAATAAAATTATTTAAtttt             >  1:101061/1‑128 (MQ=255)
                                                                                                                       taaatttttttttttttttttttttttATAATGGAAATATAAATTTTATGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAATGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGtta            <  1:341773/120‑1 (MQ=255)
                                                                                                                        aaTAGCTTTTTTTTTTTTTTTTTTTTTTAATGGAAATTTAAATTTTTGGTTTTTTTTTTTATTTTTATATAAAAAAATTTAAATTAAAAAATATTGTTAAATTTAATAATAAAATTATTTAATTttaa           >  1:5302556/1‑128 (MQ=255)
                                                                                                                           tatatttttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta        <  1:1629777/124‑1 (MQ=255)
                                                                                                                              gcttttttttttttttttttATAATGGAAATAAAAATTTTAGGTATTTTGTTGTAAAATTATATAAAAAAATAGAAAGGAAAAAATATTTTTAAAAGAAATAAAAAAATTATTTAATGTTAATTaaaa     >  2:4800989/3‑128 (MQ=255)
                                                                                                                               attttttttttttttttttATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAAc    <  2:1535376/127‑1 (MQ=255)
                                                                                                                                |                                                                                                                                 
TAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAACTT  >  CP016816/165124‑165379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: