Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4810CA38.8% 464.2 / 111.7 508intergenic (+259/‑416)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (138/148);  new base A (49/132);  total (200/308)
Fisher's exact test for biased strand distribution p-value = 4.99e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 593 total aligned reads displayed.

AGTGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATCCCCTTTTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATA  >  CP016816/202354‑202604
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agtGAAGTTAGTGTAAATAATAGAGTTAAGTTTAATTAAAATAAAATAAACTTAATAATTATTATAATTTATATCATCTATATCTATTTTTTTATTTTATTATCTTC‑ACAAAA‑AAAAAAAAAACAATc                                                                                                                               >  1:3936585/1‑128 (MQ=255)
agtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATc                                                                                                                               <  1:3683220/128‑1 (MQ=255)
agtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATc                                                                                                                               <  1:4351576/128‑1 (MQ=255)
agtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATc                                                                                                                               >  2:4536405/1‑128 (MQ=255)
 gtGAATTTAGTGTTAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAAATATTCTTATAATGAAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATcc                                                                                                                              <  1:648468/128‑1 (MQ=255)
 gtGAAGTTTTTGTATATAATATTGTTTATGTTAATTAATATATAATTAATGTAATATTTCTTTTTATGTATATAATCGGGTTCTATTTTTTTATTTGTTTTTCTTC‑ACAAATAAAAAAAAAAACAATc                                                                                                                               <  2:3450739/128‑1 (MQ=255)
 gtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATcc                                                                                                                              <  2:5175515/128‑1 (MQ=255)
 gtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATcc                                                                                                                              <  1:2845297/128‑1 (MQ=255)
 gtGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGATTATCTTC‑ACAAAT‑‑AAAAAAAAACAATccc                                                                                                                             >  2:678912/1‑128 (MQ=255)
   gAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATcccc                                                                                                                            <  1:4307316/128‑1 (MQ=255)
    aaGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATcaact                                                                                                                           >  1:2702144/1‑124 (MQ=255)
      gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTTATCTTC‑AAAAAT‑AAAAAAAAAACAAACACCtta                                                                                                                         >  2:2510582/1‑127 (MQ=255)
      gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATc                                                                                                                               <  2:2153908/122‑1 (MQ=255)
      gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCAAAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCAATTTTAATATTTCTATATCTTC‑ACAAAT‑AAAAAAAAAAAAAACACCtta                                                                                                                         >  2:1325743/1‑127 (MQ=255)
       ttAGTTTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTAAATAATTCTTATAATGTAAATCATCGTTAACTATATTTTTATTTATATAAATTA‑ACAAAT‑AAAAAAAAAAAAAAACCCttaa                                                                                                                        >  2:1901925/1‑126 (MQ=255)
       ttAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTAAAAATTTATATAATCTATATAAATTTTTTAATTTTTAAAAATTC‑AAAAAA‑AAAAAAAAAAAAATcaaaaaaa                                                                                                                        >  1:5408964/1‑121 (MQ=255)
         aGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCATATAATGTAGATCCTCGTGATCTATTTATTTATTAGTTTATCTTC‑ACTAAT‑AAAAAAAAAACAATcaaataaaaa                                                                                                                      >  1:4690961/1‑119 (MQ=255)
           tgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTTTTTATCTTA‑ACAAAA‑AAAAAAAAAAAAATaccaataaaaat                                                                                                                    >  2:5413571/1‑119 (MQ=255)
               aataatAGAGTTAAGGTTAATTAAAATACAATAAACTTAATAATTCTTATAATTTAGATCCTCGTGATCTATTTTTTTATTAGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATCCACtaaaaaattaaa                                                                                                                >  1:929422/1‑117 (MQ=255)
                 taataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATCCAC‑‑ttaaa‑‑aattaaaaa                                                                                                          >  2:4781775/1‑128 (MQ=255)
                   ataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTTATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATAAAC‑‑TTAAA‑‑AATTaaaaaaa                                                                                                        >  1:4734993/1‑128 (MQ=255)
                    taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATACC‑‑ATTAAA‑‑AATTaaaaataa                                                                                                       >  2:4250496/1‑128 (MQ=255)
                      gagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATATCCTCTTGATCTATTTTTTTATTTGTTTATCTTC‑AAAAAT‑AAAAAAAAAACAATAAC‑‑ATTAAA‑‑AATTaaaaaaaaaa                                                                                                     >  1:2066083/1‑128 (MQ=255)
                        tttAATGTTAATTAAAATAAAATAAAAATAATAATTATTATAATTTATTTACTTGTTTTTTATTTTTTTTTTTTTTTAAATTA‑AAAAAA‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaa                                                                                                   <  1:5025406/127‑1 (MQ=255)
                        gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑AAAAAT‑AAAAAAAAAACAATACAC‑‑TTAAA‑‑AATTaaaaaaaaaaaa                                                                                                   >  1:4535694/1‑128 (MQ=255)
                        gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTAGTGATCTATTATATTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAAAAATCAAC‑‑TAAAA‑‑AAATaaaaaaaaaaaa                                                                                                   >  1:256548/1‑128 (MQ=255)
                         ttAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTATTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAAAACCC‑‑ATAAA‑‑AATTaaaaaaaaaaaaa                                                                                                  >  2:4661901/1‑128 (MQ=255)
                           aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAAAAATAAAC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaa                                                                                                >  1:5507143/1‑128 (MQ=255)
                           aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCAACGTGATCTAATTATATATATATTAAAATTC‑ACAAAT‑AAAAAAAAAACAAACAC‑‑‑AAAAA‑‑AAATaaaaaaaaaaaaaaaa                                                                                               >  2:80064/1‑128 (MQ=255)
                           aaGGTTAATTAAAAAACCATAAACTTAATAAATCTTATAATGTAGATCCTCGTGATCTATTATTTTATTTGTTTATCTAC‑ACAAAT‑AAAAAAAAAACAATCAAC‑‑TTAAA‑‑AATTaaaacaaaaaaaaaa                                                                                                >  2:576089/1‑128 (MQ=255)
                            aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATACTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaa                                                                                               >  2:2316913/1‑128 (MQ=255)
                             tttaaattaaATAACAATAAA‑TTAAAAATTCTTATAATTTATATCCAAGTTATATTTTTTTTTTATTTTTTATATTA‑AAAAAT‑AAAAAAAAAAAAATCCCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaaa                                                                                             <  1:1953028/124‑1 (MQ=255)
                             ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAAACAAAAC‑‑AATAAA‑‑AAATaaaaaaaaaaaaaaaaa                                                                                              >  2:2636124/1‑128 (MQ=255)
                               ttaattaaAATACAATAAACTTAATAATTCTTATAATGTAGATACTCGTGATCTATTTTTTTATTTGTTTATCTTA‑ACAAAT‑AAAAAAAAAACAATCCAC‑‑‑TAAA‑‑AAATtaaaaaaaaaaaaaaaaaaa                                                                                           >  1:5169416/1‑128 (MQ=255)
                                taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTAGTTTATCTTA‑ACAAAT‑AAAAAAAAAAAAAAAACCATAaaaa‑‑aaaaaaaaaaaaaaaaaaaaaa                                                                                             >  2:1209751/1‑128 (MQ=255)
                                 aattaaAATACCATAAACTTAATAATTATTATAATGTAGATACTCGTGATCTATTTTTTTATATTTTTATATTC‑ACAAAT‑AAAAAAAAAAAAATCAC‑‑AATAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaa                                                                                          >  1:3640552/1‑128 (MQ=255)
                                 aattaaAATACCATAAACTTAATAATTATTATAATGAAGATAATATTGATCTATTATTTTATTTAATTATATTC‑ACAAAA‑AAAAAAAAAAAAAAAAAC‑‑TTAAA‑‑AAATaaaaaaaaaaaaaaaaaaaaa                                                                                          >  1:1795945/1‑128 (MQ=255)
                                 aattaaAATACCATAAACTTAATAATACTTATAATGTAGATCCTCGTGATCTATTTATTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAAAAAACAACTTaaaaa‑‑aaaaaaaaaaaaaaaaaaaaaaa                                                                                            >  2:1144124/1‑128 (MQ=255)
                                  attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTTATCTATTTTTTTATTTTTTTATCTTC‑ACAAAT‑AAAAAAAAAACAAT‑‑ACAATTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaa                                                                                         >  1:3871012/1‑127 (MQ=255)
                                    taaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTATTATCTTC‑ACAAAT‑AAAAAAAAAACAAAAACCTAaaaaa‑‑aaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                         >  2:1590580/1‑127 (MQ=255)
                                     aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAAACCAC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                      >  2:397699/1‑124 (MQ=255)
                                     aaaaTAACTTAAAATTAATAATTATTTAAATTTAGAACCTTATTTTCTATTTTTTTATTTTTTTATAATC‑AAAAAA‑AAAAAAAAAAAAATCCCC‑‑TTAAA‑‑AAAT‑AAAAAAAAAAAAAAAAAAAACTaaa                                                                                     <  1:3429097/128‑1 (MQ=255)
                                      aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATCCC‑‑ATTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                     >  2:4415098/1‑123 (MQ=255)
                                      aaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTA‑ACAAAT‑AAAAAAAAAAAAAAACCC‑‑TTAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                     >  2:2036518/1‑123 (MQ=255)
                                      aaaTACCATAAACTTAATAATTCTTATAATGTAGAACCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATCCCC‑‑ATAAA‑‑AATTaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                     >  2:1710258/1‑123 (MQ=255)
                                       aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                    >  2:2939745/1‑128 (MQ=255)
                                       aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAATaaaaaaaaaaaaaa                                                                                    >  2:4119310/1‑128 (MQ=255)
                                         tacataaaaaataataaTTTTAATAATATACAAACTCCTGAACTTTTTTTTTATTTTTTTATATTA‑AAAAAA‑AAAAAAAAAAAAATACCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                 <  1:1076287/117‑1 (MQ=255)
                                         taaaaTAAACTTAATAATTTTTATAATTTTTATCCTCGTTATCATTTTTTTTATTTTTTTAAAATC‑AAAAAA‑AAAAAAAAAACAATCCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                 <  1:1922006/124‑1 (MQ=255)
                                          aacATAAAATTAAAAAATATTAAAATTTATATACTCTTTATCTATTTTTTTTTTTTTTTATCTTA‑AAAAAA‑AAAAAAAAAAAAAAACCC‑‑ATAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                 <  1:80064/126‑1 (MQ=255)
                                          aaaaTAAAATTAAAAATTATTATAATTTATATACTCGTTATCTATTTTTTTATTTTTTTATATTA‑ACAAAA‑AAAAAAAAAAAAATCCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                 <  1:4192698/125‑1 (MQ=255)
                                           ccATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAAAAATACCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAACAaaaaaaaaaa                                                                                >  2:3640120/1‑128 (MQ=255)
                                            cATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAA‑AAAaaacaaaa                                                                              >  1:4505327/1‑127 (MQ=255)
                                            aaTAAAATTAAAAATTATTATAAATTAAATACAAGTTATATATTTTTTTATTTTTTTATTTTA‑AAAAAA‑AAAAAAAAAAAAATACCC‑TTAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                <  1:4626939/127‑1 (MQ=255)
                                             aTAAACATAAAAAATTTTATAATTTATATCCATGTGATTTATTTTTTTTTTTGTTTATATTA‑AAAAAA‑AAAAAAAAAAAAATCCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAaaacaaac                                                                              <  1:4256050/128‑1 (MQ=255)
                                               aaaaTTAATAATTATAATAATTTATTACATTGTTATTAATTTTTTTATTTTTTTAAATTA‑AAAAAA‑AAAAAAAAAAAAAACCCCTT‑‑‑AA‑‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                          <  2:1466451/128‑1 (MQ=255)
                                               aaaCTTCATAATTCTTATAATGTTGTTCCTCGTGATATATTTTTTTATTTTTTTATCTTC‑CCAAAT‑AAAAAAAAAA‑AATCCCCTT‑‑‑AA‑‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAt                                                                         <  2:280044/128‑1 (MQ=255)
                                                       taatTATTATAAATTATATAATCTTTATATATTTTTTTATTTTTTTAAAATA‑AAAAAA‑AAAAAAAAAAAAAATCCC‑CTTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCt                                                                     <  1:701418/128‑1 (MQ=255)
                                                          atCTTAAAATTTTTATCCTCGTGATATATTTTTTTATTTGTTTATATTA‑ACAAAA‑AAAAAAAAAAAAATCCCCTTAAAAAT‑TAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCtt                                                                    <  1:4446275/127‑1 (MQ=255)
                                                                   tttAGATACTCTTTATCTATTTTTTTATTTTTTTATCTTA‑AAAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt                                                       <  1:5099626/126‑1 (MQ=255)
                                                                        ttacttgttttCTTTTTTTTTATATTTTTATATTA‑AAAAAA‑AAAAAAAAAAAAATACCC‑‑TTAAA‑‑AAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                  <  2:5253125/118‑1 (MQ=255)
                                                                        atacattttatatatttttttattttttTATAATA‑AAAAAA‑AAAAAAAAAAAAATACCC‑‑TTAAA‑‑AAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                 <  1:1859498/116‑1 (MQ=255)
                                                                         ttcaagtttattatttttttattttttTAAATTT‑AAAAAA‑AAAAAAAAAAAAATACCC‑TTAAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAc                                                   <  1:4889830/117‑1 (MQ=255)
                                                                           ctacttatttatttttttattttttTAAAATA‑AAAAAA‑AAAAAAAAAAAAAAACCC‑CATAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                 <  1:4797826/119‑1 (MQ=255)
                                                                           atagttaTCTATTTTTTTTTTTTTTTATATTA‑AAAAAA‑AAAAAAAAAAAAAACCCC‑ATAAAA‑‑ATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                 <  1:580521/122‑1 (MQ=255)
                                                                            ctcgcttatATTTTTTTAATT‑TTTTTAATA‑AAAAAA‑AAAAAAAAAAAAAAACCCCTTAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                <  2:4758260/120‑1 (MQ=255)
                                                                            actttatatATTTTTTTAATTTTTAATAATA‑AAAAAA‑AAAAAAAAAAAAAAACCC‑ATAAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                <  1:1570656/120‑1 (MQ=255)
                                                                             cttgttatATTTTTTTAATTTTTTATCTACAAAAAAA‑AAAAAAAAAACAATCCCC‑TTAAAA‑‑ATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                <  1:3812429/121‑1 (MQ=255)
                                                                              ttttaaattttttttatttttttaAAATA‑AAAAAA‑AAAAAAAAAAAAAAACCCTTAAAAAT‑TAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                <  1:3190585/120‑1 (MQ=255)
                                                                                ttattttttttttatttttttaATTTA‑AAAAAA‑AAAAAAAAAAAAATCCCC‑‑ATAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                           <  2:4486707/122‑1 (MQ=255)
                                                                                ttaaatattttttattttttaATATAA‑AAAAAA‑AAAAAAAAAAAAAACCC‑‑‑ATAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                          <  1:1009549/121‑1 (MQ=255)
                                                                                  tatttttttttatttttttaAATTT‑AAAAAT‑AAAAAAAAAAAAAATACCATTAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                           <  1:2768071/124‑1 (MQ=255)
                                                                                   atatttttttattttttTATATTA‑TAAAAT‑TAAAAAAAAAAAAAACAATT‑‑‑AA‑‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAAATCTATTTCTTAAAATTCCATATTATACCACattttatttt                                      <  2:4348882/127‑1 (MQ=255)
                                                                                    ttattttttATTTTTTTAAATTA‑AAAAAA‑AAAAAAAAAAAAAAAAC‑‑ATTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttattt                                       <  1:4094042/125‑1 (MQ=255)
                                                                                     tttttttttttttttTATATTA‑TAAAAT‑AAAAAAAAAACAAACACC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                      <  2:4994567/127‑1 (MQ=255)
                                                                                      tttttttATTT‑TTTATTTTT‑ATAAAA‑TATAAAAAAAAAATTCAC‑TTTAAT‑‑AATTAAAAAATATAAAAAAAAAAAACTAATAAGAAACTCTATTTCTTTAAATTCCCTATTATACCACATTTTAttttt                                     <  1:2426548/128‑1 (MQ=255)
                                                                                       ttttttattt‑tttaAATTT‑AAAAAA‑TAAAAAAAAACAAAACCAATAAAAA‑‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                     <  1:1325743/128‑1 (MQ=255)
                                                                                       ttttttATTTTTTTAATTTA‑AAAAAA‑AAAAAAAAAAAAATACCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                   <  1:5269872/128‑1 (MQ=255)
                                                                                       ttttttATTAGTTTATATTA‑AAAAAT‑AAAAAAAAAAAAATACAC‑TTAAAA‑‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                     <  1:1265012/128‑1 (MQ=255)
                                                                                        tttttATTTTTTTTTATTC‑A‑AAAA‑AAAAAAAAAAAAAAACACATTAAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                    <  2:5098867/128‑1 (MQ=255)
                                                                                            tATTTTTTTATATTA‑AAAAAT‑AAAAAAAAAAAAAAACCC‑‑ATAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTCCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATa                               <  2:4592541/128‑1 (MQ=255)
                                                                                              ttttttTATCATC‑AAAAAT‑AAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAg                             <  1:140793/128‑1 (MQ=255)
                                                                                               ttttttATATTA‑AAAAAT‑AAAAAAAAAAAAAAACCCTTTAAAA‑‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTATTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                              <  1:1221630/125‑1 (MQ=255)
                                                                                                  tttATCTTC‑ACAAATAAAAAAAAAAACAATCCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACAAAAAACAAACTATTTTTATTAAAATACCATATTTAGCCAAAATTTTTTTTTAAAATaaatat                          >  2:4702795/1‑124 (MQ=255)
                                                                                                  tttATCTTC‑ACAAAT‑AAAAAAAAAACAATCCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAA‑‑‑AAAAACAAAAAAAATTTATAAAAAATCCCTATTAAACAAAATTTTATTTTTAAAATaaaaaatataa                     >  2:3137384/1‑119 (MQ=255)
                                                                                                   ttATCTTA‑AAAAAA‑AAAAAAAAAACAATTCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATAtt                        <  1:4230090/128‑1 (MQ=255)
                                                                                                     ttttttaataaaT‑AAAAAAAAAAAAAAACCCTT‑‑‑AA‑‑AAAT‑TTAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGAc                     <  2:50218/119‑1 (MQ=255)
                                                                                                      attataaaaaaT‑AAAAAAAAAAAAATTCAC‑‑TTAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                      <  1:1125477/120‑1 (MQ=255)
                                                                                                       aatC‑AAAAAT‑AAAAAAAAAATAATACAC‑‑TTAAA‑‑AATTAAAAAAAATAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTTAAATAAGATATTGACa                    <  2:2875334/126‑1 (MQ=255)
                                                                                                        ttC‑ACAAA‑‑AAAAAAAAAAAAAAAACCATAAAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                    <  2:5530307/128‑1 (MQ=255)
                                                                                                        ataaaaaaaa‑aaaaaaaaaaaaATCCC‑‑ATTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                    <  1:2354457/122‑1 (MQ=255)
                                                                                                           caaaaaa‑aaaaaaaaaaaaATACCC‑‑ATAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataa                 <  2:619309/125‑1 (MQ=255)
                                                                                                             acaaT‑AAAAAAAAAAAAATCCAC‑‑TTAAA‑‑AATTTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataata               <  2:4850009/126‑1 (MQ=255)
                                                                                                             aaaaa‑aaaaaaaaaaaaATAACAATTAAAAATAAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACat                   <  1:2963631/127‑1 (MQ=255)
                                                                                                             aaaaT‑AAAAAAAAAAAAAT‑ACATTTT‑AA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGatatatattatgtc               <  1:2130457/127‑11 (MQ=255)
                                                                                                               aataaaaaaaaaaaCAATCCCCTT‑‑‑AA‑‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatata             <  1:4976852/124‑1 (MQ=255)
                                                                                                                aT‑AAAAAAAAAAAAAT‑ACCATTAAAA‑‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatata             <  2:538324/128‑1 (MQ=255)
                                                                                                                   aaaaaaaaaaaaATACCATTAAAAA‑‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAatatat            <  1:2243260/128‑1 (MQ=255)
                                                                                                                      aaaaaaaaaaTCCCC‑‑TTAAA‑‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTa       <  2:1799571/128‑1 (MQ=255)
                                                                                                                        aaaaaCAATCACCTT‑‑‑AA‑‑AAATT‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAatat   <  1:1710258/128‑1 (MQ=255)
                                                                                                                                 |                                                                                                                             
AGTGAAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTC‑ACAAAT‑AAAAAAAAAACAATCCCCTTTTAAA‑‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATA  >  CP016816/202354‑202604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑