Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4850T.70.7% 425.8 / inf 471intergenic (+263/‑412)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (32/88);  new base . (117/172);  total (169/302)
Fisher's exact test for biased strand distribution p-value = 9.35e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-01

Only 100 of 510 total aligned reads displayed.

AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCCTTTTAAA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑ACATAATATATATCTAATATA  >  CP016816/202358‑202604
                                                                                                                                    |                                                                                                                         
 aGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTT‑GATCATCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AA‑AAA‑AAAAAAAAAATAACCtt                                                                                                                          >  2:2856203/1‑128 (MQ=255)
  tatAGTGTTAATAAATTTGTTAAGGTTAATTAAAAAACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑ATAAAA‑AAAAAAACAATCCCCtt                                                                                                                          <  2:2757219/126‑1 (MQ=255)
  gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCACCtaa                                                                                                                         >  2:1206941/1‑126 (MQ=255)
  gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCCtta                                                                                                                         <  1:1361606/128‑2 (MQ=255)
   ttAGTTTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTAAATAATTCTTATAATGTA‑AATCATCGTTAACTATATTTTTATTTATATAAATTAAC‑AA‑AT‑AAA‑AAAAAAAAAAAACCCttaa                                                                                                                        >  2:1901925/1‑126 (MQ=255)
    tAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑TATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCACCttaaa                                                                                                                       >  2:4524786/1‑125 (MQ=255)
     aGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATATA‑GATCATCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAAAAATCCCCtaaaaa                                                                                                                      >  2:5492843/1‑123 (MQ=255)
               ataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCTTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAAACCACTT‑‑‑AA‑AAATaaaaaaaa                                                                                                        >  2:4863054/1‑128 (MQ=255)
               ataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCCTT‑‑‑AA‑AAATaaaaaaaa                                                                                                        >  1:693732/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATACC‑‑ATTAAA‑AATTaaaaaaaa                                                                                                        >  2:592336/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTAATAATGTA‑GATCCTCGTGATCTATTTTATTATTTGTTTAACTTCAC‑AA‑AT‑AAA‑AAAAAAAAAAAACACTaaaaaa‑aaaaaaaaaa                                                                                                          >  2:2446205/1‑128 (MQ=255)
                taGAGTTAAGGTTAATTAAAATACAATAAACTTAATAATTAATATAATATA‑TATCATCTAAATCAATTTTATTATTAGTTAATATTAAA‑AA‑AT‑AAA‑AAAAAAAAAATCACATT‑‑‑AA‑AAATaaaaaaaaa                                                                                                       >  2:2011878/1‑128 (MQ=255)
                 agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAAT‑‑‑CAAATAAA‑AAATaaaaaaaaaa                                                                                                      >  1:606272/1‑128 (MQ=255)
                    gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAAAACCC‑‑TAAAA‑AAATaaaaaaaaaaaa                                                                                                    >  2:2134203/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCAAC‑‑‑TAAA‑AAATtaaaatataaaaaaaa                                                                                                >  2:4667784/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAAAAATACCC‑‑TAAAA‑AAATaaaaaaaaaaaaaaa                                                                                                 >  2:477425/1‑128 (MQ=255)
                       aaGGTTAATTAAAATACCATAAACTTAAAAATTCTTATAATGTA‑TATCCTAGTGATCTATTTTTTAATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAAAAAACACCTT‑‑‑AA‑AAATaaaaaaaaaaaaaaaa                                                                                                >  2:1716719/1‑128 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCTTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAAAAATAACC‑‑ATAAA‑AATTaaaaaaaaaaaaaaaaa                                                                                               >  2:1535112/1‑128 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAAACCCCTT‑‑‑AA‑AAATaaaaaaaaaaaaaaaaaa                                                                                              >  2:4417682/1‑128 (MQ=255)
                         ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATATGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCC‑‑ATAAA‑AATTaaaaaaaaaaaaaaaaa                                                                                               >  1:4400487/1‑128 (MQ=255)
                           ttaattaaAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAA‑AA‑AAA‑AAAAAAACAAAACAC‑‑ATAAA‑AATTaaaaaaaaaaaaaaaaaa                                                                                              >  1:4880710/1‑128 (MQ=255)
                            taattaaAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCC‑‑TTAAA‑AATTaaaaaaaaaaaaaaaaaaaa                                                                                            >  1:559498/1‑128 (MQ=255)
                            taattaaAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑‑AA‑AAAAAAACAAACCCC‑‑‑TAAA‑AAATaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  2:667139/1‑127 (MQ=255)
                            taattaaAAAAACAAAAAATTAATAAATATTATAATTTATAATCCCTGTTA‑ATATTTTTTTTATTTTTTAAAATAAA‑AA‑AA‑AAA‑AAAAAAAAAAACCCC‑‑TTAAA‑AATTaaaaaaaaaaaaaaaaaaaa                                                                                            <  1:4655357/128‑1 (MQ=255)
                             aattaaAATACCATAAACTTAATAATTATTATAATGAA‑GATAATATTGATCTATTATTTTATTTAATTATATTCAC‑AA‑AA‑AAA‑AAAAAAAAAAAAAAC‑‑TTAAA‑AAATaaaaaaaaaaaaaaaaaaaaa                                                                                           >  1:1795945/1‑128 (MQ=255)
                             aattaaAATACCATAAACTTAATAATACTTATAATGTA‑GATCCTCGTGATCTATTTATTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAAAAAACAACTTaaaaa‑aaaaaaaaaaaaaaaaaaaaaaa                                                                                             >  2:1144124/1‑128 (MQ=255)
                              attaaAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCTTTATCTATTTTTTTATTTTTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAAT‑‑ACAATTAAA‑AATTaaaaaaaaaaaaaaaaaaaaaa                                                                                          >  1:3871012/1‑127 (MQ=255)
                              attaaAATACAATAAAATTATTAATTATTTTAATTTA‑TATCATCTGGTTTTATTTTTTTATTTTTTTATTTTAAA‑AA‑AT‑AAA‑AAAAAAAAAAATCCC‑CTTAAA‑AATTaaaaaaaaaaaaaaaaaaact                                                                                           <  2:1364846/128‑3 (MQ=255)
                               ttaaAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCC‑‑‑TAAA‑AAATaaaaaaaaaaaaaaaaaaaaaaaa                                                                                        >  1:5013223/1‑125 (MQ=255)
                                 aaaaTAACTTAAAATTAATAATTATTTAAATTTA‑GAACCTTATTTTCTATTTTTTTATTTTTTTATAATCAA‑AA‑AA‑AAA‑AAAAAAAAAATCCCC‑‑TTAAA‑AAAT‑AAAAAAAAAAAAAAAAAAAACTaaa                                                                                      <  1:3429097/128‑1 (MQ=255)
                                  aaaTACAAAAAAATTAAAAATTATTATAATTTA‑GATACTCCTTATCTATTTTTTTATTTTTTTATATTAAA‑AA‑AT‑AAA‑AAAAAAACAATCCCC‑‑TTAAA‑AATTaaaaaaaaaaaaaaaaaactaaaaac                                                                                      <  1:4235615/128‑9 (MQ=255)
                                    aaaaaaTAAAATTAATAATAATTATAATTTT‑TTTAATTGTTATATATTTTTTTTTTTTTTTATATTAAA‑AA‑AT‑AAA‑AAAAAAAAAATCCCCTT‑‑‑AA‑AAATTAAAAAAAAAAAAAAAAAAAACTAAaaac                                                                                   <  1:28440/123‑1 (MQ=255)
                                     tacataaaaaataataaTTTTAATAATATA‑CAAACTCCTGAACTTTTTTTTTATTTTTTTATATTAAA‑AA‑AA‑AAA‑AAAAAAAAAATACCCTT‑‑‑AA‑AAATTAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                  <  1:1076287/117‑1 (MQ=255)
                                       ccATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAAAAATACCC‑‑TTAAA‑AATTAAAAAAAAAAAAAAAAAAACAaaaaaaaaaa                                                                                 >  2:3640120/1‑128 (MQ=255)
                                       ccATAAAATTAATAATTAATTTAATTTA‑CATACTCTTTTTATTTTTTTTTAATAGTTTATCATAAA‑AA‑AA‑AAA‑AAAAAAACAAACCCC‑‑TTAAA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                 <  1:3821801/128‑1 (MQ=255)
                                        cATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCC‑‑TTAAA‑AATTAAAAAAAAAAAAAAAAAAAATAaaaaaaaaaa                                                                                >  2:4303522/1‑128 (MQ=255)
                                        cATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCC‑‑ATAAA‑AATTAAAAAAAAAAAAAAAAAAAAA‑AAAaaacaaaa                                                                               >  2:5311911/1‑127 (MQ=255)
                                         aTAAACATAAAAAATTTTATAATTTA‑TATCCATGTGATTTATTTTTTTTTTTGTTTATATTAAA‑AA‑AA‑AAA‑AAAAAAAAAATCCCC‑‑TTAAA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAaaacaaac                                                                               <  1:4256050/128‑1 (MQ=255)
                                          tAAAATTAATAATTATTATAATTTA‑TTTAATTTTTTTATTTTTTTTTATTTTTTTATTTTAAA‑AA‑AT‑AAA‑AAAAAAAAAATACCATT‑‑‑AA‑AAAT‑‑TAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                           <  1:2530319/128‑1 (MQ=255)
                                            aaCTTAATAATTCTTATAATTTT‑TCTCCTCGTGATTAATTTTTTTATTTTTTTATATTAAA‑AA‑AT‑AAA‑AAAAAAACAATCCCCTT‑‑‑AA‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                           <  2:4154961/128‑1 (MQ=255)
                                             aattAATAATACTAATAATTTA‑GATTCTCTTTATAAATTTTTTTATTTTTTTATATTAAC‑AA‑AT‑AAA‑AAAAAAACAATCCCC‑‑TTAAA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                           <  1:471763/126‑1 (MQ=255)
                                              attAAAAATTATTAAAATTTA‑TATACTAGTTATCAATTTTTTTATTTTTTTAAATAAAA‑AA‑AA‑AAA‑AAAAAAAAAAACCCC‑TTAAAA‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                           <  1:5319665/127‑1 (MQ=255)
                                               ataattaTTTTTATAATGTT‑TATCATCTTTTTATATTTTTTTATTTTTTTATTTTCAA‑AA‑AT‑AAA‑AAAAAAACAATCCCCTT‑‑‑AA‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTAttt                                                                        <  1:4318745/127‑1 (MQ=255)
                                                 aataatTCTATAAATTTA‑CATCCACCTTATCTATTTTTTTATATGTTTATATTCAC‑AA‑AT‑AAA‑AAAAAAAAAAACCCC‑‑TTAAA‑AAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCtt                                                                     <  1:415515/128‑1 (MQ=255)
                                                   taaaTCTTATAATGTT‑TATACTCGTTATCTTATTTTTTATTTTTTTATCTTAAC‑AA‑AT‑AAA‑AAAAAAACAATCCCCTT‑‑‑AA‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTaa                                                                   <  1:4951351/128‑1 (MQ=255)
                                                                    tataattttGATTTATTTTTTTTTTTTTTTTACTACAA‑AA‑AA‑AAA‑AAAAAAAAAAAACACATT‑‑AA‑AAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAc                                                    <  1:4781232/120‑1 (MQ=255)
                                                                     atacattttatatatttttttattttttTATAATAAA‑AA‑AA‑AAA‑AAAAAAAAAATACCC‑‑TTAAA‑AAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                  <  1:1859498/116‑1 (MQ=255)
                                                                      tcaacttgttCTAATTTTTTATTTTTTTTTTTTAAA‑AA‑AA‑AAA‑AAAAAAAAAATACCCTTT‑‑AA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                   <  1:3635568/119‑1 (MQ=255)
                                                                      tacacttGATATATTTTTTTATTTTTTTATTTTTAA‑AA‑AA‑AAA‑AAAAAAAAAAAACCC‑‑TTAAA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                   <  2:4722187/122‑1 (MQ=255)
                                                                      acctagttttatatttttttattttttTATATTCAA‑AA‑AA‑AAA‑AAAAAAAAAATCCCC‑‑TTAAA‑ATTTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                   <  2:161917/117‑1 (MQ=255)
                                                                       actagttataaaTTTTTTTATTTGTTTTAATTAAA‑AA‑AA‑AAA‑AAAAAAAAAAAAACC‑‑ATAAA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                  <  1:1309505/117‑1 (MQ=255)
                                                                        cacttGATATATTTTTTTATTTTTTTAAATTAAA‑AA‑AA‑AAA‑AAAAAAAAAATACACTTAAAAA‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                   <  1:5474754/124‑1 (MQ=255)
                                                                        atttttatttatttttttattttttAATATTTAA‑AA‑AA‑AAA‑AAAAAAAAAATACCC‑TTAAAA‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                  <  1:93207/119‑1 (MQ=255)
                                                                         tcttcttCTATTTTATTATTTTGTTAT‑ATAAC‑AATAA‑AAA‑AAAAAAAAAATCCCC‑‑ATAAA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcaca                                                <  2:2627784/122‑1 (MQ=255)
                                                                         gcGTGATATATTTTTTTATTTGTTTATTTTAAC‑AA‑AA‑TAA‑AAAAAAACAATCCACTT‑‑‑AA‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACat                                               <  1:2117465/127‑1 (MQ=255)
                                                                         actttatatATTTTTTTAATTTTTAATAATAAA‑AA‑AA‑AAA‑AAAAAAAAAAAACCC‑ATAAAA‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                 <  1:1570656/120‑1 (MQ=255)
                                                                          ctgtttatatttttttattttttTATCTAAAA‑AA‑AA‑AAA‑AAAAAAAAAAAAACCCTT‑‑‑A‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                             <  2:459678/121‑1 (MQ=255)
                                                                          atttttatatttttttattttttTTTCTTAAA‑AA‑AA‑AAA‑AAAAAAAAAAACACATT‑‑‑AA‑AAAT‑AAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                             <  1:2736232/121‑1 (MQ=255)
                                                                           tttttatatttttttattttttTAAATAAAA‑AA‑AA‑AAA‑AAAAAAAAAATCCC‑‑ATTAAA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                              <  1:3738302/122‑1 (MQ=255)
                                                                           ttttaaattttttttatttttttaAAATAAA‑AA‑AA‑AAA‑AAAAAAAAAAAACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                 <  1:3190585/120‑1 (MQ=255)
                                                                           tttattattttttttatttttttaTATATA‑‑AA‑AA‑AAA‑AAAAAAATAAAAACCCTTTAAA‑ATTTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACat                                               <  1:1197292/120‑1 (MQ=255)
                                                                            tttttatattttttttttttttATATATAA‑AA‑AA‑AAA‑AAAAAAAAAAAACCCCTT‑‑AA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                             <  2:5221284/120‑1 (MQ=255)
                                                                             tttttatttttttattttttTATATTAAA‑AA‑AT‑AAA‑AAAAAAA‑AAAACACTTAAAAA‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                             <  1:1946059/124‑1 (MQ=255)
                                                                             ttattttttttttatttttttaATTTAAA‑AA‑AA‑AAA‑AAAAAAAAAATCCCC‑‑ATAAA‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                            <  2:4486707/122‑1 (MQ=255)
                                                                             ttaaatattttttattttttaATATAAAA‑AA‑AA‑AAA‑AAAAAAAAAAACCC‑‑‑ATAAA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                           <  1:1009549/121‑1 (MQ=255)
                                                                             tatttATTTTTTTTTTTTTTAATATTAAA‑AA‑AT‑AAA‑AAAAAAAAAAACCCCTT‑‑‑AA‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                           <  1:5052836/124‑1 (MQ=255)
                                                                               tatttttttttatttttttaAATTTAA‑AA‑AT‑AAA‑AAAAAAAAAAATACCATTAAAA‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                            <  1:2768071/124‑1 (MQ=255)
                                                                               tCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCCTT‑‑‑AA‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAAAAAAATATATTTATTAAAATACACATTTATAACAAatttttttt                                        >  2:2932287/1‑128 (MQ=255)
                                                                                tttttttttttttttttttTATTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCCTT‑‑‑AA‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                       <  2:560553/125‑1 (MQ=255)
                                                                                tattttttttATTT‑TTTATATTAAA‑AA‑ATAAAA‑AAAAAAACAATCCCCTTT‑‑AA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatt                                         <  1:4192937/125‑1 (MQ=255)
                                                                                atatttttttattttttTATATTATA‑AA‑AT‑TAA‑AAAAAAAAAAAACAATT‑‑‑AA‑AAAT‑TAAAAAAAAAAAAAAAAAAACTAAAAACAAAATCTATTTCTTAAAATTCCATATTATACCACattttatttt                                       <  2:4348882/127‑1 (MQ=255)
                                                                                  tttttttttttttttttTCTTAAC‑AA‑AT‑AAA‑AAAAAAATAATTACCTT‑‑‑AA‑AAATTTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                      <  1:143333/127‑1 (MQ=255)
                                                                                  tttttttttttttttTATATTATA‑AA‑AT‑AAA‑AAAAAAACAAACACC‑‑TTAAA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                       <  2:4994567/127‑1 (MQ=255)
                                                                                  ttttttttATTTTTTTTTTTTAAA‑AA‑AA‑AAA‑AAAAAAAAAAT‑‑ATAATTAAA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                       <  2:730982/127‑1 (MQ=255)
                                                                                  ttttttttATTTTTTTTTATAAAA‑AA‑AA‑AAA‑AAAAAAAAAATCCCC‑‑TTAAA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                       <  2:5106523/127‑1 (MQ=255)
                                                                                   tttttttatttttttaAATAAAA‑AA‑AT‑AAA‑AAAAAAA‑AAAAACCATTAAAA‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                       <  1:1261677/128‑1 (MQ=255)
                                                                                   tttttttATTT‑TTTATTTTTAT‑AA‑AA‑TAT‑AAAAAAAAAATTCAC‑TTTAAT‑AATTAAAAAATATAAAAAAAAAAAACTAATAAGAAACTCTATTTCTTTAAATTCCCTATTATACCACATTTTAttttt                                      <  1:2426548/128‑1 (MQ=255)
                                                                                   tatttttATTTTTTAATTTTAAA‑AA‑AA‑AAA‑AAAAAAAAAATACCC‑‑TTAAA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                      <  1:3972112/126‑1 (MQ=255)
                                                                                    ttttttttttttttATATT‑AA‑AA‑AA‑AAA‑AAAAAAAAAATAAACTT‑‑‑AA‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                   <  2:4793384/128‑1 (MQ=255)
                                                                                    ttttttATTTTTTTA‑CATAAA‑AA‑AA‑AAA‑AAAAAAAAAAAACCC‑ATAAAA‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                     <  1:4478782/128‑1 (MQ=255)
                                                                                     tttttATTTTTTAATATTAAA‑AA‑AA‑AAA‑AAAAAAAAAATCCCC‑‑TTAAA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaa                                    <  1:1920747/128‑1 (MQ=255)
                                                                                         tATTTTTTTATTTTAAA‑AA‑AA‑AAA‑AAAAAAAAAAT‑ACCATTAAAA‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaata                                 <  1:859031/128‑3 (MQ=255)
                                                                                           ttttttTATATTAAA‑AA‑AA‑AAA‑AAAAAAAAAATACCC‑‑TTAAA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAg                              <  1:5192166/128‑1 (MQ=255)
                                                                                              ttttATATTTAA‑AA‑AT‑AAA‑AAAAAAAAAATACCCTTAAAAA‑TTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGa                             <  1:1983488/127‑1 (MQ=255)
                                                                                               tttataattaaaaa‑aa‑aaa‑aaaaaaaaaaaACCC‑‑TTAAA‑AATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                           <  2:5408964/116‑1 (MQ=255)
                                                                                               tttATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCCTT‑‑‑AA‑AAATTAAAAAAAAAAAAAAAAAAA‑‑‑AAAAACAAAAAAAATTTATAAAAAATCCCTATTAAACAAAATTTTATTTTTAAAATaaaaaatataa                      >  2:3137384/1‑119 (MQ=255)
                                                                                                tttattataaaaa‑aT‑AAA‑AAAAAAAAAATCACC‑TTAAAA‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                           <  1:747226/117‑1 (MQ=255)
                                                                                                  ttttttaataa‑aT‑AAA‑AAAAAAAAAAAACCCTT‑‑‑AA‑AAAT‑TTAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑Ac                     <  2:50218/119‑1 (MQ=255)
                                                                                                   taatataaaa‑aT‑AAA‑AAAAAAAAAATACCC‑‑AAAAA‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑a                      <  2:4010623/120‑1 (MQ=255)
                                                                                                   attataaaaa‑aT‑AAA‑AAAAAAAAAATTCAC‑‑TTAAA‑AATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑a                      <  1:1125477/120‑1 (MQ=255)
                                                                                                    tcatccaaa‑aT‑AAA‑AAAAAAAAAAACCCC‑‑‑TAAT‑AAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑ACa                    <  2:3460609/121‑1 (MQ=255)
                                                                                                    tatttaaaa‑at‑aaatAAAAAAACAATACACTT‑‑‑AA‑AAATTTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑Ac                     <  1:2899516/121‑1 (MQ=255)
                                                                                                     ataaaaaa‑aa‑aaa‑aaaaaaaaaATCCC‑‑ATTAAA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑ACa                    <  1:2354457/122‑1 (MQ=255)
                                                                                                          aaa‑aa‑aaa‑aaaaaaaaaaaaaCCCTTAAAA‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAATTTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑ACataa                 <  1:515021/127‑1 (MQ=255)
                                                                                                          aaa‑aT‑AAA‑AAAAAAA‑AAAAACCATTAAAA‑ATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑ACataat                <  2:1837982/127‑1 (MQ=255)
                                                                                                               aaaaa‑aaaaaaaaaaaACCC‑‑TTAAA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑ACATAatatat            <  1:4521882/126‑1 (MQ=255)
                                                                                                                 aaa‑aaaaaaaaaaaACCCATTAAAA‑ATAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑ACATAatatat            <  1:4550311/128‑1 (MQ=255)
                                                                                                                   a‑aaaaaaaaaATCCCATTT‑‑‑A‑AAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑ACATAATATATATCTa       <  1:2222512/128‑1 (MQ=255)
                                                                                                                       aaaaaCAATCCCC‑‑TTAAA‑AATTAAAAAAAAAAAAAAAAAAAAA‑TAAAAACAAAATATAATTGAAAAAATTACATTTAATAAAAAATTTTTTTTTAAAAATAATATTTTGAATATAATAT‑TATATAatt    >  2:2310465/1‑127 (MQ=255)
                                                                                                                       aaaaaCAATCACCTT‑‑‑AA‑AAATT‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑ACATAATATATATCTAatat   <  1:1710258/128‑1 (MQ=255)
                                                                                                                                    |                                                                                                                         
AAGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTA‑GATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCAC‑AA‑AT‑AAA‑AAAAAAACAATCCCCTTTTAAA‑AAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTG‑ACATAATATATATCTAATATA  >  CP016816/202358‑202604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑