Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816202,4860T.54.7% 280.1 / inf 468intergenic (+264/‑411)JCVSYN2_00840/JCVSYN2_00845hypothetical protein/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (30/150);  new base . (98/119);  total (166/302)
Fisher's exact test for biased strand distribution p-value = 1.01e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-03

Only 100 of 500 total aligned reads displayed.

AGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATA  >  CP016816/202359‑202604
                                                                                                                                  |                                                                                                                      
 gTTATTGTAAATAATAGAGTTAAGGTTAATTAAAATACAACAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAACAATCCCCtta                                                                                                                       <  1:196011/128‑2 (MQ=255)
 gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATTATTCTTATAATGTAGATCATCATGATCTATTTTTTTAATTATTTATCTTAACAAATAAAAAAAAAACAAACCCCtta                                                                                                                       >  1:3640581/1‑127 (MQ=255)
 gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCtta                                                                                                                       <  1:1361606/128‑2 (MQ=255)
 gTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACACCtta                                                                                                                       >  1:4486707/1‑127 (MQ=255)
   tAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACCttaaa                                                                                                                     >  2:4524786/1‑125 (MQ=255)
     gtgtAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCCttaaaaa                                                                                                                   >  1:4436757/1‑123 (MQ=255)
              ataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCTTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCACTT‑‑‑AAAAATaaaaaaaa                                                                                                       >  2:4863054/1‑128 (MQ=255)
              ataGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATaaaaaaaa                                                                                                       >  1:693732/1‑128 (MQ=255)
               taGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATACC‑‑ATTAAAAATTaaaaaaaa                                                                                                       >  2:592336/1‑128 (MQ=255)
                agagTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAT‑‑‑CAAATAAAAAATaaaaaaaaaa                                                                                                     >  1:606272/1‑128 (MQ=255)
                   gTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTATATTAGATTATCTTCACAAATAAAAAAAAAACAAACCC‑‑‑ATAAAAAATaaaaaaaaaaaaa                                                                                                  >  2:1712390/1‑128 (MQ=255)
                      aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTATATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAAAAAACACCTT‑‑‑AAAAATaaaaaaaaaaaaaaaa                                                                                               >  2:5014241/1‑128 (MQ=255)
                      aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAACAATCAAC‑‑‑TAAAAAATtaaaatataaaaaaaa                                                                                               >  2:4667784/1‑128 (MQ=255)
                      aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTTTTTATCTTCACAAATAAAAAAAAAAAAATACCC‑‑TAAAAAAATaaaaaaaaaaaaaaa                                                                                                >  2:477425/1‑128 (MQ=255)
                      aaGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATATAGATCCTCGTTATCTATTTATTAATTTAATTATATTCAAAAATAAAAAAAAAACAAAACAC‑‑TAAAAAAATaaaaaaaaaaaaaaa                                                                                                >  2:291104/1‑128 (MQ=255)
                       aGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTAATCTATTTTTTTATTTTTTTATCTACAAAAATAAAAAAAAAACAAACAC‑‑ATAAAAAAATaaaaaaaaaaaaaaaa                                                                                               >  2:1197292/1‑128 (MQ=255)
                        ggTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCCCTT‑‑‑AAAAATaaaaaaaaaaaaaaaaaa                                                                                             >  2:4417682/1‑128 (MQ=255)
                        ccttaattaaAATACCATAAACTTAATAATTCTTATAATGTACATACACGAGAACTATTTTATAATTAGTTTATCTTAACAAAAAAAAAAAAAACAAAAAACTAaaaaaaaaaaaaaaaaaaaaaaaa                                                                                                >  2:2426548/3‑128 (MQ=255)
                           taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                           >  1:559498/1‑128 (MQ=255)
                           taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaa                                                                                           >  2:4351996/1‑128 (MQ=255)
                           taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAACAAACCCC‑‑‑TAAAAAATaaaaaaaaaaaaaaaaaaaaaa                                                                                         >  2:667139/1‑127 (MQ=255)
                           taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTATATCTTAACAAATAAAAAAAAAACAATCACC‑‑‑AAAAAAATtaaaaaaaaaaaaaaaaaaaa                                                                                          >  1:399708/1‑128 (MQ=255)
                           taattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTAATAGTTTATCTTCACAAATAAAAAAAAAACAATCAC‑‑‑ATAAAAAATtaaaaaaaaaaaaaaaaaaaa                                                                                          >  1:2752651/1‑128 (MQ=255)
                            aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCACC‑‑TAAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                          >  2:1305061/1‑128 (MQ=255)
                            aattaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAACAAACAC‑‑ATAAAAAATtaaaaaaaaaaaaaaaaaaaa                                                                                          >  2:5280482/1‑128 (MQ=255)
                            aattaaAATAAAATAAAATTATAAATTTTTATAATTTTCTTACTCGTTATTTTTTTTTTTATTTTTTTATATTTAAAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaa                                                                                          <  1:2870928/128‑1 (MQ=255)
                             attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaaaaaa                                                                                         >  1:2791216/1‑127 (MQ=255)
                             attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAAACCC‑‑‑ATAAAAAATaaaaaaaaaaaaaaaaaaaaaaa                                                                                        >  2:2612819/1‑126 (MQ=255)
                             attaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATATATTTATCTTCACAAATAAAAAAAAAACAAACACATaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                           >  2:1983488/1‑128 (MQ=255)
                             attaaAATACCATAAACTTAATAATTCTTACAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATCCC‑‑ATTAAAAATTaaaaaaaaaaaaaaaaaaaaaa                                                                                         >  1:5061399/1‑127 (MQ=255)
                              ttaaAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCAC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaa                                                                                        >  1:4303293/1‑126 (MQ=255)
                               taaAAAACAAAAAACTTAAAAATTCTTATAATTGATATCCTCGTGATCTATTTTTTTATTTGTTTAAATTCACAAAAAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaactaaa                                                                                       <  2:5105303/128‑6 (MQ=255)
                                aaaaaaaaaTAAAATTAATAATTATTATAATTTATATACTCTTTATATATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTaa                                                                                      <  1:262711/120‑1 (MQ=255)
                                aaaaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                      >  1:4726450/1‑124 (MQ=255)
                                  aaTACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTAACAAATAAAAAAAAAACAATCCCC‑‑TTAAAAATTAaaaaaaaaaaaaaaaaaaaaaaaaaa                                                                                    >  2:2851447/1‑128 (MQ=255)
                                    tacataaaaaataataaTTTTAATAATATACAAACTCCTGAACTTTTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                 <  1:1076287/117‑1 (MQ=255)
                                    taacATAAAATTAATAATTCTTATAATGTATATCCTCGTGATCTATTTTTTTTTTTTTTTATATTAAAAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                <  1:4529460/125‑1 (MQ=255)
                                     aacATAAAATTATTAATTTTTATAATTTTTATCCTATTTTTTTATTTTTTTATTTTTTTATATTAAAAAATAAAAAAAAAACAAAACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAaaaca                                                                                 <  2:4173594/126‑1 (MQ=255)
                                      ccATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAAAAATACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAACAaaaaaaaaaa                                                                                >  2:3640120/1‑128 (MQ=255)
                                      caaaaaaaTTAATTATTATTATAATTTACATACACGTGTATTATTTTTTTATTTTTTTATATTAAAAAAAAAAAAAAAAACAAAACCC‑‑ATAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                <  1:2403585/124‑1 (MQ=255)
                                      acATAAACTTAATAAATCTTAAAATGTACAACCACGTTACCTTTTTTTTTATTTTTTTATATTAACAAAAAAAAAAAAAAAAAACCCC‑‑ATAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                <  1:2317762/127‑1 (MQ=255)
                                       aaTAAAATTAAAAATTATTATAATTTATATCCTCTTTTTATATTTTTTTTTTTTTTTATCTTAAAAAAAAAAAAAAAAAAAAAACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAaaacaa                                                                                <  2:1883790/127‑1 (MQ=255)
                                        ataaaaaaaataatTATTAAAAATTATATCCTCGTGATCTATTTTTTTATTTTTTTATATTCACAAAAAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAAAAAActc                                                                            <  1:3837453/120‑1 (MQ=255)
                                          aaaattaaaaattaTTAAAATTTAAATACATTTTATATATTTTTTTTTTTTTTTAAATTAAAAAAAAAAAAAAAAAAAAAACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                          <  1:1887650/128‑1 (MQ=255)
                                          aaaaTTAATAATTATAATAATTTATTACATTGTTATTAATTTTTTTATTTTTTTAAATTAAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTa                                                                          <  2:1466451/128‑1 (MQ=255)
                                          aaaCTTAAAAATTTATATAATTTAGATACTCTTTATCTATTTTTTTAATTGTTTATATTCAAAAAAAAAAAAAAAAAAAACCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                           <  1:1844438/128‑1 (MQ=255)
                                             cTTAATATATTTTATTATTTATATACTCGTGATTTATTTTTTTTATTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAATCCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAActct                                                                           <  2:4306682/128‑1 (MQ=255)
                                                               ttataTCCTTCTGATATAATTTTTTATTTTTTTATTTAAAAAAAAAAAAAAAAAAAAATCCC‑‑AATAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCtatt                                                       <  1:932586/124‑1 (MQ=255)
                                                               ttAGATCATAGTTATCTATTTTTTTATTTGTTTATATTCACAAAAAAAAAAAAAACAATCCCCTT‑‑‑AAAAAT‑‑‑TAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAc                                                   <  1:5155878/127‑1 (MQ=255)
                                                                    ttcaagtttattatttttttattttttTAAATTTAAAAAAAAAAAAAAAAAAATACCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAc                                                   <  1:4889830/117‑1 (MQ=255)
                                                                    tacacttGATATATTTTTTTATTTTTTTATTTTTAAAAAAAAAAAAAAAAAAAAACCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                  <  2:4722187/122‑1 (MQ=255)
                                                                     actagttataaaTTTTTTTATTTGTTTTAATTAAAAAAAAAAAAAAAAAAAAAAACC‑‑ATAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                 <  1:1309505/117‑1 (MQ=255)
                                                                      tcgatctttatttttttttatttttttaTAATAAAAAATAAAAAAAAAAAAAACCCC‑‑ATAAAATATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                <  2:2281433/116‑1 (MQ=255)
                                                                      ctacttatttatttttttattttttTAAAATAAAAAAAAAAAAAAAAAAAAAACCCCATAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACca                                                 <  1:4797826/119‑1 (MQ=255)
                                                                      cacttGATATATTTTTTTATTTTTTTAAATTAAAAAAAAAAAAAAAAAAAATACACTTAAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATAcc                                                  <  1:5474754/124‑1 (MQ=255)
                                                                       actttatatATTTTTTTAATTTTTAATAATAAAAAAAAAAAAAAAAAAAAAACCCATAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACcac                                                <  1:1570656/120‑1 (MQ=255)
                                                                       aattGATATATTTTTTTATTTTTTTAAATATACAAAAAAAAAAAAAAAAAACCC‑‑‑ATAAAAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                            <  1:5197218/125‑1 (MQ=255)
                                                                        ctttaTCTATTTATTTATTTTTATATATTAAAAAAAAAAAAAAAAAAAATACAC‑‑TTAAAAAAT‑‑AAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                            <  1:2454586/124‑1 (MQ=255)
                                                                        ctgtttatatttttttattttttTATCTAAAAAAAAAAAAAAAAAAAAAAAACCCTT‑‑‑AAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                            <  2:459678/121‑1 (MQ=255)
                                                                         tatatatatttttttattttttTAAATTAAAAAAAAAAAAAAAAACAAAACCATTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACat                                              <  2:2132695/122‑1 (MQ=255)
                                                                          tttattattttttttatttttttaTATATAAAAAAAAAAAAAAATAAAAACCCTTTAAAATTTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACat                                              <  1:1197292/120‑1 (MQ=255)
                                                                          tttacaaTTTTTTTATATTTTTTATTTAAAATTATAAAAAAAAAAAATTCCATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                            <  1:3791137/122‑1 (MQ=255)
                                                                           ttataaTTTTTTTAATTTTTTATAATTAAAAAAAAAAAAAAAATAAAATACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatt                                             <  1:2077079/123‑1 (MQ=255)
                                                                           tatttATTTTTTTATTTGTTTATATTAAAAAATAAAAAAAAAAAAAAACCTTTAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                            <  1:1926592/124‑1 (MQ=255)
                                                                            ttattttttttttatttttttaATTTAAAAAAAAAAAAAAAAAAAATCCCC‑‑ATAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                           <  2:4486707/122‑1 (MQ=255)
                                                                            ttatATTTTTTTTTTTTTTTAATAATAAAAATAAAAAAAAAAACAATACCCTTTAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattt                                            <  1:3910822/125‑1 (MQ=255)
                                                                            ttaaatattttttattttttaATATAAAAAAAAAAAAAAAAAAAAAACCC‑‑‑ATAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                          <  1:1009549/121‑1 (MQ=255)
                                                                            aTCTATTTTTTTATTTTTTTATATTCAAAAAAAAAAAAAAAAAAAACCCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                           <  1:4229137/128‑1 (MQ=255)
                                                                            aTCAATTTTTTTATTTTTTTAACTTAACAAAAAAAAAAAAAAACAATCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttt                                           <  1:4669371/128‑1 (MQ=255)
                                                                             tatatttttttattttttTATATTAACAAATAAAAAAAAAAAAATACCCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACatttta                                          <  1:4243380/126‑1 (MQ=255)
                                                                              ttatttttttattttttTATTTTAAAAAATAAAAAAAAAAAAATCCCCAT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttattt                                       <  1:4584659/127‑1 (MQ=255)
                                                                              taaTTTTTTTTTTTTTTTATCTTAAAAAATAAAAAAAAAAAAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                      <  2:5462566/126‑1 (MQ=255)
                                                                              atatttttttattttttTATATAAAAAAAAAAAAAAAAAAAATACCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttattt                                       <  1:3034319/127‑1 (MQ=255)
                                                                               tttttttttttttttttttTATTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                      <  2:560553/125‑1 (MQ=255)
                                                                                attttttttATTTTTTATATTAAAAAAAAAAAAAAAAAAAAACCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                      <  1:5378082/126‑1 (MQ=255)
                                                                                 tttttttttttttttttTCTTAACAAATAAAAAAAAAATAATTACCTT‑‑‑AAAAATTTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTAttttt                                     <  1:143333/127‑1 (MQ=255)
                                                                                 tttttttttttttttTATATTATAAAATAAAAAAAAAACAAACACC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                      <  2:4994567/127‑1 (MQ=255)
                                                                                 ttttttttATTTTTTTTTTTTAAAAAAAAAAAAAAAAAAAAT‑‑ATAATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                      <  2:730982/127‑1 (MQ=255)
                                                                                  tttttttatttttttaAATAAAAAAATAAAAAAAAAAAAAAACCATTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                      <  1:1261677/128‑1 (MQ=255)
                                                                                  tttttttATTTTTTATTTTTATAAAATAAAAAAAAAAAATACC‑‑ATTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                    <  1:1779405/128‑1 (MQ=255)
                                                                                  tttttttAATTTTTTTTATTAAAAAAAAAAAAAAAAAAAAAACCCTTAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACattttatttt                                      <  1:3540696/128‑1 (MQ=255)
                                                                                   ttttttttttttttATATTAAAAAAAAAAAAAAAAAAATAAACTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaa                                  <  2:4793384/128‑1 (MQ=255)
                                                                                    ttttttttttttTATTTTAAAAAATAAAAAAAAAAAATACCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaaa                                 <  1:4177424/128‑1 (MQ=255)
                                                                                    tttttaatttttaaTATTAAAAAAAAAAAAAAAAAAAATACCCTAAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTa                                    <  1:5014241/128‑1 (MQ=255)
                                                                                     ttttATTTTTTTAAATAAAAAAAAAAAAAAAAAAAAAACCC‑‑ATAAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAGAATTCCCTATTATACCACATTTTATTTTTaaaa                                 <  2:5394764/128‑1 (MQ=255)
                                                                                       tttttttttTATTTTTAAAAATAAAAAAAAAAAAATAACCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATaa                              <  2:2685580/125‑1 (MQ=255)
                                                                                        tATTTTTTTATTTTAAAAAAAAAAAAAAAAAAAATACCATTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTaaata                                <  1:859031/128‑3 (MQ=255)
                                                                                              tttATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAATAAAAAAAAAATATTTTTATTAAAATTCCATTTTAAACAAATTTTTATTTTTAATATaatttttta                       >  1:4952246/1‑121 (MQ=255)
                                                                                              tttATCTTCACAAATAAAAAAAAAACAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAA‑‑‑AAAAACAAAAAAAATTTATAAAAAATCCCTATTAAACAAAATTTTATTTTTAAAATaaaaaatataa                     >  2:3137384/1‑119 (MQ=255)
                                                                                               tttattataaaaaaTAAAAAAAAAAAAATCACCTTAAAAATTAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGata                          <  1:747226/117‑1 (MQ=255)
                                                                                                 tatattaaaaaaTAAAAAAAAAAAAATAACATT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                      <  1:324894/119‑1 (MQ=255)
                                                                                                  taatataaaaaTAAAAAAAAAAAAATACCC‑‑AAAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                      <  2:4010623/120‑1 (MQ=255)
                                                                                                  attataaaaaaTAAAAAAAAAAAAATTCAC‑‑TTAAAAATAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGa                      <  1:1125477/120‑1 (MQ=255)
                                                                                                   tcatccaaaaTAAAAAAAAAAAAAACCCC‑‑‑TAATAAAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                    <  2:3460609/121‑1 (MQ=255)
                                                                                                   aaaaaaaaaaaaaaaaaaaaaaaATCCCC‑‑TTAAAAAATAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACa                    <  2:5177988/121‑1 (MQ=255)
                                                                                                         aaaaaaaaaaaaaaaaaaaACCATTTAAAATTAAAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACataa                 <  2:1571910/127‑1 (MQ=255)
                                                                                                         aaaaTAAAAAAAAAAAAATACATTTTAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGatatatattatgtc               <  1:2130457/127‑11 (MQ=255)
                                                                                                                  aaaaaaaaaaTCCCC‑‑TTAAAAATTAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTa       <  2:1799571/128‑1 (MQ=255)
                                                                                                                     aaaaCAATCCCCTT‑‑‑AAAAATTAAAAAAAAAAAAAAAAAAAACTAAACCCAACCTCTTTTTCATAAATTAGACTATTTGACCACATGTTATTTTTAAAATAATATTTTGACATAATATAAAAATAatat   >  1:4332088/1‑128 (MQ=255)
                                                                                                                       aaaaaataacctttaaaatttaaaaAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAata    <  1:169978/117‑1 (MQ=255)
                                                                                                                                  |                                                                                                                      
AGTTAGTGTAAATAATAGAGTTAAGGTTAATTAAAATACCATAAACTTAATAATTCTTATAATGTAGATCCTCGTGATCTATTTTTTTATTTGTTTATCTTCACAAATAAAAAAAAAACAATCCCCTTTTAAAAAATAAAAAAAAAAAAAAAAAAAAACTAAAAACAAACTCTATTTCTTAAAATTCCCTATTATACCACATTTTATTTTTAAAATAAGATATTGACATAATATATATCTAATATA  >  CP016816/202359‑202604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: